Inkjet Printed Blue-to-Green Color Conversion Layer Formation by OTS Treatment for QD-OLED Fabrication

Author(s):  
Kwan Hyun Cho ◽  
Young Joon Han ◽  
Kwan Hyun Cho
2020 ◽  
Vol 84 ◽  
pp. 105814
Author(s):  
Do Yeob Kim ◽  
Young Joon Han ◽  
Jun Choi ◽  
Chun Sakong ◽  
Byeong-Kwon Ju ◽  
...  

Author(s):  
Áron Pánczél ◽  
Simon P. Nagy ◽  
János Farkas ◽  
Zoltán Jakus ◽  
Dávid S. Győri ◽  
...  

Osteoclasts are multinucleated cells of hematopoietic origin which are critically involved in physiological and pathological bone resorption. They develop from myeloid progenitors through characteristic gene expression changes and intercellular fusion. This process is directed by M-CSF and RANKL which are also able to trigger osteoclast development from bone marrow cells in vitro. Osteoclasts are conventionally visualized by histochemical staining followed by manual counting, which hinders kinetic studies and automated quantification. Here we describe two fluorescence-based assays for the real-time analysis of myeloid cell to osteoclast development (FRAMCO) in primary mouse bone marrow cell cultures. Both assays rely on red-to-green fluorescence conversion of the membrane-targeted tdTomato/membrane-targeted eGFP (mTmG) transgene by Cre recombinase driven by the osteoclast-specific cathepsin K promoter (Ctsk-Cre). In the first assay (FRAMCO1.1), osteoclast-specific gene expression triggers red-to-green color conversion of cells carrying both the Ctsk-Cre and mTmG transgenes. In the second assay (FRAMCO1.2), red-to-green fluorescence conversion is triggered by fusion of neighboring co-cultured bone marrow cells separately carrying either the Ctsk-Cre or the mTmG transgenes. The two assays were tested using a high-content confocal fluorescence imaging system, followed by automated quantification. The FRAMCO1.1 assay showed robust red-to-green fluorescence conversion of more than 50% of the culture (including mononuclear cells) within 3 days under osteoclastogenic conditions. The FRAMCO1.2 assay showed a less robust but still readily measurable red-to-green color conversion in multinuclear cells within 5 days of differentiation. The assays required both the Ctsk-Cre and the mTmG transgenes and gave no signals in parallel macrophage cultures. The proper functioning of the two assays was also confirmed at the DNA, mRNA and bulk protein level. The assay systems were validated using lisophosphatidylcholine, a previously reported inhibitor of preosteoclast fusion. Taken together, our assays allow high-throughput automated real-time analysis of two critical aspects of osteoclast development, facilitating the screening for novel drug candidates for the pharmacological control of osteoclast-mediated bone resorption.


2021 ◽  
pp. 161301
Author(s):  
Fucai Xu ◽  
Haisheng Yang ◽  
Yujie Zhang ◽  
Xiaodong Liu ◽  
Yaqian Zhang ◽  
...  

Holzforschung ◽  
2000 ◽  
Vol 54 (3) ◽  
pp. 327-330 ◽  
Author(s):  
Shang-Tzen Chang ◽  
Jyh-Horng Wu

SummaryProcessed bamboo culms capable of maintaining the greenish color in their skin have increased economic value. The use of protectors to preserve the green color and to improve the color fastness of bamboo culms has, therefore, attracted great interest in the industry. In this paper three exposure tests, including accelerated UV lightfastness, outdoor weathering, and indoor exposure, were used to evaluate the color fastness of ma bamboo (Dendrocalamus latiflorusMunro) treated with chromium based reagents. Experimental results revealed that bamboo culms treated with CCP (chromated copper phosphate) or CP (chromated phosphate), both homemade by the authors, exhibited excellent color fastness. Among all inorganic salt-treated bamboo culms the CP-treated samples had the brighter greenish skin and also provided the best green color fastness. It was further noticed that ultraviolet light plays an important role on the color conversion of CrO3-treated bamboo. The green color becomes significantly enhanced with increased exposure time.


1990 ◽  
Vol 87 ◽  
pp. 1597-1607 ◽  
Author(s):  
L Benedetti ◽  
M Borsari ◽  
C Fontanesi ◽  
G Battistuzzi Gavioli

2020 ◽  
Author(s):  
◽  
Linas Sinkevičius
Keyword(s):  

Investigation of polypyrrole layer formation peculiarities and formation of uric acid molecular imprints


2009 ◽  
Vol 129 (4) ◽  
pp. 686-695 ◽  
Author(s):  
Sho Yokota ◽  
Akinori Sasaki ◽  
Hiroshi Hashimoto ◽  
Yasuhiro Ohyama

2017 ◽  
Vol 5 (3) ◽  
pp. 20
Author(s):  
JEBISHA J ◽  
MONISHA V ◽  
JEMI B. FEMILA ◽  
◽  
◽  
...  

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