scholarly journals Prédominance du papillomavirus humain 56 dans une sous-population de femmes sexuellement actives à Garango, Centre-Est, Burkina Faso.

2020 ◽  
Vol 150 ◽  
pp. 15499-15509
Author(s):  
Rogomenoma Alice Ouedraogo ◽  
Théodora Mahoukèdè Zohoncon ◽  
Abdoul Karim Ouattara ◽  
Jacques Simpore
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Burkina Faso ◽  
Real Time ◽  
Sexually Active ◽  
Temps Réel ◽  
Papillomavirus Humain ◽  
Hpv Genotypes ◽  
Temps Reel ◽  
Hpv 18

Objectif : cette étude a été conduite dans le but d'identifier les génotypes de Papillomavirus Humains à haut risque oncogène (HPV-HR) circulant chez les femmes sexuellement actives à Garango, au Burkina Faso. Méthodologie et résultats : avant le dépistage des lésions précancéreuses, des échantillons endocervicaux ont été prélevés chez 135 femmes sexuellement actives à Garango. L'ADN extrait a permis de caractériser 14 génotypes de HPV-HR à travers une PCR multiplexe en temps réel. Quarante-trois pourcent (43%) des femmes portaient une infection à HPV à haut risque oncogène soit 58/135. Sur les quatorze génotypes testés, treize ont été identifiés et le génotype le plus fréquent était le HPV 56 (62,5 %) suivi des HPV 18 (5,5 %), HPV 68 (4,2 %), HPV 66 (4,2 %), HPV 59 (4,2 %), HPV 58 (4,2 %), HPV 35 (4,2 %). Le HPV 33 inclus dans le vaccin anti-HPV n’a pas été identifié chez les femmes de notre étude. Conclusion et application des résultats : Ce type d'étude qui est la première à Garango a montré une forte prévalence du génotype HPV 56 qui n'est pas encore couvert par un vaccin. Ces résultats constituent une contribution scientifique sur l'épidémiologie et la distribution des génotypes HPV-HR et permettront de guider nos politiques de santé vers une meilleure prévention du cancer du col de l'utérus. Mots-clés : Papillomavirus humain à haut risque, PCR en temps réel, génotypes, femmes, Garango. Predominance of Human Papillomavirus 56 in a subpopulation of sexually active women in Garango, Central-East, Burkina Faso ABSTRACT Objective: The aim of this study was to identify circulating strains of HR-HPV among sexually active women in Garango, Burkina Faso. Methodology and results: Before screening for precancerous lesions, endocervical samples were taken from 135 sexually active women in Garango. The extracted DNA made it possible to characterize 14 HR- HPV genotypes through a real-time multiplex PCR. Forty three percent (58/135) of women had a high-risk oncogenic HPV infection. Of the fourteen genotypes tested, thirteen were identified and the most frequent genotype was HPV 56 (62.5 %) followed by HPV 18 (5.5 %), HPV 68 (4.2 %), HPV 66 (4.2 %), HPV 59 (4.2 %), HPV 58 (4.2 %), HPV35 (4.2 %). The genotype HPV 33 included in the HPV vaccine was not identified in the women in our study. Conclusion and application of finding: this type of study, which is the first one in Garango, has showed a high prevalence of genotype HPV 56 which is not yet covered by a vaccine. These results constitute a scientific contribution to the epidemiology and distribution of the HR-HPV genotypes and will help guide our health policies towards better prevention of cervical cancer. Keywords: High-Risk Human Papillomavirus, real time PCR, genotypes, women, Garango

Establishment of specifcations of the multiplex real-time PCR kit (NIHE-RealtimePCR hrHPV) for the detection of high-risk HPV genotypes

2021 ◽  
Vol 31 (4) ◽  
pp. 28-36
Author(s):  
Chu Thi Ngoc Mai ◽  
Nguyen Van Chien ◽  
Luu Thi Dung ◽  
Tran Thi Nguyet Lan ◽  
Le Thi Khanh Ly ◽  
...  
Keyword(s):  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
The Other ◽  
World Health ◽  
Real Time Systems ◽  
Hpv 16 ◽  
Hpv Genotypes ◽  
Time Systems ◽  
Hpv 18

High-risk human papillomavirus (HPV) genotypes are the leading causes of anogenital cancer, including cervical cancer. Screening these genotypes will potentially alleviate the disease burden that HPV causes. Therefore, NIHERealtime PCR hrHPV was developed based on the principle of multiplex real-time PCR. The objective of this study is to establish the specifcations of this assay in detecting and identifying 14 high-risk genotypes of HPV. The specifcations of this assay including cross-reactivity, sensitivity and specifcity, the limit of detection and stability, were evaluated with clinical samples, HPV positive control code 06/202 and 06/206 (National Institute for Biological Standards and Control) and in-house plasmids. Four different real-time systems and two commercial assays were applied for comparison. No cross detection of bacteria and viruses related to other genital-anal was observed. The sensitivity and the specifcity of this kit both reached 100% for HPV-18, 92.3% and 100% for HPV-16, and ranged from 90.0% - 100% for the other 12 high-risk genotypes (n = 92) with the detection limit of 5 copies/µl for HPV-16 and HPV-18, and 10 copies/µl for the other 12 high-risk genotypes HPV. This test was stable for up to 12 months under -20oC condition. Equivalent performance was demonstrated on all four real-time systems. Finally, the product was highly comparable with the Sacace kit, Italy (HPV Genotypes 14 Real-™ Quant, V67-100FRT), evaluated by the World Health Organization. The NIHE kit has the potential to be used in the early detection of HPV infection to improve control, prevention and treatment of HPV-related anogenital diseases.

scholarly journals Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Akouélé P. Kuassi-Kpede ◽  
Essolakina Dolou ◽  
Théodora M. Zohoncon ◽  
Ina Marie Angèle Traore ◽  
Gnatoulma Katawa ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
West Africa ◽  
Public Health Problem ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Genotypes ◽  
Precancerous Cervical Lesions ◽  
High Risk Hpv ◽  
First Time

Abstract Background The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. Methods Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. Results Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. Conclusion This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

scholarly journals Genotypic distribution of human oncogenic papillomaviruses in sexually active women in Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions

2020 ◽  
Vol 11 (1) ◽  
pp. 125-136
Author(s):  
Rogomenoma Alice Ouedraogo ◽  
Théodora Mahoukèdè Zohoncon ◽  
Ina Marie Angèle Traore ◽  
Abdoul Karim Ouattara ◽  
Sindimalgdé Patricia Guigma ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Large Scale ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Sexually Active ◽  
Cervical Lesions ◽  
Cross Sectional ◽  
Genotypic Distribution ◽  
Hpv Genotypes ◽  
Central Eastern

AbstractObjectivethis study was conducted to determine the distribution of high-risk human papillomavirus (HR-HPV) genotypes in women in the general population of three regions of Burkina Faso.MethodThis multicenter, descriptive cross-sectional study involved 1321 sexually active women in five cities in three regions of Burkina Faso: Central, Central-Eastern and Hauts-Bassins regions. After collection of endocervical specimens, pre-cervical lesions were screened by visual inspection with acetic acid and lugol (VIA / VILI). HR-HPV genotypes were characterized by multiplex real-time PCR after extraction of viral DNA.ResultsThe mean age of women was 31.98 ± 10.09 years. The HR-HPV infection in the three regions ranged from 26.16% to 43.26% with 35.42% as overall prevalence in women. The most common HR-HPV genotypes in descending order were: HPV 56, 52, 66, 59, 39, 51, 18, 35. The prevalence of bivalent vaccine genotypes (HPV16 / 18) was 7.83% against 63.78% of genotypes not covered by HPV vaccine; 36.32% (170/468) of women had multiple concomitant HR-HPV infections.Conclusionthis study showed significant regional variation and high prevalence of HR-HPV infection in women. The predominant genotypes differ from those covered by available vaccines in Burkina Faso. These results will help guide our health policies towards better prevention of cervical cancer. The diversity of oncogenic genotypes is sparking a large-scale study in the West African sub-region, particularly in cases of cancer and the introduction of the nonavalent vaccine which includes HPV 52 found among the predominant genotypes in this study.

scholarly journals Prevalence of precancerous cervical lesions and high-risk human papillomavirus types in Yaounde, Cameroon

2021 ◽  
Vol 15 (09) ◽  
pp. 1339-1345
Author(s):  
Richard Tagne Simo ◽  
Arsène G Djoko Nono ◽  
Hervet Paulin Fogang Dongmo ◽  
Paul F Seke Etet ◽  
Bertrand Kiafon Fonyuy ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Precancerous Lesions ◽  
Health Centre ◽  
Oral Contraceptive Pill ◽  
Cervical Lesions ◽  
Hpv 16 ◽  
Hpv Types ◽  
Cytologic Abnormalities ◽  
Hpv 18

Introduction: Various Human papillomavirus (HPV) types cause cervical cancer, and represent the primary cause of cancer death in Africa and the second cause of most common cancers in Cameroon. Herein, we determined the prevalence of high-risk HPV types in women and associated cervical cytologic abnormalities in Yaounde, Cameroon. Methodology: A cross-sectional study targeting HPV-positive women aged 20 and over was conducted between March and June 2020 at the Saint Martin de Porres’ Health Centre in Yaounde. HPV tests were performed by PCR for detection of HPVs 16, 18, 33, and 45. The test was performed on 616 women using exfoliated cell specimens; then, we processed on cytological diagnosis with Pap smears on HPV positive specimens. Results: The HPV types tested were detected in 137 participants, of which 38.7% with multiple HPV infections, and the remaining part with single HPV infections of type HPV 16 (28.5%), HPV 18 (17.5%), HPV 33 (10.2%), and HPV 45 (5.1%). Cervical cytologic abnormalities were found in 69.34% of participants including: LSIL (49.63%), HSIL (15.32%), ASC-US (3.66%) and AGC (0.73%). Co-infections with HPV 16 and HPV 18 were significantly associated with HSIL (p = 0.001) lesions, while HPV 45 was more common in participants with normal cytology (p = 0.001). Cervical lesion occurrence was significantly associated with the number of sexual partners (p = 0.02) and history of oral contraceptive pill use (p = 0.001). Conclusions: Our results suggest that HPV 16 and 18 are predominant in Yaounde, and are associated with more severe precancerous lesions.

Carriage of high-risk human papillomavirus infection in male subjects in Ouagadougou, Burkina Faso

2019 ◽  
Author(s):  
Sya Forazoun Bienvenue Tamini ◽  
Theodora Mahoukèdè Zohoncon ◽  
Serge Theophile Soubeiga ◽  
Florencia Wendkuuni Djigma ◽  
Ina Marie Angele Traore ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Burkina Faso ◽  
Public Health Problem ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Multiple Infections ◽  
Cross Sectional ◽  
High Risk Hpv ◽  
Male Subjects

Abstract Background Human papillomavirus (HPV) infection is a public health problem in Africa because of its frequency and its various consequences in both men and women. In Burkina Faso, there is little data on the carriage of HPV infection in male subjects. This study aimed to determine the carriage of HPV infection in male subjects in Ouagadougou, Burkina Faso.Methods This was a cross-sectional study conducted from December 2015 to September 2016. During this study period, a total of 124 male subjects who gave informed consent were recruited as part of a fertility assessment. Semen samples were analyzed by Real- Time PCR using Real TM Quant High Risk Screen HPV kit to detect 14 high-risk HPV genotypes. The Chi square test was used for comparisons.Results Of the 124 male subjects, 22 were positive for at least one high-risk HPV, with a prevalence of 17.7%. HPV 56 was the most represented genotype with a frequency of 20%, followed by HPV 39 (11%) and HPV 68 (11%), HPV 16 (8%). The genotypes HPV 18, HPV33, HPV35, HPV51 and HPV52 also had the same frequency of 6% each and the HPV 31, 45, 59 and 66 had the same frequency of 3% each. Of the 22 positive cases, 10 were multiple infections. Spermocytogram of patients showed at least one abnormality in 87.9% of cases. Necrozoospermia and oligozoospermia were the most abnormalities observed with 42% and 40% respectively followed by asthenospermia (18%); teratozoospermia (16%) and azoospermia (14%).Conclusion This study showed that like women, men are infected by HPV with a relatively high prevalence. In addition, the predominant genotypes in the male subjects of this study are not those targeted by the HPV vaccines available in our countries. Hence the need to reinforce the strategies for controlling HPV infection and HPV prophylactic vaccination for young boys.

Comparison of Human Papillomavirus Genotypes in Penile Intraepithelial Neoplasia and Associated Lesions: LCM-PCR Study of 87 Lesions in 8 Patients

2019 ◽  
Vol 28 (3) ◽  
pp. 265-272 ◽  
Author(s):  
María José Fernández-Nestosa ◽  
Nuria Guimerà ◽  
Diego F. Sanchez ◽  
Sofía Cañete-Portillo ◽  
Antonella Lobatti ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Precancerous Lesions ◽  
Intraepithelial Neoplasia ◽  
Associated Lesions ◽  
Hpv Genotypes ◽  
Flat Lesions ◽  
High Risk Hpv ◽  
Intraepithelial Lesions ◽  
Penile Intraepithelial Neoplasia

Penile intraepithelial neoplasia (PeIN) is currently classified in human papillomavirus (HPV)- and non-HPV-related subtypes with variable HPV genotypes. PeINs are frequently associated with other intraepithelial lesions in the same specimen. The aim of this study was to detect and compare HPV genotypes in PeINs and associated lesions using high-precision laser capture microdissection-polymerase chain reaction and p16INK4a immunostaining. We evaluated resected penile specimens from 8 patients and identified 33 PeINs and 54 associated lesions. The most common subtype was warty PeIN, followed by warty-basaloid and basaloid PeIN. Associated lesions were classical condylomas (17 cases), atypical classical condylomas (2 cases), flat condylomas (9 cases), atypical flat condylomas (6 cases), flat lesions with mild atypia (12 cases), and squamous hyperplasia (8 cases). After a comparison, identical HPV genotypes were found in PeIN and associated lesions in the majority of the patients (7 of 8 patients). HPV16 was the most common genotype present in both PeIN and corresponding associated lesion (50% of the patients). Nonspecific flat lesions with mild atypia, classical condylomas, and atypical condylomas were the type of associated lesions most commonly related to HPV16. Other high-risk HPV genotypes present in PeIN and associated nonspecific flat lesion with mild atypia were HPV35 and HPV39. In this study of HPV in the microenvironment of penile precancerous lesions, we identified identical high-risk HPV genotypes in PeIN and classical, flat, or atypical condylomas and, specially, in nonspecific flat lesions with mild atypia. It is possible that some of these lesions represent hitherto unrecognized precancerous lesions.

scholarly journals Clinical Validation of the HPV-Risk Assay, a Novel Real-Time PCR Assay for Detection of High-Risk Human Papillomavirus DNA by Targeting the E7 Region

2014 ◽  
Vol 52 (3) ◽  
pp. 890-896 ◽  
Author(s):  
A. T. Hesselink ◽  
J. Berkhof ◽  
M. L. van der Salm ◽  
A. P. van Splunter ◽  
T. H. Geelen ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Validation ◽  
Pcr Assay ◽  
Papillomavirus Dna
Sign in / Sign up

Export Citation Format

Share Document