scholarly journals Indicator Organisms, Detection of Indicator Organisms, Microbiological Culture Methods and Immunological Methods Employed in Food Industry

2020 ◽  
pp. 94-105
Author(s):  
Deepa I ◽  
Keyword(s):  
Food Industry ◽  
Immunological Methods ◽  
Indicator Organisms ◽  
Culture Methods ◽  
Microbiological Culture

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens

2021 ◽  
Vol 70 (9) ◽  
Author(s):  
Berta Fidalgo ◽  
Elisa Rubio ◽  
Victor Pastor ◽  
Marta Parera ◽  
Clara Ballesté-Delpierre ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Enteric Pathogens ◽  
Pcr Analysis ◽  
Culture Methods ◽  
Gastrointestinal Infections ◽  
Content Type ◽  
Link Type ◽  
Microbiological Culture ◽  
Micro Organisms

Introduction. The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis. Hypothesis/Gap Statement. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination. Aim.The diagnostic performance of the FMPS was evaluated and compared to that of traditional microbiological procedures. Methodology. A total of 10 659 samples were collected and analysed over a period of 7 years. From 2013 to 2018 (every July to September), samples were processed using standard microbiological culture methods. In 2019, the FMPS was implemented using real-time PCR to detect the following enteropathogens: Shigella spp., Salmonella spp., Campylobacter spp., Giardia intestinalis, Entamoeba histolytica, Blastocystis hominis, Cryptosporidum spp., Dientamoeba fragilis, adenovirus, norovirus and rotavirus. Standard microbiological culture methods (2013–2018) included stool culture, microscopy and immunochromatography. Results. A total of 1078 stool samples were analysed prospectively using the FMPS from July to September (2019): bacterial, parasitic and viral pathogens were identified in 15.3, 9.71 and 5.29 % of cases, respectively. During the same period of 6 years (2013–2018), the proportion of positive identifications using standard microbiological methods from 2013 to 2018 was significantly lower. A major significant recovery improvement was observed for all bacteria species tested: Shigella spp./enteroinvasive Escherichia coli (EIEC) (P <0.05), Salmonella spp. (P <0.05) and Campylobacter spp. (P <0.05). Marked differences were also observed for the parasites G. intestinalis, Cryptosporidium spp. and D. fragilis. Conclusion. These results support the value of multiplex real-time PCR analysis for the detection of enteric pathogens in laboratory diagnosis with outstanding performance in identifying labile micro-organisms. The identification of unsuspected micro-organisms for less specific clinical presentations may also impact on clinical practice and help optimize patient management.

scholarly journals Organization of Laboratory for Monitoring Security in the Food Industry in Order to Detect the Presence of Allergens

2016 ◽  
Vol 13 (1-2) ◽  
Author(s):  
Vesna Gojković ◽  
Mirjana Beribaka ◽  
Željka Marjanović-Balaban
Keyword(s):  
High Performance ◽  
Food Industry ◽  
Professional Staff ◽  
Immunological Methods ◽  
Specific Response ◽  
Allergic Reactions ◽  
Polymerase Chain ◽  
Main Factor

Allergens are substances that cause allergic reactions. Allergic reactions differ from person to person in a sensitive and specific response to the presence of the same allergen. Groceries that often cause allergies are cow’s milk, eggs, fish, crustaceans and shellfish, wheat, soy, peanuts, walnuts, almonds, hazelnuts and strawberries.Organisation is the main factor for the success and the quality of a research in food industry laboratories, in order to detect the presence of allergens. All kinds of equipments are needed, as well as professional staff to perform the tests. Allergen testing in the food industry is often performed using biochemical and separation methods. For analysis of deoxyribonucleic acid (DNA), the most suitable method is polymerase chain reaction (PCR) and electrophoresis. In our laboratory, we use immunological methods for qualitative and quantitative testing of allergens and we have two accredited methods: Enzyme-Linked Immunosorbent Assays (ELISA) and High Performance Liquid Chromatography (HPLC). It is also necessary that stuff have adequate competence in handling the specific equipment, performing tests, evaluating the results and signing test reports and calibration certificates, have adequate competences. Laboratory have to prove that have been fulfiled all the requirements for validation. Validation includes: specification of requirements, characterization of method, verification that requirements can be fulfilled using the method.The results of each test are presented in form of a report, which has to be correct, clear, unambiguous, objective and must include all the informations required by the client.

Comparison of microbiological culture methods in screening allograft tissue. Swab versus nutrient broth

2007 ◽  
Vol 70 (2) ◽  
pp. 374-378 ◽  
Author(s):  
Veroniek S.M. Saegeman ◽  
Daniel Lismont ◽  
Bert Verduyckt ◽  
Nadine L. Ectors ◽  
Jan Verhaegen
Keyword(s):  
Nutrient Broth ◽  
Culture Methods ◽  
Microbiological Culture ◽  
Allograft Tissue

scholarly journals Matrix Extension Study: Listeria Right Now™ Test for Detection of Listeria spp. from Selected Environmental Surfaces Without Enrichment: AOAC Performance Tested MethodSM 081802

2019 ◽  
Vol 102 (5) ◽  
pp. 1589-1594
Author(s):  
Brooke Roman ◽  
Mark Mozola ◽  
Robert Donofrio ◽  
Benjamin Bastin ◽  
Nicole Klass ◽  
...  
Keyword(s):  
Ceramic Tile ◽  
Food Industry ◽  
Probability Of Detection ◽  
Extension Study ◽  
Culture Methods ◽  
Detection Model ◽  
Detection Analysis ◽  
Environmental Surfaces ◽  
Matrix Extension ◽  
Culture Procedure

Abstract Background: Listeria Right Now™ is a novel, enrichment-free test for the detection of Listeria spp. in swab samples taken from environmental surfaces. Results are available in less than 1 h. In a previous Performance Tested MethodSM (PTM) study, the test was validated for swab samples from stainless-steel and sealed concrete surfaces. Objective: A PTM matrix extension study was conducted to validate the method for the detection of Listeria spp. in swab samples from ceramic tile, plastic, and rubber surfaces. Methods: Performance of the Listeria Right Now method was compared to that of the U.S. Food and Drug Administration Bacteriological Analytical Manual reference culture procedure for the detection of Listeria spp. in swab samples taken from inoculated ceramic tile, plastic, and rubber surfaces. Data were analyzed using a probability of detection model. Results: There were no significant differences in performance between the Listeria Right Now and reference culture methods for any of the three surfaces tested, as determined by probability of detection analysis. Conclusions: The Listeria Right Now method is an effective procedure for the detection of Listeria spp. from a variety of environmental surfaces. Highlights: Listeria Right Now provides accurate results, without enrichment, in real time. This enables food industry personnel to react swiftly to suspected Listeria contamination incidents.

scholarly journals The microbiota of the surface, dermis and subcutaneous tissue of dog skin

2020 ◽  
Author(s):  
Rocío García-Fonticoba ◽  
Lluis Ferrer ◽  
Olga Francino ◽  
Anna Cusco
Keyword(s):  
Subcutaneous Tissue ◽  
Dna Amplification ◽  
Skin Surface ◽  
Molecular Techniques ◽  
The Other ◽  
Rrna Gene ◽  
Culture Methods ◽  
Bacterial Dna ◽  
Microbiological Culture ◽  
Two Samples

Abstract Background. Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of dogs without cutaneous disease using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS).Results. Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks.Conclusion. When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue.

Microbiological Culture Methods for Pediatric Musculoskeletal Infection

2015 ◽  
Vol 97 (6) ◽  
pp. 441-449 ◽  
Author(s):  
Jarren Section ◽  
Steven D. Gibbons ◽  
Theresa Barton ◽  
David E. Greenberg ◽  
Chan-Hee Jo ◽  
...  
Keyword(s):  
Culture Methods ◽  
Musculoskeletal Infection ◽  
Microbiological Culture

scholarly journals The microbiota of the surface, dermis and subcutaneous tissue of dog skin

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Rocío García-Fonticoba ◽  
Lluís Ferrer ◽  
Olga Francino ◽  
Anna Cuscó
Keyword(s):  
Subcutaneous Tissue ◽  
Dna Amplification ◽  
Skin Surface ◽  
Molecular Techniques ◽  
The Other ◽  
Rrna Gene ◽  
Culture Methods ◽  
Bacterial Dna ◽  
Microbiological Culture ◽  
Two Samples

Abstract Background Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of dogs without cutaneous disease using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS). Results Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks. Conclusion When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue.

scholarly journals Chromatographic Techniques for Estimation of Aflatoxins in Food Commodities

2021 ◽  
Author(s):  
Mateen Abbas
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Food Industry ◽  
Analytical Techniques ◽  
Immunological Methods ◽  
Food Contaminants ◽  
Analytical Technique ◽  
Chromatographic Techniques ◽  
Qualitative Detection ◽  
Food Commodities

Aflatoxins, produced mainly by Aspergillus flavus Aspergillus parasiticus, have been documented as one of the major food contaminants throughout the world. Because of their toxic nature, these food contaminants have acknowledged considerable attention in recent years. Among the different types of Aflatoxins, the most prevalent and predominant Aflatoxins are AFB1, AFB2, AFG1, AFG2, AFM1, AFM2 which are considered the more lethal as compared to others. Several analytical and immunological methods are available for testing and estimating aflatoxins in different food commodities. However, chromatographic techniques have been considered superior regarding the estimation of aflatoxins both qualitatively and quantitatively. Chromatographic techniques have numerous applications for the separation and identification of chemical and biological compounds in food industry. It has grown to be the most popular and versatile of all analytical techniques in laboratories used for the analysis of multiple components in different matrices. For preliminary qualitative detection of Aflatoxins, Thin layer chromatography (TLC) is considered the best analytical technique which is being used broadly in food industry. However, liquid chromatographic techniques including High Performance Liquid Chromatography (HPLC) and Liquid chromatography-mass Spectrometry (LC–MS) are the best analytical techniques developed so far for the quantification of Aflatoxins in food commodities.

scholarly journals The microbiota of the surface, dermis and subcutaneous tissue of dog skin

2020 ◽  
Author(s):  
Rocío García-Fonticoba ◽  
Lluis Ferrer ◽  
Olga Francino ◽  
Anna Cusco
Keyword(s):  
Subcutaneous Tissue ◽  
Skin Surface ◽  
Molecular Techniques ◽  
The Other ◽  
Rrna Gene ◽  
Culture Methods ◽  
Bacterial Dna ◽  
Microbiological Culture ◽  
Two Samples ◽  
Healthy Dogs

Abstract Background. Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. This possibility, however, has not been investigated in the dog so far. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of healthy dogs using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS). Results. Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks. Conclusion. When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue.

scholarly journals Immunological Methods in Gluten Risk Analysis: A Snapshot

Safety ◽  
2018 ◽  
Vol 4 (4) ◽  
pp. 56 ◽  
Author(s):  
Francesca Melini ◽  
Valentina Melini
Keyword(s):  
Risk Assessment ◽  
Risk Analysis ◽  
Food Industry ◽  
Food Choices ◽  
Immunological Methods ◽  
Gluten Free ◽  
Food Allergens ◽  
Food Matrix ◽  
Matrix Type ◽  
Safe Food

Gluten is among the 14 major food allergens officially recognized by Regulation (EU) No. 1169/2011. The risk to coeliac patients from gluten presence in the food products they consume is likely due to the unintentional contamination of naturally gluten-free (GF) and GF-labelled products, or to hidden sources of gluten in processed GF products. The aim of this paper is to provide a snapshot of gluten risk analysis, with emphasis on immunological methods currently used in gluten detection. The study highlights that immunoassays have some advantages over other analytical methods in gluten determination and are suitable for routine tests. However, some factors (e.g., complexity of the food matrix, type of the applied antibody, gluten extraction procedures and lack of reference material) affect the reliability of obtained results. Hence, efforts are required at an analytical level to overcome the drawbacks of the immunological methods currently available. Harmonization is necessary, so as to assist both consumers in making safe food choices, and the food industry in gluten risk assessment, management and communication.

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