scholarly journals High Throughput Sequencing Detect Gingivitis And Periodontal Oral Bacteria In Alzheimer’s Disease Autopsy Brains

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Huma Siddiqui ◽  
Emenike RK Eribe ◽  
Sim K Singhrao ◽  
Ingar Olsen
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Oral Bacteria

scholarly journals Plasma Amyloid Concentration in Alzheimer’s Disease: Performance of a High-Throughput Amyloid Assay in Distinguishing Alzheimer’s Disease Cases from Controls

2020 ◽  
Vol 74 (4) ◽  
pp. 1285-1294 ◽  
Author(s):  
Insa Feinkohl ◽  
Carola G. Schipke ◽  
Jochen Kruppa ◽  
Felix Menne ◽  
Georg Winterer ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Throughput

[P3-245]: ANALYSIS OF THE HUMORAL RESPONSE IN ALZHEIMER's DISEASE USING THE HIGH-THROUGHPUT SCREENING COMBINATION OF T7 PHAGE LIBRARIES AND PROTEIN MICROARRAYS

2017 ◽  
Vol 13 (7S_Part_21) ◽  
pp. P1034-P1034
Author(s):  
Pablo San Segundo-Acosta ◽  
Ana Montero-Calle ◽  
Maria Garranzo-Asensio ◽  
Carmen Oeo-Santos ◽  
Juan Carlos López-Rodríguez ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Throughput ◽  
High Throughput Screening ◽  
Humoral Response ◽  
Protein Microarrays ◽  
Phage Libraries ◽  
T7 Phage

scholarly journals The MUC6/AP2A2 Locus and Its Relevance to Alzheimer’s Disease: A Review

2020 ◽  
Vol 79 (6) ◽  
pp. 568-584 ◽  
Author(s):  
Peter T Nelson ◽  
David W Fardo ◽  
Yuriko Katsumata
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Protein Complex ◽  
High Throughput Sequencing ◽  
Relevant Literature ◽  
Adaptor Protein ◽  
Variable Number ◽  
Repeat Expansion ◽  
Background Information ◽  
Coated Vesicle

Abstract We recently reported evidence of Alzheimer’s disease (AD)-linked genetic variation within the mucin 6 (MUC6) gene on chromosome 11p, nearby the adaptor-related protein complex 2 subunit alpha 2 (AP2A2) gene. This locus has interesting features related to human genomics and clinical research. MUC6 gene variants have been reported to potentially influence viral—including herpesvirus—immunity and the gut microbiome. Within the MUC6 gene is a unique variable number of tandem repeat (VNTR) region. We discovered an association between MUC6 VNTR repeat expansion and AD pathologic severity, particularly tau proteinopathy. Here, we review the relevant literature. The AD-linked VNTR polymorphism may also influence AP2A2 gene expression. AP2A2 encodes a polypeptide component of the adaptor protein complex, AP-2, which is involved in clathrin-coated vesicle function and was previously implicated in AD pathogenesis. To provide background information, we describe some key knowledge gaps in AD genetics research. The “missing/hidden heritability problem” of AD is highlighted. Extensive portions of the human genome, including the MUC6 VNTR, have not been thoroughly evaluated due to limitations of existing high-throughput sequencing technology. We present and discuss additional data, along with cautionary considerations, relevant to the hypothesis that MUC6 repeat expansion influences AD pathogenesis.

scholarly journals P1-130: HIGH-THROUGHPUT OMICS EXPLORER FOR ALZHEIMER'S DISEASE AND COMPLEX TRAITS GENETIC ANALYSIS

2019 ◽  
Vol 15 ◽  
pp. P285-P286
Author(s):  
Jorge Alberto Bahena ◽  
Fabiana H.G. Farias ◽  
Kathie A. Mihindukulasuriya ◽  
John P. Budde ◽  
Carlos Cruchaga ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Genetic Analysis ◽  
High Throughput ◽  
Complex Traits

scholarly journals High-throughput epitope profiling of antibodies in the plasma of Alzheimer’s disease patients using random peptide microarrays

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kyu-Young Sim ◽  
Sang-Heon Park ◽  
Kyu Yeong Choi ◽  
Jung Eun Park ◽  
Jung Sup Lee ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Throughput ◽  
Random Peptide ◽  
Peptide Microarrays

An integrated strategy for rapid screening of multi-target lead compounds for the treatment of Alzheimer's disease from traditional Chinese medicines by UHPLC combined with high-throughput screening: A case study on Salviae Miltiorrhizae Radix et Rhizoma

2021 ◽  
Vol 17 ◽  
Author(s):  
Minmin Zhang ◽  
Siduo Zhou ◽  
Wei Liu ◽  
Huijiao Yan ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Throughput ◽  
Inhibitory Activity ◽  
High Throughput Screening ◽  
Hplc Analysis ◽  
Salviae Miltiorrhizae ◽  
Salviae Miltiorrhizae Radix ◽  
Ache Inhibitory Activity

Background: Salviae Miltiorrhizae Radix et Rhizoma (Red Sage root) is widely used in traditional Chinese medicine (TCM) for the treatment of Alzheimer’s disease (AD) with demonstrated curative effects, based on the concept of "one drug with multiple therapeutic targets," which appears to be a good strategy for AD treatment. Objective: This study aimed to develop of high-throughput screening (HTS) method for multi-therapeutic target components found in complex TCMs, which are active against AD, using Red Sage root as the case study. Method: Acetylcholinesterase (AChE) inhibitors (AChEIs) from Red Sage root extracts were pre-screened by ultrafiltration-HPLC (UF-HPLC) analysis, in which AChE was added to the extract and then ultrafiltered to remove non-binding compounds. Potential AChEIs were identified by HPLC analysis of compounds bound to AChE. A microplate-based HTS was then used to quantify the AChE inhibitory activity and antioxidant activity of the pre-screened compounds. Results: Pre-screening found ten potential inhibitors, which were identified by ESI-TOF/MS; six of these were purified by semi-preparative HPLC: Oleoyl neocryptotanshinone (1), Dihydrotanshinone Ⅰ (2), Cryptotanshinone (3), Tanshinone Ⅰ (4), Tanshinone ⅡA (5) and Miltirone (6). All six compounds had good AChE inhibitory activity and weak DPPH scavenging capacity. Conclusion: This study provides a platform and technology support for the rapid discovery of multi-target components, potentially active against AD, from complex TCMs and with strong potential for adaptation to the discovery of treatments for other diseases.

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