COMPARISON OF FOURTH GENERATION ELISA AND RAPID DIAGNOSTIC TEST FOR DIAGNOSIS OF HEPATITIS C VIRUS(HCV) INFECTION IN A TERTIARY CARE HOSPITAL.

2021 ◽  
pp. 15-17
Author(s):  
Stuti Kansra Arora ◽  
Mala Chhabra ◽  
Anuradha Anuradha ◽  
Arvind Achra ◽  
Nandini Duggal
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Treatment Initiation ◽  
Tertiary Care ◽  
Hcv Infection ◽  
Fourth Generation ◽  
World Population ◽  
Test Results ◽  
Blood Samples

Introduction:Hepatitis C virus (HCV) infection has emerged as one of the major global health challenge affecting about 2 - 3% of the world population. Epidemiological studies have shown that HCV infection is a major risk factor for development of Acute hepatitis,chronic liver disease,cirrhosis and Hepatocellular carcinoma (HCC).Early diagnosis of HCV is important to link hepatitis testing to care and treatment initiation. Aim:To compare sensitivity and specificity of rapid diagnostic test (RDT) with fourth generation ELISA Material and Method: This study was conducted in the Department of Microbiology at Atal Bihari Vajpayee Institute of Medical Sciences (formerly Post Graduate Institute of Medical Education and Research) and Dr Ram Manohar Lohia Hospital from January 2018 to December 2018.Blood samples of patients suspected with hepatitis were tested using ELISA and rapid diagnostic test Results: In our study 26378 blood samples were tested for HCV,using fourth generation ELISA.Of these,581(2.20%) samples were found to be positive by ELISA.These HCV positive samples along with equal number of ELISA negative samples were tested by rapid diagnostic test. Sensitivity and specificity of the rapid diagnostic test was found to be 72.98 % and 100% respectively. Discussion:Rapid diagnostic test can be used during emergency hours but their results must be followed by ELISA test results in a tertiary care hospital.Reporting of false negative results should be minimized for rapid linkage to treatment initiation and to avoid silent transmission of infection.

scholarly journals SMS messages increase adherence to rapid diagnostic test results among malaria patients: results from a pilot study in Nigeria

2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Sepideh Modrek ◽  
Eric Schatzkin ◽  
Anna De La Cruz ◽  
Chinwoke Isiguzo ◽  
Ernest Nwokolo ◽  
...  
Keyword(s):  
Pilot Study ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Test Results

scholarly journals Causes of false-positive HIV rapid diagnostic test results

2013 ◽  
Vol 12 (1) ◽  
pp. 49-62 ◽  
Author(s):  
Derryck Klarkowski ◽  
Daniel P O’Brien ◽  
Leslie Shanks ◽  
Kasha P Singh
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
False Positive ◽  
Test Results

scholarly journals Adherence to malaria rapid diagnostic test results and a qualitative study on Health Workers perception about the test in Agona East District of Ghana

2020 ◽  
Author(s):  
Selina Ansah-Koi
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Health Workers ◽  
Health Planning ◽  
National Level ◽  
Health Facilities ◽  
Health Centers ◽  
Quantitative Aspect ◽  
Test Results ◽  
Negative Test

Abstract Background Ghana has adopted the policy of diagnostically testing for malaria before treatment. The Rapid Diagnostic Test (RDT) is used to diagnose malaria especially in lower health facilities like Health Centers (HC) and Community-based Health Planning Services (CHPS) Compounds without microscopy. Studies have shown that clinicians do not always adhere to negative malaria test results of the RDT and prescribe anti-malarials. Methods Quantitative aspect focused on how malaria is diagnosed and adherence to negative rapid diagnostic test results using the consulting room register and patients’ folder of six health facilities within the period of July to September 2015. In-depth interviews using purposive sampling was conducted for 14 health workers at Health Centers and CHPS compounds to find out their perceptions about malaria RDT and reasons for adherence or non-adherence to test results.Results Of the six health facilities in the Agona East District, 3.87% (14/362) were presumptively diagnosed with malaria whiles 96.10% (348/362) were diagnosed with malaria using RDT.For those tested with RDT, 15.25% tested negative and 84.75% tested positive. Of those who tested negative, 20.75% were given anti-malarials and 79.25% did not receive anti-malarials. Health workers perceived the Malaria RDT kit as easy to use and also as making work easier. Testing is done mainly because it is a policy. Conclusion The malaria testing rate in the Agona East District is higher than that at the National level. Health Centers compared to CHPS compounds are likely to treat for malaria in cases of a negative test result. There is also high adherence to negative test results. Health workers perceive the RDT as a useful tool which has made malaria diagnosis easy.

scholarly journals 776. The prevalence of HIV among hospitalized persons with acute febrile illness in rural Uganda, August 2019-June 2020

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S432-S432
Author(s):  
Paul W Blair ◽  
Kenneth Kobba ◽  
Matthew Robinson ◽  
Emmanuel Candia ◽  
Gilbert Aniku ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Hepatitis A ◽  
Febrile Illness ◽  
Blood Cultures ◽  
Fourth Generation ◽  
Newly Diagnosed ◽  
Cryptococcal Antigen ◽  
Referral Hospitals

Abstract Background In Uganda, in the early PEPFAR era, HIV coinfections were responsible for most hospitalizations with febrile illness (as high as 85% in 2006). Currently, national guidelines recommend universal antiretroviral therapy ideally before the development of AIDS. We evaluated the prevalence of HIV among patients admitted to two regional referral hospitals with febrile illness in the era of ‘Treat All.’ Methods Participants admitted to two regional referral hospitals in Uganda were enrolled at emergency departments or medical wards. Participants uniformly received blood cultures, malaria (rapid diagnostic test), and tuberculosis (Xpert MTB/RIF Ultra), hepatitis A IgM, hepatitis B sAg, and HIV fourth generation testing were performed. Among participants with HIV, cryptococcal antigen testing and urine lipoarabinomannan (LAM) were performed. Results From August 2019-June 2020, 95 participants (58% female) with an average age of 36.2 (SD 14.1) years, presented from 11 districts in Uganda. Participants presented at facilities 4.9 days (SD: 2.5) after onset of symptoms. Additionally, 16.1% of participants had a qSOFA (quick Sepsis Related Organ Failure Assessment) severity score of 2 or greater. By 28 days, 11.0% (n=7) died and 10.8% were lost to follow-up. On admission, 25.3% (n=24) of participants had a known history of HIV, the majority (87.5%) were on ART on hospital presentation. Of the 9.5% (n=9) who were newly diagnosed with HIV during the admission 6 were started on ART during hospitalization or within a month after hospitalization. Microbiologic and rapid diagnostic test results included positive results for tuberculosis (2.1%, 2/95 PCR; 3/16 urine LAM), malaria (29.5%, 28/95), cryptococcal antigen positive (12.5%; 2/15), hepatitis A (1.1%, 1/95), and hepatitis B (5.3%, 5/95). Blood cultures were positive in 11.1% of patients (10/90) with S. pneumoniae being most common isolate (N=4). Conclusion In the universal ART era, the proportion of hospitalized febrile patients with HIV has decreased. Overall, 10% have newly diagnosed infection emphasizing the importance of continuing to test all hospitalized febrile patients. Diagnostic evaluations are needed to assess the burden of other causes of febrile illness in order to reprioritize potential differential diagnoses. Disclosures All Authors: No reported disclosures

scholarly journals Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease

2021 ◽  
Vol 11 ◽  
Author(s):  
Aroa Silgado ◽  
Lídia Gual-Gonzalez ◽  
Adrián Sánchez-Montalvá ◽  
Inés Oliveira-Souto ◽  
Lidia Goterris ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Chagas Disease ◽  
Latin American ◽  
Whole Blood ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lower Sensitivity ◽  
Blood Samples ◽  
Screening Programs ◽  
Chronic Chagas Disease

BackgroundChagas disease is a public health problem not only in Latin America, but also in other regions, including Spain, due to migration movements. Conventional serological diagnosis requires an invasive sample (plasma or serum) and a well-equipped laboratory. To circumvent those limitations, blood samples dried on filter paper (DBS) or Rapid Diagnostic Test (RDT) could be a practical alternative to reference protocol for serological screening in epidemiological studies. We evaluated the usefulness of dried blood sampling and a rapid diagnostic test (Trypanosoma Detect™) for the detection of antibodies against T. cruzi for their use in community-based screening.Methodology/Principal FindingsA total of 162 stored paired whole-blood and serum samples from Latin American migrants and 25 negative-control blood samples were included. Diagnosis of chronic Chagas disease was performed in serum according to WHO algorithms. Blood samples were retrospectively collected as dried spots and then analyzed using two different serological techniques, enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (E-CLIA). Whole-blood samples were also used to evaluate a rapid diagnostic test based on immunochromatography. A better correlation with conventional serum was observed in dried blood elutes using E-CLIA than ELISA (97% vs. 77% sensitivity, respectively). Both assays reported 100% specificity. The median cut-off index values of E-CLIA for dried blood were significantly lower than those for serum (138.1 vs. 243.3, P<0.05). The Trypanosoma Detect™ test presented a sensitivity and specificity of 89.6% and 100%, respectively.ConclusionsThe detection of antibodies against T. cruzi in dried blood samples shows a higher sensitivity when using E-CLIA compared with ELISA. Trypanosoma Detect™ is easier to use but has a lower sensitivity. Hence, we propose a sequential strategy based on performing the rapid test first, and a negative result will be confirmed by DBS-ECLIA for use in community Chagas disease screening programs.

scholarly journals Is rapid diagnostic test (malaria Pv/Pf Ag card test) reliable in diagnosing malaria

2017 ◽  
Vol 5 (1) ◽  
pp. 92
Author(s):  
Vishruti Gandhi Vishruti Gandhi ◽  
Prasad Muley ◽  
Niyati Parikh ◽  
Hardik Gandhi ◽  
Akash Mehta
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Peripheral Blood Smear ◽  
Peripheral Smear ◽  
Infected Female ◽  
Anopheles Mosquitoes ◽  
Smear Examination

Background: Malaria is a protozoan disease transmitted by the bite of infected female anopheles mosquitoes is one of the most important parasitic diseases of human with transmission in 109 countries, affecting more than one billion people worldwide. This study was planned to compare the gold standard i.e. peripheral blood smear examination and the newer rapid diagnostic test (malaria plasmodium falciparum/ plasmodium vivax antigen card) to know the diagnostic accuracy of Rapid Diagnostic Test (RDT) kits. Methods: All the suspected cases of WHO defined malaria between 1month to 18 years of age were enrolled in the study.Results: Out of 96 clinically suspected cases of malaria 63 were confirmed by peripheral smear. The age range of participants ranged from 4 months to 17 years. On peripheral smear examination, out of 96 clinically suspected cases, 37 (38.5%) cases were positive for P. vivax, 23 (23.9%) were positive for P. falciparum and 3 (3.1%) were positive for both parasites by microscopy. Sensitivity and specificity of RDT for Plasmodium Vivax is 92.5% and 96.4% respectively. Sensitivity and specificity of RDT for Plasmodium Falciparum is 96.2% and 90%.Conclusions: The rational use of RDTs as a complement to microscopy might give substantial health benefits through earlier treatment, reduction in morbidity and mortality and more rationalized approach for choosing anti-malarial drugs, which in terms may prevent drug resistance.

scholarly journals Typhidot IgM as a reliable and rapid diagnostic test for typhoid fever among children in a tertiary care hospital

2020 ◽  
Vol 7 (8) ◽  
pp. 1677
Author(s):  
Mohammad Ashfaque Ansari ◽  
Amit Kumar Thakur ◽  
Atindra Mishra ◽  
Md Jaffer Rain
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Predictive Value ◽  
Tertiary Care ◽  
Public Health Problem ◽  
Antibiotics Resistance ◽  
Major Public Health Problem ◽  
Care Hospital

Background: Typhoid fever still continues to be a major public health problem in Nepal. A clinical spectrum of typhoid varies widely. It causes significant complication as well as mortality. A simple, reliable, affordable and rapid diagnostic test has been a long felt need of the clinicians to prescribe specific medication, adopt prevention of the emergence of antibiotics resistance and overall reduce the disease burden in the community.Methods: The prospective descriptive study was performed in 125 children between 2 years to 15 years of age admitted to the Pediatrics Department from September 2017 to September 2018. Blood culture, Typhidot rapid IgM were performed. MEDCALC software was used to calculate 95% confidence interval for sensitivity, specificity, predictive value positive, predictive value negative and accuracy. Kappa test was used to determine the agreement between Typhidot IgM and blood culture methods.Results: The study consisted of 125 children with acute febrile illness for more than 3 days with clinical symptomatology, consistent with typhoid fever. The reliability of Typhidot IgM in relation with blood culture and the study lighten that sensitivity 92.3% (95% CI: 63.9, 99.8), specificity 49.1% (95% CI: 39.5, 58.7), PPV 17.4% (95% CI: 14.2, 21.1), NPV 98.2% (95% CI: 89.2, 99.7) and accuracy 53.6% (95% CI: 44.5, 62.6). The two methods i.e. Typhoid IgM and blood culture shows significant agreement with p value 0.004.Conclusions: The present study demonstrates that Typhidot IgM has all the attributes of an ideal screening test.

scholarly journals Sensitivity and specificity of a rapid test for assessment of exposure to SARS-CoV-2 in a community-based setting in Brazil

2020 ◽  
Author(s):  
Lucia Campos Pellanda ◽  
Eliana Márcia da Ros Wendland ◽  
Alan John Alexander McBride ◽  
Luciana Tovo-Rodrigues ◽  
Marcos Roberto Alves Ferreira ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Rapid Test ◽  
Laboratory Diagnosis ◽  
Epidemiological Studies ◽  
Population Based ◽  
Serum Samples ◽  
Kappa Score ◽  
Qrt Pcr

AbstractBackgroundWhile the recommended laboratory diagnosis of COVID-19 is a molecular based assay, population-based studies to determine the prevalence of COVID-19 usually use serological assays.ObjectiveTo evaluate the sensitivity and specificity of a rapid diagnostic test for COVID-19 compared to quantitative reverse transcription polymerase chain reaction (qRT-PCR).MethodsWe evaluated the sensitivity using a panel of finger prick blood samples from participants >18 years of age that had been tested for COVID-19 by qRT-PCR. For assessing specificity, we used serum samples from the 1982 Pelotas (Brazil) Birth Cohort participants collected in 2012 with no exposure to SARS-CoV-2.ResultsThe sensitivity of the test was 77.1% (95% CI 66.6 - 85.6), based upon 83 subjects who had tested positive for qRT-PCR at least 10 days before the rapid diagnostic test (RDT). Based upon 100 sera samples, specificity was 98.0% (95% CI 92.9 - 99.8). There was substantial agreement (Kappa score 0.76) between the qRT-PCR results and the RDT.InterpretationThe validation results are well in line with previous assessments of the test, and confirm that it is sufficiently precise for epidemiological studies aimed at monitoring levels and trends of the COVID-19 pandemic.

scholarly journals Disease Prevalence Matters: Challenge for SARS-CoV-2 Testing

Antibodies ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 50
Author(s):  
Chin-Shern Lau ◽  
Tar-Choon Aw
Keyword(s):  
Diagnostic Test ◽  
Sensitivity And Specificity ◽  
False Positive ◽  
Disease Prevalence ◽  
Test Results ◽  
Community Screening ◽  
Predictive Values ◽  
Low Prevalence

While sensitivity and specificity are important characteristics for any diagnostic test, the influence of prevalence is equally, if not more, important when such tests are used in community screening. We review the concepts of positive/negative predictive values (PPV/NPV) and how disease prevalence affects false positive/negative rates. In low-prevalence situations, the PPV decreases drastically. We demonstrate how using two tests in an orthogonal fashion can be especially beneficial in low-prevalence settings and greatly improve the PPV of the diagnostic test results.

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