scholarly journals Serum Expression of IL-33 and ST2 in Patients with Psoriasis Vulgaris

2021 ◽  
Vol 24 (9) ◽  
pp. 689-695
Author(s):  
Yonghua Dong ◽  
Hua Hu ◽  
Dandan Fu ◽  
Shuting Zheng ◽  
Qingqing Wang ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Serum Concentration ◽  
Psoriasis Vulgaris ◽  
Blood Lipid ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Expression Levels ◽  
Interleukin 33 ◽  
Immune Mediated ◽  
Biochemical Analyzer

Background: Psoriasis vulgaris (PsV) is an immune-mediated skin disease of unknown mechanism. Interleukin 33 (IL-33) is a member of IL-1 cytokine family and suppression of tumorigenicity 2 (ST2) is the specific ligand of IL-33. It has been found that IL-33 and ST2 are increased in psoriatic lesions, but the expression levels in serum and their relationship to clinical features are still unclear. The aim of this study is to assess IL-33, ST2, IL-17 and IL-5 serum levels as well as serum concentration of blood glucose and blood lipids in PsV patients and their relationship with clinical characteristics. Methods: Sixty-eight PsV samples and 60 healthy individuals were recruited. Serum levels of IL-33, ST2, IL-17 and IL-5 were measured by enzyme-linked immunosorbent assay and blood glucose and blood lipid were assayed by automatic biochemical analyzer. Results: Serum levels of IL-33, ST2, IL-17 and IL-5 were increased significantly in PsV patients compared with controls (P<0.01). Cytokines were overexpressed in PsV patients during active stages compared with controls (P<0.05). Expression levels of IL-33, ST2 and IL-17 confirmed a significance in different severity groups of PsV patients (P<0.05). Serum concentration of triglyceride (TG) was also increased compared with controls (P=0.024). IL-33 levels were positively correlated with total cholesterol (TC) levels (r=0.319, P=0.008). Conclusion: IL-33/ST2 could generally reflect the activity and disease severity in PsV patients, which indicates that the IL-33/ST2 signaling pathway plays an important role in the pathogenesis of PsV.

How matrix metalloproteinase (MMP)-9 (rs3918242) polymorphism affects MMP-9 serum concentration and associates with autism spectrum disorders: A case-control study in Iranian population

2021 ◽  
pp. 1-7
Author(s):  
Javid Rezaei Lord ◽  
Farhad Mashayekhi ◽  
Zivar Salehi
Keyword(s):  
Serum Concentration ◽  
Matrix Metalloproteinase ◽  
Serum Levels ◽  
Autism Spectrum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mmp9 Expression ◽  
Relationship Of ◽  
The Relationship ◽  
Metalloproteinase 9 ◽  
Control Study

Abstract The aim of this project was to evaluate the relationship of matrix metalloproteinase-9 (MMP-9) genetic variation and its serum concentration with autism spectrum disorder (ASD). One hundred ASD and 120 controls were enrolled in this study. Genomic DNA was extracted from blood and MMP-9 polymorphism was determined by polymerase chain reaction restriction fragment length polymorphism and serum levels were measured by enzyme-linked immunosorbent assay. The frequencies of CC, CT, and TT genotypes were 72%, 26%, and 2% in controls and 31%, 57%, and 12% in ASD, respectively. The frequencies of C and T alleles in ASD were 59.5% and 40.5%, and controls were 86% and 14%, respectively. There is a significant increase in serum MMP-9 levels in ASD as compared to controls. We have also shown that TT genotype is significantly associated with increase serum MMP-9 levels in patients (TT, CT, and CC serum levels were 91.77 ± 10.53, 70.66 ± 7.21, and 38.66 ± 5.52 and in controls were 55.55 ± 11.39, 42.66 ± 7.85, and 30.55 ± 6.34 ng/ml, respectively). It is concluded that there is a significant association between rs3918242 MMP-9 polymorphism and its serum concentration with autism. We also suggest that TT genotype is associated with increased MMP9 expression and may be a risk factor for ASD.

scholarly journals Apolipoprotein M Serum Levels Correlate with IgA Vasculitis and IgA Vasculitis Nephritis

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Jiali Wu ◽  
Lagu He ◽  
Le Bai ◽  
Li Tan ◽  
Min Hu
Keyword(s):  
Protective Factor ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Iga Vasculitis ◽  
Independent Predictive Factor ◽  
Apolipoprotein M ◽  
Biochemical Analyzer ◽  
And Gender ◽  
Study Participants

Objective. IgA vasculitis (lgAV) is the most frequent vessel vasculitis in children, and the prognosis is related to the children’s age and degree of nephritis. This study is aimed at investigating serum apolipoprotein M (apoM) levels in patients with lgAV patients and at evaluating the association between apoM and disease severity. Methods. A total of 109 lgAV patients and 76 age- and sex-matched healthy controls were included. The age and gender of the study participants were matched. ApoM levels were measured by an enzyme-linked immunosorbent assay. Additionally, the serum levels of lipids, apolipoproteins, kidney biochemical profiles, immunoglobulins (IgA, IgG, IgM, and IgE), and the complements (C3 and C4) were assessed using an automatic biochemical analyzer. Results. ApoM was increased significantly in lgAV patients compared to healthy controls. ApoM, meanwhile, was lower in patients with nephritis than in those without nephritis. The apoM levels were higher in classes I and II IgA vasculitis nephritis (lgAVN) patients than in classes III and IV. Besides, the apoM serum level<24.81 mg/L was an independent predictive factor for lgAVN and can be independently associated with the presence of nephritis in lgAV patients. Meanwhile, the serum apoM concentration negatively correlated with the ISKDC grading score in lgAVN patients. Conclusions. Serum apoM was elevated in lgAV patients and decreased gradually with the ISKDC grading score. ApoM (OR=0.32, 95%CI=0.12‐0.85, p=0.023) was identified as a protective factor for nephritis in all lgAV patients.

scholarly journals Skin tissue expression and serum level of thymic stromal lymphopoietin in patients with psoriasis vulgaris

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Oki Suwarsa ◽  
Hartati Purbo Dharmadji ◽  
Endang Sutedja ◽  
Lengga Herlina ◽  
Putri Reno Sori ◽  
...  
Keyword(s):  
Psoriasis Vulgaris ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Tissue Expression ◽  
Thymic Stromal Lymphopoietin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lesional Skin ◽  
Healthy Control ◽  
Polymerase Chain ◽  
The Difference

Thymic stromal lymphopoietin (TSLP) is known to be associated with allergic diseases. It is also suggested that TSLP has a role in autoimmune diseases such as psoriasis; however, the associated pathways remain unknown. There is currently little information on TSLP in psoriasis vulgaris. We investigated TSLP expressions on lesional and non-lesional skin of psoriasis vulgaris patients using reverse transcription- polymerase chain reaction. TSLP level was also investigated in serum from psoriasis vulgaris patients compared to healthy control using enzyme-linked immunosorbent assay. TSLP expression was higher in lesional skin (1.90) compared to non-lesional skin (1.76); however, the difference was not statistically significant (P>0.05). TSLP serum levels were significantly higher in psoriasis patients (287.40 pg/dL) as compared to controls (114.70 pg/dL) (P<0.05). This study concluded that TSLP levels in the serum of psoriasis vulgaris patients are higher than controls. TSLP was also found in keratinocyte of psoriasis patients, the expression was higher in the lesional compared to non-lesional skin; however, this difference is statistically insignificant. These findings suggest that TSLP may play a role in the pathogenesis of psoriasis vulgaris, but its exact role remains unclear.

Serum concentration of VEGF, sFLT-1, and CD105 in patients with endometrial carcinoma.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e15564-e15564
Author(s):  
Johanna Maenpaa ◽  
Sami Kristian Saarelainen ◽  
Nina Peltonen ◽  
Terho Lehtimaki ◽  
Maarit Vuento
Keyword(s):  
Serum Concentration ◽  
Endometrial Carcinoma ◽  
Preoperative Evaluation ◽  
Multivariable Analysis ◽  
Myometrial Invasion ◽  
Serum Levels ◽  
Disease Stage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Preoperative Serum ◽  
Study Results

e15564 Background: Preoperative evaluation of the risk for metastases in endometrial carcinoma is challenging. The aim of this study was to compare preoperative serum levels of angiogenetic markers VEGF, sFLT-1, and CD105 (endoglin) in predicting a metastasized disease. Methods: Preoperative sera from 100 consecutive patients diagnosed for endometrial carcinoma were collected. The serum concentrations of VEGF, sFLT-1, and CD105 were assessed by enzyme-linked immunosorbent assay (ELISA). The results were correlated to the presence of metastases, presence of deep (≥50%) myometrial invasion and histologic grade of the tumor. Cases with other than endometrioid histology were excluded from the study. Results: Eleven patients had a metastasized disease (≥Stage IIIA, FIGO 2009 classification). The serum concentration of VEGF was higher in the group with metastases (median [range] 394 pg/mL [31-1524 pg/mL] vs. 801 pg/mL [631-1183 pg/mL], p=0.001). The concentrations of sFLT-1 and CD105 did not show statistical difference between the two groups. In the multivariable analysis, the concentration of VEGF was the sole independent factor for the presence of metastases (OR 1.003, 95% CI 1.000-1.005, p=0.019). Conclusions: Preoperative serum VEGF concentration correlates with the presence of metastases in endometrioid endometrial carcinoma.

scholarly journals THU0277 THE EXPRESSION OF IFNΑ, INFΒ AND INFΓ AND SERUM LEVELS OF THOSE CYTOKINES IN SJÖGREN’S SYNDROME PATIENTS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 365.1-365
Author(s):  
M. Maślińska ◽  
A. Paradowska-Gorycka ◽  
A. Wajda ◽  
K. Kostyra-Grabczak ◽  
B. Kwiatkowska
Keyword(s):  
Serum Concentration ◽  
Rheumatoid Factor ◽  
Mrna Level ◽  
Serum Levels ◽  
Type I ◽  
Type I Ifn ◽  
Serum Concentrations ◽  
Expression Levels ◽  
Schirmer’S Test ◽  
Non Parametric

Background:In the pathogensis of autoimmune mediated diseases, such as Sjögren’s syndrome (SS), interferons (IFN) and IFN pathway activation play a vital role.Objectives:We planned to assess IFNα, INFβ and INFγ expression and IFNs serum levels in SS patients and correlation of these parameters with: autoantibodies specific for SS, serum concentration of C3, C4 component of complement (C3, C4), rheumatoid factor (RF), gammaglobulins, focus score (FS) and eye dryness symptoms.Methods:Whole blood RNA was isolated from 77 SS patients [F91%vsM9%]; mean age 49,69±15.36; SS diagnosis according to EULAR/ACR 2016 criteria. The analysis of INFα, - β and - γ expression levels was based on validated TaqMan probes by ΔCT methods. Serum concentrations of rheumatoid factor (RF), C3- and C4 complement components (mg/dL) and gammaglobulins (g/dL), were assessed. Anti-Ro/SSA and/or anti-La/SSB autoantibodies were assessed by semiquantitative immunoblotting evaluation. The eye dryness and keratoconjunctivitis sicca were confirmed with Schirmer’s test (score of less than 5 mm/5’) and the ocular staining score (OSS) using lissamine green and fluorescein staining. The biopsy of minor salivary gland was performed with the histopathological evaluation of FS. The study was approved by the Bioethics Committee. Differences between groups of patients were determined using non-parametric Mann-Whitney U test or Kruskall-Wallis test with Dunn’s post hoc. Correlations were determined using non-parametric Spearman test. The level of statistical significance was set at p < 0.05.Results:IFNβ had the highest expression levels among IFNs and IFNβ serum concentrations were higher than those of IFNα and -γ. In cases with high IFNβ serum concentration lower IFNβ expression was observed. There was a highly significant correlation between IFNα and IFNβ expression (r =0.6;p=0.001). IFNβ expression (p=0.059) was higher in the group of younger (<45 y.o.) patients (n= 23; 29.9%) as compared to the group of older individuals (at least 45 y.o.). In patients with SS-A / Ro antibodies with strong antigen binding affinity (3) IFN β expression and IFNβ serum levels were highest of all IFNs. The presence of anti La/SS-B antibodies was associated with the increased IFNβ expression while not with the increased IFNβ serum concentration. In terms of IFNα expression and protein level, RF(+)patients had average higher values compared to RF(-) patients. The average mRNA level of IFNα was about 3 times lower in patients with low C3 serum concentration compared to patients with normal C3 serum concentration values. IFNβ mRNA level was 2.5 times lower in patients with low Schirmer’s test (<5mm/5’) in comparison to patients with Schirmer’s test>5mm/5’; Schirmer’s test <5mm/5’ was associated with higher IFNβ serum concentration.Conclusion:Type I IFN signature predominates in the peripheral blood of studied patients. Presented results confirmed the pivotal role of type I IFN in the disease process. The serum concentration of IFNβ and the expression of IFNβ were the highest values of those parameters for cytokines assessed in this study. A positive correlation between IFNα and IFNβ mRNA levels has been observed.Disclosure of Interests:None declared

scholarly journals HYPOGLYCEMIC EFFECT OF HIGH-RESISTANT STARCH ANALOG RICE THROUGH GLP-1 AND INSULIN OR HIGH-RESISTANT STARCH ANALOG RICE ATTENUATES BLOOD GLUCOSE LEVEL THROUGH ENHANCEMENT OF GLP-1 AND INSULIN

2019 ◽  
pp. 172-175
Author(s):  
HAIRRUDIN ◽  
SOETJIPTO ◽  
RETNO HANDAJANI
Keyword(s):  
Blood Glucose ◽  
Resistant Starch ◽  
Serum Levels ◽  
Negative Control ◽  
Diabetic Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Treatment Groups

Objective: This study was to investigate the effect of analog rice (AR) on glucagon-like peptide-1 (GLP-1) and insulin serum levels, glucose transporter-2 (GLUT-2) expression, and fasting blood glucose (FBG) level in diabetic rats. Methods: Fifty male Wistar rats divided into the control group (n=10) and the experimental group. High-fat diet and streptozotocin were administered in experimental groups, which then divided into four equal groups (n=10, each) (negative control group, rice group, AR1 and AR2 group, given standard pellet, rice pellet, AR1 and AR2 pellet, respectively, for 6 weeks). GLP-1 and insulin serum levels were measured by enzyme-linked immunosorbent assay. The expression of GLUT-2 and the number of pancreatic β-cells observed using an immunohistochemistry method. Results: FBG levels in the AR1 and AR2 groups decreased, while the rice group remained. GLP-1 serum levels of the negative control and rice groups were not significantly different from the control group, while the AR1 and AR2 groups higher than the control group (p≤0.05). All the treatment groups had insulin serum levels significantly lower than control group (p≤0.05), except the AR1 group. The expression of GLUT-2 and the number of pancreatic β-cells in the treatment groups were less than the control group, but between treatment groups were not significantly different. Conclusion: AR significantly effective in reducing FBG level in diabetic rats through stimulation of increased GLP-1 and insulin serum levels serum levels but AR did not affect on the expression of GLUT-2.

scholarly journals 25-Hydroxyvitamin D Serum Levels Unrelated to Fasting Blood Glucose Levels of Premenopausal Women in Padang

2021 ◽  
Vol 1 (2) ◽  
pp. 46-53
Author(s):  
Junira Erasta ◽  
Delmi Sulastri ◽  
Afriwardi
Keyword(s):  
Blood Glucose ◽  
Significant Relationship ◽  
Pearson Correlation ◽  
Fasting Blood Glucose ◽  
Premenopausal Women ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

Low serum 25-Hydroxyvitamin D levels in premenopausal women result in the impaired release of insulin from the pancreas and reduce glucose tolerance which causes the body's metabolism to slow down resulting in weight gain leading to insulin resistance and resulting in diabetes mellitus (DM).The incidence of DM occurs at premenopausal ages compared to productive ages, the percentage of women experiencing diabetes is higher than men. This study aims to determine the relationship between 25-Hydroxyvitamin D serum levels and fasting blood glucose (FBG) levels of premenopausal women in Padang. Method: This study was conducted in the city of Padang, is observational, cross-sectional design. The study sample was 62 premenopausal women. Measurement of serum 25-Hydroxyvitamin D levels was measured by enzyme-linked immunosorbent assay and FBG levels were measured by the GOD-PAP method. Data were analyzed using the Pearson correlation test. Results: The average serum level of 25-Hydroxyvitamin D respondents was 30.96 ± 10.96 ng/ml. The average FBG level of respondents was 107.03 ± 13.74 mg/dl. There was no significant relationship between 25-Hydroxyvitamin D serum levels and FBG levels (r = -0.038, p = 0.769). Conclusion: There is no significant relationship between 25-Hydroxyvitamin D serum levels and FBG levels of premenopausal women in Padang.

scholarly journals Upregulation of the miRNA-155, miRNA-210, and miRNA-20b in psoriasis patients and their relation to IL-17

2020 ◽  
Vol 34 ◽  
pp. 205873842093374 ◽  
Author(s):  
Mohamed El-Komy ◽  
Iman Amin ◽  
Marwa Safwat El-Hawary ◽  
Dina Saadi ◽  
Olfat Shaker
Keyword(s):  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epigenetic Mechanisms ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Lesional Skin ◽  
Immune Mediated ◽  
Elisa Technique ◽  
Polymerase Chain ◽  
The Relationship

Psoriasis is an immune-mediated disease, with genetic background and triggering environmental factors; however, several gaps are still present in understanding the intertwined relationship between these elements. Epigenetic mechanisms, including microRNAs (miRNAs), play an important role in the pathogenesis of psoriasis. The relationship between interleukin (IL)-17, a key cytokine in psoriasis, and these epigenetic mechanisms still needs to be elucidated. This study aimed at assessing the expression of miRNA-155, miRNA-210, and miRNA-20b in skin and sera of psoriasis patients in relation to IL-17 levels. For 20 psoriasis patients and 20 matching controls, the expression of miRNA-155, miRNA-210, and miRNA-20b was assessed using real-time polymerase chain reaction (RT-PCR), whereas IL-17/IL-17A levels were measured using quantitative enzyme-linked immunosorbent assay (ELISA) technique. MiRNA-155 expression was significantly higher in lesional skin compared to controls ( P = 0.001). MiRNA-210 expression was significantly higher in both, lesional skin ( P = 0.010) and sera of patients ( P = 0.001) in comparison with controls. A statistically significant positive correlation was found between serum miRNA-210 expression and serum levels of IL-17/IL-17A ( P = 0.010, rs = 0.562). MiRNA-20b lesional and non-lesional expression was significantly higher than controls ( P < 0.001; P = 0.018). In conclusion, the expression of miRNA-155, miRNA-210, and miRNA-20b is exaggerated in psoriasis and they may be involved in disease pathogenesis. A possible relationship between miRNA-210 and IL-17 may be suggested; however, further studies are still needed to verify this relation.

scholarly journals The Difference in Interleukin-19 Serum on Degrees of Acne Vulgaris Severity

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Moerbono Mochtar ◽  
Alamanda Murasmita ◽  
M. Eko Irawanto ◽  
Indah Julianto ◽  
Harijono Kariosentono ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Acne Vulgaris ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Severe Acne ◽  
Significant Difference ◽  
The Difference ◽  
Degrees Of Severity

Introduction. Acne vulgaris is a multifactorial disease. Recent study showed that inflammation does have a central role in the formation of both inflammatory and noninflammatory lesions in acne vulgaris. There are various findings of proinflammatory cytokines related to acne vulgaris, but no previous study correlate interleukin- (IL-) 19 to acne vulgaris. This pilot study aims to look at difference in IL-19 serum concentration on degrees of severity of acne vulgaris. Methods. This is an analytical observational cross-sectional study. Sample subjects were patients with acne vulgaris who met the inclusion criteria. Enzyme-linked immunosorbent assay (ELISA) study was applied to measure IL-19 serum. Result. Analysis test found statistically significant difference between IL-19 serum concentration of group of patients with mild acne vulgaris and that of group of patients with severe acne vulgaris. Moreover, analysis revealed significant difference between IL-19 serum concentration of group of patients with moderate acne vulgaris and that of group of patients with severe acne vulgaris. Conclusions. There are differences in serum levels of IL-19 on the severity of acne vulgaris. The significant difference might show that inflammation has a core role in severity of acne vulgaris, and IL-19 might potentially be related to acne vulgaris.

The Relationship of Serum Hemojuvelin and Hepcidin Levels with Iron Overload in Nonalcoholic Fatty Liver Disease

2015 ◽  
Vol 24 (3) ◽  
pp. 293-300 ◽  
Author(s):  
Salih Boga ◽  
Huseyin Alkim ◽  
Canan Alkim ◽  
Ali Riza Koksal ◽  
Mehmet Bayram ◽  
...  
Keyword(s):  
Liver Disease ◽  
Iron Overload ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Fatty Liver Disease ◽  
Clinical Characteristics ◽  
Serum Levels ◽  
Fibrosis Stage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nonalcoholic Fatty Liver

Background & Aims: Mild iron overload is frequently reported in patients with nonalcoholic fatty liver disease (NAFLD). Hepcidin is the master iron-regulatory peptide and hemojuvelin (HJV) is the key regulator of iron-dependent secretion of hepcidin. The aims of this study were to evaluate serum HJV and hepcidin levels in patients with biopsy-proven NAFLD with and without hepatic iron overload, and to identify potential associations of HJV and hepcidin with the clinical characteristics of the patients enrolled. Methods: Serum levels of HJV and hepcidin were measured in 66 NAFLD patients with (n=12) and without (n=54) iron overload, and controls (n=35) by enzyme-linked immunosorbent assay. Hemojuvelin and hepcidin levels were assessed in relation to clinical characteristics and liver histologic evaluation of the participants. Results: Significantly lower serum HJV (281.1 [239.2-353.6] vs. 584.8 [440.3-661] ng/ml, p<0.001) and similar serum hepcidin levels (60.5±31.1 vs. 55.8±11.9 ng/ml, p=0.285) were found in NAFLD patients when compared to controls. İron-overloaded NAFLD patients had significantly lower HJV (249.9 [187.6-296.3] vs. 292.9 [243-435] ng/ml, p=0.032) and significantly higher hepcidin (78.4±35.5 vs. 56.5±28.9ng/ml, p=0.027) levels than NAFLD patients without iron overload. Fibrosis stage was significantly higher in iron overloaded NAFLD group (p<0.001). Ferritin levels correlated significantly both with HOMA-IR (r=0.368, p=0.002) and fibrosis stage (r=0.571, p<0.001). Conclusions: Our findings suggest that HJV levels are low in NAFLD and even lower in iron overloaded NAFLD, while hepcidin levels are higher in NAFLD with iron overload. The gradually decreased HJV and increased hepcidin concentrations in our patients most likely reflect the physiological response to iron accumulation in the liver.

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