scholarly journals Isolasi dan Identifikasi Bakteri Resisten Arsen pada Sedimen di Pesisir Laut Buyat Tahun 2018

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Natalia M. Pantow ◽  
Billy J. Kepel ◽  
. Fatimawali

Abstract: Metals are very important and widely used in many aspects of human life, however, they can cause environmental pollution which is harmful to life. Arsenic is a very toxic metal that can cause kidney damage and is responsible to various chronic health problems, especially cancer. The coastal area of Buyat Sea in North Sulawesi Province has a high level of activity in mining industry. The mining waste results in environmental pollution due to arsenic release, thus triggers the occurence of arsenic-resistant bacteria. This study was aimed to determine whether there were arsenic-resistant bacteria in soil sediment originated from the coastal area of Buyat Sea and to identify the types of arsenic-resistant bacteria. This was a descriptive explorative study. Samples of soil sediment were obtained from one point on the coastal area of Buyat Sea. The results showed that there were 10 isolates of arsenic-resistant bacteria. There were five genera of bacteria that were resistant to arsenic, namely Escherichia, Klebsiella, Hafnia, Staphylococcus, and Bacillus. Conclusion: In the coastal area of Buyat Sea, five genera of arsenic-resistant bacteria were identified, as follows: Escherichia, Klebsiella, Hafnia, Staphylococcus, and Bacillus.Keywords: arsenic, sediment, arsenic-resistant bacteria Abstrak: Logam sangat penting dalam berbagai aspek kehidupan manusia walaupun dapat mencemari lingkungan dan berbahaya bagi kehidupan. Arsen merupakan logam berat yang sangat bersifat toksik dan dapat merusak ginjal serta bertanggung jawab terhadap berbagai gangguan kesehatan kronis, termasuk kanker. Daerah pesisir Buyat laut di Provinsi Sulawesi Utara memiliki tingkat aktivitas yang tinggi dalam bidang industri pertambangan. Limbah pertambangan mengakibatkan terjadinya pencemaran lingkungan oleh karena pelepasan arsen yang dapat memicu terjadinya bakteri resisten arsen. Penelitian ini bertujuan untuk mengetahui apakah terdapat bakteri resisten arsen pada sedimen yang berasal dari daerah pesisir laut Buyat dan untuk mengidentifikasi jenis bakteri resisten arsen yang ditemukan. Jenis penelitian ialah deskriptif eksploratif. Sampel penelitian ialah sedimen tanah di satu titik di pesisir laut Buyat. Hasil penelitian mendapatkan 9 isolat bakteri resisten arsen dan ditemukan lima genus bakteri yang resisten terhadap arsen yaitu Escherichia, Klebsiella, Hafnia, Staphylococcus, dan Bacillus. Simpulan: Di daerah pesisir laut Buyat ditemukan bakteri resisten arsen pada sedimen yaitu genus Escherichia, Klebsiella, Hafnia, Staphylococcus, dan Bacillus.Kata kunci: arsen, sedimen tanah, bakteri

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Tessalonicha J. Rompis ◽  
Widdhi Bodhi ◽  
Fona Budiarso

Abstract: Arsenic is still used in lots of human activities that results in environmental pollution. Arsenic is also found in nature in a small quantity. Gold mining industry is one of the human activities that uses arsenic. Mining waste that is not managed properly can inflict environmental pollution and harms the human health due to its toxic-substance content. This study was aimed to isolate and identify bacteria in the soil sediment obtained from the estuary area of Totok River, Southeast Minahasa and tested the bacteria's resistance levels to arsenic. Bacteria were isolated from sediment samples from the estuary area of Totok River, and the isolated bacteria were grown in agar media containing arsenic trioxide. Bacteria identification were perfomed by using morphological, physiological and biochemical tests. The results showed that bacteria grew successfully in 10 isolates; 2 isolates were Gram negative bacteria and 8 isolates were Gram positives bacteria. All isolates showed no motility. Identification of bacteria revealed four genera: Bacillus sp., Streptococcus sp., Acetobacter sp, and Escherichia sp that were resistant to arsenic 1000 ppm. Conclusion: There were 4 genera of arsenic-resistant bacteria identified in the sediment of the estuary area of Totok River, as follows: Bacillus sp., Streptococcus sp., Acetobacter sp, and Escherichia sp. All of them were resistant to arsenic 1000 ppm.Keywords: sediment, arsenic-resistant bacteria Abstrak: Arsen (As) masih banyak ditemukan dalam kegiatan manusia yang berakibat pencemaran terhadap lingkungan. Arsen juga dapat ditemukan di alam secara alami namun dalam jumlah yang sedikit. Industri pertambangan emas merupakan salah satu kegiatan manusia yang menggunakan arsen. Limbah pertambangan yang tidak dikelola dengan baik dapat mencemari lingkungan dan mengganggu kesehatan manusia karena berpotensi mengandung racun yang berbahaya. Penelitian ini bertujuan untuk mengidentifikasi bakteri resisten arsen serta tingkat resistensinya yang terdapat pada sedimen di muara Sungai Totok Kecamatan Ratatotok Kabupaten Minahasa Tenggara Sulawesi Utara. Bakteri diisolasi dari sedimen di muara sungai Totok dan ditumbuhkan pada media agar yang mengandung arsen trioksida. Identifikasi dari bakteri yang tumbuh menggunakan uji fisiologi, uji morfologi dan uji biokimia. Hasil penelitian mendapatkan 10 isolat dengan bakteri yang berhasil tumbuh, yaitu 2 isolat bakteri Gram negatif dan 8 isolat bakteri Gram positif. Semua isolat tidak menunjukkan adanya motilitas dan dapat dikategorikan ke dalam empat kelompok genus yaitu Bacillus sp., Streptococcus sp., Acetobacter sp, dan Escherichia sp. Keempat genus bakteri ini resisten terhadap arsen hingga konsentrasi 1000 ppm. Simpulan: Terdapat empat genus bakteri resisten arsen yang teridentifikasi dalam sedimen di muara Sungai Totok, yaitu Bacillus sp., Streptococcus sp., Acetobacter sp, dan Escherichia sp. Kesemuanya resisten terhadap arsen hingga konsentrasi 1000 ppm.Kata kunci: sedimen, bakteri resisten arsen


2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Muh. I. Hidayat ◽  
Aaltje Manampiring ◽  
Billy J. Kepel

Abstract: Arsenic is classified chemically as a metalloid which has both properties of metal and nonmetal. Production and application of arsenic in industries such as mining is a source of enviromental pollution. Therefore, agents for remediation process are needed. Arsenic resistant bacteria become a target of many studies related to its utilization as bioremediation agent. This study was aimed to obtain arsenic resistant bacteria identificated from soil sediment in the coastal area of Ratatotok Beach. This was an explorative descriptive study. Samples were colonies of arsenic resistant bacteria found in the soil sediment of the coastal area of Ratatotok Beach. The results of arsenic-resistant test showed that there were arsenic-resistant bacteria in every concentration. The morphological, physiological, and biochemical tests obtained four arsenic-resistant bacterial genus, namely Staphylococcus, Klebsiella, Hafnia, and Enterobacter. Conclusion: Four genera of arsenic-resistant bacteria identified in the sediment of the coastal area of Ratatotok Beach, as follows: Staphylococcus, Klebsiella, Hafnia, and Enterobacter.Keywords: arsenic, sediment, arsenic resistant bacteria Abstrak: Arsenik diklasifikasikan secara kimia sebagai metaloid yaitu memiliki kedua sifat logam dan bukan logam. Produksi dan penggunaan arsen di dalam kegiatan industri seperti industri pertambangan, merupakan salah satu sumber pencemarannya di lingkungan. Arsen merupakan polutan bagi lingkungan sehingga diperlukan suatu agen untuk proses remediasi. Bakteri resisten arsen menjadi target dari banyak penelitian dalam rangka pemanfaatannya sebagai agen bioremediasi. Penelitian ini bertujuan untuk mengetahui jenis bakteri resisten arsen yang teridentifikasi pada sedimen tanah di pesisir pantai Ratatotok. Jenis penelitian yaitu dekriptif eksploratif. Sampel yang digunakan yaitu koloni bakteri resisten arsen yang terdapat dalam sedimen tanah pesisir pantai Ratatotok. Hasil uji resistensi arsen mendapatkan adanta bakteri resisten arsen pada setiap konsentrasi uji. Setelah dilakukan uji morfologi, fisiologi, dan biokomia didapatkan empat genus bakteri yang resisten terhadap arsen: Staphylococcus, Klebsiella, Hafnia, dan Enterobacter. Simpulan: Terdapat empat genus bakteri resisten arsen yang teridentifikasi dalam sedimen tanah pesisir pantai Ratatotok, yaitu Staphylococcus, Klebsiella, Hafnia, dan Enterobacter.Keyword: arsen, sedimen tanah pesisir, bakteri resisten arsen


Author(s):  
В.Б. Заалишвили ◽  
О.Г. Бурдзиева ◽  
А.С. Кануков ◽  
Л.В. Дзобелова

В последние годы наблюдается значительный рост численности населения, которому соответствуют ускоренные темпы индустриализации. Это сопровождается всё более значительным загрязнением окружающей среды тяжелыми металлами. Наибольший вклад в загрязнение окружающей среды Республики Северная Осетия – Алания долгие годы вносила горнодобывающая промышленность, в последнее же время к ней, в качестве не менее весомого источника загрязнения присоединились нестационарные источники. Загрязнение тяжелыми металлами создает серьезную опасность для здоровья населения и окружающей среды из-за их токсичной природы. Цель исследования. Разработка карт пространственного распределения загрязняющих веществ является основой в оценке уровня загрязнения окружающей среды. Цель настоящего исследования – разработка карт индекса канцерогенного риска и установление его взаимосвязи с онкозаболеваемостью населения. Методы исследования. В процессах создания карт используются методы интерполяции, так как количество проб и измерений содержания вредных веществ в той или иной среде, как правило, весьма ограничено. Согласно зарубежным исследованиям, для большинства наиболее часто используемых методов интерполяции обеспечивается одинаково высокая точность. В настоящей работе при расчёте индексов канцерогенного риска использовался метод обратного взвешивания расстояний. Результаты работы Отобран материал по заболеваемости населения злокачественными образованиями по различным районам г. Владикавказа. Основной удельный вес вновь зарегистрированных злокачественных новообразований приходится на возрастные группы свыше 60 лет. Анализ онкозаболеваемости показал, что в тех районах г. Владикавказа, где уровень загрязнения окружающей среды выше, выше и показатели онкозаболеваемости. Проведен расчёт индекса риска для здоровья населения при воздействии химических веществ загрязняющих окружающую среду. По всем исследуемым показателям наблюдается значительное превышение предельно допустимых значений, за исключением ртути. Полученные данные о высоком уровне канцерогенного риска согласуются с высоким уровнем онкологической заболеваемости на территории города, что говорит о тесной взаимосвязи заболеваемости и индекса канцерогенного риска. In recent years, there has been a significant increase in the population, which is matched by the accelerated rate of industrialization. This is accompanied by increasingly significant pollution of the environment with heavy metals. For many years, the mining industry has made the greatest contribution to the environmental pollution of the Republic of North Ossetia – Alania, non-stationary sources have recently joined as an equally significant source of pollution. Heavy metal pollution poses a serious threat to public health and the environment due to its toxic nature. Aim. The development of maps of the spatial distribution of pollutants is the basis for assessing the level of environmental pollution. The aim of this study is to develop maps of the carcinogenic risk index and to establish its relationship with the incidence of cancer in the population. Methods. In the process of creating maps, interpolation methods are used, since the number of samples and measurements of the content of harmful substances in a particular environment is usually very limited. According to foreign studies, for most of the commonly used interpolation methods, equally high accuracy is provided. In the present work, we used the method of the inverse weighting of distances for calculating the indices of carcinogenic risk. Results. The material on the incidence of malignant neoplasms in the population in various districts of Vladikavkaz was selected. The main share of newly registered malignant neoplasms falls on the age groups over 60 years old. The analysis of cancer morbidity showed that in those areas of Vladikavkaz where the level of environmental pollution is higher, the indicators of cancer morbidity are also higher. The calculation of the risk index for public health under the influence of chemical substances polluting the environment has been carried out. For all the studied indicators, there is a significant excess of the maximum permissible values, with the exception of mercury. The obtained data on the high level of carcinogenic risk is consistent with the high level of cancer incidence in the city; it indicates a close relationship between the incidence and the carcinogenic risk index.


1996 ◽  
Vol 451 ◽  
Author(s):  
Guen Nakayama ◽  
Yuichi Fukaya ◽  
Masatsune Akashi

ABSTRACTIn the scheme for geological disposal of high level radioactive nuclear wastes, the burial pit is to be isolated from the sphere of human life by a multiple-barrier system, which consists of an artificial barrier, composed of a canister, an overpack and a bentonite cushioning layer, and a natural barrier, which is essentially the bedrock. As the greatest as well as essentially the sole detriment to its integrity would be corrosion by groundwater. The groundwater comes to it seeping through the bentonite zone, thereby attaining conceivably the pH of transition from general corrosion to passivity, pHd, the behaviors of mild steel in such a groundwater environment have been examined. It has been shown that the pHd is lowered (enlargement of the passivity domain) with rising temperature and carbonate-bicarbonate concentration, while it is raised (enlargement of the general corrosion region) with increasing concentrations of chloride and sulfate ions.


2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Irma Maria Lontoh ◽  
Jenny Hilda Pakasi ◽  
Martha Salea-Warouw

The fishermen community is a community found in North Sulawesi, especially in the coastal area of Sario-Malalayang, Manado. With different cultural backgrounds, ethnicities, this group has its own uniqueness, especially in language. This research succeeded is finding expressions in the fishermen community in the coastal area of Sario-Malalayang. These expressions consist of forms of words, phrases, and clauses in accordance with the lingual forms of theory from Widdowson (1997: 3). The results of the study found a number of lingual forms in affixed words, which had a similar prefix as in the words ba-daseng, ba-kintu, ba-tono, ba-saoh, ba-pake. In addition, the majority of phrases contained in the results of the study are predominantly dominated by noun phrases, such as in the lips phrases of napo, puru loe, coolies, ikang itching, mulu sosoroka, gargantang tubir.This research also succeeded in exploring and discovering cultural meanings. The meanings in the form of words, such as ‘badaseng’, ‘sunga’, then the form of a phrase, like ‘puru loe’, ‘mulu sosoroka’, then sentence expressions in the form of expressions like ‘udang deng katang so kurang sama’, ‘kase wora mar jang talapas’, ‘karja cuma sampe di kuli aer’, etc. The expressions encountered generally contain various cultural meanings, namely advice, satire, ridicule, seduction, warning, insults, and despair. Based on the conclusion, the researcher suggests to the next researcher, to be more comprehensive related to the use of Manado Malay language expression in different background and contexts in order to get a whole description about expressions system of Manado Malay language, especially in the fishermen community in North Sulawesi.Keywords: fishermen community, language and culture


Author(s):  
Zhengjie Gao ◽  
Dayi He ◽  
Shuaifang Niu

Enterprise environmental performance has causal complexity. The purpose of this paper is to discover the possible combination of conditions for enterprises to achieve high environmental performance. Based on the resource dependence theory, stakeholder theory, and externality theory, this paper constructs the theoretical framework of enterprise environmental performance evaluation and applies the fsQCA method to study the major influencing factors and mechanism of the environmental performance of listed enterprises in the Chinese mining industry. Based on the data from 2016 to 2019, the results show that there are four configurations of multiple factors leading to high environmental performance. Based on these configurations, three possible paths, internally driven, internally–externally driven, and externally driven, are established to improve environmental performance. Further, we also find that, between profitability and government regulation and between enterprise size and board independence are interchangeable condition variables; public attention outweighs other factors for Chinese mining enterprises. Countermeasures and suggestions from perspectives of government supervision, public concern, and enterprise internal governance are proposed at the end the study.


1992 ◽  
Vol 6 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Shigeru Maeda ◽  
Akira Ohki ◽  
Kuniaki Miyahara ◽  
Kensuke Naka ◽  
Shiro Higashi

Author(s):  
G.S. Agzamova ◽  
◽  
N.U. Ibragimova ◽  
Yu.A. Abdieva ◽  

Abstract: Protecting and promoting the health of workers in the mining industry is one of the most important problems of occupational pathology and health care. The structure and levels of prevention of occupational diseases are directly dependent on harmful and adverse factors of the production environment and labor process, adequately reflecting the state of production. Purpose: to study the issues of prevention of occupational and production-related diseases of mining and metallurgical plant workers. Research materials and methods: a dynamic observation of the health status of workers in the main industries of the mining and metallurgical plant (800 workers) was carried out. 92 patients with silicosis were examined. Results: Up to 92.8% of first-time occupational diseases are detected during periodic medical examinations. The prevailing sociomatic pathology is cardiovascular pathology, namely, arterial hypertension and diseases of the musculoskeletal system, mainly osteochondrosis of the spine. Prevalence of silicosis was observed in individuals with little professional experience (from 5 years old), young age and primary detection of patients in stage II silicosis, which was accompanied by respiratory failure. Conclusions: The prevention programme developed will ensure a high level of health care in terms of early diagnosis, rehabilitation and secondary prevention of both occupational and occupational diseases.


Author(s):  
Marta Makara-Studzińska ◽  
Kornelia Zaręba ◽  
Natalia Kawa ◽  
Dorota Matuszyk

SARS-CoV-2 coronavirus emerged in the world at the end of 2019. The introduction of a number of restrictions had a significant effect on numerous aspects of human life with particular influence being exerted on pregnant women and their sense of security. The study aimed to assess the level of anxiety and its main determinants in women in the third trimester of pregnancy during the coronavirus pandemic. The study technique included the present purposely designed questionnaire, Labor Anxiety Questionnaire (KLPII), and the State-Trait Anxiety Inventory (STAI). The study was conducted in a group of 315 women in the third trimester of pregnancy. A total of 258 women (81.9%) completed the questionnaire in May 2020, and 57 of them (18.1%) completed it in October 2020. The overall analysis of the Labor Anxiety Questionnaire and the STAI inventory revealed a high level of anxiety, particularly situational anxiety, in pregnant women during the SARS-CoV-2 pandemic. The age and financial status of the women were the factors which contributed to the intensification of tokophobia. Women interviewed in October 2020 were characterized by higher tokophobia levels compared to the respondents included in May 2020. It seems justified to in-crease the vigilance in the diagnostics of possible mental disorders in the perinatal period during pandemic.


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