Effect of regulation of zinc finger protein 281 expression by miR-203 on the proliferation and migration of melanoma cell lines

2020 ◽  
Keyword(s):  
Cell Lines ◽  
Zinc Finger ◽  
Melanoma Cell ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
And Migration ◽  
Melanoma Cell Lines ◽  
Proliferation And Migration

scholarly journals 6-Bromoindirubin-3′oxime (BIO) decreases proliferation and migration of canine melanoma cell lines

2015 ◽  
Vol 205 (2) ◽  
pp. 305-312 ◽  
Author(s):  
Esther Chon ◽  
Brandi Flanagan ◽  
Lucas Campos de Sá Rodrigues ◽  
Caroline Piskun ◽  
Timothy J. Stein
Keyword(s):  
Cell Lines ◽  
Melanoma Cell ◽  
And Migration ◽  
Canine Melanoma ◽  
Melanoma Cell Lines ◽  
Proliferation And Migration

Elucidating distinct tumorigenic pathways in nodular versus superficial spreading melanoma.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 8544-8544
Author(s):  
Joshua Andrew Farhadian ◽  
Kathryn E. O'Reilly ◽  
Eleazar Vega-Saenz de Miera ◽  
Fei Ye ◽  
Jiri Zavadil ◽  
...  
Keyword(s):  
Western Blot ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Growth Phase ◽  
Superficial Spreading ◽  
Superficial Spreading Melanoma ◽  
Metastatic Melanoma Cell ◽  
And Migration ◽  
Melanoma Cell Lines ◽  
Proliferation And Migration

8544 Background: Superficial spreading melanoma (SSM) and nodular melanoma (NM), which account for 70% and 20% of all melanoma diagnoses respectively, are believed to represent sequential phases of linear progression from radial to vertical growth. Recent evidence from several groups including ours challenges this linear progression model and suggests that SSM and NM are biologically distinct. In this study, we focused on identifying tumorigenic pathways that are differentially activated in melanoma subtypes and that can be therapeutically exploited. Methods: We performed Protein Pathway Array on SSM/radial growth phase (RGP) and NM/vertical growth phase (VGP) primary melanoma cell lines to determine expression levels of 141 tumorigenic proteins/phospho-proteins. Differential protein expression was determined using the Pavlidis Template Matching algorithm in TIGR Multi Experiment Viewer. Differentially expressed proteins were validated in an expanded panel of RGP, VGP, and metastatic melanoma cell lines using western blot. Constitutively active, overexpressed proteins in VGP melanoma were down-regulated in cell lines using several small molecule inhibitors and the effects on proliferation and migration were assessed. Results: 17 (12%) proteins/phospho-proteins are significantly overexpressed in VGP versus RGP melanoma (p<0.05). Western blot confirmed the results of 5 proteins/phospho-proteins. Each of those 5 was equally overexpressed in VGP and metastatic melanoma cell lines. Phospho-p90 ribosomal s6 kinase (RSK) was prioritized for functional studies based on its role in both the MAPK and AKT signaling cascades, two tumorigenic pathways often dysregulated in melanoma. Constitutive phosphorylation of p90 RSK in VGP, but not RGP melanoma was observed in response to serum starvation. Small molecule inhibition of phospho-p90 RSK attenuated proliferation and migration (p<0.05) in VGP melanoma. Conclusions: Data demonstrate discrete tumorigenic pathways are activated in nodular versus superficial spreading melanoma and suggest that phospho-p90 RSK might be a viable target against nodular melanoma.

scholarly journals Heterogeneity of Melanoma Cell Responses to Sleep Apnea-Derived Plasma Exosomes and to Intermittent Hypoxia

Cancers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 4781
Author(s):  
Abdelnaby Khalyfa ◽  
Wojciech Trzepizur ◽  
Alex Gileles-Hillel ◽  
Zhuanhong Qiao ◽  
David Sanz-Rubio ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Intermittent Hypoxia ◽  
Melanoma Cells ◽  
Cell Proliferation And Migration ◽  
Exosomal Mirnas ◽  
And Migration ◽  
Melanoma Cell Lines ◽  
Proliferation And Migration

Obstructive sleep apnea (OSA) is associated with increased cutaneous melanoma incidence and adverse outcomes. Exosomes are secreted by most cells, and play a role in OSA-associated tumor progression and metastasis. We aimed to study the effects of plasma exosomes from OSA patients before and after adherent treatment with continuous positive airway pressure (CPAP) on melanoma cells lines, and also to identify exosomal miRNAs from melanoma cells exposed to intermittent hypoxia (IH) or normoxia. Plasma-derived exosomes were isolated from moderate-to-severe OSA patients before (V1) and after (V2) adherent CPAP treatment for one year. Exosomes were co-incubated with three3 different melanoma cell lines (CRL 1424; CRL 1619; CRL 1675) that are characterized by genotypes involving different mutations in BRAF, STK11, CDKN2A, and PTEN genes to assess the effect of exosomes on cell proliferation and migration, as well as on pAMK activity in the presence or absence of a chemical activator. Subsequently, CRL-1424 and CRL-1675 cells were exposed to intermittent hypoxia (IH) and normoxia, and exosomal miRNAs were identified followed by GO and KEG pathways and gene networks. The exosomes from these IH-exposed melanoma cells were also administered to THP1 macrophages to examine changes in M1 and M2 polarity markers. Plasma exosomes from V1 increased CRL-1424 melanoma cell proliferation and migration compared to V2, but not the other two cell lines. Exposure to CRL-1424 exosomes reduced pAMPK/tAMPK in V1 compared to V2, and treatment with AMPK activator reversed the effects. Unique exosomal miRNAs profiles were identified for CRL-1424 and CRL-1675 in IH compared to normoxia, with six miRNAs being regulated and several KEGG pathways were identified. Two M1 markers (CXCL10 and IL6) were significantly increased in monocytes when treated with exosomes from IH-exposed CRL-1424 and CRL-1625 cells. Our findings suggest that exosomes from untreated OSA patients increase CRL-1424 melanoma malignant properties, an effect that is not observed in two other melanoma cell lines. Exosomal cargo from CRL-1424 cells showed a unique miRNA signature compared to CRL-1675 cells after IH exposures, suggesting that melanoma cells are differentially susceptible to IH, even if they retain similar effects on immune cell polarity. It is postulated that mutations in STK-11 gene encoding for the serine/threonine kinase family that acts as a tumor suppressor may underlie susceptibility to IH-induced metabolic dysfunction, as illustrated by CRL-1424 cells.

Proteomic Analysis of MCF-7 Cell Lines Expressing the Zinc-Finger or the Proline-Rich Domain of Retinoblastoma-Interacting-Zinc-Finger Protein

2006 ◽  
Vol 5 (5) ◽  
pp. 1176-1185 ◽  
Author(s):  
Angela Chambery ◽  
Annarita Farina ◽  
Antimo Di Maro ◽  
Mariangela Rossi ◽  
Ciro Abbondanza ◽  
...  
Keyword(s):  
Cell Lines ◽  
Zinc Finger ◽  
Proteomic Analysis ◽  
Zinc Finger Protein ◽  
Rich Domain ◽  
Finger Protein ◽  
Mcf 7

scholarly journals Isogenic Human Cell Lines for Drug Discovery: Regulation of Target Gene Expression by Engineered Zinc-Finger Protein Transcription Factors

2005 ◽  
Vol 10 (4) ◽  
pp. 304-313 ◽  
Author(s):  
Pei-Qi Liu ◽  
Siyuan Tan ◽  
Matthew C. Mendel ◽  
Richard J. Murrills ◽  
Bheem M. Bhat ◽  
...  
Keyword(s):  
Cell Lines ◽  
Zinc Finger ◽  
Human Cell ◽  
Target Gene ◽  
Zinc Finger Protein ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Hek293 Cells ◽  
Human Cell Lines ◽  
Finger Protein

Isogenic cell lines differing only in the expression of the protein of interest provide the ideal platform for cell-based screening. However, related natural lines differentially expressing the therapeutic target of choice are rare. Here the authors report a strategy for drug screening employing isogenic human cell lines in which the expression of the target protein is regulated by a gene-specific engineered zinc-finger protein (ZFP) transcription factor (TF). To demonstrate this approach, a ZFP TF activator of the human parathyroid hormone receptor 1 (PTHR1) gene was identified and introduced into HEK293 cells (negative for PTHR1). Following induction of ZFP TF expression, this cell line produced functional PTHR1 protein, resulting in a robust and ligand-specific cyclic adenosine monophosphate (cAMP) response. Reciprocally, the natural expression of PTHR1 observed in SAOS2 cells was dramatically reduced by the introduction of the appropriate PTHR1-specific ZFP TF repressor. Moreover, this ZFP-driven PTHR1 repression selectively eliminated the functional cAMP response invoked by known ligands of PTHR1. These data establish ZFP TF–generated isogenic lines as a general approach for the identification of therapeutic agents specific for the target gene of interest.

scholarly journals Progression of interleukin-2 (IL-2)-dependent rat T cell lymphoma lines to IL-2-independent growth following activation of a gene (Gfi-1) encoding a novel zinc finger protein.

1993 ◽  
Vol 13 (3) ◽  
pp. 1759-1768 ◽  
Author(s):  
C B Gilks ◽  
S E Bear ◽  
H L Grimes ◽  
P N Tsichlis
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Cell Lymphoma ◽  
Interleukin 2 ◽  
T Cell Lymphoma ◽  
Finger Protein ◽  
Cell Clones

During progression of Moloney murine leukemia virus (Mo-MuLV)-induced rat T cell lymphomas, growth selection results in the expansion of cell clones carrying increasing numbers of integrated proviruses. These new provirus insertions reproducibly contribute to enhanced growth, allowing the emergence of cell clones from the initially heterogeneous population of tumor cells. The Mo-MuLV-induced rat T cell lymphoma lines 2780d and 5675d, which are dependent on interleukin-2 (IL-2) for growth in culture (IL-2d), were placed in IL-2-free medium to select for IL-2-independent (IL-2i) mutants. Southern blot analysis of genomic DNA from these mutants, which was hybridized to a Mo-MuLV long terminal repeat probe, revealed that all mutants carried new provirus insertions (from one to four new proviruses per cell line). A locus of integration identified through cloning of the single new provirus detected in one of the IL-2i mutants, 2780i.5, was found to be the target of provirus insertion in 1 additional IL-2i cell line of 24 tested. A full-length cDNA of a gene (growth factor independence-1 [Gfi-1]) activated by promoter insertion in the 2780i.5 cells was cloned and shown to encode a novel zinc finger protein. Gfi-1 is expressed at low levels in IL-2d cell lines cultured in IL-2-containing medium and at high levels in most IL-2i cell lines, including the two harboring a provirus at this locus. Gfi-1 expression in adult animals is restricted to the thymus, spleen, and testis. In mitogen-stimulated splenocytes, Gfi-1 expression begins to rise at 12 h after stimulation and reaches very high levels after 50 h, suggesting that it may be functionally involved in events occurring after the interaction of IL-2 with its receptor, perhaps during the transition from the G1 to the S phase of the cell cycle. In agreement with this, Gfi-1 does not induce the expression of IL-2. Expression of Gfi-1 in 2780d cells following transfer of a Gfi-1/LXSN retrovirus construct contributes to the emergence of the IL-2i phenotype.

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