scholarly journals PHENOTYPIC CHARACTERIZATION OF METALLO-Β-LACTAMASE AMONG CARBAPENEM-RESISTANT ENTEROBACTERIACEAE IN SOUTHWESTERN NIGERIA AND ACCURACY IN MEANS OF DIAGNOSIS

2019 ◽  
Keyword(s):  
Phenotypic Characterization ◽  
Southwestern Nigeria ◽  
Carbapenem Resistant ◽  
Carbapenem Resistant Enterobacteriaceae

scholarly journals Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae in the USA, 2011–2015

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S179-S179 ◽  
Author(s):  
Uzma Ansari ◽  
Adrian Lawsin ◽  
Davina Campbell ◽  
Valerie Albrecht ◽  
Gillian McAllister ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Carbapenem Resistant ◽  
The Usa ◽  
Carbapenem Resistant Enterobacteriaceae

Detailed characterization of an IncFII plasmid carrying blaOXA-48 from Lebanon

2020 ◽  
Vol 75 (9) ◽  
pp. 2462-2465 ◽  
Author(s):  
Jennifer Moussa ◽  
Balig Panossian ◽  
Elie Nassour ◽  
Tamara Salloum ◽  
Edmond Abboud ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Complete Sequence ◽  
Conjugative Plasmid ◽  
Incompatibility Group ◽  
Carbapenem Resistant ◽  
Class D ◽  
Long Read ◽  
Important Challenge ◽  
Carbapenem Resistant Enterobacteriaceae

Abstract Background The spread of carbapenem-resistant Enterobacteriaceae is an important challenge and an increasing healthcare problem. OXA-48 is a class D carbapenemase that is usually localized on a conjugative plasmid belonging to the IncL incompatibility group. Methods In this study, we used a combination of short- and long-read WGS approaches and molecular typing techniques to characterize the genetic environment of the smallest reported 27 029 bp IncFII plasmid carrying blaOXA-48 (pLAU-OXA48). Results The plasmid recovered from a clinical Escherichia coli isolate was positive for blaOXA-48, which was located within the Tn6237 composite transposon. Primers targeting junctions between the IncF fragment and Tn6237 for the rapid identification of pLAU-OXA48-like plasmids were designed. Conclusions To our knowledge, this is the first report showing the complete sequence of an IncFII plasmid carrying blaOXA-48 within Tn6237 using hybrid assembly of long- and short-read sequencing.

scholarly journals Characterization of Carbapenem-Resistant Enterobacteriaceae Cultured From Retail Meat Products, Patients, and Porcine Excrement in China

2021 ◽  
Vol 12 ◽  
Author(s):  
Jie Feng ◽  
Qian Xiang ◽  
Jiangang Ma ◽  
Pei Zhang ◽  
Kun Li ◽  
...  
Keyword(s):  
Meat Products ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Retail Meat ◽  
Different Origins ◽  
Different Sources ◽  
Carbapenem Resistant Enterobacteriaceae

The emergence and dissemination of carbapenem-resistant Enterobacteriaceae (CRE) is a growing concern to animal and public health. However, little is known about the spread of CRE in food and livestock and its potential transmission to humans. To identify CRE strains from different origins and sources, 53 isolates were cultured from 760 samples including retail meat products, patients, and porcine excrement. Antimicrobial susceptibility testing was carried out, followed by phylogenetic typing, whole-genome sequencing, broth mating assays, and plasmids analyses. Forty-three Escherichia coli, nine Klebsiella pneumoniae, and one Enterobacter cloacae isolates were identified, each exhibiting multidrug-resistant phenotypes. Genetically, the main sequence types (STs) of E. coli were ST156 (n = 7), ST354 (n = 7), and ST48 (n = 7), and the dominant ST of K. pneumoniae is ST11 (n = 5). blaNDM–5 (n = 40) of E. coli and blaKPC–2 (n = 5) were the key genes that conferred carbapenem resistance phenotypes in these CRE strains. Additionally, the mcr-1 gene was identified in 17 blaNDM-producing isolates. The blaNDM–5 gene from eight strains could be transferred to the recipients via conjugation assays. Two mcr-1 genes in the E. coli isolates could be co-transferred along with the blaNDM–5 genes. IncF and IncX3 plasmids have been found to be predominantly associated with blaNDM gene in these strains. Strains isolated in our study from different sources and regions tend to be concordant and overlap. CRE strains from retail meat products are a reservoir for transition of CRE strains between animals and humans. These data also provide evidence of the dissemination of CRE strains and carbapenem-resistant genes between animal and human sources.

scholarly journals Plasmidome analysis of carbapenem-resistant Enterobacteriaceae isolated in Vietnam

2020 ◽  
Author(s):  
Aki Hirabayashi ◽  
Koji Yahara ◽  
Satomi Mitsuhashi ◽  
So Nakagawa ◽  
Tadashi Imanishi ◽  
...  
Keyword(s):  
Carbapenem Resistance ◽  
Genomic Epidemiology ◽  
Carbapenem Resistant ◽  
Oxford Nanopore ◽  
Carbapenemase Gene ◽  
Long Read ◽  
Severe Infections ◽  
Oxford Nanopore Technologies ◽  
Carbapenem Resistant Enterobacteriaceae

Carbapenem-resistant Enterobacteriaceae (CRE) represent a serious threat to public health due to limited management of severe infections and high mortality. The rate of resistance of Enterobacteriaceae isolates to major antimicrobials, including carbapenems, is much higher in Vietnam than in Western countries, but the reasons remain unknown due to the lack of genomic epidemiology research. A previous study suggested that carbapenem resistance genes, such as the carbapenemase gene bla NDM-1 , spread via plasmids among Enterobacteriaceae in Vietnam. In this study, we performed detection and molecular characterization of bla NDM-1 -carrying plasmids in CRE isolated in Vietnam, and identified several possible cases of horizontal transfer of plasmids both within and among species of bacteria. Twenty-five carbapenem-resistant isolates from Enterobacteriaceae clinically isolated in a reference medical institution in Hanoi were sequenced on Illumina short-read sequencers, and 12 isolates harboring bla NDM-1 were sequenced on an Oxford Nanopore Technologies long-read sequencer to obtain complete plasmid sequences. Most of the plasmids co-carried genes conferring resistance to clinically relevant antimicrobials, including third-generation cephalosporins, aminoglycosides, and fluoroquinolones, in addition to bla NDM-1 , leading to multidrug resistance of their bacterial hosts. These results provide insight into the genetic basis of CRE in Vietnam, and could help control nosocomial infections.

scholarly journals Characterization of Carbapenem-Resistant Enterobacteriaceae with High Rate of Autochthonous Transmission in the Arabian Peninsula

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0131372 ◽  
Author(s):  
Ágnes Sonnevend ◽  
Akela A. Ghazawi ◽  
Rayhan Hashmey ◽  
Wafaa Jamal ◽  
Vincent O. Rotimi ◽  
...  
Keyword(s):  
Arabian Peninsula ◽  
High Rate ◽  
Carbapenem Resistant ◽  
Autochthonous Transmission ◽  
Carbapenem Resistant Enterobacteriaceae
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