scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN KRIM EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 349
Author(s):  
Eklesia Pogaga ◽  
Paulina V. Y. Yamlean ◽  
Julianri S. Lebang
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Traditional Medicine ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Mulberry Leaves ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Ethanol Extracts

ABSTRACTMulberry is one of the plants growing in Indonesian and widely used as traditional medicine. The active compounds found in Mulberry leaves are known have an antioxidant activity. The aim of this study is to formulate cream preparations from ethanol extracts of Mulberry leaves and to determine its antioxidant activities. Mulberry leaves was macerated using ethanol 96%. Antioxidant activity of mulberry leaves cream then evaluated against DPPH as free radical and measured at 517 nm wavelength using a UV-Vis spectrophotometer. The results showed that the cream of ethanol extract from Mulberry leaves had a very strong antioxidant activity with IC50 values in a row that is 1.7831 ppm, 0.8215 ppm and 0.7668 ppm. This can be compared with a Vitamin C solution, which has a very strong antioxidant activity with IC50 value was 1.1113 ppm. This study concludes that the cream from Mulberry leaves ethanol extract has a very strong antioxidant activity.Keywords: Mulberry leaves, Cream, Antioxidant, DPPHABSTRAK Murbei merupakan salah satu tanaman yang tumbuh di Indonesia dan  banyak digunakan dalam pengobatan secara tradisional. Kandungan senyawa aktif yang terdapat pada daun Murbei dikenal mempunyai peranan sebagai antioksidan. Tujuan dari penelitian ini ialah untuk membuat sediaan krim dari ekstrak etanol daun Murbei dan untuk menguji apakah adanya aktivitas antioksidan. Daun murbei diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Sediaan krim diuji terhadap DPPH sebagai radikal bebas dan diukur pada panjang gelombang 517 nm  dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa krim ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat  dengan  nilai IC50 berturut-turut yaitu 1,7831 ppm, 0,8215 ppm dan 0,7668 ppm. Hal ini dapat dibandingkan dengan larutan Vitamin C yang mempunyai aktivitas antioksidan yang sangat kuat, yaitu 1.1113 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa krim dari ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Daun Murbei, Krim, Antioksidan, DPPH

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

scholarly journals Phytochemistry and Antioxidant Activities of the Methanolic Leaf Extract of Clerodendrum splendens (Lamiaceae)

2018 ◽  
pp. 12-20
Author(s):  
Nganso Ditchou Yves Oscar ◽  
Amang A Ngoung GA ◽  
Soh Desire ◽  
Simo Nemg Fredy Brice ◽  
Nyasse Barthelemy
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Maximum Activity ◽  
Hepatic Lipids ◽  
Ic50 Value ◽  
Ic50 Values

This paper aimed at studying the antioxidant efficacy of the methanolic leaf extract of Clerodendrum splendens, a plant of the Lamiaceae family. Phytochemical tests carried out on extracts of Clerodendrum splendens leaves have been able to detect the presence of secondary metabolites such as Flavonoids, Tannins, Saponins and Terpenoids. The results of the antioxidant activity have shown that CSF2, CSF3 fractions and CSB, CSG fractions similarly inhibited hepatic lipids but significantly less than vitamin C. Compared to all fractions, the CSB fraction shows the best inhibitor on the peroxidation of hepatic lipids because at 150 μg/mL, there is a maximum activity (2.5 μg/mL of protein). However, it is found that CSF3, CSF2 and CSG have higher IC50 values than vitamin C (5.613±0.117) while CSEB, CSB and CSC fractions showed lower IC50 values than vitamin C, which is used as the reference reducing compound. The lower the IC50 value compared to vitamin C, the greater the antioxidant capacity of the plant extract. The results of this study suggest that Clerodendrum splendens represents an untapped source of compounds with potential antioxidant activity that could be explored in the development of new therapeutic natural products.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used as well as UV-Vis Spectrophotometry was used to measure vitamin C as a positive control measurement. The samples mixed with ethanol in various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619 mg/L, 16.575 mg/L, 14.729 mg/L, and 16.782 mg/L, respectively. Vitamin C as the positive control has IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BUAH BUNI (Antidesma bunius (L.) SPRENG)

2016 ◽  
Vol 3 (2) ◽  
pp. 159-163
Author(s):  
Arif Rahman ◽  
Abd Malik ◽  
Aktsar Roskiana Ahmad
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Free Radical Scavengers ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dried Fruit ◽  
Ethanol Extracts

Free radicals play a role in the occurrence of various degenerative diseases that require free-radical scavengers or antioxidants. Buni fruit (Antidesma bunius (L.) Spreng) has the bioactive components are Anthocyanins (flavonoids) that serves to the free radicals. This study aimed to measure the antioxidant activity of theethanol extract contained 70% fruit Buni obtained by using the method of nitric oxide. Simplicia buni macerated dried fruit with 70% ethanol. Extracts were obtained in the test antioxidant activity against nitric oxide radicals. The antioxidant activity against free radical absorbance measured by means of UV-Vis spectrophotometry at a wavelength of 546 nm and calcul ated IC50 values. The results shows that the fruit buni has potential as a free radical with IC50 value of 2.28 µg/mL and a comparison of quercetin with IC50 value of 5.88 µg/mL.

scholarly journals Antioxidant Activities of n-Hexane Soluble and Insoluble Fraction, Ethyl Acetate Soluble and Insoluble Fraction from Ethanol Extract of Sambung Nyawa Leaf (Gynura procumbens (Lour.) Merr.)

2019 ◽  
Vol 24 (2) ◽  
pp. 91
Author(s):  
Purwantiningsih Purwantiningsih ◽  
Retno Murwanti ◽  
Lukman Hakim
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Soluble Fraction ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Insoluble Fraction ◽  
Ic50 Value ◽  
Antioxidant Potency

There were many plants containing flavonoids which can function as antioxidant. Antioxidant can be used to decrease the mortality rate caused by degenerative and infectious diseases. This study aims to determine the antioxidant potency of n-hexane soluble and insoluble fraction, ethyl acetate soluble and insoluble fraction, and the ethanol extract antioxidant activity of Sambung Nyawa leaf (Gynura procumbens (Lour.) Merr.). The ethanol extract of G. procumbens leaves was obtained by maceration using 70% ethanol then fractionated using hexane and ethyl acetate to obtain the n-hexane soluble fraction, n-hexane insoluble fraction, ethyl acetate soluble fraction, and ethyl acetate insoluble fraction. The antioxidant activity study of ethanol extract, n-hexane soluble, and insoluble fraction, ethyl acetate soluble, and insoluble fraction of G. procumbens was carried out using DPPH (2,2- diphenyl-1-picrylhydrazyl) method to evaluate its free radical scavenging activity with a comparison of vitamin C. The antioxidants activity was expressed as IC50 value. The IC50 value for vitamin C and ethanol extract of G. procumbens leaves were 2.41 µg/ml and 200 µg/ml. On the other hand, the n-hexane soluble and insoluble fraction had an IC50 value of 78 µg/ml and 34 µg/ml, while ethyl acetate soluble and insoluble fraction had the potency as an antioxidant with an IC50 value of 419 µg/ml and 151 μg/ml, respectively. Based on the results obtained, the antioxidant potency of G. procumbens ethanol extract and their fraction were successively of n-hexane insoluble fraction > n-hexane soluble fraction > ethyl acetate insoluble fraction > ethanol extract > ethyl acetate soluble fraction.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Semangka (Citrullus lanatus)

2018 ◽  
Vol 7 (3) ◽  
pp. 107
Author(s):  
Sri Mariani ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Antioxidant Activities ◽  
Citrullus Lanatus ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Control Measurement ◽  
Ic50 Value ◽  
Ic50 Values

The aim of this study is to determine the IC50 extracts values of white peel and flesh of red watermelon, white peel and flesh of yellow watermelon as antioxidants. To determine antioxidant activities DPPH reagent was used and UV-Vis spectrophotometry was used to measure Vitamin C as a positive control measurement. The samples mixed with ethanol in a various concentration of 5, 15, 25 and 35 mg/L. The results showed that the IC50 values for each extract of red watermelon flesh, yellow watermelon flesh, white peel of red watermelon, and white peel of yellow watermelon were 16.619, 16.575, 14.729, and 16.782 mg/L, respectively. Vitamin C as positive control has an IC50 value of 9.526 mg/L. These IC50 values showed that vitamin C had higher antioxidant activity than watermelon extracts, but the extracts of watermelon still categorized in strong natural antioxidants. This study concluded that watermelon is good to be consumed because it is a very strong antioxidant.

scholarly journals Uji Aktivitas Antioksidan Ekstrak etanol Daun Kratom (Mitragyna Speciosa) Dengan Metode 1, 1 Difenil-2-Pikrihidrazil (DPPH)

2020 ◽  
pp. 204
Author(s):  
Herni Setyawati
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Mitragyna Speciosa ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Herbal Plants ◽  
Layer Chromatography ◽  
Positive Results

ABSTRACT Kratom (Mitragyna speciosa) is one of the herbal plants from Indonesia. Kratom plants have several benefits overcoming diarrhea, increase endurance, and deal with pain. Kratom leaf has several pharmacological effects, one of which is antioxidant. This study aims to determine the antioxidant activity of kratom leaf ethanol extract using the 1.1-Diphenyl-2-Pikrilhidrazil (DPPH) method expressed by IC50 values. Kratom leaves were extracted by the 3x24 hour remaceration method using 70% ethanol solvent, then concentrated with a rotary evaporator at 40 ° C, followed by an oven at a temperature (40-50) ° C to obtain a thick extract. Kratom leaf extract contains alkaloid compounds, flavonoids, tannins,and saponins. The antioxidant activity test was carried out with qualitative and quantitative tests.The qualitative antioxidant test was carried out using the Thin Layer Chromatography (TLC) method with n-hexane: ethyl acetate (7: 3) eluent, followed by DPPH spraying,which showed positive results giving antioxidant activity. The quantitative test for antioxidants was carried out using the 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) method using a  SpectrophotometerUV-Vis with vitamin C as a comparison. The quantitative antioxidant test was carried out using the 1,1-Diphenyl-2-Pikrilhidrazil (DPPH) method with vitamin C as a comparison. The results of antioxidant activity testing of kratom leaf ethanol extract showed an IC50 value of 91.86 ppm,so that it was included as a powerful antioxidant group. Keywords:Antioxidant,DPPH,Ethanol, IC50,Mitragyna speciosa.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals In-Vitro Study of Antioxidant Activities from Ethanol Extracts of Akar Kuning (Arcangelisia flava)

2019 ◽  
Vol 4 (2) ◽  
pp. 66-71
Author(s):  
Suratno Suratno ◽  
Muhammad Ikhwan Rizki ◽  
Mohammad Rizki Fadhil Pratama
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
Ic50 Values ◽  
Stem Extract ◽  
Ethanol Extracts

This study aims to determine the antioxidant activity of akar kuning (Arcangelisia flava) stem extract from Central Kalimantan using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. This research includes extraction of simplicia and test of antioxidant activity by DPPH method. Based on the analysis of antioxidant activity on ethanol extract from yellow root plant stem IC50 values of 136.81 ppm were obtained. This IC50 value indicates the antioxidant strength of the yellow root plant stems including in the moderate category. Further research on antioxidant activity in the fraction of akar kuning stems needs to be done to determine the composition of the antioxidant compounds in each solvent.

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