scholarly journals Pollen Viability and Germinability of Gamma Irradiated M4 Lines of Sesame

2021 ◽  
Vol 3 (2) ◽  
pp. 11-18
Author(s):  
M. A. I. Audu ◽  
O. A. Falusi ◽  
M. L. Muhammad ◽  
O. A. Y. Daudu ◽  
A. Abubakar
Keyword(s):  
Sucrose Concentration ◽  
Pollen Viability ◽  
Block Design ◽  
Standard Procedure ◽  
Polar View ◽  
River Basin Development ◽  
Breeding Programmes ◽  
Gamma Irradiated ◽  
Pollen Diameter ◽  
Mutant Lines

Pollen viability and germinability are indispensable criteria in plant breeding programmes. This research evaluated eleven M4 mutant lines of gamma irradiated sesame (Sesamum indicum) for some pollen parameters. Seeds were obtained from the Department of Plant Biology, Federal University of Technology, Minna and were raised to maturity alongside their respective checks in a randomized complete block design (RCBD). The field experiment was conducted at the experimental field of the Upper Niger River Basin Development Authority, Minna. Pollen viability and diameter were determined using standard procedure. The germinability test was done using three different sucrose concentrations (10 %, 20 % and 30 %) with 1 % nutrient agar solution. The results revealed that all the M4 mutant lines had adequate pollen viabilities (over 80%) with ML-10 having the highest (97.56 %) viability followed by ML-7 (95.61 %), ML-8 (95.01 %) and Check-2 (95.60 %). The highest pollen germinability was recorded at 20 % sucrose concentration for all the mutant lines with line ML-7 (39.70 %) having the highest percentage. Check-1 at 10 % sucrose concentration recorded the least percentage (11.46 %) across the concentration and treatments. Highest pollen diameter (169.52μm) was recorded in Check-1. Suboblate shapes with 10-13 colpi was observed in all the mutant lines and the checks. Pollens from all the lines comprised of circular and elliptic pollens except in ML-7 and the checks where the pollens were solely circular in polar view. The study revealed that gamma-irradiation could be a reasonable tool for inducing variability in sesame and advantageous in increasing the pollen viability.

scholarly journals Chemical and Biological Profiles of Dendrobium in Two Different Species, Their Hybrid, and Gamma-Irradiated Mutant Lines of the Hybrid Based on LC-QToF MS and Cytotoxicity Analysis

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1376
Author(s):  
Bomi Nam ◽  
Hyun-Jae Jang ◽  
Ah-Reum Han ◽  
Ye-Ram Kim ◽  
Chang-Hyun Jin ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Squamous Carcinoma ◽  
Ultra Performance Liquid Chromatography ◽  
Carcinoma Cells ◽  
Traditional Medicines ◽  
Flight Mass Spectrometry ◽  
Squamous Carcinoma Cells ◽  
Chemical Profiles ◽  
Gamma Irradiated ◽  
Mutant Lines

The Dendrobium species (Orchidaceae) has been cultivated as an ornamental plant as well as used in traditional medicines. In this study, the chemical profiles of Dendrobii Herba, used as herbal medicine, Dendrobium in two different species, their hybrid, and the gamma-irradiated mutant lines of the hybrid, were systematically investigated via ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QToF MS). Among the numerous peaks detected, 17 peaks were unambiguously identified. Gigantol (1), (1R,2R)-1,7-hydroxy-2,8-methoxy-2,3-dihydrophenanthrene-4(1H)-one (2), tristin (3), (−)-syringaresinol (4), lusianthridin (5), 2,7-dihydroxy-phenanthrene-1,4-dione (6), densiflorol B (7), denthyrsinin (8), moscatilin (9), lusianthridin dimer (10), batatasin III (11), ephemeranthol A (12), thunalbene (13), dehydroorchinol (14), dendrobine (15), shihunine (16), and 1,5,7-trimethoxy-2-phenanthrenol (17), were detected in Dendrobii Herba, while 1, 2, and 16 were detected in D. candidum, 1, 11, and 16 in D. nobile, and 1, 2, and 16 in the hybrid, D. nobile × candidum. The methanol extract taken of them was also examined for cytotoxicity against FaDu human hypopharynx squamous carcinoma cells, where Dendrobii Herba showed the greatest cytotoxicity. In the untargeted metabolite analysis of 436 mutant lines of the hybrid, using UPLC-QToF MS and cytotoxicity measurements combined with multivariate analysis, two tentative flavonoids (M1 and M2) were evaluated as key markers among the analyzed metabolites, contributing to the distinction between active and inactive mutant lines.

scholarly journals DIFFERENT STABILITY METHODS FOR CULTIVAR RECOMMENDATION IN ELEPHANT-GRASS FOR ENERGY PURPOSES IN BRAZIL

CERNE ◽  
2017 ◽  
Vol 23 (4) ◽  
pp. 507-515 ◽  
Author(s):  
Veronica Brito da Silva ◽  
Rogerio Figueiredo Daher ◽  
Bruna Rafaela da Silva Menezes ◽  
Maria Lorraine Fonseca Oliveira ◽  
Maria do Socorro Bezerra Araújo ◽  
...  
Keyword(s):  
Energy Production ◽  
Dry Matter ◽  
Block Design ◽  
Dry Matter Yield ◽  
Elephant Grass ◽  
Randomized Block Design ◽  
Matter Production ◽  
Breeding Programmes ◽  
The Stability ◽  
Non Parametric

ABSTRACT The elephant-grass undergoes successive cutting and periodical evaluations that it possible to identify clones with high stability for dry matter production, which can be used for energy production. The present study was carried out to evaluate stability dry matter yield for different parametric and non-parametric methods in elephant grass genotypes for biomass production undergoes successive cutting in the agroclimatic conditions of the Norte Fluminense (RJ, Brazil). The variable measured in the 40 genotypes was dry matter yield (DMY) at 2009, 2010 and 2011 in a field study designed as randomized block design with two replicates. Each sample was grown in different environment condition. The stability methods tested were the Yates and Cochran’s, Plaisted and Peterson’s, Wricke’s ecovalence, Annicchiarico’s, Lin and Binns’ and Kang and Phan’s. Results indicated that cutting (E) and genotypes (G) influenced significantly on the performance of dry matter yield. The non-parametric stability methods were effective for the evaluation of stability in dry matter yield. Genotypes Mercker, Pinda-México, Mercker 86-México, Guaçu/IZ, Mercker Pinda, P-241-Piracicaba and Cubano Pinda were stable stability dry matter yield. Hence, there are genotypes may be exploited in future breeding programmes in order to improve productivity of upland elephant grass over environment.

scholarly journals Effects of a fungicide on the morphology and viability of pollens of tomato (Lycopersicon esculentum Mill.)

1970 ◽  
Vol 38 (2) ◽  
pp. 115-118 ◽  
Author(s):  
Ilkay Öztürk Çali ◽  
Feyza Candan
Keyword(s):  
Lycopersicon Esculentum ◽  
Phytophthora Infestans ◽  
Key Words ◽  
Pollen Morphology ◽  
Pollen Viability ◽  
Polar View ◽  
Control Group ◽  
Recommended Dosage ◽  
Tomato Plants ◽  
Equatorial View

Effects of Agri Fos 400 (Mono and di-potassium phosphanate), a fungicide widely used on tomatoes grown in greenhouses in Turkey against Phytophthora infestans were studied on the morphology and viability of tomato (Lycopersicon esculentum Mill.) pollens. The fungicide was applied on tomato plants at recommended dosage (4 ml/l water) and double the recommended dosage (8 ml/l water). The fungicide caused changes in the morphological structures of pollens. Some pollen morphological structures that are not observed in the control group were encountered in the pollens due to application of Agri Fos 400 in equatorial view at 8 ml/l and in polar view at 4 ml/l. Level of pollen viability decreased as the dosage increased.   Key words: Fungicide; Phytophthora infestans; Tomato; Pollen morphology; Viability DOI: 10.3329/bjb.v38i2.5134 Bangladesh J. Bot. 38(2): 115-118, 2009 (December)

Pollen–pistil interaction, pistil histology and seed production in A×B grain sorghum crosses under chilling field temperatures

2009 ◽  
Vol 148 (1) ◽  
pp. 73-82 ◽  
Author(s):  
M. E. CISNEROS-LÓPEZ ◽  
L. E. MENDOZA-ONOFRE ◽  
H. A. ZAVALETA-MANCERA ◽  
V. A. GONZÁLEZ-HERNÁNDEZ ◽  
G. MORA-AGUILERA ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Seed Production ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Vascular Tissue ◽  
Pollen Viability ◽  
Tube Growth ◽  
Male Sterile ◽  
Pollen Diameter

SUMMARYSix pairs of isogenic lines of sorghum (Sorghum bicolor L. Moench) were sown in field plots in Montecillo, State of México (2240 m altitude), in 2005 and 2006. Crosses A (♀)×B (♂) were done in each pair. In A-lines, the length of pistil, stigma, style and ovary, as well as the ovary width, were measured. In B-lines, pollen diameter, viability (cytoplasm density) and production were evaluated. Pollen germination and pollen tube growth in the pistils of the A-lines, were quantified in vivo with aniline blue and epifluorescence 18 h after pollination (HAP), while fertilized pistils were counted at 96 HAP. Histological studies on both pollinated and non-pollinated pistils were performed in one male-sterile line. Seed yield, mean-seed weight, seeds per panicle and seed set (SS; seeds/flower/panicle) were determined at harvest. Pollen viability was the variable most related to pollen germination and pollen tube growth. Stigma receptivity was not associated with its morphology. Growth of the pollen tube in stigma, style and ovary was observed in the transmitting tissue 18 HAP, running parallel to the vascular tissue. Yield under chilling field temperatures (minimum average of 6 and 8°C) prevailing during flower development and pollination ranged from 7 to 12 g/panicle. The differences in seed production and SS among A×B crosses did not depend on the amount and viability of pollen.

scholarly journals Evaluation of Seed Yield of Nigerian Bambara Groundnut [Vigna subterranea (L.) Verdc.] Landraces under Varying Water Conditions

2018 ◽  
Vol 10 (2) ◽  
pp. 233-239 ◽  
Author(s):  
Dorcas Ropo ABEJIDE ◽  
Olamide A. FALUSI ◽  
Andrew S. GANA ◽  
Matthew O. ADEBOLA ◽  
Oladipupo A.Y. DAUDU ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Yield ◽  
Block Design ◽  
Bambara Groundnut ◽  
High Yield ◽  
Stressed Condition ◽  
Drought Tolerant ◽  
University Of Technology ◽  
Water Conditions ◽  
Breeding Programmes

Seed yield of selected Nigerian Bambara Groundnut landraces under two varying water conditions was evaluated. Twenty eight (28) Bambara groundnut accessions were planted in a randomized complete block design with two treatments (Non-water stressed and Water stressed) and replicated five times. The experiment was conducted at the Experimental Garden of the Department of Biological Sciences, Federal University of Technology, Minna, Niger State. The accessions were planted in planting bags during the cropping season of 2016 between July and October. The two treatments were rain fed until maturity except during the period of exposure to water stress where the water stressed plants were transferred to a screen house made with polyvinyl ceiling to prevent water during the flowering stage for two weeks. It was observed that water stress reduced the yield of the Bambara groundnut landraces by 47%. Accessions NGB-01646-B, NGR-NI-20-H  and NGB-01491 which had relatively high yield in the stressed and non- water stressed condition were considered  the most drought tolerant accessions and accession NGR-KG-02-C with low yield both in stressed and non-stressed conditions was identified as the most susceptible accession. Accessions which had high yield only in water stressed conditions could serve as elite line for Bambara groundnut for areas which have problem of drought. These drought tolerant accessions identified in the research can serve as potential parent lines in breeding programmes aimed at the development of drought tolerant varieties of Bambara groundnut.

scholarly journals Descriptive Statistics and Heritability for Agronomic Traits and Grain Micronutrient Content in Rice (Oryza sativa L.)

2019 ◽  
pp. 1-10
Author(s):  
Avinash Kumar ◽  
Ashutosh Kumar ◽  
N. K. Singh ◽  
Rajesh Kumar ◽  
S. K. Singh ◽  
...  
Keyword(s):  
Grain Yield ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Block Design ◽  
Zinc Content ◽  
Yield Potential ◽  
High Yield ◽  
Grain Zinc ◽  
Iron And Zinc ◽  
Breeding Programmes

In the present investigation, 10 parents and their 45 crosses (half diallel) along with 2 standard checks (Rajendra Nilam and Rajendra Mahsuri-1) were evaluated during Kharif, 2018 in Randomized Complete Block Design (RCBD) with 3 replications at Rice Farm Section, Dr. Rajendra Prasad Central Agricultural University (RPCAU), Pusa, Bihar. The objective of this study was to identify promising rice genotypes having desirable combination of morphological traits along with high grain iron and zinc content and high grain yield potential. The results of variability parameters indicated that ample amount of genetic variability was present for all the studied traits. Most of the traits showed high heritability coupled with high genetic advance indicating fruitfulness of selection for improvement of these traits. One genotype (P2×P7) with high grain iron (16.10 ppm) and grain zinc (26.40 ppm) content along with high yield (43.12 g/plant) was identified. Genotypes with high grain iron coupled with high grain yield (P7×P9, P8×P9, P5×P7 and P5×P9) and high  grain zinc content coupled with high grain yield (P4×P7, P9×P10, P8×P9 and P5×P7) were also identified. These promising genotypes identified can be used further in breeding programmes to obtain superior segregants with high grain micronutrient content and high grain yield.

scholarly journals Genotype by Environment Interaction for Grain Yield of Barley Mutant Lines

2019 ◽  
Vol 65 (2) ◽  
pp. 51-58
Author(s):  
Boryana Dyulgerova ◽  
Nikolay Dyulgerov
Keyword(s):  
Grain Yield ◽  
Block Design ◽  
Genotype By Environment Interaction ◽  
Winter Barley ◽  
Environment Interaction ◽  
Genotype By Environment ◽  
Growing Seasons ◽  
Stable Mutant ◽  
Check Variety ◽  
Mutant Lines

Abstract The aim of this study was to examine the genotype by environment interaction for grain yield and to identify high-yielding and stable mutant lines of 6-rowed winter barley under different growing seasons. The study was carried out during 7 growing seasons from 2010 – 2011 to 2016 – 2017 in the experimental field of the Institute of Agriculture – Karnobat, Southeastern Bulgaria. Fourteen advanced mutant lines and the check variety Vesletc were studied using a complete block design with 4 replications. The AMMI analysis of variance indicated that 20.54% of the variation for grain yield was explained by the effect of genotype and 37.34% and 42.12% were attributable to the environmental effects and genotype by environment interaction. The magnitude of the genotype by environment interaction was two times larger than that of genotypes, indicating that there was a substantial difference in genotype response across environments. The AMMI and GGE biplot analyses identified G9 as the highest yielding and stable genotype. This mutant line can be recommended for further evaluation for variety release. The mutant lines G6, G13 and G15 were suggested for inclusion in the breeding program of winter barley due to its high grain yield and intermediate stability.

scholarly journals Comparative Growth Performance of Papaya Hybrids and their Parents under Reciprocal Crossing System

2020 ◽  
pp. 167-173
Author(s):  
Suchismita Jena ◽  
T. R. Ahlawat ◽  
A. I. Patel ◽  
A. K. Pandey ◽  
Dharmishtha Patel ◽  
...  
Keyword(s):  
Vegetative Growth ◽  
Growth Parameters ◽  
Block Design ◽  
Germination Percentage ◽  
Internodal Length ◽  
Randomized Block Design ◽  
Growth Performances ◽  
Breeding Programmes ◽  
Number Of Leaves ◽  
Reciprocal Crossing

Papaya is a very popular fruit in the tropical and subtropical regions of the world and has witnessed an increase in demand. This has fuelled an interest to develop region specific hybrids with desirable vegetative growth and vigour.  Hence a field experiment was carried out at College of horticulture and forestry, Navsari Agricultural University in Gujarat during 2018-2020 in randomized block design with three replications to know the mean performance of parents and hybrids for comparative growth performances of papaya. Wherein, thirty papaya hybrids were developed from six morphologically distinct parents through full diallel crossing technique and all the parents and hybrids were evaluated for their germination and growth parameters. The variability was distinct in all hybrids and parents for germination percentage, days taken to germination, periodical plant height and girth, number of leaves, petiole length and internodal distance. Amongst all the parents, Pusa Delicious, CO-8 and Lucknow Local exhibited improved germination, better vegetative growth and dwarf stature, respectively. Among the hybrids, Pusa Delicious x Lucknow Local was found to have dwarf stature with minimum internodal length however, the reciprocal cross: Lucknow Local x Pusa Delicious recorded maximum plant girth and number of leaves. Based on their mean performance, these parents can be used for further breeding programmes and hybrids could be exploited for cultivation.

scholarly journals Evaluation of M3 lines of sesame (Sesamum indicum L.) for vegetative parameters

2021 ◽  
Vol 13 (2) ◽  
pp. 10795
Author(s):  
Mohammed BABAGANA ◽  
Olamide A. FALUSI ◽  
Aliyu N. ABDULKADIR ◽  
Alhassan MOHAMMED ◽  
Hauwa SHEHU ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Block Design ◽  
Sesamum Indicum ◽  
Petiole Length ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Breeding Programmes ◽  
Number Of Leaves ◽  
Different Doses ◽  
Number Of Branches

Vegetative parameters of eight M3 (third mutant generation) lines of sesame were evaluated under rain fed condition. The seeds of the lines were sown alongside their respective controls using a randomized complete block design. The eight lines were generated from three parental stocks: NCRIBEN-04E (V1), NCRIBEN-01M (V2) and NCRIBEN-03L (V3). The three parental stocks were exposed to different doses of gamma irradiation from cobalt-60 source. 04E-550-G2-3 had the highest plant height (57.40) at the 6th week which was significantly different (P<0.05) from the three checks. 03L-450-G2-2 had the highest petiole length (12.45cm) which was significantly different (P<0.05) from the checks except check three (12.25cm) which was not significantly different (P>0.05). 03L-450-G1-2 had the highest number of leaves (91) and consequently highest number of branches (5.60). Although the number of leaves were significantly different (P<0.05) from Check three (54.60), there was no significant difference (P>0.05) between the number of branches and check three (5.40). The results obtained for vegetative parameters of M3 lines of sesame suggests that some of the genotypes could be used as potential parents for future breeding programmes aimed at improving sesame lines.

scholarly journals Character Association and Path Analysis for Yield Components and Biochemical Traits in Maize (Zea mays L.) Genotypes

2021 ◽  
Author(s):  
Prabhat Kumar Singh ◽  
B. Sushma ◽  
Nidhi Kumari
Keyword(s):  
Grain Yield ◽  
Block Design ◽  
Standard Procedure ◽  
Grain Weight ◽  
Direct Selection ◽  
Path Coefficient ◽  
Biochemical Traits ◽  
Internodal Length ◽  
Selection For ◽  
Positive Direct

Background: Grain yield is a complex character so direct selection for yield as such can be misleading. Keeping in view, the present study aimed to assess the path coefficient analysis with correlation coefficient technique to establish the direct and indirect relationship between the yield and its component. Methods: The present investigation carried out during kharif 2019 at Centurion University of Technology and Management, Odisha in randomized complete block design with 3 replication involving 20 maize genotypes. Data were collected from five randomly selected plnts on 20 agromorphic and biochemical traits. All the biochemical analysis estimated from leaf sample following standard procedure. Result: The traits shelled grain weight, cob diameter, number of grains per cob and anthesis silking interval were found to possess significant association in disirable direction with five ear weight at both genotypic and phenotypic levels. None of the biochemical trairts establishing significant positive association with yield. Highest positive direct effect on 5 ear weight was noticed by shelled grain weight (1.108) followed by phenols (0.115), internodal length (0.106) and days to 50 percent anthesis (0.102) indicating that the selection for these characters were likely to bring about an overall improvement in grain yield directly.

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