scholarly journals The relationship between the expression of PLIN3 and PLIN5 protein flowing endurance training in streptozotocin rats

2019 ◽  
Vol 21 (4) ◽  
pp. 194-199
Author(s):  
Mahdi Ghafari ◽  
Ebrahim Banitalebi ◽  
Mohamad Faramarzi

Background and aims: Intermuscular lipolysis disorder plays an important role in insulin resistance and diabetes mellitus and perilipin PLIN5 and PLIN3 are the key proteins in regulating muscle cellular lipolysis. Therefore, the purpose of this study was to examine the relationship between the expression of PLIN3 and PLIN5 protein following endurance training in streptozotocin (STZ) rats. Methods: A number of 24 male Wistar rats were randomly divided into low endurance training group (n = 8), high-intensity training group (n = 8), and control group (n = 8). Diabetes was induced in every rat by STZ injection. Three days after injection, the blood samples were taken from the cut tip of the tails of the mice and animals with blood glucose greater than 300 mg/dL were considered diabetic. The training program included eight weeks of aerobic training at different intensities. Training in high- and low-intensity groups included 22-25 and 5-8 m/min of training. Finally, one-way analysis of variance (ANOVA) and correlation was used to determine the significance of the differences between variables, followed by utilizing Tukey’s post-hoc test for significance. Results: The comparison between the groups by ANOVA showed significant differences in PLIN3 (P=0.0006) and PLIN5 (P=0.012). The results of Tukey post hoc test also demonstrated a statistical difference between the mean values of diabetic control group and high-intensity endurance group regarding PLIN3 (P=0.01) and PLIN5 (P=0.009), but no significant increase was observed in the lowintensity exercise group as compared to the control group (PLIN3, P=0.067 & PLIN5, P=0.44). As regards insulin resistance, there was a significant difference among the three groups (P=0.0001). Eventually, the result of the correlation between PLIN3 and PLIN5 showed similar enhancement by increasing the intensity (P=0.0026). Conclusion: According to research results, high-intensity endurance training increased the expression of PLIN3 and PLIN5 in diabetic specimens and PLIN3 and PLIN5 followed a similar increase pattern in high-intensity training

Author(s):  
Mahnaz Alinejad ◽  
Alireza Barari ◽  
Asieh Abbasi Daloii ◽  
Parvin Farzanegi

Introduction: The aim of this study was to investigate the effect of endurance training and stem cell injection on FGF2 and MMP13 gene expression in knee tissue of rats with knee osteoarthritis. Methods: The type of study was an experimental one.Male Wistar rats were randomly divided into 5 groups: control - healthy, control - patient, patient - stem cell, patient - training, and patient - training - stem cell. The training program consisted of 30 minutes of running on a treadmill with no slope at 16 m / min for the first week, with 1 m / min added weekly. Rats received MSCs through intracellular injection of 1*106 cells / kg. Expression of FGF2 and MMP13 genes was measured by Real Time PCR. One way ANOVA and if there was a significant difference, Tukey post hoc test were used to determine the difference between groups. All statistical analyzes were performed using SPSS version 20. Results: Data analysis showed that training and stem cell therapy have significantly increased in genes expression of FGF2 and MMP-13 in mice with osteoarthritis (p <0.000). Moreover, Tukey post hoc test showed a significant difference in the level of FGF2 changes in the training-stem cell groups compared to the training group and the saline group (p=0.000‌). There was also a significant difference between the MMP-13 follow-up test with the control group with the saline group, the training group and the stem cell with the train-stem cell, and the saline group with the training group9(P=0.000‌). Conclusion: The results showed that FGF2 levels and MMP13 in the cartilage tissue of mice with osteoarthritis have increased and endurance training and stem cells therapy caused a decrease in the level of factors.  


2018 ◽  
Vol 17 (2) ◽  
pp. 219-222
Author(s):  
Zhang Haimin

Long-term high-intensity training can cause a decline in immune function in athletes. In this study, we have explored whether bovine colostrum could improve the immune function in athletes undergoing intensive training. Thirty professional athletes were randomly divided into control group and bovine colostrum group; both groups received one month of intensive training. The control group received usual colostrum-free diet and the bovine colostrum group was fed bovine colostrum-rich milk every day. The venous blood was collected from both groups before and after the treatment period. The results showed that the lymphocyte population increased significantly in the bovine colostrum group compared to the control group (P < 0.05). There was no significant change in CD4+ and CD8+ in the two groups (P < 0.05), but the CD3+ and CD4+/CD8+ of the bovine colostrum group were significantly higher than those of the control group. There were significant differences in albumin, globulin and albumin/globulin ratio in the control group (P < 0.05), but not in the bovine colostrum group after training. The bovine colostrum group had lower serum albumin and albumin/globulin (P < 0.05) and higher globulin (P < 0.05) compared to the control group. There was no significant difference in immunologic indexes before and after training in both groups (P > 0.05). The decline in the markers of immune function after high-intensity training was ameliorated by the intake of bovine colostrum.


2020 ◽  
Author(s):  
Feng Liu ◽  
Gaixia Hou ◽  
Cheng Liang

Abstract Objective: This study aimed to analyze the effects of resuming training using yoga on the physical functions and fitness of Taekwondo athletes after high-intensity training.Method: A total of 50 Taekwondo athletes from this college were randomly divided into 2 groups. 25 athletes are in the control group, and they massage their muscles after high-intensity training to help recovery. Meanwhile, 25 athletes are in observation groups, and they practice yoga after high-intensity training for resuming training. After 8 weeks, the physical functions and fitness of the two groups of athletes were compared.Result: There was no statistically significant difference between the observation group and the control group before starting high-intensity training from 1st week to 8th week (P<0.05). From the 4th week to the 8th week after high-intensity training, the observation group's heart rate, heart rate after resuming training and subjective physical sensation degree scores were lower than the control group (P<0.05). After 8 weeks of resuming training in the observation group, hemoglobin was higher than the control group, blood urea nitrogen and creatine kinase was lower than the control group (P<0.05). The observation group's flexion test results of the sitting position after 4th week and 8th week of resuming training were higher than those of the control group (P<0.05).Conclusion: The use of yoga for resuming training after high-intensity training can improve the physical function and fitness of Taekwondo athletes, improve the flexibility of athletes and promote their professional skills.


Author(s):  
Nivash Rugbeer ◽  
Demitri Constantinou ◽  
Georgia Torres

Background: High-intensity training is comprised of sprint interval training (SIT) and high-intensity interval training (HIIT). This study compared high-intensity training with moderate-intensity continuous training (MICT) on cardiorespiratory fitness (CRF) and body fat percentage for overweight or obese persons. Methods: A systematic search of randomized controlled trials using the health science databases occurred up to April, 2020. Twenty-six studies were included for complete analysis. A total of 784 participations were analyzed. The unstandardized mean difference for each outcome measurement was extracted from the studies and pooled with the random effects model. Results: MICT was significantly better at improving CRF compared with SIT (mean difference = −0.92; 95% confidence interval, −1.63 to −0.21; P = .01; I2 = 10%). Furthermore, there was no significant difference between MICT versus HIIT on CRF (mean difference = −0.52; 95% confidence interval, −1.18 to 0.13; P = .12; I2 = 23%). There was no significant difference in body fat percentage between MICT versus HIIT and MICT versus SIT. Conclusions: MICT was significantly better at improving CRF than SIT in overweight or obese persons.


2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Wolfgang Kemmler ◽  
Andreas Wittke ◽  
Michael Bebenek ◽  
Michael Fröhlich ◽  
Simon von Stengel

Time-effective protocols may potentially increase people’s compliance with exercise. The purpose of this paper was to compare the relative effects of 16 weeks of high intensity (resistance) training (HIT) with and without protein supplementation (HIT&P) and HVHIT (high volume/high intensity training) versus a nontraining control group on cardiometabolic risk factors. One hundred and twenty untrained males 30–50 years old were randomly assigned to 3 subgroups: (a) a HIT group; (b) a HIT&P group, and (c) a waiting-control group (phase I) that crossed over to (d) high volume/high intensity training (HVHIT) during the second study phase. HIT was defined as “single set to failure protocol” while HVHIT consistently applied two sets. Protein supplementation provided an overall intake of 1.5 g/kg/body mass. Primary study endpoint was the metabolic syndromeZ-Score (MetS-Z-Score). MetS-Z-Score significantly improved in all exercise groups (p≤0.001) with no significant difference between HIT, HIT&P, and HVHIT (p≥0.829). However, all the exercise groups differed significantly from the CG (p<0.001) which deteriorated significantly (p=0.039). In conclusion, all exercise protocols were similarly effective in improving cardiometabolic risk factors. Thus, HIT may be the best choice for people with low time budgets looking to improve their cardiometabolic health.


2021 ◽  
Vol 27 (spe2) ◽  
pp. 66-69
Author(s):  
Lili Wang ◽  
Hongfei Wang ◽  
Wanliang Tuo

ABSTRACT At present, many studies have proved that proper exercise can promote the immune function of human body to a certain extent, but athletes need a lot of high-intensity sports training, and their immune function declines instead of improving. In order to control the decline of immune function of athletes after high-intensity training, this study propose the Zhenqi Fuzheng capsule to achieve this goal. Through experimental comparison, the parameters such as white blood cell content, immunoglobulin number, T lymphocyte, human hemoglobin content and exercise exhaustion time were detected after high-intensity training. The results showed that compared with the control group taking Zhenqi Fuzheng, the weight of those who had taken qifuzhengs capsule did not change, and the content of white blood cells, immunoglobulin, hemoglobin content and exercise time increased to a certain extent. The results showed that Zhenqi Fuzheng could inhibit the decrease of body immune function after high-intensity exercise, then accelerate the recovery of human immune function. This study is expected to enhance the immunity of sports athletes, and reduce athletes’ pain after high-intensity training.


1998 ◽  
Vol 4 (1) ◽  
pp. 49-51
Author(s):  
Cho Yim LO

LANGUAGE NOTE | Document text in English; abstract also in Chinese.The present paper attempts to discuss the relationship between the Growth Hormone and exercise intensity. Growth Hormone causes growth, enhances fat utilization and glycogen deposition, decreases use of glucose, etc. The high intensity training physical training may increase the release of Growth Hormone.本文章嘗試探討生長激素與運動訓練的關係。生長激素能促進人體生長、增加蛋白質的合成、和減低糖原的消耗。在高運動強度的訓練時,人體分泌生長激素會增加,此增長也有利蛋白質的合成。


2021 ◽  
Vol 12 ◽  
Author(s):  
Kirstie Jodie Turner ◽  
David Bruce Pyne ◽  
Julien D. Périard ◽  
Anthony John Rice

Purpose: The effects of two different high-intensity training methods on 2,000 m rowing ergometer performance were examined in a feasibility study of 24 national-level rowers aged 18–27 years (17 males, 2,000 m ergometer time trial 6:21.7 ± 0:14.6 (min:s) and seven females, 2,000 m ergometer 7:20.3 ± 0:12.1. Habitual training for all participants was ~12–16 h per week).Methods: 16 high-intensity ergometer sessions were completed across two 3-week periods. Participants were allocated into two groups according to baseline 2,000 m time. High-intensity interval session-sprint-interval session (HIIT-SIT) completed eight HIIT (8 × 2.5 min intervals; 95% of 2,000 m wattage) followed by eight SIT (three sets of 7 × 30 s intervals; maximum effort). SIT-HIIT completed eight SIT sessions followed by eight HIIT sessions. Both a 2,000-m time trial and a progressive incremental test finishing with 4 min “all-out” performance were completed before and after each 3-week phase.Results: Both groups showed similar improvements in 2,000 m time and 4 min “all-out” distance after the first 3 weeks (2,000 m time: HIIT-SIT: −2.0 ± 0.6%, mean ± 90% CL, p = 0.01; SIT-HIIT: −1.5 ± 0.3%, p = 0.01) with no significant difference between groups. HIIT-SIT demonstrated the greatest improvements in submaximal heart rate (HR) during the progressive incremental test with eight sessions of HIIT showing a greater reduction in submaximal HR than eight sessions of SIT. The net improvement of 16 high-intensity sessions on 2,000 m time was −2.5% for HIIT-SIT (−10.6 ± 3.9 s, p = 0.01) and − 2.2% for SIT-HIIT (−9.0 ± 5.7 s, p = 0.01) and for 4 min “all-out” performance was 3.1% for HIIT-SIT (36 ± 25 m, p = 0.01) and 2.8% for SIT-HIIT (33 ± 27 m, p = 0.01).Conclusion: Eight sessions of high-intensity training can improve 2,000 m ergometer rowing performance in national-level rowers, with a further eight sessions producing minimal additional improvement. The method of high-intensity training appears less important than the dose.


Author(s):  
Zohreh Shanazari ◽  
Mohammad Faramarzi ◽  
Ebrahim Banitalebi ◽  
Roohullah Hemmati

Abstract Background Skeletal muscle is very sensitive to extracellular and intracellular signaling evoked by contractions from endurance and resistance exercise. The aim of this study was to compare the effects of moderate- and high-intensity endurance and resistance training on the serum myostatin (MSTN) and insulin-like growth factor (IGF-1) levels in older rats. Materials and methods Fifty old Wistar male rats (23 months old) were randomly divided into four experimental and one control groups, including moderate-endurance training (MET) (n = 10), high-intensity endurance training (EHT) (n = 10), moderate-intensity resistance training (MRT) (n = 10), high-intensity resistance training (HRT) (n = 10), and control group (C) (n = 10). Seventy-two hours after the last exercise session, euthanasia of the rats were rendered unconscious and direct blood samples were collected. Serum IGF-1 and MSTN concentration were measured using the enzyme-linked immuno sorbent assay (ELISA) method. The statistical analysis was performed using one-way analysis of variance (ANOVA) test with a significance level of p ≤ 0.05. Results There was a significant reduction in MSTN and an increase in IGF-1 concentrations was observed between IGF-I levels in high and moderate resistance and endurance training. However, no significant difference was observed in MSTN levels between groups. Discussion Therefore, it appears that resistance training, especially HRT, is effective to increase growth mediators among older rats.


2020 ◽  
Author(s):  
Hedwig Theda Stenner ◽  
Julian Eigendorf ◽  
Arno Kerling ◽  
Momme Kück ◽  
Alexander A. Hanke ◽  
...  

Abstract Background To test the effects of guided endurance training on work ability in middle-aged female hospital workers of various occupations. Methods We randomized 265 healthy, sedentary, middle-aged women (45-65 years) to an endurance training group (EG 210 min/week) or a wait-list control group (CG). At baseline and at 6-month follow-up, we assessed work ability (Work Ability Index [WAI]), physical activity (Freiburger activity questionnaire) and peak oxygen uptake (VO 2peak ) by cardiopulmonary exercise testing. To examine the influence of baseline work ability, participants were divided into poor-moderate (WAI 1, 7-36 points, n=83), good (WAI 2, 37-43 points, n=136) and excellent (WAI 3, 44-49 points, n=46) WAI subgroups. Results Cardiorespiratory fitness improved significantly after 6 months in the EG but not in the CG. The WAI total score increased significantly in the EG (38.3±5.0 to 39.8±4.9 points) but not in the CG (39.4±4.7 to 39.3±4.9 points), with a significant difference between groups (p<0.01). In the EG, only the poor-moderate subgroup (WAI 1, 33.0±2.9 to 36.6±4.8 points, p<0.05) increased the WAI total score, with this increase being significantly higher compared to the good (WAI 2, 40.2±2.1 to, 40.4±3.7 points) and excellent (WAI 3, 45.6±1.5 to 45.7±1.8 points) subgroup. Conclusions A 6-month guided exercise training intervention significantly increases cardiorespiratory fitness with concomitant improvements in work ability in middle-aged previously sedentary hospital employees. Women with low baseline work ability seem to particularly benefit from the intervention, which implies that similar interventions may be particularly beneficial for this group of individuals.


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