scholarly journals Antifungal activity of hydroxytyrosol enriched extracts from olive mill waste against Verticillium dahliae, the cause of Verticillium wilt of olive

2021 ◽  
Vol 60 (1) ◽  
pp. 139-147
Author(s):  
Mounira Inas DRAIS ◽  
Elisa PANNUCCI ◽  
Rocco CARACCIOLO ◽  
Roberta BERNINI ◽  
Annalisa ROMANI ◽  
...  

Verticillium wilt (caused by Verticillium dahliae Kleb.) is an important disease affecting olive (Olea europaea L.) production. Effective control of this disease relies on integrated management strategies. In vitro antifungal activity of two hydroxytyrosol (HTyr) enriched extracts (HTE1 and HTE2) obtained from olive mill waste products (OMWP) was assessed against V. dahliae. Inhibitory effects of pure HTyr as a standard, and HTE1 and HTE2 at different concentrations, were evaluated on mycelium growth and conidium germination of V. dahliae. Chemical characterization of HTE1 and HTE2 allowed identification and quantification of HTyr as the main constituent in both extracts along with other low molecular weight phenols. HTE1 showed a higher inhibitory activity in both growth and conidium germination of V. dahliae. At the tested concentrations, low antifungal effects of HTyr were observed. After 3 d, 1 mg mL-1 of HTE1 gave greater inhibition of mycelium growth than HTE2 or HTyr. After 24 h, HTE1 gave 55% inhibition of conidium germination, and HTyr and HTE2 both gave 37% inhibition. This study has demonstrated that phenolic compounds derived from OMWP have antifungal activity against V. dahliae, indicating the potential of these compounds for eco-friendly control of Verticillium wilt.

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Georgios I. Zervakis ◽  
Georgios Koutrotsios ◽  
Panagiotis Katsaris

Two-phase olive mill waste (TPOMW, “alperujo”) is a highly biotoxic sludge-like effluent of the olive-oil milling process with a huge seasonal production. One of the treatment approaches that has so far received little attention is the use of TPOMW as substrate for the cultivation of edible mushrooms. Fifteen fungal strains belonging to five species (Basidiomycota), that is,Agrocybe cylindracea,Pleurotus cystidiosus,P. eryngii,P. ostreatus, andP. pulmonarius, were evaluated for their efficacy to colonize media composed of TPOMW, which was used either raw or composted in mixtures with wheat straw in various ratios. Qualified strains exhibited high values of biological efficiency (e.g., 120–135% forPleurotusspp. and 125% forA. cylindracea) and productivity in subsequent cultivation experiments on substrates supplemented with 20–40% composted TPOMW or 20% raw TPOMW. Only when supplementation exceeded 60% for raw TPOMW, a negative impact was noted on mushroom yields which could be attributed to the effluent's toxicity (otherwise alleviated in the respective composted TPOMW medium). Earliness and mushroom size as well as quality parameters such as total phenolic content and antioxidant activity did not demonstrate significant differences versus the control wheat-straw substrate. The substrates hemicellulose content was negatively correlated with mycelium growth rates and yields and positively with earliness; in addition, cellulose: lignin ratio presented a positive correlation with mycelium growth and mushroom weight forA. cylindraceaand with earliness for all species examined. TPOMW-based media revealed a great potential for the substitution of traditional cultivation substrates by valorizing environmentally hazardous agricultural waste.


Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
K Vougogiannopoulou ◽  
H Pratsinis ◽  
R Grougnet ◽  
M Halabalaki ◽  
D Kletsas ◽  
...  

2019 ◽  
Vol 18 (6) ◽  
pp. 1297-1309
Author(s):  
Antonis A. Zorpas ◽  
Maria K. Doula ◽  
Vassilis J. Inglezakis ◽  
Jose Pedreno Navvaro ◽  
Dimitrios J. Bilalis

2021 ◽  
pp. 1-17
Author(s):  
Anna Assimakopoulou ◽  
Ioannis Salmas ◽  
Aikaterina Tsikra ◽  
Alexandros-Iasonas Bastas ◽  
Maria Bakea ◽  
...  

2013 ◽  
Vol 48 (9) ◽  
pp. 767-775 ◽  
Author(s):  
David Peña ◽  
Ángel Albarrán ◽  
Antonio López-Piñeiro ◽  
José Manuel Rato-Nunes ◽  
Javier Sánchez-Llerena ◽  
...  

2017 ◽  
Vol 54 ◽  
pp. 128-134 ◽  
Author(s):  
Virginia Borroni ◽  
Maria Teresa González ◽  
Amalia Antonia Carelli

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