scholarly journals Regulation of odontoblast differentiation by Cpne7, a dental epithelium-derived factor, via the Runx2–Nfic–Osx–Dspp pathway

2017 ◽  
Vol 38 (1) ◽  
pp. 55-66
Author(s):  
Hojae Choi ◽  
박주철
Keyword(s):  
Odontoblast Differentiation ◽  
Dental Epithelium

Wnt Production in Dental Epithelium Is Crucial for Tooth Differentiation

2019 ◽  
Vol 98 (5) ◽  
pp. 580-588 ◽  
Author(s):  
Y. Xiong ◽  
Y. Fang ◽  
Y. Qian ◽  
Y. Liu ◽  
X. Yang ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Signaling Pathway ◽  
Tooth Development ◽  
Wnt Signaling Pathway ◽  
Conditional Knockout ◽  
Canonical Wnt Signaling ◽  
Odontoblast Differentiation ◽  
Wnt Ligands ◽  
Dental Epithelium ◽  
Canonical Wnt

The Wnt ligands display varied spatiotemporal expression in the epithelium and mesenchyme in the developing tooth. Thus far, the actions of these differentially expressed Wnt ligands on tooth development are not clear. Shh expression specifies the odontogenic epithelium during initiation and is consistently restricted to the dental epithelium during tooth development. In this study, we inactivate Wntless ( Wls), the key regulator for Wnt trafficking, by Shh-Cre to investigate how the Wnt ligands produced in the dental epithelium lineage act on tooth development. We find that conditional knockout of Wls by Shh-Cre leads to defective ameloblast and odontoblast differentiation. WlsShh-Cre teeth display reduced canonical Wnt signaling activity in the inner enamel epithelium and the underlying mesenchyme at the early bell stage, as exhibited by target gene expression and BAT-gal staining. The expression of Wnt5a and Wnt10b is not changed in WlsShh-Cre teeth. By contrast, Wnt10a expression is significantly increased in response to epithelial Wls deficiency. In addition, the expression of Hedgehog signaling pathway components Shh, Gli1, and Patched1 was greatly decreased in WlsShh-Cre teeth. Epithelial Wls loss of function in Shh lineage also leads to aberrant cell proliferation in dental epithelium and mesenchyme at embryonic day 16.5; however, the cell apoptosis is unaffected. Moreover, we find that Decorin and Col1a1, the key markers for odontoblast differentiation that are downregulated in WlsShh-Cre teeth, act as direct downstream targets of the canonical Wnt signaling pathway by chromatin immunoprecipitation analysis. Additionally, Decorin and Col1a1 expression can be increased by lithium chloride (LiCl) treatment in the in vitro tooth explants. Taken together, our results suggest that the spatial expression of Wnt ligands within the dental epithelial lineage regulates the differentiation of tooth structures in later stages.

Enamel Knots as Signaling Centers Linking Tooth Morphogenesis and Odontoblast Differentiation

2001 ◽  
Vol 15 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Irma Thesleff ◽  
Soile Keranen ◽  
Jukka Jernvall
Keyword(s):  
Tooth Development ◽  
Experimental Studies ◽  
Cell Lineage ◽  
Signaling Molecules ◽  
Enamel Knot ◽  
Odontoblast Differentiation ◽  
Dental Papilla ◽  
Dental Epithelium ◽  
Tooth Morphogenesis ◽  
Enamel Knots

Odontoblasts differentiate from the cells of the dental papilla, and it has been well-established that their differentiation in developing teeth is induced by the dental epithelium. In experimental studies, no other mesenchymal cells have been shown to have the capacity to differentiate into odontoblasts, indicating that the dental papilla cells have been committed to odontoblast cell lineage during earlier developmental stages. We propose that the advancing differentiation within the odontoblast cell lineage is regulated by sequential epithelial signals. The first epithelial signals from the early oral ectoderm induce the odontogenic potential in the cranial neural crest cells. The next step in the determination of the odontogenic cell lineage is the development of the dental papilla from odontogenic mesenchyme. The formation of the dental papilla starts at the onset of the transition from the bud to the cap stage of tooth morphogenesis, and this is regulated by epithelial signals from the primary enamel knot. The primary enamel knot is a signaling center which forms at the tip of the epithelial tooth bud. It becomes fully developed and morphologically discernible in the cap-stage dental epithelium and expresses at least ten different signaling molecules belonging to the BMP, FGF, Hh, and Wnt families. In molar teeth, secondary enamel knots appear in the enamel epithelium at the sites of the future cusps. They also express several signaling molecules, and their formation precedes the folding and growth of the epithelium. The differentiation of odontoblasts always starts from the tips of the cusps, and therefore, it is conceivable that some of the signals expressed in the enamel knots may act as inducers of odontoblast differentiation. The functions of the different signals in enamel knots are not precisely known. We have shown that FGFs stimulate the proliferation of mesenchymal as well as epithelial cells, and they may also regulate the growth of the cusps. We have proposed that the enamel knot signals also have important roles, together with mesenchymal signals, in regulating the patterning of the cusps and hence the shape of the tooth crown. We suggest that the enamel knots are central regulators of tooth development, since they link cell differentiation to morphogenesis.

FGFs-1 and -2, and TGFβ 1 as Inductive Signals Modulating in vitro Odontoblast Differentiation

2001 ◽  
Vol 15 (1) ◽  
pp. 34-38 ◽  
Author(s):  
F.J. Unda ◽  
A. Martín ◽  
C. Hernandez ◽  
G. Pérez-Nanclares ◽  
E. Hilario ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Terminal Differentiation ◽  
Induce Polarization ◽  
Functional Differentiation ◽  
Cell Polarization ◽  
Odontoblast Differentiation ◽  
Exogenous Addition ◽  
Dental Epithelium ◽  
Extracellular Matrix Secretion

We have studied the expression of FGF1 and FGF2 during mouse odontogenesis by immunohistochemistry. FGF1 was detected in differentiated odontoblasts and at the secretory pole of ameloblasts. Localization of FGF2 was mainly observed within the basement membrane interposed between dental epithelium and dental mesenchyme. These findings indicate that FGF1 and FGF2 may participate in the control of odontoblast and ameloblast differentiation. Thereafter, we studied the ability of FGF1 and FGF2, alone or in combination with TGFβ1, to induce polarization and/or functional differentiation of pre-odontoblasts. Dental papillae (DP) obtained from first lower molars of 17-day-old mouse embryo were cultured in the presence or the absence of growth factors. DP cultured with FGFl+TGFβ1 showed gradients of odontoblast-like cell differentiation, which displayed alkaline phosphatase reactivity. DP treated with FGF2+TGFβ1 exhibited pre-odontoblast cell polarization, and the cell bodies displayed long cytoplasm processes. However, following this treatment we did not observe extracellular matrix secretion, and alkaline phosphatase activity was completely inhibited. In summary, our results show that exogenous addition of FGF1 to pre-odontoblasts induces their terminal differentiation, by synergistically acting with TGFβ1. In contrast, FGF2 may regulate the effect of TGFβ1, permitting cell polarization but restraining pre-odontoblast functions.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

scholarly journals Timing of Mouse Molar Formation Is Independent of Jaw Length Including Retromolar Space

2021 ◽  
Vol 9 (1) ◽  
pp. 8
Author(s):  
Daisy (Jihyung) Ko ◽  
Tess Kelly ◽  
Lacey Thompson ◽  
Jasmene K. Uppal ◽  
Nasim Rostampour ◽  
...  
Keyword(s):  
Onset Time ◽  
Ct Scanning ◽  
Micro Ct ◽  
Third Molars ◽  
Developmental Duration ◽  
Dental Lamina ◽  
Permanent Molars ◽  
Dental Epithelium ◽  
Space Conditions

For humans and other mammals to eat effectively, teeth must develop properly inside the jaw. Deciphering craniodental integration is central to explaining the timely formation of permanent molars, including third molars which are often impacted in humans, and to clarifying how teeth and jaws fit, function and evolve together. A factor long-posited to influence molar onset time is the jaw space available for each molar organ to form within. Here, we tested whether each successive molar initiates only after a minimum threshold of space is created via jaw growth. We used synchrotron-based micro-CT scanning to assess developing molars in situ within jaws of C57BL/6J mice aged E10 to P32, encompassing molar onset to emergence. We compared total jaw, retromolar and molar lengths, and molar onset times, between upper and lower jaws. Initiation time and developmental duration were comparable between molar upper and lower counterparts despite shorter, slower-growing retromolar space in the upper jaw, and despite size differences between upper and lower molars. Timing of molar formation appears unmoved by jaw length including space. Conditions within the dental lamina likely influence molar onset much more than surrounding jaw tissues. We theorize that molar initiation is contingent on sufficient surface area for the physical reorganization of dental epithelium and its invagination of underlying mesenchyme.

LncRNA DANCR sponges miR-216a to inhibit odontoblast differentiation through upregulating c-Cbl

2020 ◽  
Vol 387 (1) ◽  
pp. 111751
Author(s):  
Lingling Chen ◽  
Zhi Song ◽  
Jinyan Wu ◽  
Qiting Huang ◽  
Zongshan Shen ◽  
...  
Keyword(s):  
Odontoblast Differentiation

scholarly journals BMP Activity Is Required for Tooth Development from the Lamina to Bud Stage

2012 ◽  
Vol 91 (7) ◽  
pp. 690-695 ◽  
Author(s):  
Y. Wang ◽  
L. Li ◽  
Y. Zheng ◽  
G. Yuan ◽  
G. Yang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Molecular Marker ◽  
Tooth Development ◽  
Tooth Germ ◽  
Bmp Signaling ◽  
Expression Of Genes ◽  
Dental Epithelium ◽  
Initiation Stage ◽  
Transgenic Embryos ◽  
Bmp Antagonist

Several Bmp genes are expressed in the developing mouse tooth germ from the initiation to the late-differentiation stages, and play pivotal roles in multiple steps of tooth development. In this study, we investigated the requirement of BMP activity in early tooth development by transgenic overexpression of the extracellular BMP antagonist Noggin. We show that overexpression of Noggin in the dental epithelium at the tooth initiation stage arrests tooth development at the lamina/early-bud stage. This phenotype is coupled with a significantly reduced level of cell proliferation rate and a down-regulation of Cyclin-D1 expression, specifically in the dental epithelium. Despite unaltered expression of genes known to be implicated in early tooth development in the dental mesenchyme and dental epithelium of transgenic embryos, the expression of Pitx2, a molecular marker for the dental epithelium, became down-regulated, suggesting the loss of odontogenic fate in the transgenic dental epithelium. Our results reveal a novel role for BMP signaling in the progression of tooth development from the lamina stage to the bud stage by regulating cell proliferation and by maintaining odontogenic fate of the dental epithelium.

scholarly journals Microtubule-associated Protein 1b, a Neuronal Marker Involved in Odontoblast Differentiation

2009 ◽  
Vol 35 (7) ◽  
pp. 992-996 ◽  
Author(s):  
Jean-Christophe Maurin ◽  
Marie-Lise Couble ◽  
Marie-Jeanne Staquet ◽  
Florence Carrouel ◽  
Imad About ◽  
...  
Keyword(s):  
Neuronal Marker ◽  
Odontoblast Differentiation

Suberoylanilide Hydroxamic Acid Enhances Odontoblast Differentiation

2012 ◽  
Vol 91 (5) ◽  
pp. 506-512 ◽  
Author(s):  
A. Kwon ◽  
H.-J. Park ◽  
K. Baek ◽  
H.-L. Lee ◽  
J.-C. Park ◽  
...  
Keyword(s):  
Hydroxamic Acid ◽  
Suberoylanilide Hydroxamic Acid ◽  
Odontoblast Differentiation
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