scholarly journals Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

2019 ◽  
Vol 23 (6) ◽  
pp. 1048-1059 ◽  
Author(s):  
Zeynep FİLİZ ◽  
Tülay OYMAK ◽  
Emrah DURAL
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Array Detector ◽  
Diode Array ◽  
Cosmetic Products ◽  
Diode Array Detector ◽  
Synthetic Colorants

scholarly journals Simultaneous Determination of Isothiazolinones and Parabens in Cosmetic Products Using Solid-Phase Extraction and Ultra-High Performance Liquid Chromatography/Diode Array Detector

2020 ◽  
Vol 13 (11) ◽  
pp. 412
Author(s):  
Hazim Mohammed Ali ◽  
Ibrahim Hotan Alsohaimi ◽  
Mohammad Rizwan Khan ◽  
Mohammad Azam
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Solid Phase ◽  
Array Detector ◽  
Diode Array ◽  
Phase Extraction ◽  
Cosmetic Products ◽  
Diode Array Detector

Isothiazolinones methylisothiazolinone (MI) and methylchloroisothiazolinone (MCI), and parabens methylparaben (MP), ethylparaben (EP), propylparaben (PP) and butylparaben (BP) are the most common synthetic preservatives. They are all known to be potential skin allergens that lead to contact dermatitis. Thus, the identification of these unsafe chemicals in cosmetic products is of high importance. In the present study, solid-phase extraction (SPE) based on HyperSep reversed-phase C8/benzene sulfonic acid ion exchanger (HyperSep C8/BSAIE) and Sep-Pak C18 sorbents, and ultra-high performance liquid chromatography/diode array detector (UHPLC/DAD) were optimized for the simultaneous determination of MI, MCI, MP, EP, PP and BP in cosmetic products. HyperSep C8/BSAIE and UHPLC/DAD with the eluting solvent mixture (acetonitrile/methanol, 2:1, v/v) and detection wavelength (255 nm) were found to be the optimal conditions, respectively. The method illustrates the excellent linearity range (0.008–20 μg/mL) with coefficient of determination (R2, 0.997–0.999), limits of detection (LOD, 0.001–0.002 μg/mL), precision in terms of relative standard deviation (RSD < 3%, intra-day and <6%, inter-day) when examining a standard mixture at low (0.07 µg/mL), medium (3 µg/mL) and high (15 µg/mL) concentrations. A total of 31 cosmetic samples were studied, achieving concentrations (MI, not detected (nd)-0.89 µg/g), (MCI, nd-0.62 µg/g), (MP, nd-6.53 µg/g), (EP, nd-0.90 µg/g), (PP, nd-9.69 µg/g) and (BP, nd-17.80 µg/g). Recovery values ranged from 92.33 to 101.43% depending on the types of sample. To our knowledge, this is the first specific method which covers the theme and describes background amounts of such preservatives in cosmetics.

scholarly journals Determination of phenmedipham and desmedipham in a commercial herbicide by high performance liquid chromatography

2004 ◽  
pp. 193-198 ◽  
Author(s):  
Eva Loncar ◽  
Petar Sekulic ◽  
Tijana Zeremski-Skoric ◽  
Radomir Malbasa ◽  
Ljiljana Kolarov
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phase Separation ◽  
High Performance ◽  
Reversed Phase ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
One Year

Betanal AM-11 is the herbicide that is using to control one-year old weeds with wide leafs in sugar beet fields. Active ingredients of the herbicide are phenmedipham and desmedipham. Commercial emulsifiable concentrate (EC) contains 80 g/L ( 10% of each active compound. We applied high performance liquid chromatography (HPLC) with diode array detector (DAD) at 254 nm for the determination of phenmedipham and desmedipham in commercial samples of Betanal AM-11. This method involves reversed-phase separation of the components on C-18 bonded silica with methanol-water (51+49, v/v) as the eluent. The procedure was highly selective and reproducible and can be successfully used in determining contents of phenmedipham and desmedipham on micro and macro levels.

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