Induction of nonspecific resistance of Scotch pine seedlings under influence of NO donor sodium nitroprusside. 2. Increase in resistance against soil drought

2018 ◽  
Vol 2018 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Yu. V. Karpets ◽  
◽  
M. A. Shklyarevskiy ◽  
G. A. Lugova ◽  
◽  
...  
Keyword(s):  
Sodium Nitroprusside ◽  
Soil Drought ◽  
Scotch Pine ◽  
Nonspecific Resistance ◽  
No Donor ◽  
Pine Seedlings

scholarly journals Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Feng-Hua Chen ◽  
Ke Li ◽  
Lu Yin ◽  
Chun-Qiu Chen ◽  
Zhao-Wen Yan ◽  
...  
Keyword(s):  
Small Intestine ◽  
Molecular Markers ◽  
Protective Effect ◽  
Sodium Nitroprusside ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Small Bowel Transplantation ◽  
Mucosal Barrier ◽  
No Donor

The intestinal mucosal epithelium is extremely susceptible to even brief periods of ischemia. Mucosal barrier damage, which is associated with ischemia/reperfusion (I/R) injury and consequently bacterial translocation, remains a major obstacle for clinically successful small bowel transplantation (SBT). Previous studies have demonstrated a protective effect of nitric oxide (NO) on other transplanted organs and NO mediated intestinal protection has also been reportedin vitro. The aim of this study was to evaluate the effect of sodium nitroprusside (SNP), NO donor, on graft mucosal histology and molecular markers of function after SBT in rats. We used SNP in different period of heterotopic SBT rats. The groups consisted of SBT, pre-SNP group, and post-SNP group. Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples. In addition, mucosal samples from the pre-SNP group showed higher Na+-K+-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples. The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine.

Nitric oxide increases fluid extravasation from the splenic circulation of the rat

2001 ◽  
Vol 280 (4) ◽  
pp. R959-R967 ◽  
Author(s):  
Peter S. Andrew ◽  
Yiming Deng ◽  
Richard Sultanian ◽  
Susan Kaufman
Keyword(s):  
Nitric Oxide ◽  
Sodium Nitroprusside ◽  
Lymphatic System ◽  
Resistance Arteries ◽  
No Donor ◽  
Fluid Extravasation ◽  
Resistance Vessels ◽  
Blood Inflow ◽  
The Difference ◽  
Arteries And Veins

We hypothesized that nitric oxide (NO) contributes to intrasplenic fluid extravasation by inducing greater relaxation in splenic resistance arteries than veins such that intrasplenic microvascular pressure (PC) rises. Fluid efflux was estimated by measuring the difference between splenic blood inflow and outflow. Intrasplenic infusion of the NO donor S-nitroso- N-acetylpenicillamine (SNAP) (0.3 μg · 10 μl−1 · min−1) caused a significant increase in intrasplenic fluid efflux (baseline: 0.8 ± 0.4 ml/min, n = 10 vs. peak rise during SNAP infusion: 1.3 ± 0.4 ml/min, n = 10; P < 0.05). Intrasplenic PC was measured in the isolated, blood-perfused rat spleen. Intrasplenic infusion of SNAP (0.1 μg · 10 μl−1 · min−1) caused a significant increase in PC (saline: 10.9 ± 0.2 mmHg, n = 3 vs. SNAP: 12.2 ± 0.2 mmHg, n = 3; P < 0.05). Vasoreactivity of preconstricted splenic resistance vessels to sodium nitroprusside (SNP) (1 × 10−12-1 × 10−4 M) and SNAP (1 × 10−10-3 × 10−4 M) was investigated with the use of a wire myograph system. Significantly greater relaxation of arterioles than of venules occurred with both SNP (%maximal vasorelaxation: artery 96 ± 2.3, n = 9 vs. vein 26 ± 1.9, n = 10) and SNAP (%maximal vasorelaxation: artery 50 ± 3.5, n = 11 vs. vein 32 ± 1.7, n = 8). These results are consistent with our proposal that differential vasoreactivity of splenic resistance arteries and veins to NO elevates intrasplenic PC and increases fluid extravasation into the systemic lymphatic system.

Airways of a hyperresponsive rat strain show decreased relaxant responses to sodium nitroprusside

1995 ◽  
Vol 269 (1) ◽  
pp. L85-L91 ◽  
Author(s):  
Y. Jia ◽  
L. Xu ◽  
S. Heisler ◽  
J. G. Martin
Keyword(s):  
Sodium Nitroprusside ◽  
Inhibitory Effect ◽  
Airway Responsiveness ◽  
No Donor ◽  
Relaxant Effect ◽  
Inhibition Concentration ◽  
Relaxant Response ◽  
Rat Strain ◽  
Guanylyl Cyclase Activity

The aim of the current studies was to investigate the possibility that a decreased relaxant response to nitric oxide (NO) might contribute to strain-related differences in airway responsiveness in the rat. Isolated tracheal rings from hyperresponsive. Fisher rats were confirmed to be more responsive to carbachol [mean effective concentration (EC50) = 2.45 x 10(-7) M] than those from Lewis (EC50 = 3.60 x 10(-7) M, P < 0.03) and ACI (EC50 = 3.85 x 10(-7) M, P < 0.01) rats. Sodium nitroprusside (SNP), a NO donor, caused relaxation of the carbachol (10(-6) M) contracted tracheal rings, but the half-maximal inhibition concentration (IC50) SNP in Fisher rats (5.60 x 10(-6) M) was significantly higher than in Lewis (1.34 x 10(-6) M, P < 0.001) and ACI rats (1.13 x 10(-6) M, P < 0.0005). The inhibitory effect of SNP on airway responsiveness to inhaled methacholine (MCh) in vivo was also less pronounced for Fisher than Lewis rats. SNP induced an accumulation of guanosine 3',5'-cyclic monophosphate (cGMP) in cultured tracheal smooth muscle cells (TSM). Fisher TSM produced less cGMP on exposure to SNP compared with TSM from ACI (P < 0.01) and Lewis (P < 0.0001) rats. A decreased guanylyl cyclase activity may account for the impaired relaxant effect of SNP in Fisher rats.

Segmental heterogeneity of NO-mediated pulmonary vasodilation in rats

1994 ◽  
Vol 267 (2) ◽  
pp. H494-H499 ◽  
Author(s):  
M. R. Eichinger ◽  
B. R. Walker
Keyword(s):  
Sodium Nitroprusside ◽  
Arginine Vasopressin ◽  
Calcium Ionophore ◽  
Vascular Occlusion ◽  
Physiological Saline ◽  
Calcium Ionophore A23187 ◽  
Pulmonary Vasculature ◽  
Ionophore A23187 ◽  
Sprague Dawley ◽  
No Donor

Nitric oxide (NO) is known to elicit vasodilation in the preconstricted rat lung. However, the sites of dilation within the pulmonary vasculature remain unknown. We hypothesized that donated NO would dilate all areas of constriction within the pulmonary vasculature, whereas receptor-mediated, NO-induced dilations would correspond to regional binding of agents. Isolated lungs from male Sprague-Dawley rats were perfused at constant flow with physiological saline solution. Pulmonary arterial and pulmonary venous pressures were monitored, while pulmonary microvascular pressures were estimated by vascular occlusion. Lungs were constricted with U-46619, and upon development of a stable degree of vasoconstriction, the NO donor sodium nitroprusside or the endothelium-dependent dilators A23187, arginine vasopressin, or ATP were administered. U-46619 caused constriction of both arterial and venous segments. Administration of sodium nitroprusside and the calcium ionophore A23187 elicited similar dilation of preconstricted arterial and venous segments. Arginine vasopressin significantly dilated both arterial and venous segments, with a greater reversal of venous resistance. In contrast, ATP significantly reduced arterial resistance more than venous. These results demonstrate that donated NO uniformly dilates all constricted regions of the pulmonary vasculature. However, receptor-mediated, endothelium-dependent dilators display characteristic heterogeneities in the sites of decreased pulmonary vascular resistance.

scholarly journals Effects of cGMP and sodium nitroprusside on odor responses in turtle olfactory sensory neurons

1998 ◽  
Vol 275 (5) ◽  
pp. C1201-C1206 ◽  
Author(s):  
Kouhei Inamura ◽  
Makoto Kashiwayanagi ◽  
Kenzo Kurihara
Keyword(s):  
Sodium Nitroprusside ◽  
Dependent Manner ◽  
High Concentration ◽  
No Donor ◽  
Inward Current ◽  
Inward Currents ◽  
Induced Currents ◽  
Dose Dependent ◽  
Camp And Cgmp ◽  
Dependent Pathway

The effects of cGMP and sodium nitroprusside (SNP) on odor responses in isolated turtle olfactory neurons were examined. The inward current induced by dialysis of a mixture of 1 mM cAMP and 1 mM cGMP was similar to that induced by dialysis of 1 mM cAMP or 1 mM cGMP alone. After the neurons were desensitized by the application of 1 mM cGMP, 3 mM 8-(4-chlorophenylthio)-cAMP, a membrane-permeable cAMP analog, did not elicit any current, indicating that both cAMP and cGMP activated the same channel. Extracellular application of SNP, a nitric oxide (NO) donor, evoked inward currents in a dose-dependent manner. However, application of SNP did not induce any currents after desensitization of the cGMP-induced currents, suggesting that SNP-induced currents are mediated via the cGMP-dependent pathway. Application of the cAMP-producing odorants to the neurons induced a large inward current even after neurons were desensitized to a high concentration of cGMP or SNP. These results suggest that the transduction pathway independent of cAMP, cGMP, and NO also contributes to the generation of odor responses in addition to the cAMP-dependent pathway.

Sodium Nitroprusside-Mediated Modulation of Growth and Antioxidant Defense in the InVitro raised Plantlets of Peanut Genotypes

2014 ◽  
Vol 41 (1) ◽  
pp. 25-31 ◽  
Author(s):  
A. Verma ◽  
C. P. Malik ◽  
V. K. Gupta
Keyword(s):  
Sodium Nitroprusside ◽  
Chlorophyll Content ◽  
Soluble Carbohydrates ◽  
Antioxidant Enzyme Activities ◽  
Content Increase ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
No Donor ◽  
Peanut Cultivars

ABSTRACT As a bioactive signaling molecule, nitric oxide (NO) is involved in multiple plant physiological responses. It regulates diverse biochemical processes in a concentration-dependent manner in plants. Different NO generators viz. sodium nitroprusside (SNP), S-nitroso-N-acetyl penicillinamine (SNAP) and S-nitroso-L-glutathione (GSNO) have been reported, but SNP is the most widely used and effective NO donor. Research was conducted to investigate the in vitro effects of an NO donor, SNP, on biochemical and physiological characteristics such as multiple shoots, chlorophyll content, and enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and others in Arachis hypogaea genotypes (M-13 and PBS24030). In vitro impact of SNP on shoot multiplication potential and chlorophyll content increase upto 100 µM SNP alone in peanut cultivars (M-13 and PBS24030). Rhizogenesis was noticed in the presence of SNP alone. Treatment with SNP and 6-Benzyl adenine (BA) was effective in enhancing the antioxidant enzyme activities, total soluble carbohydrates and proteins as compared to SNP alone in for both cultivars. These data indicate that in vitro establishment of peanut cultivars in the presence of SNP alone and in combination with BA will affect various growth promontory physiological and biochemical parameters. A more complete understanding of plant growth regulator (PGR) mediated responses will be instrumental in designing effective strategies for engineering crops for biotic and abiotic stresses.

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