Difference in expression of lymphatic markers D2-40 and Prox1 between angiokeratoma corporis diffusum and angioma serpiginosum

2019 ◽  
Vol 52 (11) ◽  
Keyword(s):  
Lymphatic Markers

Lymphatic markers in the human optic nerve

2018 ◽  
Vol 173 ◽  
pp. 113-120 ◽  
Author(s):  
A. Trost ◽  
C. Runge ◽  
D. Bruckner ◽  
A. Kaser-Eichberger ◽  
B. Bogner ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Human Optic Nerve ◽  
Lymphatic Markers

Lymphatic Markers in the Adult Human Choroid

2015 ◽  
Vol 56 (12) ◽  
pp. 7406 ◽  
Author(s):  
Falk Schrödl ◽  
Alexandra Kaser-Eichberger ◽  
Andrea Trost ◽  
Clemens Strohmaier ◽  
Barbara Bogner ◽  
...  
Keyword(s):  
Adult Human ◽  
Lymphatic Markers

Clinical relevance of angiogenesis and lymphangiogenesis in patients with sex cord-stromal ovarian tumors

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 5575-5575
Author(s):  
J. Brown ◽  
M. T. Deavers ◽  
A. M. Nick ◽  
L. Milojevic ◽  
D. M. Gershenson ◽  
...  
Keyword(s):  
Lymph Node ◽  
Distant Metastasis ◽  
Clinical Relevance ◽  
Lymph Node Involvement ◽  
Nodal Metastasis ◽  
Ovarian Tumors ◽  
Vegf Expression ◽  
Institutional Review ◽  
Increased Risk ◽  
Lymphatic Markers

5575 Background: Distant metastasis is common in patients with sex cord-stromal ovarian tumors (SCSTs), but lymph node involvement is extremely rare. Given the lack of lymphatics in the normal granulosa layer, we examined the clinical relevance of angiogenesis and lymphangiogenesis in patients with SCSTs. Methods: After Institutional Review Board approval, 80 tumor samples (46 primary, 34 recurrent) were obtained from 65 patients and stained for CD31 (microvessel density; MVD), D2–40 (lymphvascular density; LVD), and VEGF. Clinical data were extracted by chart review and correlated with the angiogenesis and lymphatic markers. Results: The median age of the patients was 42.1 years (range, 8.6–78.6 years). At presentation, 53% of patients had stage I disease, 8% had stage II, 16% had stage III, and 23% were unstaged. Although VEGF expression was noted at some level in 99% of samples, VEGF overexpression (upper tertile of overall score) was noted in 35% of samples. On the basis of ROC analysis, vessel density was stratified into high versus low at a mean MVD cutoff of 12.3. High MVD was present in 41% of all tumors. High MVD was significantly associated with shorter disease-specific survival (mean 16.7 versus 32.3 months; p = 0.024), increased risk of recurrence (p < 0.04), and shorter overall survival (median 108.6 versus 388.5 months; p < 0.001). High MVD was strongly related to VEGF overexpression (p = 0.009). While VEGF expression was not related to the pattern of recurrence, high MVD was strongly associated with distant metastasis (abdomen, liver, lung, bone) compared with local recurrence (p < 0.001). Lymph node metastasis was clinically evident in only 3 of the 65 patients. Most tumors had either absent or low LVD. Interestingly, all 3 tumors with nodal metastasis had significantly greater LVD (LVD = 35.8 vessels/hpf) compared to those without nodal metastasis (LVD = 2.7 vessels/hpf; p < 0.001). Conclusions: Increased angiogenesis is a significant predictor of poor clinical outcome in patients with sex-cord stromal tumors. Most of these tumors have poor lymphatic development that might explain the low likelihood of nodal metastasis. These data provide support for further investigation of antiangiogenic biological agents in these patients. No significant financial relationships to disclose.

scholarly journals Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges

2019 ◽  
Vol 139 (2) ◽  
pp. 383-401 ◽  
Author(s):  
Shannon Shibata-Germanos ◽  
James R. Goodman ◽  
Alan Grieg ◽  
Chintan A. Trivedi ◽  
Bridget C. Benson ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dura Mater ◽  
Lymphatic Vessels ◽  
Amyloid Β ◽  
Lymphatic Endothelial Cells ◽  
Cell Type ◽  
Functional Conservation ◽  
Spherical Inclusions ◽  
Lymphatic Markers ◽  
The Brain

Abstract The vertebrate CNS is surrounded by the meninges, a protective barrier comprised of the outer dura mater and the inner leptomeninges, which includes the arachnoid and pial layers. While the dura mater contains lymphatic vessels, no conventional lymphatics have been found within the brain or leptomeninges. However, non-lumenized cells called Brain/Mural Lymphatic Endothelial Cells or Fluorescent Granule Perithelial cells (muLECs/BLECs/FGPs) that share a developmental program and gene expression with peripheral lymphatic vessels have been described in the meninges of zebrafish. Here we identify a structurally and functionally similar cell type in the mammalian leptomeninges that we name Leptomeningeal Lymphatic Endothelial Cells (LLEC). As in zebrafish, LLECs express multiple lymphatic markers, containing very large, spherical inclusions, and develop independently from the meningeal macrophage lineage. Mouse LLECs also internalize macromolecules from the cerebrospinal fluid, including Amyloid-β, the toxic driver of Alzheimer’s disease progression. Finally, we identify morphologically similar cells co-expressing LLEC markers in human post-mortem leptomeninges. Given that LLECs share molecular, morphological, and functional characteristics with both lymphatics and macrophages, we propose they represent a novel, evolutionary conserved cell type with potential roles in homeostasis and immune organization of the meninges.

Transmural pressure in rat initial subpleural lymphatics during spontaneous or mechanical ventilation

2005 ◽  
Vol 289 (1) ◽  
pp. H263-H269 ◽  
Author(s):  
Andrea Moriondo ◽  
Sylvain Mukenge ◽  
Daniela Negrini
Keyword(s):  
Mechanical Ventilation ◽  
Spontaneous Breathing ◽  
Lymphatic Vessels ◽  
Respiratory Muscles ◽  
Lymphatic Drainage ◽  
Transmural Pressure ◽  
Hydraulic Pressure ◽  
Alveolar Pressure ◽  
Venous Catheterization ◽  
Lymphatic Markers

The role played by the mechanical tissue stress in supporting lymph formation and propulsion in thoracic tissues was studied in deeply anesthetized rats ( n = 13) during spontaneous breathing or mechanical ventilation. After arterial and venous catheterization and insertion of an intratracheal cannula, fluorescent dextrans were injected intrapleurally to serve as lymphatic markers. After 2 h, the fluorescent intercostal lymphatics were identified, and the hydraulic pressure in lymphatic vessels (Plymph) and adjacent interstitial space (Pint) was measured using micropuncture. During spontaneous breathing, end-expiratory Plymph and corresponding Pint were −2.5 ± 1.1 (SE) and 3.1 ± 0.7 mmHg ( P < 0.01), which dropped to −21.1 ± 1.3 and −12.2 ± 1.3 mmHg, respectively, at end inspiration. During mechanical ventilation with air at zero end-expiratory alveolar pressure, Plymph and Pint were essentially unchanged at end expiration, but, at variance with spontaneous breathing, they increased at end inspiration to 28.1 ± 7.9 and 28.2 ± 6.3 mmHg, respectively. The hydraulic transmural pressure gradient (ΔPtm = Plymph − Pint) was in favor of lymph formation throughout the whole respiratory cycle (ΔPtm = −6.8 ± 1.2 mmHg) during spontaneous breathing but not during mechanical ventilation (ΔPtm = −1.1 ± 1.8 mmHg). Therefore, data suggest that local tissue stress associated with the active contraction of respiratory muscles is required to support an efficient lymphatic drainage from the thoracic tissues.

Characterization of the dental lymphatic system and identification of cells immunopositive to specific lymphatic markers

2009 ◽  
Vol 117 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Ellen Berggreen ◽  
Sivakami R. Haug ◽  
Lilian E. Mkonyi ◽  
Athanasia Bletsa
Keyword(s):  
Lymphatic System ◽  
Lymphatic Markers

scholarly journals Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth

Cancers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 228 ◽  
Author(s):  
Jackelien van Beek ◽  
Quincy van den Bosch ◽  
Nicole Naus ◽  
Dion Paridaens ◽  
Annelies de Klein ◽  
...  
Keyword(s):  
Lymphatic Vessels ◽  
Homeobox Gene ◽  
High Specificity ◽  
Immunohistochemical Markers ◽  
Two Samples ◽  
Marker Cluster ◽  
Lymphatic Markers ◽  
Cluster Of Differentiation ◽  
Paraffin Tissue ◽  
Uveal Melanomas

The aim of this study was to investigate the presence of intraocular lymphatic vessels in patients with uveal melanomas and extrascleral extension using a panel of lymphatic markers. The following immunohistochemical markers were analyzed: lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1), podoplanin (D2-40), prospero-related homeobox gene-1 (Prox-1), pan-endothelial marker cluster of differentiation 31 (CD31), and blood vessel endothelium-specific CD34. Lymphatic vessels were defined as a combination of staining of the following positive markers: LYVE-1, D2-40, Prox-1, and CD31; and no staining of the negative marker CD34. In total, 456 patients were enucleated; 16 of the 46 uveal melanomas with extrascleral extension were contained in stored paraffin tissue. Two samples of the 16 uveal melanomas showed focal positive intraocular vascular staining for LYVE-1 and co-expression of CD31 and CD34. Due to the lack of Prox-1 and D2-40, and positive expression of CD34, these cannot be classified as lymphatic vessels. In one case recruitment of an extraocular, intratumoral lymphatic vascular structure was observed in the periphery of the subconjunctival extrascleral extension. Intraocular lymphatic vessels are absent in uveal melanomas with extrascleral extension; however, we provide proof for recruitment of intratumoral lymphatics by uveal melanomas with extraocular extension from subconjunctival lymphatics that may explain the rare cases of regional lymphatic spread. A panel of antibodies is necessary to detect lymphatic vessels with high specificity.

The association of maternal lymphatic markers and critical congenital heart defects in the fetus-A population based case-control study

2017 ◽  
Vol 173 (5) ◽  
pp. 1231-1236
Author(s):  
Martina A. Steurer ◽  
Mary E. Norton ◽  
Rebecca J. Baer ◽  
Gary M. Shaw ◽  
Sheila Keating ◽  
...  
Keyword(s):  
Congenital Heart Defects ◽  
Congenital Heart ◽  
Case Control Study ◽  
Heart Defects ◽  
Population Based ◽  
Case Control ◽  
Lymphatic Markers ◽  
Control Study

scholarly journals Alzheimer disease and Apolipoprotein E4: meningeal brain lymphatics point to new clues in pathogenesis

2019 ◽  
Author(s):  
Alexios-Fotios A. Mentis ◽  
George P. Chrousos
Keyword(s):  
Amyloid Beta ◽  
Lymphatic System ◽  
Late Onset ◽  
Lymphatic Vessels ◽  
Amyloid Beta Peptide ◽  
Missense Mutations ◽  
New Findings ◽  
Beta Peptide ◽  
Lymphatic Markers ◽  
The Brain

ABSTRACTThe role of the lymphatic system in brain function and/or dysfunction has long been an enigma. However, recent reports that meningeal lymphatic vessels exist within the mouse and human brain, as well as evidence that mouse meningeal lymphatic vessels play a role in clearing the toxic amyloid-beta peptide connected with Alzheimer’s disease (AD), may herald novel diagnostic and therapeutic avenues. Here, we explore new evidence connecting the lymphatic system of the brain with AD. In particular, we focus on new findings showing that meningeal lymphatic vessels play a role in drainage of cerebrospinal fluid and egress of immune cells from the brain, and that disrupting this vessel system leads to accumulation of amyloid - beta peptide and cognitive dysfunction. We also discuss the hypothesis that apolipoprotein E isoform e4 (APO E4) ─ the leading genetic risk for developing AD ─ is involved in meningeal lymphatic vessel function. By reanalyzing previously published RNA-Seq data, we show that APO E4 knock-in microglia cells express lower levels of genes representing lymphatic markers (a phenomenon we call “attenuated lymphaticness”) and of genes in which functional missense mutations are linked to lymphedema. Accordingly, we propose the hypothesis that APO E4 is involved in the shrinkage of lymphatic vessels. This notion could lead, if verified by additional anatomic and mechanistic data, to the concept that APO E4-related AD (such as in late onset AD or trisomy 21-related AD) is related to lymphosclerosis coupled with lymphedema.

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