Use of the PCR-DGGE method in forensic microbiology

2016 ◽  
Vol 292 ◽  
pp. 59-64
Author(s):  
Aleksandra Ziembińska-Buczyńska ◽  
◽  
Krzysztof Kraśnicki ◽  
Keyword(s):  
Mobile Phones ◽  
Electronic Device ◽  
Screening Method ◽  
Electrophoresis Method ◽  
Forensic Microbiology ◽  
The Subject ◽  
Polymerase Chain ◽  
The Individual ◽  
High Degree ◽  
Degree Of Similarity

The latest reports on the subject of forensic microbiology indicate that it is possible to link a perpetrator of an offence to the evidence, based on molecular analysis of microbiological material. The microbiome of human epidermis is known to be species- and individual-specific. This knowledge can be used towards finding a match between the object (e.g. electronic device) and the user, based on the individual-specific microbiome deposited onto the object’s surface. The DNA fingerprinting-based methods were used to compare similarities in the structures of bacterial communities collected from the epidermis of 14 study subjects and the housings of their mobile phones. This study was based on the Polymerase Chain Reaction – Denaturing Gel Electrophoresis method. The results obtained revealed a high degree of similarity between the structure of bacterial genotypes present on the users’ epidermis and the microbiomes recovered from their mobile phones. PCR-DGGE can be used as the screening method, preceding the additional confirmatory analyses.

scholarly journals The analysis of individual Visegrad group members’ agrarian export sensitivity in relation to selected macroeconomic aggregations

2011 ◽  
Vol 59 (4) ◽  
pp. 327-342
Author(s):  
Miroslav Svatoš ◽  
Luboš Smutka
Keyword(s):  
Agricultural Trade ◽  
Added Value ◽  
The Czech Republic ◽  
Grocery Industry ◽  
Agricultural Exports ◽  
Group Members ◽  
The Subject ◽  
The Individual ◽  
High Degree ◽  
Visegrad Group

This paper analyzes the development of agricultural trade of the countries of the Visegrad Group with emphasis on development of the value of agricultural exports of the individual countries. The subject matter of the analysis is the sensitivity of the commodity structure of agricultural exports of individual countries and the identification of aggregations that are the least and the most sensitive to changes to the external and internal economic environment. From the conducted research, agricultural trade in the V4 countries was found to have developed very dynamically from 1993 to 2008, while the commodity structure of exports has constantly narrowed as the degree of specialization of the individual countries has increased (this applies especially to the Czech Republic, Slovakia and Hungary). From the results of analysis of sensitivity to changes of selected variables relating to the development of the value of agricultural exports of the individual V4 countries, it appears that the aggregations that react most sensitively to changes are those that are the subject of re-exports, followed by the aggregations that are characterized by a high degree of added value. In general it can be said that products of agricultural primary production exhibit less sensitivity in comparison with grocery industry products. This is confirmed by the general trend arising from the very nature of consumer behaviour.

The relaxed crossbridge pattern in isolated rabbit psoas muscle thick filaments

1993 ◽  
Vol 105 (3) ◽  
pp. 841-848
Author(s):  
R.W. Kensler ◽  
M. Stewart
Keyword(s):  
Structural Difference ◽  
Psoas Muscle ◽  
Thick Filament ◽  
Rabbit Muscle ◽  
Fish Muscles ◽  
Thick Filaments ◽  
Filament Structure ◽  
The Individual ◽  
High Degree ◽  
Degree Of Similarity

Rabbit muscle is a major source of material for biochemical experiments and spin labelling studies of contraction, and so it is important to establish how closely this material resembles the frog and fish muscles usually used for structural studies. Previous studies have shown that relaxed rabbit muscle thick filaments lose the characteristic order of their crossbridges when they are cooled below about 15–19 degrees C, whereas the order of fish and frog muscles is retained above 0 degrees C. The lack of order has frustrated attempts to examine rabbit thick filament structure and has raised questions about how closely they might resemble other thick filaments. We have therefore developed a procedure for preserving the crossbridge order in isolated filaments. Electron microscopy of these thick filaments after either negative staining or metal shadowing has shown that the crossbridge pattern has a 43 nm axial repeat and is based on three near-helical strands. Computed transforms of either type of image show a series of layer lines confirming that the native relaxed pattern has been preserved, and computer reconstructions show the individual crossbridges lying on a slightly perturbed 3-stranded lattice. These data indicate an unexpectedly high degree of similarity between the rabbit and frog patterns and indicate that, in fully preserved material, there is little structural difference between the two thick filaments at the temperature at which each normally functions.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals DNA Marker-based Study of Genetic Relatedness in United States Sweetpotato Cultivars

1996 ◽  
Vol 121 (6) ◽  
pp. 1059-1062 ◽  
Author(s):  
C.S. Prakash ◽  
Guohao He ◽  
Robert L. Jarret
Keyword(s):  
Ipomoea Batatas ◽  
Dna Marker ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Dna Amplification ◽  
Population Based ◽  
Pedigree Information ◽  
Polymerase Chain ◽  
High Degree ◽  
Degree Of Similarity

The polymerase chain reaction (PCR)-based DNA amplification fingerprinting (DAF) approach was used to investigate genetic relationships among 30 U.S. sweetpotato (Ipomoea batatas L. Lam.) genotypes including heirloom cultivars and recent releases. Phenogram, pairwise similarity matrix, and principal coordinate plots were developed based on Jaccard's coefficients using band-sharing data generated by seven octamer primers. All cultivars showed unique fingerprint patterns indicating the utility of DAF in cultivar identification. Many heirloom cultivars such as `Creole' and `Porto Rico' were readily differentiated from recently developed cultivars. Modern cultivars such as `Jewel', `Carver', `Nugget', and `Scarlet' exhibited a high degree of similarity reflecting ancestral relatedness. `Regal' and `Excel', recently developed using a population-based breeding approach, showed greater divergence from all other cultivars. Those cultivars, developed as a result of somatic mutations, exhibited high levels of genetic similarity to their normal-type parents and yet had distinct fingerprint profiles. With few exceptions, genetic relationships derived from DAF data appear to be consistent with available pedigree information.

scholarly journals Associative-verbal model of the verb haben and its synonyms in German

2019 ◽  
Vol 17 (2) ◽  
pp. 53-64
Author(s):  
N. V. Kozlova
Keyword(s):  
World View ◽  
German Language ◽  
Word Associations ◽  
Language And Culture ◽  
Depth Study ◽  
Systematic Character ◽  
The Subject ◽  
The Individual ◽  
High Degree ◽  
Verbal Model

An associative experiment, widely used in recent years in psycholinguistic studies, allows for an in-depth study of the semantics of a word by considering logical relationships in associative fields. In addition, the results of such an experiment provide “access” to the speakers’ language consciousness and enable the researcher to describe its structure. At the same time, the “construction” of associative-verbal models makes it possible to understand and study the systematic character of the individual speaker’s world view and language consciousness of the speakers of a certain language and culture. All this determines the relevance of the associative-verbal models and specific lexemes analysis. The study of the speaking personality – Homo Loquens – is connected with the analysis of an individual world view explicated in language consciousness and actually existing in the form of verbal images – word associations. This article discusses the results of a free associative experiment with participation of the native German speakers (as a result of this experiment, 137 different associative reactions were received with the total number of associative reactions 305; the analysis focused on both types of responses – primary and secondary word associations). The subject matter of the study is the verb haben, forming the centre in the sphere “possession” and its synonyms (gehören, verfügen, besitzen, gebieten). The synonyms were identified through a lexicographical analysis with the help of five dictionaries of synonyms and the Contemporary German language corpora dwds.de. The analysis is based on a systematic approach that allows us to reveal the structure of senses and meanings in the mental lexicon and the interconnectedness of associative fields of synonymous stimulus words. The lexicographical, corpus and associative types of analysis reveal the following: 1) a negligible number of respondents' refusals to respond; 2) associating with a “paradigmatic” type in regard to the semantic structure of the stimulus word and associative response; 3) a high degree of uniqueness of “syntagmatic” associations; 4) “possession” is associated with such reactions as Geld, Gut, Güter, Macht, Testament, Freunde, nichts.

scholarly journals The study of similarities among Czech alfalfa (Medicago sativa L.) varieties

2014 ◽  
Vol 62 (5) ◽  
pp. 971-977 ◽  
Author(s):  
Daniela Knotová ◽  
Jiří Skládanka ◽  
Jan Pelikán
Keyword(s):  
Dry Matter ◽  
Intraspecific Variability ◽  
Terminal Leaflet ◽  
Individual Trait ◽  
Green Matter ◽  
Medicago Sativa L ◽  
The Individual ◽  
High Degree ◽  
Degree Of Similarity ◽  
Better Than

Within the period of 2010–2011, a set of 14 Czech alfalfa cultivars was evaluated in the research locality Troubsko. On small plots and also in the individual plantations altogether 51 descriptors were evaluated. Statistical differences existing among cultivars in individual trait sunder study were analysed using the method of point and interval estimations of average values. Intraspecific variability was studied on the base of estimation of variation coefficients. A high degree of intraspecific uniformity was found in cultivars ‘Jarka’ and ‘Holyna’ while the lowest one was registered in the cultivar ‘Litava’. Green matter yields ranged from 66.3 t.ha−1 (‘Jitka’) to 77.2 t.ha−1 (‘Holyna’), hay yields from 11.71 t.ha−1 (‘Jitka’) to 15.43 t.ha−1 (‘Jarka’), and yields of seed from 0.19 t.ha−1 (‘Jitka’) to 0.35 t.ha−1 (‘Kamila’). As far as the descriptor “Alfalfa tuft dry matter” was concerned, cultivars ‘Oslava’ and ‘Holyna’ were statistically highly significantly (P < 0.01) better than than cultivars ‘Jitka’ and ‘Vlasta’. As far as the descriptor “Width of the terminal leaflet” was concerned, the cultivar ‘Palava’ was statistically highly significantly better (P < 0.01) than cultivars ‘Vlasta’, ‘Litava’, and ‘Denisa’. The existence of similarity of cultivars under study was determined by means of cluster analysis. It was found out that the highest degree of similarity existed between cultivars ‘Morava’ and ‘Niva’.

cDNA cloning of a renal Na(+)-Ca2+ exchanger

1992 ◽  
Vol 262 (6) ◽  
pp. F1105-F1109 ◽  
Author(s):  
R. F. Reilly ◽  
C. A. Shugrue
Keyword(s):  
Amino Acids ◽  
Northern Blot Analysis ◽  
Renal Cortex ◽  
Cytoplasmic Domain ◽  
Rabbit Kidney ◽  
Hydrophobic Membrane ◽  
Relative Molecular Weight ◽  
Polymerase Chain ◽  
High Degree ◽  
Degree Of Similarity

In the present study, the polymerase chain reaction (PCR) and library screening were used to clone a cDNA for a rabbit kidney Na(+)-Ca2+ exchanger on the basis of homology with the canine cardiac sarcolemmal sequence (D. A. Nicoll, S. Longoni, and K. D. Philipson. Science Wash. DC 250:562-565, 1990). There is a high degree of similarity between the two sequences, with nucleotide identities of 95, 89, and 90% in the hydrophobic membrane-associated domain, cytoplasmic domain, and 3'-untranslated region, respectively. The rabbit kidney cDNA encodes a predicted protein of 941 amino acids, 29 amino acids shorter than the canine sequence, with a relative molecular weight of 105,121. The deduced amino acid sequence is 96% identical in the membrane-associated domain and 94% identical in the cytoplasmic domain. Northern blot analysis reveals that the cDNA is expressed in the renal cortex. No expression is detected in the medulla. This result is in agreement with micropuncture studies that show Na(+)-Ca2+ exchanger activity in cortical but not medullary nephron segments.

Differential Effects of Competence and Morality on Self-Esteem at the Individual and the Collective Level

2020 ◽  
Vol 51 (3) ◽  
pp. 183-198
Author(s):  
Wiktor Soral ◽  
Mirosław Kofta
Keyword(s):  
Self Esteem ◽  
Differential Effects ◽  
Individual Level ◽  
The Subject ◽  
The Individual

Abstract. The importance of various trait dimensions explaining positive global self-esteem has been the subject of numerous studies. While some have provided support for the importance of agency, others have highlighted the importance of communion. This discrepancy can be explained, if one takes into account that people define and value their self both in individual and in collective terms. Two studies ( N = 367 and N = 263) examined the extent to which competence (an aspect of agency), morality, and sociability (the aspects of communion) promote high self-esteem at the individual and the collective level. In both studies, competence was the strongest predictor of self-esteem at the individual level, whereas morality was the strongest predictor of self-esteem at the collective level.

Offer of methods for minimizing key investment risks of “R-Pharm” JSC

2020 ◽  
Vol 17 (1) ◽  
pp. 58-67
Author(s):  
N. A. Kabanova ◽  
I. K. Alekseeva
Keyword(s):  
Strategic Planning ◽  
Pharmaceutical Company ◽  
Simulation Modeling ◽  
Return On Investment ◽  
System Analysis ◽  
Social Orientation ◽  
Pharmaceutical Companies ◽  
The Subject ◽  
High Degree

The article is devoted to the assessment of potential investment risks of the pharmaceutical company “R-Pharm” JSC with the aim of identifying the highest priority risks and developing methods for minimizing them. The relevance of the study is determined by the fact that the pharmaceutical business is characterized by a high degree of social orientation and annually invests $ 140 billion in the development of production and research, which determines the need for a risk-based approach to ensure the return on investment. The subject of this article is the investment risks of pharmaceutical companies, and the subject of research is the domestic pharmaceutical company “R-Pharm”. In order to assess the potential investment risks of “R-Pharm” JSC, the authors used elements of simulation modeling and system analysis. The proposed methods to minimize key investment risks are aimed at improving the efficiency of investment activities and is recommended as an element of the strategic planning of the company.

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

The Autobiographical Subject and the Death Penalty

2013 ◽  
Vol 35 (2) ◽  
pp. 165-187
Author(s):  
E. S. Burt
Keyword(s):  
Death Penalty ◽  
First Person ◽  
Philosophical Investigation ◽  
The Subject ◽  
Definition Of ◽  
The Individual

Why does writing of the death penalty demand the first-person treatment that it also excludes? The article investigates the role played by the autobiographical subject in Derrida's The Death Penalty, Volume I, where the confessing ‘I’ doubly supplements the philosophical investigation into what Derrida sees as a trend toward the worldwide abolition of the death penalty: first, to bring out the harmonies or discrepancies between the individual subject's beliefs, anxieties, desires and interests with respect to the death penalty and the state's exercise of its sovereignty in applying it; and second, to provide a new definition of the subject as haunted, as one that has been, but is no longer, subject to the death penalty, in the light of the worldwide abolition currently underway.

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