scholarly journals Standardization of quantitative real time PCR for differential diagnostic of Parvovirus B19 infection in acute liver failure patients

2019 ◽  
Author(s):  
Arthur Alves ◽  
Barbara Langella ◽  
Rita Garcia ◽  
Oswaldo Cruz ◽  
Marcelo Pinto ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Real Time ◽  
Real Time Pcr ◽  
Parvovirus B19 ◽  
Quantitative Real Time Pcr ◽  
Parvovirus B19 Infection

Quantitative real-time PCR for differential diagnostics of parvovirus B19 infection in acute liver failure patients

2019 ◽  
Vol 19 (3) ◽  
pp. 259-266 ◽  
Author(s):  
Arthur Daniel Rocha Alves ◽  
Rita De Cassia Nasser Cubel Garcia ◽  
Oswaldo Gonçalves Cruz ◽  
Marcelo Alves Pinto ◽  
Luciane Almeida Amado Leon
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Real Time ◽  
Real Time Pcr ◽  
Parvovirus B19 ◽  
Differential Diagnostics ◽  
Quantitative Real Time Pcr ◽  
Parvovirus B19 Infection

Parvovirus B19 Infection in a Fatal Case of Acute Liver Failure

2017 ◽  
Vol 36 (12) ◽  
pp. e355-e358 ◽  
Author(s):  
Luciane Almeida Amado Leon ◽  
Arthur Daniel Rocha Alves ◽  
Rita de Cássia Nasser Cubel Garcia ◽  
Juliana Gil Melgaço ◽  
Vanessa Salete de Paula ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Parvovirus B19 ◽  
Fatal Case ◽  
Parvovirus B19 Infection

scholarly journals Parvovirus B19 in an Immunocompetent Adult Patient with Acute Liver Failure: An Underdiagnosed Cause of Acute Non-A-E Viral Hepatitis

2005 ◽  
Vol 19 (3) ◽  
pp. 161-162 ◽  
Author(s):  
J Kee Ho ◽  
Susan PL Tha ◽  
Robert Coupland ◽  
Bakul I Dalal ◽  
William R Bowie ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Acute Hepatitis ◽  
Viral Hepatitis ◽  
Adult Patient ◽  
Causative Agent ◽  
Hepatic Failure ◽  
Parvovirus B19 ◽  
Immunocompetent Adult ◽  
Parvovirus B19 Infection

There are occasional pediatric reports of parvovirus B19-associated transient acute hepatitis and hepatic failure. A case of a 34-year-old immunocompetent woman who developed severe and prolonged but self-limited acute hepatitis and myelosuppression following acute parvovirus B19 infection is reported. Parvovirus B19 may be the causative agent in some adult cases of acute non-A-E viral hepatitis and acute liver failure.

scholarly journals Multiplex Qpcr Facilitates Identification Of Betaherpesviruses In Patients With Acute Liver Failure Of Unknown Etiology

2019 ◽  
Author(s):  
Jéssica Vasques Raposo ◽  
Arthur Daniel Rocha Alves ◽  
Alexandre Dos Santos Da Silva ◽  
Damião Carlos Dos Santos ◽  
Juliana Gil Melgaço ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Real Time ◽  
Liver Enzyme ◽  
Real Time Pcr ◽  
Dual Infection ◽  
Unknown Etiology ◽  
Hepatic Enzyme ◽  
Serum Samples ◽  
Multiplex Qpcr

Abstract Background: The etiology of acute liver failure (ALF) is often unknown and reported to be associated with herpesviruses in a number of cases. In this study, we examined for betaherpesviruses infections in patients with ALF of unknown etiology using a multiplex qPCR to Betaherpesviruses subfamily. Methods: Liver and serum samples from 27 patients with ALF of unknown etiology were analyzed with the aid of multiplex qPCR to identify betaherpesviruses. All positive samples were sequenced to confirm herpes infection and liver enzyme levels evaluated. Results: Betaherpesviruses infection was effectively detected using multiplex qPCR. Seven (26%), two (7%) and three (11%) cases were positive for HHV-6, HHV-7 and HCMV, respectively. Two cases of dual infection (HHV-7/HHV-6 and HHV-7/HCMV) were additionally identified. Interestingly, HHV-7 was only detected in the presence of other betaherpesviruses. Sequencing information confirmed betaherpesviruses infection. High hepatic enzyme levels and INR values>1.4 were determined in all betaherpesvirus-positive patients. Conclusions: Multiplex qPCR facilitated efficient quantification, indicating that differentiation between betaherpesviruses is possible with the sole use of real-time PCR. Liver and serum samples were positive for some betaherpesviruses, suggesting an association with ALF. Coinfection of HHV-7 with HHV-6 or HCMV was additionally detected, suggesting that the precursor betaherpesviruses infection can trigger HHV-7 infection. Based on these results, we propose that ALF patients should be screened for the presence of betaherpesviruses.

Acute Liver Failure as the Initial Manifestation of Wilson Disease Triggered by Human Parvovirus B19 Infection

2012 ◽  
Vol 31 (1) ◽  
pp. 103-104 ◽  
Author(s):  
Akira Shiraishi ◽  
Takayuki Hoshina ◽  
Kenji Ihara ◽  
Takehiko Doi ◽  
Shouichi Ohga ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Wilson Disease ◽  
Parvovirus B19 ◽  
Human Parvovirus B19 ◽  
Initial Manifestation ◽  
Parvovirus B19 Infection

scholarly journals Multiplex qPCR Facilitates Identification of Betaherpesviruses in Patients With Acute Liver Failure of Unknown Etiology

2019 ◽  
Author(s):  
Jéssica Vasques Raposo ◽  
Arthur Daniel Rocha Alves ◽  
Alexandre Dos Santos Da Silva ◽  
Damião Carlos Dos Santos ◽  
Juliana Gil Melgaço ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Real Time ◽  
Liver Enzyme ◽  
Real Time Pcr ◽  
Dual Infection ◽  
Unknown Etiology ◽  
Hepatic Enzyme ◽  
Serum Samples ◽  
Multiplex Qpcr

Abstract Background: The etiology of acute liver failure (ALF) is often unknown and reported to be associated with herpesviruses in a number of cases. In this study, we examined for betaherpesviruses infections in patients with ALF of unknown etiology using a multiplex qPCR to Betaherpesviruses subfamily. Methods: Liver and serum samples from 27 patients with ALF of unknown etiology were analyzed with the aid of multiplex qPCR to identify betaherpesviruses. All positive samples were sequenced to confirm herpes infection and liver enzyme levels evaluated. Results: Betaherpesviruses infection was effectively detected using multiplex qPCR. Seven (26%), two (7%) and three (11%) cases were positive for HHV-6, HHV-7 and HCMV, respectively. Two cases of dual infection (HHV-7/HHV-6 and HHV-7/HCMV) were additionally identified. Interestingly, HHV-7 was only detected in the presence of other betaherpesviruses. Sequencing information confirmed betaherpesviruses infection. High hepatic enzyme levels and INR values>1.4 were determined in all betaherpesvirus-positive patients. Conclusions: Multiplex qPCR facilitated efficient quantification, indicating that differentiation between betaherpesviruses is possible with the sole use of real-time PCR. Liver and serum samples were positive for some betaherpesviruses, suggesting an association with ALF. Coinfection of HHV-7 with HHV-6 or HCMV was additionally detected, suggesting that the precursor betaherpesviruses infection can trigger HHV-7 infection. Based on these results, we propose that ALF patients should be screened for the presence of betaherpesviruses.

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