scholarly journals Evaluation of Genomic DNA Extraction Using Monolayer and Bilayer Magnetic Nanoparticles

2020 ◽  
Vol 8 (2) ◽  
pp. 51-54
Author(s):  
Hadiseh Rostami ◽  
Farzaneh Firoozeh ◽  
Mohammad Zibaei ◽  
Iman Salahshoorifar
Keyword(s):  
Staphylococcus Aureus ◽  
Magnetic Nanoparticles ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Clinical Isolates ◽  
Aureus Strain ◽  
Strain Atcc ◽  
Genomic Dna Extraction ◽  
General Surface

Background: There are different methods for genomic DNA extraction. Magnetic nanoparticles (MNPs) exhibit many important features making them suitable for DNA extraction. Objectives: The aim of this study was to compare the effect of monolayer and bilayer MNPs on genomic DNA extraction. Materials and Methods: The genomic DNA was extracted from the Staphylococcus aureus strain ATCC 25923 and clinical isolates using monolayer MNPs SiO2 and Fe3O4 and bilayer MNPs SiO2/Fe3O4. Then, the quality and quantity of the obtained genomic DNA were compared with both NPs. Results: The obtained results showed the concentration and purity of the extracted genomic DNA using bilayer magnetic NPs was significantly higher in comparison to the extracted genomic DNA by monolayer MNPs. Conclusion: In general, surface-coated MNPs are much more efficient than naked MNPs for genomic DNA extraction.

scholarly journals Investigating prevalence of pathogenic genes (ETA and TSST-1) in Staphylococcus aureus isolated from different wards of the hospitals by PCR method

2015 ◽  
Vol 3 (2) ◽  
pp. 239
Author(s):  
Rashid Ramazanzadeh ◽  
Hadi Mohammadi Talvar ◽  
Mahdi Mirzaii ◽  
Seyed Sajjad Hasheminasab ◽  
Hanar Narenji
Keyword(s):  
Staphylococcus Aureus ◽  
Infectious Diseases ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Blood Agar ◽  
Genomic Dna Extraction ◽  
Pathogenic Genes ◽  
Pcr Method ◽  
Pathogenic Organisms

<p>Staphylococcus aureus is the most common pathogenic organisms in the hospitals and communities' infections. It is responsible for more than 80 percent of infectious diseases. The purpose of the present paper is to determine the incidence of Staphylococcus pathogenic genes isolated from different wards of hospitals by PCR method. This study included 61 Staphylococcus aureusisolates collected from different wards hospital, between 2011 and 2012 in University of Kurdistan (Toohhid and Besat hospitals). All isolates were previously identified as Staphylococcus aureusby a standard microbiological procedure. It isolates were incubated at 37Ċ for 24h on blood agar; single colonies were tested with tube and slide coagulase, catalase tests and growth on Manito salt agar. Following genomic DNA extraction, the presence of ETA, TSST-1 genes was analyzed by PCR.61 strains of Staphylococcus aureushave been isolated from different wards of the hospital. Frequency of tst gene was 81% and eta gene was 47%. Moreover, frequency of strains with both eta and tst genes was 40%. Results of the present paper indicate that the prevalence of Staphylococcusaureus results on prevalence of eta and tst genes, and this is a matter of concern.</p>

scholarly journals One-stop genomic DNA extraction by salicylic acid-coated magnetic nanoparticles

2013 ◽  
Vol 442 (2) ◽  
pp. 249-252 ◽  
Author(s):  
Zhongwu Zhou ◽  
Ulhas S. Kadam ◽  
Joseph Irudayaraj
Keyword(s):  
Salicylic Acid ◽  
Magnetic Nanoparticles ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction ◽  
One Stop

Optimization of genomic DNA extraction from formalin-fixed fish specimens

2013 ◽  
Vol 19 (6) ◽  
pp. 1068-1073
Author(s):  
Xiaolan KONG ◽  
Zuozhi CHEN ◽  
Lin LIN ◽  
Chunhou LI ◽  
Peiwen LIANG
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction ◽  
Formalin Fixed

Optimization of genomic DNA extraction from grapevine cultivars

2012 ◽  
Vol 29 ◽  
pp. S220
Author(s):  
Karlygash Aubakirova ◽  
Madina Omasheva ◽  
Natalya Ryabushkina ◽  
Laura Yerbolova ◽  
Tolepbergen Tazhibaev ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction

scholarly journals Whole-Genome Sequence for Methicillin-Resistant Staphylococcus aureus Strain ATCC BAA-1680

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Luke T. Daum ◽  
Violet V. Bumah ◽  
Daniela S. Masson-Meyers ◽  
Manjeet Khubbar ◽  
John D. Rodriguez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Whole Genome Sequence ◽  
Aureus Strain ◽  
Whole Genome ◽  
Strain Atcc ◽  
Methicillin Resistant

scholarly journals Optimization of BY2 cell suspension as a stable transformable system

2014 ◽  
Vol 42 (2) ◽  
pp. 472-477 ◽  
Author(s):  
Zhou SHUMIN ◽  
Chu YANXIA ◽  
Zheng BANG ◽  
Zhang WEI
Keyword(s):  
Cell Suspension ◽  
Dna Extraction ◽  
Genomic Dna ◽  
35S Promoter ◽  
Gfp Expression ◽  
Genomic Dna Extraction ◽  
Agrobacterium Mediated Transformation ◽  
Culture Cells ◽  
Low Efficiency

Tobacco (Nicotiana tabacum) cv. ‘Bright Yellow 2’ (BY2) cell suspension is a useful system to study the structure and function of plant cell. However, low efficiency of Agrobacterium-mediated transformation, and transgene silencing during subculture limit its application. Here we present optimization of the traditional protocols of Agrobacterium-mediated transformation and genomic DNA extraction. The transforming efficiency and recovery ratio of genomic DNA extraction were substantially increased by these improvements. Southern assay demonstrated that copy number of transgene could be determined unambiguously. Meanwhile by monitoring the GFP fluorescence we found that the GFP expression can keep stable in suspension culture cells for at least 20 days in liquid medium. Finally, applicability of constitutive promoters of Arabidopsis thaliana UBIQUITIN10 (AtUBQ10) and ARABIDOPSISSKP1 HOMOLOGUE1 (AtASK1) also can drive stable GFP expression in vivo of BY2 cells like CaMV 35S promoter in this plant system./span>

Genomic DNA extraction from herbarium samples of Cunila D. Royen ex L. (Lamiaceae) and Polygala L. (Polygalaceae)

2010 ◽  
Vol 3 (1) ◽  
pp. 37-39 ◽  
Author(s):  
Gustavo Agostini ◽  
Raquel Lüdtke ◽  
Sergio Echeverrigaray ◽  
Tatiana Teixeira de Souz-Chies
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction
Sign in / Sign up

Export Citation Format

Share Document