scholarly journals Bacillus subtilis ve Lactobacillus plantarum probiyotik bakterilerinin bazı balık patojenleri üzerine in vitro antagonistik etkisi

2019 ◽  
Vol 4 (2) ◽  
pp. 278-284
Author(s):  
Ekrem Şanver Çelik ◽  
Sebahattin Ergün ◽  
Sevdan Yılmaz
Keyword(s):  
Bacillus Subtilis ◽  
Lactobacillus Plantarum

scholarly journals New crosstalk between probiotics Lactobacillus plantarum and Bacillus subtilis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tao Yu ◽  
Jian Kong ◽  
Li Zhang ◽  
Xinyi Gu ◽  
Mingyu Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Bacillus Subtilis ◽  
Lactobacillus Plantarum ◽  
Bile Salts ◽  
Intestinal Tract ◽  
Molecular Mechanisms ◽  
Oxygen Species ◽  
Intestinal Fluid ◽  
Simulated Intestinal Fluid

Abstract It was reported that oral administration of Bacillus favored the growth of Lactobacillus in the intestinal tract. Here, this phenomenon was confirmed by co-cultivation of Bacillus subtilis 168 and Lactobacillus plantarum SDMCC050204-pL157 in vitro. To explain the possible molecular mechanisms, B. subtilis 168 cells were incubated in simulated intestinal fluid at 37 °C for 24 h, and up to 90% of cells autolysed in the presence of bile salts. Addition of the autolysate to medium inoculated with Lb. plantarum SDMCC050204 decreased the concentration of H2O2 in the culture, alleviated DNA damage and increased the survival of Lb. plantarum, as like the results of exogenous heme addition. These results suggested that the autolysate provided heme, which activated the heme-dependent catalase KatA in Lb. plantarum SDMCC050204. HPLC confirmed the presence of heme in the autolysate. Disruption of the Lb. plantarum SDMCC050204 katA gene abolished the protective effect of the B. subtilis 168 autolysate against H2O2 stress. We thus hypothesized that the beneficial effect of Bacillus toward Lactobacillus was established through activation of the heme-dependent catalase and remission of the damage of reactive oxygen species against Lactobacillus. This study raised new crosstalk between the two frequently-used probiotics, highlighting heme-dependent catalase as the key mediator.

Antibacterial Activity of Dental Restorative Materials

1971 ◽  
Vol 50 (2) ◽  
pp. 382-387 ◽  
Author(s):  
David M. Updegraff ◽  
Robert W.H. Chang ◽  
Richard W. Joos
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Lactobacillus Plantarum ◽  
Bacteriostatic Activity ◽  
Dental Restorative Materials ◽  
Restorative Materials ◽  
Mixed Bacteria ◽  
Dental Restorative

Twenty-one commercial dental restorative materials, aged under various conditions, were checked on two different culture mediums for their antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, a cariogenic streptococcus, and mixed bacteria from the mouth. The duration of bacteriostatic activity was decreased rapidly by leaching in water. Of the three acrylics checked for ability to prevent in vitro carieslike lesions, the acrylic with highest antibacterial activity had the least caries.

scholarly journals Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum

2016 ◽  
Vol 56 (11) ◽  
pp. 1867 ◽  
Author(s):  
Erika C. Lara ◽  
Fernanda C. Basso ◽  
Flávia B. de Assis ◽  
Fernando A. Souza ◽  
Telma T. Berchielli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacillus Subtilis ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
In Vitro Digestibility ◽  
Aerobic Stability ◽  
Organic Matter Digestibility ◽  
Corn Plants

Chemical composition, fermentation characteristics, in vitro digestibility and aerobic stability were evaluated in corn silage inoculated with microbial additives in two different experiments. Inoculant treatments (untreated, Bacillus subtilis and B. subtilis combined with Lactobacillus plantarum) were applied to fresh forages. Chopped corn plants (2B655 Hx) were ensiled in laboratory silos for periods of 7, 14, 21 and 63 days to evaluate the fermentation parameters. The experimental silos were weighed to determine gas losses. After the ensiling period, the silage was sampled to determine chemical composition and in vitro organic matter digestibility. To evaluate aerobic stability, chopped corn plants (AG‐1051) were ensiled in laboratory silos that were opened after 96 days of ensiling. The silage was placed in different buckets containing data loggers. The silage was sampled after 0, 4, 8 and 12 days of exposure to air to evaluate the microbial populations and pH. The data were analysed as a completely randomised design using a mixed repeated-measures model in the MIXED procedure of SAS. To evaluate each treatment relative to the fermentation times, a regression analysis using the PROC REG procedure of SAS was applied. A significance level of P < 0.05 was used. Inoculation with both strains increased lactic acid concentration, whereas the use of B. subtilis alone or combined with L. plantarum improved in vitro apparent organic matter digestibility. In the B. subtilis and B. subtilis combined with L. plantarum silages, moulds and yeasts decreased, and aerobic stability was improved. Inoculation with B. subtilis alone or combined with L. plantarum improved the nutritional value and aerobic stability of corn silage.

scholarly journals Preparation and in vitro Evaluation of High Performance Fermented Soybean Meal using a mixture of Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae

2019 ◽  
Author(s):  
Jiajia Zhu ◽  
Shuaiyu Wang ◽  
Mingxing Gao ◽  
Yanping Zhang ◽  
Qihe Tang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Bacillus Subtilis ◽  
Lactobacillus Plantarum ◽  
Trypsin Inhibitor ◽  
Soybean Meal ◽  
High Performance ◽  
Amino Acid Content ◽  
Systematic Analysis ◽  
Nutritional Factors

Abstract Background: Soybean meal is used in animal feeds as a protein supplement but contains significant amounts of anti-nutritional factors such as trypsin inhibitor that directly inhibit digestion, absorption and utilization of nutrients. Results: Herein, we used solid state batch fermentation of soybean meal using Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae alone and in combination to alter the content of anti-nutritional compounds in the meal. A systematic analysis identified a temperature of 40℃, water content of 40 % in a 48 h fermentation that optimized the fermentation according to our goals. The digestibility of the meal increased within 36 h and by 48 h, the free amino acid content increased while glycinin, β-conglycinin and trypsin inhibitor significantly decreased and the indigestible sugars stachyose and raffinose were reduced to undetectable levels. These processes correlated with high levels of lactic acid that were responsible for an overall pH decrease. Conclusions: Bacterial and mixed fermentations increased nutritional content while eliminating anti-nutritional factors and significantly improved the nutritional quality of the soybean meal.

Improved Production of Two Anti-Candida Lipopeptide Homologues Co- Produced by the Wild-Type Bacillus subtilis RLID 12.1 under Optimized Conditions

2020 ◽  
Vol 21 (5) ◽  
pp. 438-450
Author(s):  
Ramya Ramchandran ◽  
Swetha Ramesh ◽  
Anviksha A ◽  
RamLal Thakur ◽  
Arunaloke Chakrabarti ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pathogenic Fungi ◽  
Fold Increase ◽  
Production Yield ◽  
Yield Enhancement ◽  
Bioactive Metabolites ◽  
Candida Auris ◽  
Media Formulation ◽  
Static Conditions

Background:: Antifungal cyclic lipopeptides, bioactive metabolites produced by many species of the genus Bacillus, are promising alternatives to synthetic fungicides and antibiotics for the biocontrol of human pathogenic fungi. In a previous study, the co- production of five antifungal lipopeptides homologues (designated as AF1, AF2, AF3, AF4 and AF5) by the producer strain Bacillus subtilis RLID 12.1 using unoptimized medium was reported; though the two homologues AF3 and AF5 differed by 14 Da and in fatty acid chain length were found effective in antifungal action, the production/ yield rate of these two lipopeptides determined by High-Performance Liquid Chromatography was less in the unoptimized media. Methods:: In this study, the production/yield enhancement of the two compounds AF3 and AF5 was specifically targeted. Following the statistical optimization (Plackett-Burman and Box-Behnken designs) of media formulation, temperature and growth conditions, the production of AF3 and AF5 was improved by about 25.8- and 7.4-folds, respectively under static conditions. Results:: To boost the production of these two homologous lipopeptides in the optimized media, heat-inactivated Candida albicans cells were used as a supplement resulting in 34- and 14-fold increase of AF3 and AF5, respectively. Four clinical Candida auris isolates had AF3 and AF5 MICs (100 % inhibition) ranging between 4 and 16 μg/ml indicating the lipopeptide’s clinical potential. To determine the in vitro pharmacodynamic potential of AF3 and AF5, time-kill assays were conducted which showed that AF3 (at 4X and 8X concentrations) at 48h exhibited mean log reductions of 2.31 and 3.14 CFU/ml of C. albicans SC 5314, respectively whereas AF5 at 8X concentration showed a mean log reduction of 2.14 CFU/ml. Conclusion:: With the increasing threat of multidrug-resistant yeasts and fungi, these antifungal lipopeptides produced by optimized method promise to aid in the development of novel antifungal that targets disease-causing fungi with improved efficacy.

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