The Turmell-Metre: Using Draw Wire and Inertial Sensors for an In Vivo Ankle Kinematics Study

2021 ◽  
Author(s):  
Julio Hernando Vargas Riaño ◽  
Ángel Valera ◽  
Óscar Agudelo
Keyword(s):  
Inertial Sensors ◽  
Ankle Kinematics ◽  
Ankle Anatomy ◽  
3D Printed

This work presents a device for the ankle kinematics study. It is portable, affordable and some parts can be 3D printed. It is based on the ankle anatomy and biomechanics, also, it can be applied for the characterization of the ankle axis or another models of the ankle. <br>

scholarly journals The Turmell-Metre: Using Draw Wire and Inertial Sensors for an In Vivo Ankle Kinematics Study

2021 ◽  
Author(s):  
Julio Hernando Vargas Riaño ◽  
Ángel Valera ◽  
Óscar Agudelo
Keyword(s):  
Inertial Sensors ◽  
Ankle Kinematics ◽  
Ankle Anatomy ◽  
3D Printed

This work presents a device for the ankle kinematics study. It is portable, affordable and some parts can be 3D printed. It is based on the ankle anatomy and biomechanics, also, it can be applied for the characterization of the ankle axis or another models of the ankle. <br>

scholarly journals Fabrication and Characterization of Ta–GelMA–BG Scaffolds by Chemical Crosslinking Processing for Promotion Osteointegration

2021 ◽  
Vol 8 ◽  
Author(s):  
Fujian Zhao ◽  
Xiongfa Ji ◽  
Yang Yan ◽  
Zhen Yang ◽  
Xiaofeng Chen ◽  
...  
Keyword(s):  
Early Stage ◽  
Bone Defects ◽  
Chemical Crosslinking ◽  
Composite Scaffolds ◽  
Load Bearing ◽  
3D Printed ◽  
Fabrication And Characterization ◽  
Cell Adhesion And Proliferation

The repair of bone defects in load-bearing positions still faces great challenges. Tantalum (Ta) has attempted to repair bone defects based on the excellent mechanical properties. However, the osseointegration of Ta needs to be improved due to the lack of osteoinduction. Herein, tantalum–gelatin–methacryloyl–bioactive glass (Ta–GelMA–BG) scaffolds were successfully fabricated by loading BG in 3D-printed Ta scaffolds through a chemical crosslinking method. The results showed that the composite scaffolds have the ability to promote cell adhesion and proliferation. The incorporation of BG resulted in a significant increase in apatite-forming and osteogenesis differentiation abilities. In vivo results indicated that the Ta–GelMA–BG scaffolds significantly enhanced the osteointegration at the early stage after implantation. Overall, the Ta–GelMA–BG scaffolds are a promising platform for the load bearing bone regeneration field.

scholarly journals The Turmell-Meter: In Vivo Ankle Kinematics by Using Draw-Wire and Inertial Sensors

2021 ◽  
Author(s):  
Julio Hernando Vargas Riaño
Keyword(s):  
Range Of Motion ◽  
Inertial Sensors ◽  
Screw Theory ◽  
Theory Model ◽  
Battery Management ◽  
Ankle Motion ◽  
Novel Device ◽  
Ankle Kinematics ◽  
Measurement Units

Objective: To implement a prototype specific for human ankle kinematics studies in limited spaces, immobile, or lying down patients. Based on anatomy and anthropometry, using a screw theory model, draw-wire and inertial sensors were employed Methods: We included ankle injury studies to highlight the importance of measuring the in vivo range of motion; we studied the ankle anatomy, biomechanics, and anthropometry to estimate the size and movements of the device. We simulated the biaxial representation of ankle motion through the product of exponential mapping. Finally, we designed a structure based on trilateration by projecting tetrahedrons, an acquisition circuit with firmware and calibration software. Results: The prototype has two main parts: support and adjustable platform. We proposed a method to find the position by projecting three apexes on the base using draw-wire sensors, an acquisition board, a single-board computer, a display, Bluetooth, Wi-Fi, and two inertial measurement units. The power source had battery backup with boost and buck converters. Conclusion: We proposed an ankle model in the screw theory framework, a method for localization, and a novel device for in vivo measurements specific for lying patients on a bed, the ground, outdoors, or remote locations without complex setups. The double-battery management is robust and long lasting. Significance: The device is an alternative for measuring the range of motion in laying down patients. We will use it in modeling, diagnosis, and rehabilitation.<br>

scholarly journals The Turmell-Meter: In Vivo Ankle Kinematics by Using Draw-Wire and Inertial Sensors

2021 ◽  
Author(s):  
Julio Hernando Vargas Riaño
Keyword(s):  
Range Of Motion ◽  
Inertial Sensors ◽  
Screw Theory ◽  
Theory Model ◽  
Battery Management ◽  
Ankle Motion ◽  
Novel Device ◽  
Ankle Kinematics ◽  
Measurement Units

Objective: To implement a prototype specific for human ankle kinematics studies in limited spaces, immobile, or lying down patients. Based on anatomy and anthropometry, using a screw theory model, draw-wire and inertial sensors were employed Methods: We included ankle injury studies to highlight the importance of measuring the in vivo range of motion; we studied the ankle anatomy, biomechanics, and anthropometry to estimate the size and movements of the device. We simulated the biaxial representation of ankle motion through the product of exponential mapping. Finally, we designed a structure based on trilateration by projecting tetrahedrons, an acquisition circuit with firmware and calibration software. Results: The prototype has two main parts: support and adjustable platform. We proposed a method to find the position by projecting three apexes on the base using draw-wire sensors, an acquisition board, a single-board computer, a display, Bluetooth, Wi-Fi, and two inertial measurement units. The power source had battery backup with boost and buck converters. Conclusion: We proposed an ankle model in the screw theory framework, a method for localization, and a novel device for in vivo measurements specific for lying patients on a bed, the ground, outdoors, or remote locations without complex setups. The double-battery management is robust and long lasting. Significance: The device is an alternative for measuring the range of motion in laying down patients. We will use it in modeling, diagnosis, and rehabilitation.<br>

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

Comparison of Immunological and Functional Assays for Measurement of Soluble Fibrin

1995 ◽  
Vol 74 (02) ◽  
pp. 673-679 ◽  
Author(s):  
C E Dempfle ◽  
S A Pfitzner ◽  
M Dollman ◽  
K Huck ◽  
G Stehle ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Low Grade ◽  
Plasma Samples ◽  
Normal Range ◽  
Soluble Fibrin ◽  
Discriminating Power ◽  
Fibrin Monomer ◽  
Cerebral Ischemic

SummaryVarious assays have been developed for quantitation of soluble fibrin or fibrin monomer in clinical plasma samples, since this parameter directly reflects in vivo thrombin action on fibrinogen. Using plasma samples from healthy blood donors, patients with cerebral ischemic insult, patients with septicemia, and patients with venous thrombosis, we compared two immunologic tests using monoclonal antibodies against fibrin-specific neo-epitopes, and two functional tests based on the cofactor activity of soluble fibrin complexes in tPA-induced plasminogen activation. Test A (Enzymun®-Test FM) showed the best discriminating power among normal range and pathological samples. Test B (Fibrinostika® soluble fibrin) clearly separated normal range from pathological samples, but failed to discriminate among samples from patients with low grade coagulation activation in septicemia, and massive activation in venous thrombosis. Functional test C (Fibrin monomer test Behring) displayed good discriminating power between normal and pathological range samples, and correlated with test A (r = 0.61), whereas assay D (Coa-Set® Fibrin monomer) showed little discriminating power at values below 10 μg/ml and little correlation with other assays. Standardization of assays will require further characterization of analytes detected.

Insulinomimetic properties of trace elements and characterization of their in vivo mode of action

Diabetes ◽  
1990 ◽  
Vol 39 (10) ◽  
pp. 1243-1250 ◽  
Author(s):  
L. Rossetti ◽  
A. Giaccari ◽  
E. Klein-Robbenhaar ◽  
L. R. Vogel
Keyword(s):  
Trace Elements ◽  
Mode Of Action

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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