Antiinflammatory Effects of Korean Propolis in Lipopolysaccharide-stimulated RAW 264.7 Macrophages and PMA-induced Mouse Ear Edema

2019 ◽  
Vol 81 (3) ◽  
Author(s):  
S. H Kim ◽  
M. O Kim ◽  
E. K Kim ◽  
K. R Kim
Keyword(s):  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Ear Edema ◽  
Korean Propolis ◽  
Mouse Ear ◽  
Antiinflammatory Effects ◽  
Mouse Ear Edema

Anti-Inflammatory Activity of Dichloromethane Fraction from Katsuwonus pelamis Heart in LPS-Induced RAW 264.7 Cells and Mouse Ear Edema

2017 ◽  
Vol 45 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Min-Ji Kim ◽  
Nan-Young Bae ◽  
Hyeun-Deok Choi ◽  
Koth-Bong-Woo-Ri Kim ◽  
Sun-Hee Park ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Katsuwonus Pelamis ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Hexane Fraction from Sargassum fulvellum Inhibits Lipopolysaccharide-Induced Inducible Nitric Oxide Synthase Expression in RAW 264.7 Cells via NF-κB Pathways

2013 ◽  
Vol 41 (03) ◽  
pp. 565-584 ◽  
Author(s):  
Wi-Gyeong Gwon ◽  
Min-Sup Lee ◽  
Jong-Soon Kim ◽  
Jae-Il Kim ◽  
Chi-Won Lim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hexane Fraction ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Ear Edema ◽  
Sargassum Fulvellum ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

Sargassum fulvellum (Turner) C. Agardh has been used to treat various inflammatory diseases, including lump, dropsy, swollen and painful scrotum, and urination problems for several centuries with no side effects. This study aims to investigate the anti-inflammatory effect of the hexane fraction of S. fulvellum (HFS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and phorbol 12-myristate 13-acetate (PMA)-induced mouse-ear edema. The anti-inflammatory activity of HFS in LPS-stimulated RAW 264.7 cells was investigated by assessing the inhibition of nitric oxide (NO) and pro-inflammatory cytokine production during Griess reaction and enzyme-linked immunosorbent assay (ELISA), respectively. The molecular mechanisms that underlie the anti-inflammatory action of HFS were investigated by analyzing the activation of transcription factor and its upstream signaling proteins. Additionally, an in vivo study of the anti-inflammatory effect of HFS was carried out using PMA-induced mouse-ear edema. HFS inhibited LPS-induced NO production in a dose-dependent manner and suppressed the expression of inducible NO synthase (iNOS) in the RAW 264.7 cells. Further, HFS reduced the production of pro-inflammatory cytokines in the LPS-stimulated RAW 264.7 cells. HFS significantly inhibited LPS-induced nuclear factor kappa B (NF-κB) transcriptional activity and NF-κB translocation into the nucleus by preventing degradation of inhibitor κB-α. Moreover, HFS inhibited the activation of Akt and mitogen-activated protein kinases (MAPKs) in the LPS-stimulated RAW 264.7 cells. Furthermore, HFS suppressed PMA-induced mouse-ear edema. The above data indicate that the anti-inflammatory effects of HFS on LPS-stimulated cells are associated with the suppression of NF-κB through the inhibition of MAPKs and Akt phosphorylation.

Anti-Inflammatory Effect of Wheat Germ Oil on Lipopolysaccharide-stimulated RAW 264.7 Cells and Mouse Ear Edema

2016 ◽  
Vol 44 (3) ◽  
pp. 236-245 ◽  
Author(s):  
Bo-Kyeong Kang ◽  
Min-Ji Kim ◽  
Da-Hyun Jeong ◽  
Koth-Bong-Woo-Ri Kim ◽  
Nan-Young Bae ◽  
...  
Keyword(s):  
Wheat Germ ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Wheat Germ Oil ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

scholarly journals Isolation of Inhibitors of TPA-Induced Mouse Ear Edema from Hoelen, Poria cocos.

1996 ◽  
Vol 44 (4) ◽  
pp. 847-849 ◽  
Author(s):  
Haruo NUKAYA ◽  
Hirokazu YAMASHIRO ◽  
Hirotatsu FUKAZAWA ◽  
Hitoshi ISHIDA ◽  
Kuniro TSUJI
Keyword(s):  
Poria Cocos ◽  
Ear Edema ◽  
Mouse Ear ◽  
Mouse Ear Edema

Antifungal, Anti-Inflammatory and Cytotoxic Effects of Zingiber cassumunar Gel

2018 ◽  
Vol 773 ◽  
pp. 360-364 ◽  
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon ◽  
Rattiporn Kaypetch
Keyword(s):  
Cell Culture ◽  
Antifungal Activity ◽  
Diffusion Method ◽  
Cytotoxic Effects ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Zingiber Cassumunar ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

This study aimed to investigate the antifungal, anti-inflammatory and cytotoxic effects of Zingiber cassumunar gel. The gel was prepared from essential oil of Zingiber cassumunar rhizome by the Thailand Institute of Scientific and Technological Research. Antifungal activity of the gel was firstly determined by the well diffusion method against Candida albicans ATCC 10238 and candida strain isolated from the patient’s lesion. Then, the Agar overlay technique was used to test the cytotoxicity of Z. cassumunar gel on mouse fibroblasts (ATCC clone 929) according to ISO 7405. For anti-inflammatory effect of the gel, TPA (carrageenan lambda type IV, 12-O-tetradecanoylphorbol-13- acetate)-induced mouse ear edema method was used. The results of well diffusion showed that Z. cassumunar gel was quite a potent antifungal agent against both strains of tested C. albicans with inhibition zones of 12-13 mm. In the cytotoxicity test, the gel exhibited no toxicity to cell culture. In addition, topical administration of Z. cassumunar gel could decrease mouse ear edema induced by TPA. At 30 and 60 min-time points, Z. cassumunar gel showed higher anti-inflammatory activity than triamcinolone which was used as reference anti-inflammatory drug. In conclusion, gel prepared from Z. cassumunar oil showed antifungal activity against both strains of C. albicans. In addition, its anti-inflammatory effect was demonstrated within 30 min by the TPA-induced mouse ear edema model. The gel was non-toxic to cell culture after 24-h incubation. Further studies are needed to clarify the safety and benefit of this gel for clinical use in the treatment of candidal infection and inflammation.

scholarly journals Anti-Inflammatory Activity of Cnidoscolus aconitifolius (Mill.) Ethyl Acetate Extract on Croton Oil-Induced Mouse Ear Edema

2021 ◽  
Vol 11 (20) ◽  
pp. 9697
Author(s):  
Eduardo Padilla-Camberos ◽  
Omar Ricardo Torres-Gonzalez ◽  
Ivan Moises Sanchez-Hernandez ◽  
Nestor Emmanuel Diaz-Martinez ◽  
Oscar Rene Hernandez-Perez ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inflammatory Activity ◽  
Therapeutic Effects ◽  
Ear Edema ◽  
Croton Oil ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Nowadays, there is a growing interest in the development of medicinal plant-based therapies to diminish the ravages of the inflammatory process related to diseases and tissue damage. Most therapeutic effects of these traditional medicinal plants are owed to their phenolic and antioxidant properties. C. aconitifolius is a traditional medicinal plant in Mexico. Previous characterization reports have stated its high nutritional and antioxidant components. The present study aimed to better understand the biological activity of C. aconitifolius in inflammation response. We developed an ethyl acetate extract of this plant to evaluate its anti-inflammatory capacity and its flavonoid content. The topical anti-inflammatory effect of the ethyl acetate extract of C. aconitifolius was determined by the croton oil-induced mouse ear edema test, while flavonoid detection and concentration were determined by thin layer chromatography and the aluminum chloride colorimetric assay, respectively. Topical application of the extract showed significant inhibition of the induced-ear edema (23.52 and 49.41% for 25 and 50 mg/kg dose, respectively). The extract also exhibited the presence of flavonoids. The finding of the anti-inflammatory activity exerted by the C. aconitifolius and the identification of its active principles may suggest and support its use for inflammation treatment.

scholarly journals Ethanolic Extract of Origanum vulgare Suppresses Propionibacterium acnes-Induced Inflammatory Responses in Human Monocyte and Mouse Ear Edema Models

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1987 ◽  
Author(s):  
Lu-Te Chuang ◽  
Tsung-Hsien Tsai ◽  
Tsung-Jung Lien ◽  
Wen-Cheng Huang ◽  
Jun-Jen Liu ◽  
...  
Keyword(s):  
Propionibacterium Acnes ◽  
Inflammatory Responses ◽  
Acne Vulgaris ◽  
Suppressive Effect ◽  
Transcriptional Level ◽  
Origanum Vulgare ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Acne vulgaris (acne) is a common inflammatory skin disorder, and Propionibacterium acnes plays a major role in the development and progression of acne inflammation. Herbs possessing antimicrobial and anti-inflammatory activity have been applied as a medical option for centuries. In this study, we examined the suppressive effect of ethanolic oregano (Origanum vulgare) extract on live P. acnes-induced in vivo and in vitro inflammation. Following ethanol extraction of oregano leaves, four compounds with strong antioxidant activity, including rosmarinic acid, quercetin, apigenin, and carvacrol, were identified by high-performance liquid chromatography. Using the mouse ear edema model, we demonstrated that ethanol oregano extracts (EOE) significantly suppressed P. acnes-induced skin inflammation, as measured by ear thickness (32%) and biopsy weight (37%). In a separate study, using the co-culture of P. acnes and human THP-1 monocytes, EOE reduced the production of interleukin (IL)-8, IL-1β and tumor necrosis factor (TNF)-α up to 40%, 37%, and 18%, respectively, as well as the expression of these three pro-inflammatory mediators at the transcriptional level. Furthermore, EOE inhibited the translocation of nuclear factor-kappa B (NF-κB) into the nucleus possibly by inactivating toll-like receptor-2 (TLR2). The suppressive effect of EOE on live P. acnes-induced inflammatory responses could be due, in part, to the anti-inflammatory and antioxidant properties, but not the anti-microbial effect of EOE.

Anti-Inflammatory Effect of Sargassum patens C. Agardh Ethanol Extract in LPS-induced RAW264.7 Cells and Mouse Ear Edema

2017 ◽  
Vol 45 (2) ◽  
pp. 110-117 ◽  
Author(s):  
Min-Ji Kim ◽  
Min-Ju Kim ◽  
Koth Bong Woo Ri Kim ◽  
Sun Hee Park ◽  
Hyeun Deok Choi ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Raw264.7 Cells ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Sargassum Patens ◽  
Mouse Ear Edema ◽  
Inflammatory Effect
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