scholarly journals Pengaruh cabe rawit terhadap gambaran histopatologik lambung tikus Wistar yang diinduksi aspirin

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abel T. Bawulele ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Control Group ◽  
Histopathological Changes ◽  
Acute Gastritis ◽  
Group B ◽  
Group D ◽  
Mucosa Epithelium

Abstract: Cayenne pepper (Capsicum frustescens L ) is commonly in daily food. This study aimed to determine the effect of cayenne pepper to histopathological changes in gaster of Wistar rats induced by aspirin. This was an experimental study. Subjects were Wistar rats consisted of 5 rats as the negative control and 20 rats as the treatment group. Group A, the negative control group, was untreated; group B was treated with aspirin 21mg/day for 10 days; group C was treated with aspirin 21mg/day for 10 days together with cayenne pepper 84mg once daily; group D was treated with aspirin 21mg/day for 10 days followed by cayenne 84mg twice daily; and group E was treated with aspirin 21mg/day for 10 days and then was untreated for 3 days. The histopathological changes in group C showed a lot of inflammatory cells and erosion of gastric mucosa epithelium compared to group B, D, and E. Group D also showed inflammatory cells but no erosion as in group C. Group E had less inflammatory cells than group B, C, and D. Conclusion: Administration of aspirin 21mg/kg BW for 10 days led to acute gastritis in Wistar rat. Administration of aspirin together with cayenne 84mg/day led to acute gastritis and erosion of gastric mucosa epithelium. However, administration of cayenne pepper 168mg/day after aspirin led to acute gastritis without erosionKeywords: pepper, gaster, aspirinAbstrak : Cabe Rawit (Capsicum frustescens L.) banyak dijumpai dalam makanan sehari-hari. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian cabe rawit terhadap gambaran histopatologik lambung tikus wistar yang diinduksi aspirin. Jenis penelitian ialah eksprimental. Subjek penelitian ialah tikus wistar, yang dibagi atas: 5 ekor untuk kontrol negatif dan 20 ekor untuk perlakuan. Kelompok A (kontrol negatif) tidak diberi perlakuan; kelompok B diberikan aspirin 21mg/hari selama 10 hari; kelompok C diberikan aspirin 21mg/hari selama 10 hari bersama cabe rawit 84mg sekali sehari; kelompok D diberikan aspirin 21mg/hari selama 10 hari kemudian dilanjutkan pemberian cabe rawit 84mg selama 3 hari 2 kali sehari; dan kelompok E diberikan aspirin 21mg/hari selama 10 hari kemudian tidak diberi perlakuan selama 3 hari. Hasil penelitian menunjukkan bahwa pada kelompok C terlihat banyak sel-sel radang disertai erosi epitel mukosa lambung dibanding dengan kelompok B, D, dan E. Pada kelompok D pemberian aspirin dilanjutkan dengan cabe rawit dosis 2 kali sehari masih terdapat sel-sel radang tetapi tidak terjadi erosi seperti pada kelompok C. Kelompok E memiliki sel-sel radang yang lebih sedikit dibandingkan kelompok B, C, dan D. Simpulan: Pemberian aspirin 21mg/hari selama 10 hari menyebabkan gastritis akut pada tikus wistar. Pemberian aspirin bersama cabe rawit 84mg/hari menyebabkan gastritis akut erosi sedangkan pemberian cabe rawit 168mg/hari setelah diberi aspirin menyebabkan gastritis akut tanpa erosi.Kata kunci: cabe rawit, lambung, aspirin

scholarly journals EFEK PEMBERIAN EKSTRAK KAYU MANIS (CINNAMOMUM BURMANNII) TERHADAP GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR YANG DIBERI ASPIRIN

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Janet Walangitan ◽  
Lily Loho ◽  
Meilany Durry
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Control Group ◽  
Therapeutic Effects ◽  
Cinnamon Extract ◽  
Group A ◽  
Cinnamomum Burmannii ◽  
Group B ◽  
Group D

Abstract: Cinnamon (Cinnamomum burmannii) is a traditional herbal plants which are often found in our daily life and has many benefits especially in health. This study were designed to know the effect of cinnamon on gastric mucosa given aspirin. This was an experimental research and used Wistar rats as the subject research.  The Wistar rats were randomly divided into 6 mice of control group and 9 mice of treatment group. Group A (K-) given pellets, group B (K+) given pellets and aspirin 150 mg/kgBB for 7 days, group C given pellets, aspirin 150 mg/kgBB and cinnamon extract 3 mg for 3 days, group D given pellets, aspirin 150 mg/kgBB for 7 days and given cinnamon extract 3 mg for 3 days, group E given pellets, aspirin 150 mg/kgBB for 7 days and given pellets only (without treatment) for 3 days. The results showed that aspirin cause gastric mucosa damage in group B (K+) compared with kelompok A (K-). Group C and D showed less inflammatory cells compared with group B (K+). Group E showed more inflammatory cells compared with group D. The study suggested that cinnamon extract has protective and therapeutic effects on gastric mucosa of Wistar rats. Keywords: cinnamon, gaster, aspirin.   Abstrak: Kayu manis (Cinnamomum burmannii) merupakan tanaman herbal tradisional yang banyak dijumpai dalam kehidupan sehari- hari dan memiliki banyak manfaat termasuk dalam bidang kesehatan. Penelitian ini bertujuan untuk mengetahui apakah ada efek pemberian kayu manis terhadap mukosa lambung yang diberi aspirin. Desain Penelitian yang digunakan adalah metode eksperimental dan subjek penelitian menggunakan tikus Wistar, yang terbagi atas 6 ekor kontrol dan 9 ekor perlakuan. Kelompok A (K-) diberikan pelet selama 7 hari, kelompok B (K+) diberikan pelet dan aspirin 150mg/kgBB selama 7 hari, kelompok C diberikan pelet, aspirin 150 mg/kgBB, ekstrak kayu manis 3 mg secara bersama- sama selama 7 hari, kelompok D diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian ekstrak kayu manis 3 mg selama 3 hari, kelompok E diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian pelet saja (tanpa perlakuan) selama 3 hari. Hasilnya menunjukkan aspirin menimbulkan kerusakan mukosa lambung pada kelompok B (K+) dibandingkan dengan kelompok A (K-). Kelompok C dan D menunjukkan sel-sel radang yang lebih sedikit dari kelompok B(kontrol +). Kelompok E menunjukkan sel- sel radang yang lebih banyak dibandingkan dengan kelompok D. Kesimpulan dari penelitian ini adalah kayu manis mempunyai efek protektif dan terapeutik terhadap mukosa lambung tikus Wistar. Kata kunci: kayu manis, lambung, aspirin.

scholarly journals GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR (Rattus norvegicus) YANG DIBERIKAN LENGKUAS (Alpinia galanga Willd) SETELAH DIINDUKSI OLEH ASAM MEFENAMAT

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Juita Pasaribu ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Wistar Rats ◽  
Acute Inflammation ◽  
Inflammatory Cells ◽  
Control Group ◽  
Treatment Groups ◽  
Acute Gastritis ◽  
Group A ◽  
Mucosal Lining ◽  
Group B ◽  
Group D

Abstrak: Gastritis akut merupakan proses inflamasi yang bersifat akut dan biasanya terjadi pada bagian permukaan mukosa lambung. Penyakit ini biasanya disebabkan karena banyak faktor salah satunya yaitu penggunaan asam mefenamat dalam dosis yang berlebihan. Pada penelitian yang telah dilakukan sebelumnya didapatkan bahwa lengkuas dapat mengurangi terjadinya radang akut. Tujuan penelitian ini adalah untuk mengetahui gambaran histopatologi lambung tikus wistar yang diberikan lengkuas setelah diinduksi dengan asam mefenamat. Penelitian ini menggunakan metode eksperimental dengan sampel 11 ekor tikus wistar yang dibagi dalam 4 kelompok perlakuan. Kelompok A tidak diberikan perlakuan. Kelompok B diberikan asam mefenamat 10 mg selama 7 hari. Kelompok C diberikan asam mefenamat 10 mg selama 7 hari kemudian diberikan perasan lengkuas selama 7 hari. Kelompok D diberikan asam mefenamat 10 mg kemudian tidak diberikan perlakuan selama 7 hari. Hasil penelitian ini menunjukkan bahwa gambaran mikroskopik lambung tikus wistar pada kelompok C terdapat sel-sel radang PMN yang lebih sedikit dibandingkan dengan kelompok B dan D. Simpulan: lengkuas dapat mengurangi sel-sel radang PMN pada mukosa lambung yang diberikan dengan asam mefenamat. Kata kunci: Lengkuas, Asam Mefenamat, Gastritis akut.   Abstract : acute gastritis is an acute inflammatory process normally occurring in the mucosal lining of the stomach. This disease is caused by a variety of factors, one of which is the use of mefenamat acid in large doses. Previous research has revealed that galangal can be used to reduce acute inflammation. The objective of this research was to reveal the histopathological pictures of stomach of wistar rats that treatment with galangal after induce by mefenamat acid. This study is an experimental, employing 11 wistar rats which were assigned for four treatment groups. Group A was the control group in which the rats received no treatment. Rats in group B were administered mefenamat acid 10 mg for 7 days. In group C, the rats were also administered mefenamat acid 10 mg for 7 days and then received galangal distillation for 7 days. In group D, rats were administered mefenamat acid 10 mg for 7 days and then for 7 days without receiving anymore mefenamat acid or galangal distillation. Results showed that microscopic pictures of stomach of wistar rats in group C were presented with PMN inflammatory cells fewer than rats in group B and D. Conclusion: galangal can reduce PMN inflammatory cells in mucosal lining of the stomach exposed to mefenamat acid. Key words: galangal, mefenamat acid,  acute gastritis.

scholarly journals GAMBARAN HISTOPATOLOGIK LAMBUNG TIKUS WISTAR YANG DIBERIKAN BUAH PEPAYA SEBELUM INDUKSI ASPIRIN

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Vallery Jerry Nanlohy
Keyword(s):  
Wistar Rats ◽  
Qualitative Change ◽  
Negative Control Group ◽  
Negative Control ◽  
Lower Degree ◽  
Control Group ◽  
Treatment Groups ◽  
Acute Gastritis ◽  
Group B ◽  
Group D

Abstract: Aspirin contains acetyl salicylic acid which can cause qualitative change on the gastric mucosa. The active ingredients of papaya can increase immunity and protect the stomach from the use of aspirin and steroid. Objective: To reveal the histophatological features of the stomach of Wistar rats administered with papaya juice before aspirin induction. Method: This experimental research employed 20 wistar rats which were divided into the negative control group (A) and treatment groups. All rats were fed with regular or standard pellets for 10 days. Treatment groups were classified into four groups; rats were given papaya juice of 0.8 cc/day (group B), rats were administered aspirin of 30 mg/day (group C), rats were given papaya juice 0.8 cc/day before induced with aspirin (group D), and rats were given papaya juice 2.4 cc/day before induced with aspirin of 30 mg/day (group E). On the eleventh day, the rats were terminated to isolate the stomach followed by macroscopic and microscopic examinations. Results: It was found that the microscopic features were normal in negative control group and group B. In group C, histological examination showed acute gastritis, i.e.,inflammatory cells, edema mucosa and capillary dilatation. Rats in group D and E showed lower degree of acute gastritis compared with group C. Conclusions: Administration of papaya juice for 10 days before aspirin  is given may reduce the signs of acute gastritis as demonstrated by histopathological features of the stomach of wistar rats. Administration of papaya juice of 2.4 cc/day result in lower degree of gastritis compared with recommended dose of papaya juice (0.8 cc/day). Keywords: Aspirin, papaya, stomach.   Abstrak: Aspirin mengandung asam asetil salisilat  yang dapat menimbulkan perubahan kualitatif mukus lambung. Bahan aktif buah pepaya dapat meningkatkan kekebalan dan melindungi lambung dari penggunaan obat aspirin dan steroid. Tujuan: Untuk mengetahui gambaran histopatologik lambung tikus wistar yang diberikan jus buah pepaya sebelum induksi aspirin. Metode: Penelitian eksperimental ini menggunakan 20 ekor tikus wistar yang dibagi dalam kelompok kontrol negatif (A) dan kelompok perlakuan yang diberikan diet pellet standar selama 10 hari. Kelompok perlakuan dibagi dalam empat kelompok, yaitu  kelompok  yang diberikan jus pepaya 0,8 cc/hari (B), kelompok  yang diberikan aspirin 30 mg/hari (C),  kelompok  yang diberikan jus pepaya 0,8 cc/hari sebelum diinduksi aspirin (D), dan kelompok  yang diberikan jus pepaya 2,4 cc/ hari sebelum diinduksi aspirin 30 mg/hari (E). Pada hari ke-11 Tikus wistar diterminasi  untuk diambil lambungnya yang kemudian diamati secara makroskopik dan mikroskopik. Hasil: Penelitian ini menunjukkan gambaran mikroskopik yang normal pada kelompok kontrol negatif dan perlakuan B. Pada kelompok perlakuan C menunjukkan gambaran histologik gastritis akut, yaitu sel-sel radang, edema mukosa, dan pelebaran kapiler. Pada kelompok perlakuan D dan E menunjukkan gambaran histologi gastritis akut yang lebih ringan dibandingkan dengan kelompok perlakuan C. Simpulan: Pemberian jus buah pepaya sebelum pemberian aspirin selama 10 hari dapat mengurangi tanda-tanda gastritis akut yang terlihat pada gambaran histopatologik lambung tikus wistar. Pemberian jus buah pepaya sebanyak 2,4 cc/hari menghasilkan gambaran gastritis  yang lebih ringan dibandingkan dengan pemberian dosis anjuran (0,8 cc/hari) jus buah pepaya. Kata Kunci: Aspirin, pepaya, lambung.

scholarly journals Gambaran histopatologik lambung tikus wistar (Rattus norvegicus) yang diberi perasan umbi bengkuang (Pachyrhizus erosus (L) Urban) setelah induksi aspirin

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Debora R. Mentang ◽  
Lily L. Loho ◽  
Poppy M. Lintong
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Acute Inflammation ◽  
Inflammatory Cells ◽  
Pachyrhizus Erosus ◽  
Acute Gastritis ◽  
Experimental Laboratory ◽  
Inflammatory Drugs ◽  
Group B ◽  
Group D

Abstract: Acute gastritis is an acute inflammation of the gastric mucosal and submucosal. This disease is caused by a variety of factors, one of which is the use of non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin. Aspirin is one of the salicylate group that is irritating to the stomach. Yam stimulate the growth of epithelial cells also repair the surface of cells in the gastric mucosal. This research aims to describe histopathologic Wistar rats were given gastric juice yam tubers after induction aspirin. This is an experimental laboratory study conducted in September 2014 until January 2015 with a sample of 20 Wistar rats were divided into 4 groups. A group given no treatment. Group B was given aspirin 150 mg for 10 days. Group C was given aspirin 150 mg for 10 then given yam tuber juice for 3 days. Group D was given aspirin 150 mg for 10 days and then given pellets for 3 days. Results showed that microscopic pictures of stomach of wistar rats in group C were presented with PMN inflammatory cells fewer than rats in group B and D. Conclusion: Aspirin showed signs of acute gastritis and giving yam tuber juice of showed inflammation-inflammatory less than without giving juice of yam tubers.Keywords: bengkoang, aspirin, acute gastritisAbstrak : Gastritis akut adalah inflamasi akut pada mukosa dan submukosa lambung. Penyakit ini biasanya disebabkan karena banyak faktor salah satunya yaitu penggunaan obat non steroid anti inflamasi (NSAID) seperti aspirin. Aspirin merupakan salah satu dari golongan salisilat yang bersifat iritatif terhadap lambung. Bengkuang merangsang pertumbuhan sel-sel epitel juga perbaikan permukaan sel pada mukosa lambung. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik lambung tikus wistar yang diberi perasan umbi bengkuang setelah induksi aspirin. Penelitian ini bersifat eksperimental laboratorik yang dilakukan pada bulan September 2014 sampai Januari 2015 dengan sampel 20 ekor tikus wistar yang dibagi dalam 4 kelompok perlakuan. Kelompok A tidak diberikan perlakuan. Kelompok B diberikan aspirin 150 mg selama 10 hari. Kelompok C diberikan aspirin 150 mg selama 10 kemudian diberikan perasan umbi bengkuang selama 3 hari. Kelompok D diberikan aspirin 150 mg selama 10 hari kemudian diberikan pelet selama 3 hari. Hasil penelitian ini menunjukkan bahwa gambaran mikroskopik lambung tikus wistar pada kelompok C terdapat sel-sel radang PMN yang lebih sedikit dibandingkan dengan kelompok B dan D. Simpulan: Pemberian aspirin menunjukkan tanda-tanda gastritis akut dan pemberian perasan umbi bengkuang menunjukkan radang-radang lebih sedikit dibandingkan tanpa pemberian perasan umbi bengkuang.Kata kunci: bengkuang, aspirin , gastritis akut

scholarly journals Gambaran histopatologik hati tikus Wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi karbon tetraklorida (CCl4)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Satriani Syahrin ◽  
Carla Kairupan ◽  
Lily Loho
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Moringa Leaf Extract

Abstract: Moringa (Moringa oleifera) is a traditional plant, which is known to treat liver disease. The effect of moringa is related to the antioxidant activity of its compounds such as quercetin and flavonoid silymarin which are useful as liver protector. Carbon tetraclorida is a hepatotoxic substance that is usually used as an inducer of liver damage in studies related to hepatoprotector activity of a substance. This study aimed to describe liver histopathological features of Wistar rats fed with moringa leaf extract after CCl4 induction. This was an experimental study using 24 Wistar rats (Rattus norvegicus) which were divided into 4 groups. Group A, the negative control, was terminated at day 6th, and the other groups (B, C, and D) were induced with CCl4 0.05 cc/day for 5 days. After CCl4 induction, group B were terminated at day 6th; group C was treated with moringa leaf extract 100 mg/day for 5 days and was terminated at day 11th; group D received no treatment for 5 days and was terminated at day 11th. The results showed that groups induced with CCl4 for 5 days showed the presence of inflammatory cells and fatty cells. The groups treated with moringa leaf extract 100mg/day for 5 days after CCl4 induction 0.05 cc/day exhibited regeneration of liver cells in nearly all lobules. Conclusion: Administration of moringa leaf extract of 100mg/day could accelerate liver cell regeneration of Wistar rats after induction of CCl4 0.05cc/day.Keywords: moringa leaf extract, carbon tetrachloride, histopathologogical image of liverAbstrak: Kelor merupakan tanaman tradisional yang diketahui dapat mengobati penyakit hati. Khasiat obat tanaman kelor dihubungkan dengan kandungan senyawa kimia quercetin dan silymarin golongan flavonoid dengan aktivitas antioksidan yang dapat melindungi dan mengobati kerusakan hati. Karbon tetraklorida merupakan zat hepatotoksik yang lazim dipakai sebagai penginduksi kerusakan hati dalam pengujian aktivitas hepatoprotektor suatu zat. Penelitian ini bertujuan untuk mengetahui gambaran histopatologik hati tikus wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi CCl4. Jenis penelitian ini ialah eksperimental menggunakan 24 ekor tikus Wistar spesies Rattus norvegicus yang dibagi dalam 4 kelompok. Kelompok A merupakan kelompok kontrol negatif, diterminasi pada hari ke-6; kelompok B,C, dan D (kelompok perlakuan) diberi CCl4 dengan dosis 0,05 cc/hari selama 5 hari. Setelah pemberian CCl4, kelompok B langsung diterminasi pada hari ke-6; kelompok C diberi ekstrak daun kelor 100 mg/hari selama 5 hari, diterminasi pada hari ke-11; kelompok D tidak diberi perlakuan selama 5 hari, diterminasi pada hari ke-11. Hasil penelitian menunjukkan bahwa kelompok tikus yang diinduksi CCl4 selama 5 hari secara histopatologik memperlihatkan sel-sel radang dan perlemakan sel. Kelompok tikus yang diberi ekstrak daun kelor 100 mg/hari selama 5 hari setelah diinduksi CCl4 0,05 cc/hari menunjukkan regenerasi sel hati, hampir di seluruh lobuli hati. Simpulan: Pemberian ekstrak daun kelor 100 mg/hari setelah induksi CCl4 0,05 cc/hari dapat mempercepat regenerasi sel hati tikus Wistar yang mengalami cedera akibat CCl4.Kata kunci: ekstrak daun kelor, karbon tetraklorida, gambaran histopatologik hati

scholarly journals Gambaran histopatologik lambung tikus Wistar (Rattus norvegicus) yang diinduksi asam mefenamat dan diberi susu kental manis

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Aisyah N. Shafira ◽  
Carla F. Kairupan ◽  
Meilany F. Durry
Keyword(s):  
Mefenamic Acid ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Epigastric Pain ◽  
Negative Control ◽  
Control Group ◽  
Histopathological Features ◽  
Condensed Milk ◽  
Acute Gastritis ◽  
Group B

Abstract: Empirically, sweet condensed milk is often consumed by gastritis patients with acute gastritis to relieve the symptoms of epigastric pain. Its amphoteric acidity (pH 6,5 - 6,7), sweet taste, nutritional and oligosaccharides contents are some factors that are predicted to influence the relieve of epigastric pain. Acute gastritis can be induced by NSAIDs, such as mefenamic acid. This study aimed to reveal the histopathological features of the gaster of Wistar rats (Rattus norvegicus) induced with mefenamic acid and fed with sweet condensed milk. This was laboratory experimental study using 21 Wistar rats. Rats were divided into three groups (seven rats in each group). Group A was the negative control, group B was induced with mefenamic acid 23,25 mg/day for seven days, and group C were induced with mefenamic acid 23,25 mg/day and administered with sweet condensed milk 0,8 ml/day simultaneously for seven days. All rats were terminated on day 8. The results showed that histopathological features of the gaster of Wistar rats had less inflammatory cells and more regenerated cells than that of rats in group B. Conclusion: Histopathological features of gaster of Wistar rats induced with mefenamic acid and treated with sweet condensed milk showed milder signs of acute gastritis and better cell regeneration than that of Wistar rat not treated with sweet condensed milk.Keywords: Sweet Condensed Milk, Acute Gastritis, Histopathological Abstrak: Berdasarkan pengalaman empiris, susu kental manis sering dikonsumsi oleh penderita gastritis akut untuk meredakan keluhan nyeri epigastrium pasien. Sifat amfoter (pH 6,5 – 6,7), rasa manis, kandungan nutrisi, dan oligosakarida susu merupakan faktor-faktor yang diduga berpengaruh terhadap perbaikan keluhan tersebut. Penyakit gastritis akut dapat diinduksi dengan obat AINS, salah satunya asam mefenamat. Tujuan penelitian ini ialah untuk mengetahui gambaran histopatologik lambung tikus wistar (Rattus norvegicus) yang diinduksi asam mefenamat dan diberi susu kental manis. Penelitian eksperimental ini menggunakan subyek 21 ekor tikus wistar yang dibagi dalam tiga kelompok (tujuh ekor tikus setiap kelompok). Kelompok A tidak diberi perlakuan, kelompok B diberi asam mefenamat 23,25 mg/tikus/hari selama tujuh hari, dan kelompok C diberi asam mefenamat 23,25 mg/tikus/hari dan susu kental manis 0,8 ml/tikus/hari secara bersamaan selama tujuh hari. Semua tikus diterminasi pada hari ke-8. Hasil penelitian ini menunjukkan gambaran histopatologik lambung tikus wistar berupa infiltrat sel-sel radang yang lebih sedikit serta sel-sel regenerasi yang lebih aktif dan banyak pada kelompok C jika dibandingkan dengan yang terlihat pada kelompok B. Simpulan: Gambaran histopatologik lambung tikus wistar yang diinduksi asam mefenamat dan diberi susu kental manis menunjukkan tanda-tanda gastritis akut yang lebih ringan dan regenerasi sel yang lebih baik dibandingkan dengan yang terlihat pada lambung tikus wistar yang diinduksi asam mefenamat tetapi tidak diberi susu kental manis. Kata kunci: Susu Kental Manis, Gastritis Akut, Histopatologik

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

scholarly journals Anti-inflammatory activity of acacia catechu-bark aqueous solution in aspirin induced gastric ulcer in rodents

2021 ◽  
Vol 8 (12) ◽  
pp. 5649
Author(s):  
Uzma Waseem ◽  
Syeda Rizwana Jafri ◽  
Sarah Khalid ◽  
Fauzia Qureshi ◽  
Nadia Majeed ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Inflammatory Cells ◽  
Protective Role ◽  
Gastric Injury ◽  
Medical Institute ◽  
Control Group ◽  
Albino Rats ◽  
Anti Inflammatory ◽  
Group B ◽  
Acacia Catechu

Background: Aspirin is amongst the most widely used drugs and has many adverse effects on gastric mucosa. Anti-inflammatory properties of Acacia catechu have been established already. Objective was to evaluate the histopathological changes induced by aspirin in the stomach of albino rats and to assess the protective effect of different doses of Acacia catechu.Methods: Experimental study Postgraduate Medical Institute, Lahore for 21 days. Forty-eight adult albino rats, both males and female, were divided into four groups A, B, C and D randomly; each comprising of 12 rats. Group A was control, group B was given aspirin 100 mg/kg and group C and D were given aspirin 100 mg/kg along with Acacia catechu 250 mg/kg and 500 mg/kg respectively by oral route. The rats from individual group were sacrificed on 3rd day, 7th day and 14th day and stomachs were examined under light microscope to observe the inflammatory cells infiltration.Results: Gross and microscopic findings on days 3, 7 and 14 were similar. Control groups A1, A2 and A3 showed normal healthy gastric mucosa and the least number of inflammatory cells. In group B, aspirin produced ulcerations and linear breaks; with highest inflammatory infiltrates. On microscopic examination, numerous inflammatory cells were noted. Group C and D rats had minimum ulcer index and fewer inflammatory cells.Conclusions: Acacia catechu has protective role against gastric injury by inhibiting inflammation. 

The Effects of Moxibustion on Serum Interleukins and T Cell Subset in H.polyri Infected Rats

2015 ◽  
Vol 3 (1) ◽  
pp. 38-45
Author(s):  
Jing Shen ◽  
Yan Peng ◽  
Dong-Mei Shi ◽  
Yin-Shuai Feng ◽  
Yan-Ling Hou ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Control Group ◽  
Intragastric Administration ◽  
Therapeutic Effects ◽  
Model Group ◽  
Tnf Α ◽  
Group A ◽  
Histomorphological Changes ◽  
Group B ◽  
Group D

Abstract Objective: to observe the effects of moxibustion on histomorphological changes of gastric mucosa, as well as on serum IL-6、IL-8、TNF-α，Hp IgG、CD3+、CD4+、CD8+ in helicobacter pylori (Hp) infected rats, so that to better understand how the moxibustion repairs the Hp- induced gastric mucosal injury. Methods: 40 SD rats were randomly assigned to four groups: group A (blank control), group B (Hp infection model), group C (moxibustion plus model), group D (electro-acupuncture plus model), 10 for each group. The “NaHCO3 plus Indometacin and Hp intragastric administration” method was employed to make gastritis model. Acupoints selected for “repair” purpose were Zu San Li (ST36), Zhong Wan (CV12), Guan Yuan (RN4), Pi Shu (BL20), Wei Shu(BL21). The histomorphological changes of gastric mucosa in rats were observed under light microscope after HE stain; IL-6, IL-8, TNF-α, Hp IgG values were evaluated by ELISA method; values of CD3+、CD4+、CD8+、CD4+/CD8+ were measured by flow cytometry method. Results: compared with group A, the values of IL-6, IL-8, TNF-α, Hp IgG and CD8+ in group B were increased（P＜0.01）, whereas the values of CD3+、CD4+、CD4+/CD8+ were decreased（P＜0.01）. Compared with group B, the values of IL-8（P＜0.05）,TNF-α（P＜0.05）, IL-6（P＜0.01）, Hp IgG（P＜0.01） and CD8+ （P＜0.05） in group C were decreased, whereas the values of CD3+（P＜0.05）,CD4+（P＜0.05）,CD4+/CD8+ （P＜0.05） were increased, meanwhile such values in group D had no significant changes. Compared with group D, the values of IL-6（P＜0.05）,IL-8 （P＜0.05）and Hp IgG （P＜0.01）in group C were decreased, whereas CD4+/CD8+（P＜0.05）were increased, all those changes had statistical significance. Conclusion: the preventive and therapeutic effects of moxibustion on Hp related gastritis might be realized by two ways- to inhibit the secretion of proinflammatory cytokines such as IL-6, IL-8 and TNF-α, or to regulate the production of immune factors (such as up-regulation of CD3+, CD4+ and down-regulation of CD8+).

scholarly journals EFEK PEMBERIAN ANABOLIK ANDROGENIK STEROID INJEKSI DOSIS RENDAH DAN TINGGI TERHADAP GAMBARAN HISTOPATOLOGI HATI DAN OTOT RANGKA TIKUS WISTAR (Rattus Novergicus)

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Pamela K. Sari ◽  
Poppy M. Lintong ◽  
Lily L. Loho
Keyword(s):  
Protein Synthesis ◽  
Wistar Rats ◽  
Low Dose ◽  
Anabolic Steroids ◽  
Inflammatory Cells ◽  
High Dose ◽  
Group A ◽  
Androgenic Anabolic Steroids ◽  
Group B ◽  
Group D

Abstract: Androgenic-anabolic steroids (AAS) are synthetic derivatives of the male hormone endogenous testosterone that stimulates anabolic (protein synthesis) and androgenic effects (masculinization). Long-term usage of AAS can result in liver damage. However, physiological concentrations of testosterone can stimulate protein synthesis which lead to an increase in muscle size, body mass, and endurance. This study aimed to determine the histopathology of liver and skeletal muscles of wistar rats that were given low dose and high dose injection of AAS. Subjects were 21 wistar rats divided into 7 groups. Group A was given standard pellets for 56 days (negative control), terminated on days 29,43, and 57. Group B was treated with low-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group C was treated with low-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group D was treated with low-dose AAS injection and standard pellets for 56 days, terminated on day 57. Group E was treated with high-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group F was treated with high-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group G was treated with high-dose AAS injection and standard pellets for 56 days, terminated on day 57. The results showed that the histopathology of liver and muscles in group A was still normal. In group B, the architecture of liver was still normal with a few inflammatory cells around the Kiernan triangle while in muscle the ratio of myofibril diameter was 1.28:1. In group C and group D, there were widening of the hepatic artery, bile duct, and portal vein containing blood fibrin, and inflammatory cells around the Kiernan triangle. The ratio of myofibril diameter was 1.43:1 in group C and 2.14:1 in group D. In group E, F and G, there were micro-vesicular fatty cells in the peripheral part of the liver meanwhile the myofibril diameter ratio of the muscles in group E was 1.43:1, group F 2.1:1, and group G 2.28:1. Conclusion: Administration of AAS injection of low dose and high dose for less than 4 weeks could result in inflammation, dilation of the portal vein, hepatic artery and bile duct meanwhile administration of AAS for over 4 weeks could ressult in focal fatty liver (steatosis). The administration of AAS injection of low dose and high dose for 4,6 and 8 weeks reslutid in enlargement of skeletal muscle (muscle hypertrophy).Keywords: androgenic-anabolic steroids, liver, skeletal muscleAbstrak: Anabolik Androgenik Steroid (AAS) adalah derivat sintetis dari hormon sex testosteron endogen pria, yang merangsang efek anabolik (sintesis protein) dan androgenik (maskulinisasi). Penggunaan AAS jangka panjang dapat mengakibatkan terjadinya kerusakan hati namun secara fisiologi testosteron dapat menstimulasi sintesis protein sehinggaberdampak pada peningkatan ukuran otot, massa tubuh dan ketahanan tubuh. Penelitian ini bertujuan untuk mengetahui gambaran histopatologi hati dan otot rangka wistar yang diberikan AAS injeksi dosis rendah dan dosis tinggi. Subjek penelitian 21 ekor wistar yang dibagi menjadi 7 kelompok. Kelompok A diberi pelet standar selama 56 hari (kontrol negatif), terminasi pada hari ke-29, 43, dan 57. Kelompok B diberi perlakuan AAS injeksi dosis rendah dan pelet standar selama 28 hari, terminasi hari ke-29. Kelompok C diberi AAS injeksi dosis rendah dan pelet standar selama 42 hari, terminasi hari ke-43. Kelompok D diberi AAS injeksi dosis rendah dan pelet standar selama 56 hari, terminasi hari ke-57. Kelompok E diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 28 hari, terminasi hari ke-29. Kelompok F diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 42 hari, terminasi hari ke-43. Kelompok G diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 56 hari, terminasi hari ke-57. Hasil penelitian menunjukkan pada kelompok A didapatkan gambaran histopatologi hati normal sedangkan pada otot tidak terdapat perubahan. Pada kelompok B didapatkan arsitektur hati masih normal dengan sedikit sel radang disekitar segitiga Kiernan sedangkan pada otot terlihat diameter miofibril ratio 1,28:1. Pada kelompok C dan D terlihat pelebaran arteri hepatika, duktus biliaris, dan vena porta yang berisi fibrin darah, serta sel-sel radang di sekitar segitiga Kiernan. Pada kelompok C diameter miofibril ratio 1,43;1 dan pada kelompok D 2,14:1. Pada kelompok E, F dan G terdapat sel-sel perlemakan mikrovesikuler di perifer sedangkan pada otot diameter miofibril ratio kelompok E 1,43:1, kelompok F 2,1:1, dan kelompok G 2,28:1. Simpulan: Pada pemberian AAS injeksi dosis rendah dan dosis tinggi kurang dari 4 minggu terjadi peradangan hati, pelebaran vena porta, arteri hepatika dan duktus biliaris sedangkan lebih dari 4 minggu terdapat perlemakan (steatosis) fokal hati. Pemberian AAS injeksi dosis rendah dan tinggi dalam waktu 4,6 dan 8 minggu menunjukkan pembesaran otot rangka (hipertrofi otot).Kata kunci: AAS, hati, otot rangka

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