scholarly journals Intratubular Enterococcus faecalis viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy

2020 ◽  
Vol 14 (4) ◽  
pp. 214-217
Author(s):  
Jamileh Ghoddusi ◽  
Ehsan Arian ◽  
Maryam Golmohammadi ◽  
Maryam Gharechahi ◽  
Siavash Moushekhian
Keyword(s):  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Sampling Procedure ◽  
T Test ◽  
Root Canals ◽  
Paired Samples ◽  
Viable Bacteria ◽  
Root Canal Instrumentation ◽  
Reciprocating Systems

Background. The present in vitro study aimed to compare the effectiveness of the WaveOne and ProTaper Gold systems in removing the Enterococcus faecalis biofilm. Methods. Thirty-eight mandibular premolars were selected. The root canals were assigned to standard control (canals serially enlarged with ProTaper Gold S1-S2-F1-F2, n=15) and experimental (canals enlarged with Primary WaveOne file, n=15) groups. Following the instrumentation procedure, the root canals underwent a sampling procedure, and the colonyforming unit (CFU) counts were determined. The samples were also evaluated under a fluorescent microscope to evaluate viable bacteria. The data were analyzed using independent samples t test and paired samples t test. Results. The results showed that, compared with the ProTaper group, the WaveOne group exhibited the least viable bacteria (P=0.004). Conclusion. It was concluded that comparison with the ProTaper Gold rotary system, the WaveOne reciprocating file is more successful in reducing intratubular viable bacteria counts.

Force and vibration analysis in biomechanical preparation of root canals using reciprocating endodontic file system: In vitro study

2021 ◽  
pp. 095441192110442
Author(s):  
Vinod Singh Thakur ◽  
Pavan Kumar Kankar ◽  
Anand Parey ◽  
Arpit Jain ◽  
Prashant Kumar Jain
Keyword(s):  
Root Canal ◽  
Vibration Analysis ◽  
File System ◽  
In Vitro Study ◽  
Root Canal Preparation ◽  
Root Canals ◽  
Improper Use ◽  
Four Levels ◽  
Root Canal Instrumentation

The shaping and cleaning of the root canal are very important in root canal treatment. The excessive force and vibration during biomechanical preparation of the root canal may result in failure of the endodontic file. In this study, force and vibration analysis was carried out during root canal preparation. The samples of human extracted (premolar) teeth were provided by the College of Dental Science and Hospital. Endodontic instruments for reciprocating motion, such as the WaveOne Gold file system, had been used for root canal preparation. Force and vibration signals were recorded by dynamometer and accelerometer, respectively. The acquired signals were denoised using the db4 (SWT denoising 1-D) wavelet. Four levels of decomposition were carried out for each signal. The signal denoising technique was used to remove unwanted noise from the acquired signal. FESEM analysis was used to visualize the levels of severity of endodontic files during the cleaning and shaping of the root canal. In most of the cases, the failure occurred due to the improper use of the root canal instrumentation. The optimum amount of force was used to avoid the file failure and provided the proper instrumentation. The curve fitting regression model was used to find the interdependency between force and vibration.

The Effectiveness of Three Instrumentation Techniques on the Elimination of Entererococcus Faecalis from a Root Canal: An In Vitro Study

2005 ◽  
Vol 6 (1) ◽  
pp. 94-106 ◽  
Author(s):  
Meltem Çolak ◽  
Sinan Evcil ◽  
Y. Ziya Bayindir ◽  
Nimet Yigit
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Bacterial Cells ◽  
Sterile Saline ◽  
Root Canals ◽  
Rotary Instruments ◽  
Colony Forming Units ◽  
Before And After

Abstract The in vitro reduction of a bacterial population in a root canal by mechanical instrumentation using three techniques was evaluated. Root canals inoculated with a Enterococcus faecalis (E. faecalis) suspension were instrumented using hand Hedstroem files, Giromatic files, and Hero 642 rotary instruments. Irrigation was performed using sterile saline solution. Root canals were sampled before and after instrumentation. After serial dilutions, samples were plated onto Mitis-Salivarius agar and the colony forming units grown were counted. All instruments tested were able to significantly reduce the number of bacterial cells in the root canal, however, the results of this study indicated that Hedstroem files, Giromatic, and Hero 642 techniques were not significantly different in their ability to reduce intracanal bacteria. Citation Colak M, Evcil S, Bayindir Y, Yigit N. The Effectiveness of Three Instrumentation Techniques on the Elimination of Enterococcus Faecalis from a Root Canal: An In Vitro Study. J Contemp Dent Pract 2005 February;(6)1:094-106.

Bactericidal Efficacy of Photodynamic Therapy and Chitosan in Root Canals Experimentally Infected with Enterococcus faecalis: An In Vitro Study

2017 ◽  
Vol 35 (4) ◽  
pp. 184-189 ◽  
Author(s):  
Fabio Camacho-Alonso ◽  
Encarnación Julián-Belmonte ◽  
Fernando Chiva-García ◽  
Yolanda Martínez-Beneyto
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canals ◽  
Bactericidal Efficacy

scholarly journals Antibacterial Effect of Endodontic Disinfections on Enterococcus Faecalis in Dental Root Canals—An In-Vitro Model Study

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2427
Author(s):  
Stefan Kranz ◽  
André Guellmar ◽  
Franziska Braeutigam ◽  
Silke Tonndorf-Martini ◽  
Markus Heyder ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Bacterial Colonization ◽  
In Vitro Study ◽  
Microbial Culture ◽  
Dentinal Tubules ◽  
Root Canals ◽  
Model Study ◽  
Chloramine T

Enterococcus faecalis (E. faecalis) is rather unsusceptible to many root canal disinfections which often cause a therapeutic problem. Therefore, the present in vitro study observed the efficiency of different endodontic antiseptics in their capability to suppress E. faecalis, especially inside dentinal tubules. Prior to any testing, root canals of extracted third human molars were inoculated with E. faecalis for 48 h. Antiseptic dressings with chloramine-T or calcium hydroxide (CaOH) for 24 h or irrigations with 1.3% sodium hypochlorite (NaOCl) were applied with n = 10 in each group. As control irrigation with normal saline was used. All treated canals were manually enlarged from size ISO 50 to 110 and the ablated dentin debris was subjected to microbial culture analysis. Bacterial colonization of the dentinal tubules up to 300 µm was verified by scanning electron microscopy and histological sample preparation. Application of crystalline chloramine-T caused total bacterial suppression inside the dentinal tubules. Dressings with CaOH showed only minor effects. Irrigation with NaOCl caused total eradication of bacteria adhering to the root canal walls, but also failed to completely suppress E. faecalis inside the dentinal tubules. The study showed that chloramine-T is of strong antiseptic activity and also efficient in suppressing E. faecalis inside dentinal tubules.

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