scholarly journals Novel Synergistic Activity of Quercus infectoria Gall Extract with Ceftazidime Against Standard and Multiple Drug Resistant Pseudomonas aeruginosa and Escherichia coli Isolates

2021 ◽  
Vol 24 (9) ◽  
pp. 684-688
Author(s):  
Elham Isaei ◽  
Shahla Mansouri ◽  
Maryam Rahmani ◽  
Fariba Sharififar ◽  
Atefeh Salary
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Synergistic Activity ◽  
Bacterial Strains ◽  
Frap Assay ◽  
E Coli ◽  
Quercus Infectoria ◽  
Checker Board

Background: Multidrug resistance pathogens are important heath challenges. In this study, the antibacterial activity of 20 plant extracts was tested against standard as well as 20 multidrug-resistant (MDR) strains of Pseudomonas aeruginosa and Escherichia coli. The most active plant extract (Quercus infectoria) was selected for the synergistic activity assay. Methods: Plant extracts were prepared by maceration using water, methanol and ethanol. The antibacterial activity of extracts was determined by both broth and agar dilution methods. The synergistic activity of QIG with ceftazidime (CAZ) was evaluated by checker board assay. Antioxidant activity was determined by colorimetric Ferric reducing antioxidant power (FRAP) assay. Results: Only the methanol extract of QIG inhibited the growth of all the bacterial strains at a concentration of 1000 µg/mL. Other active extracts were Myrtus communis and Eucalyptus globulus inhibiting the growth of most bacterial strains tested at 2000 µg/ mL. In checker board assay, the minimum inhibitory concentration (MIC) to both QIG extract and CAZ was reduced. The MIC of CAZ was reduced from 64-4096 µg/mL to 4 µg/mL for P. aeruginosa and to 16 µg/mL for E. coli isolates. Conclusion: The QIG extract exhibited potent antioxidant activity determined by FRAP assay. The result of this study showed a strong synergistic activity between QIC and CAZ on P. aeruginosa and E. coli. The activity within ethyl acetate-methanol (7:3) fraction indicates that the active components of the plant have a semi-polar nature and further work with this fraction may lead to understanding the mechanism of this synergistic activity.

scholarly journals FLORAL EXTRACT OF Tibouchina granulosa (DESR.) COGN. PHYTOCHEMISTRY PROSPECTION, AND ANTIBACTERIAL ACTIVITY

2021 ◽  
Vol 5 (1) ◽  
pp. 1-9
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Matheus Vinícius Abadia Ventura ◽  
Carlos Frederico de Souza Castro
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Floral Organ ◽  
Salt Formation ◽  
Bacterial Strains ◽  
Positive Results

Tibouchina granulosa is a species that blooms annually in several regions of Brazil. This species is still little explored in terms of phytocompounds in all organs of this plant, especially the floral organ. Flowers of T. granulosa were collected in the municipality of Rio Verde, Goiás, Brazil, in 2021. The hydroethanolic floral extract was prepared by maceration and qualitative phytochemical (colorimetric reactions and salt formation) and antibacterial analyzes performed and the results expressed in millimeters of inhibition at different concentrations in mg mL-1. Several phytochemical classes were observed with positive results, especially for alkaloids, phenolics, oxylates, saponins, carbohydrates and tannins. As for the bacterial assay, potential antibacterial activity was observed for all bacterial strains tested, except for Salmonella serovar Thyphymurium and serovar Enteritidis. Expressive inhibitions were observed for Enterococcus faecalis > Pseudomonas aeruginosa > Staphylococcus aureus and Escherichia coli at the highest concentrations between 100-50 mg mL-1. The floral extract of Tibouchina granulosa showed phytotherapeutic potential with the presence of several phytochemical groups and expressive antibacterial activity.

scholarly journals Phytochemical screening and synergistic interactions between aminoglycosides, selected antibiotics and extracts from the bryophyte Octoblepharum albidum Hedw (Calymperaceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 465-470 ◽  
Author(s):  
C.A.S. Vidal ◽  
E.O. Sousa ◽  
Fabíola Rodrigues ◽  
Adriana Campos ◽  
S.R. Lacerda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Synergistic Effect ◽  
Ethanolic Extract ◽  
Antibiotic Activity ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
E Coli ◽  
Synergistic Interactions

This work is the first to describe the modulation of antibiotic activity of the bryophyte Octoblepharum albidum Hedw extract. The antibacterial activity of ethanolic extract of O. albidum (EEOa), alone and in association with aminoglycosides, was determined against six bacterial strains by a microdilution test. The results showed a similar inhibitory activity of EEOa against Escherichia coli ATCC 25922 and Klebsiella pneumoniae ATCC 33018 (MICs 512 ?g/mL). The synergistic effect of the extracts and aminoglycosides was also verified. The most pronounced effects were obtained with EEOa + gentamicin against E. coli and EEOa + kanamycin against K. pneumoniae with MICs reduction (128 to 32 ?g/mL). The data from this study are indicative of the antibacterial activity of the bryophyte O. albidum extracts and its potential in modifying the resistance of aminoglycosides analyzed.

Antibacterial Activities of Semipurified Fractions of Quercus infectoria against Enterohemorrhagic Escherichia coli O157:H7 and Its Verocytotoxin Production

2008 ◽  
Vol 71 (6) ◽  
pp. 1223-1227 ◽  
Author(s):  
SUPAYANG PIYAWAN VORAVUTHIKUNCHAI ◽  
SAKOL SUWALAK
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Latex Agglutination ◽  
Enterohemorrhagic Escherichia Coli ◽  
Natural Food ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Broth Microdilution Method ◽  
Quercus Infectoria

Escherichia coli O157:H7 is one of the most important foodborne pathogens, causing nonbloody and bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. Use of antibiotics has been demonstrated to result in increased levels of verocytotoxin (VT) production as well as antibiotic resistance. Quercus infectoria was investigated for its antibacterial activity against E. coli O157:H7 and other VT-producing enterohemorrhagic E. coli (VTEC). The MIC was determined by a broth microdilution method, and the MBC was assessed by subculturing the bacteria from the wells that showed no apparent growth onto Mueller-Hinton agar. The fractions Qi2, Qi3, and Qi4 of Q. infectoria were demonstrated to possess good antibacterial activity, with MICs and MBCs ranging from 250 to 500 μg/ml. The effect of the effective fraction, Qi4, on the production of VT was determined using a reversed passive latex agglutination. The results indicate that at 20 h, fraction Qi4 markedly inhibits the release of VT1 and VT2 from VTEC cells at both inhibitory and subinhibitory concentrations. Furthermore, verotoxicity assay demonstrated that bacterial cultures treated with fraction Qi4 exerted less toxic effect on Vero cells. These in vitro results clearly indicate that the fraction Qi4 might constitute a promising natural food additive for the control of food poisoning by E. coli O157:H7 as well as other VTEC strains.

scholarly journals Synthesis and biological evaluation of some new 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines as antimicrobial agents

2022 ◽  
Vol 11 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Iryna Myrko ◽  
Taras Chaban ◽  
Vasyl Matiychuk
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Bacterial Strains ◽  
Synthesis And Biological Evaluation

A series of some new pyrazole-substituted 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines was synthesized in this study. The structures of target substances were confirmed by using 1H and 13С NMR spectroscopy, mass spectrometry and elemental analysis. The synthesized compounds have been evaluated for antimicrobial activity against five bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus) and two fungal strains (Candida albicans and Cryptococcus neoformans). The antimicrobial screening studies of synthesized substances established that 2 of 12 compounds show pronounced antibacterial activity against the strain Staphylococcus aureus.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Sarang Lebah Hutan (Apis Dorsata) Terhadap Pertumbuhan Staphylococcus aureus, Escherichia coli Dan Pseudomonas aeruginosa

2018 ◽  
Vol 14 (1) ◽  
pp. 179
Author(s):  
Ika Prestianti ◽  
Maswati Baharuddin ◽  
Sappewali Sappewali
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Apis Dorsata ◽  
Soaking Time ◽  
E Coli ◽  
Extract Concentration ◽  
X 24 ◽  
Paper Disc

<p>Penyakit infeksi akibat bakteri merupakan masalah serius dalam kesehatan. Antibakteri alami yang dapat digunakan untuk menghambat pertumbuhan bakteri yaitu sarang lebah hutan (<em>Apis dorsata</em>) (propolis, kantong madu, kantong telur dan kantong pollen) yang mengandung senyawa metabolit sekunder seperti flavonoid, tanin dan asam fenolat.Tujuan dari penelitian ini adalah megetahui aktivitas antibakteri ekstrak sarang lebah hutan dari setiap pelarut yang digunakan dan untuk mengetahui pengaruh konsentrasi ekstrak sarang lebah hutan terhadap pertumbuhan bakteri <em>Staphylococcus aureus, Escherichia coli </em>dan<em> Pseudomonas aeruginosa</em>. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram dengan lama perendaman   1 jam kemudian diinkubasi selama 3 x 24 jam. Hasil penelitian menunjukkan bahwa aktivitas antibakteri ekstrak metanol sarang lebah memiliki aktivitas tertinggi pada bakteri  <em>E. coli </em>yaitu 3,8 mm pada konsentrasi 8%, ekstrak etil asetat sarang lebah pada bakteri <em>S. a ureus </em>yaitu 3,72 mm pada konsentrasi 8% dan ekstrak n-heksan sarang lebah pada bakteri <em>E. coli </em>yaitu 16,1 mm pada konsentrasi 8%. Pengaruh konsentrasi ekstrak yaitu semakin tinggi konsentrasi ekstrak maka semakin besar pula daya hambat ekstrak terhadap pertumbuhan bakteri <em>S</em>.<em> aureus, E</em>.<em> coli </em>dan<em> P</em>.<em> aeruginosa</em>.</p><p><strong>Antibacterial Evaluation of Extract Beehive (<em>Apis dorsata</em>) on Growth <em>Staphylococcus aureus</em>, <em>Escherichia coli</em> and <em>Pseudomonas aeruginosa</em></strong>. Bacterial infectious diseases are serious health problems. One of natural antibacterial that can be used to inhibit bacterial growth is honeycomb (propolis, honey bag, egg bag and pollen bag) containing secondary metabolite compounds such as flavonoids, tannins and phenolic acids. The purpose of this research is to know the antibacterial activity of honeycomb extract from each solvent used and to know the effect of honeycomb extract concentration on the growth of <em>S</em>.<em> aureus</em>, <em>E</em>. <em>coli</em> and <em>P</em>.<em> aeruginosa</em> bacteria. Antibacterial testing was performed by the method of paper disc diffusion with soaking time 1 hour then incubated for 3 x 24 hours. The results showed that antibacterial activity of methanol honeycomb extract had the highest activity in <em>E. coli</em> bacteria i.e., 3.8 mm at 8% concentration, ethyl acetate honey extract on <em>S. aureus</em> bacteria ie 3.72 mm at 8% concentration and n extract n-hexan honeycomb in <em>E. coli</em> bacteria that is 16,1 mm at 8% concentration. The higher extract concentration the greater the inhibitory activity of extract on the growth of <em>S</em>.<em> aureus</em>, <em>E. coli</em> and <em>P</em><em>.</em><em> aeruginosa</em> bacteria.</p>

scholarly journals Invitro antibacterial activity of Cassia fistula Linn methanolic leaf extracts

2019 ◽  
Vol 8 (2) ◽  
pp. 270
Author(s):  
Bindhu R. Kamath ◽  
Sabeena Kizhedath
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cassia Fistula ◽  
Leaf Extracts ◽  
E Coli

Background: Cassia fistula Linn is a plant which is widely grown in India and is used for medicinal purposes. The study was carried out with an objective to demonstrate the antimicrobial activity of leaves of Cassia fistula Linn. The aim of the study is to assess antibacterial and antifungal activity of methanolic leaf extract of Cassia fistula Linn against selected clinical isolates.Methods: The antimicrobial activity of methanolic extract of Cassia fistula was evaluated using agar well diffusion method and to zone of inhibition of extract was determined. Clinical isolates of Staphyloccocus aureus, MRSA, Pseudomonas aeruginosa, E. coli and Proteus were screened.Results: The methanolic extracts exhibited antibacterial activity against Staphylococcus aureus. The extract was not active against E. coli, Proteus, MRSA, Pseudomonas aeruginosa. The extract also failed to demonstrate antifungal activity against Candida albicans and Aspergillus niger.Conclusions: The global emergence of multidrug resistant bacterial strains is increasing, limiting the effectiveness of current drugs and treatment failure of infections. A novel approach to the prevention of antibiotic resistance of pathogenic species is the use of new compounds that are not based on existing synthetic antimicrobial agents.

scholarly journals Evaluation of Antibacterial Activity of Three Quaternary Ammonium Disinfectants on Different Germs Isolated from the Hospital Environment

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Amal Ramzi ◽  
Bouchra Oumokhtar ◽  
Yassine Ez zoubi ◽  
Touria Filali Mouatassem ◽  
Moussa Benboubker ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Quaternary Ammonium ◽  
Inhibitory Concentration ◽  
Hospital Environment ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli

Background. The microbiological risk of the hospital environment, including inert surfaces, medical devices, and equipment, represents a real problem. Objective. This study is aimed at demonstrating and assessing the antibacterial activity of three synthetic disinfectants classified as quaternary ammoniums on different bacterial strains (Gram-negative and Gram-positive like Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus) isolated from the hospital environment. The reference strains included Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853 used as negative control strains. Method. Three quaternary ammonium disinfectants were tested: DDN9® (0.5%) which contains didecylmethylpolyoxyethylammonium propionate as an active substance, spray (0.4%) containing quaternary ammonium compounds, and Phagosurf ND® (0.4%) with didecyldimethylammonium chloride. Their effect was evaluated using the disk diffusion technique and the broth dilution methods, allowing the Minimum Inhibitory Concentration (MIC) and then the Minimum Bactericidal Concentration (MBC). Result. Only the growth of Gram-positive bacteria and some strains of Gram-negative bacteria were inhibited by the three synthetic disinfectants. NDD9® demonstrated an antibacterial effect only against the Gram-positive strains (S. aureus and S. aureus ATCC 29213) with a MIC of 0.25 mg/ml. The disinfectant spray showed effect against all four strains including E. coli (9), S. aureus, E. coli ATCC 25922, and P. aeruginosa ATCC 27853 with an inhibitory concentration of 4 mg/ml, while the growth of S. aureus ATCC 29213 was inhibited at 2 mg/ml. The third disinfectant, Phagosurf ND®, inhibited only the growth of S. aureus ATCC 29213 at a MIC of 4 mg/ml. Conclusion. This study is the first here in Morocco to evaluate the bacterial activity of products intended for the control of the healthcare environment. The results obtained on the three disinfectants tested reveal an ineffectiveness against some isolated strains from the hospital environment.

scholarly journals Towards a new treatment against polymicrobial infections: high antibacterial activity of lemon IntegroPectin against Pseudomonas aeruginosa and Escherichia coli

2020 ◽  
Author(s):  
Alessandro Presentato ◽  
Antonino Scurria ◽  
Lorenzo Albanese ◽  
Pasquale Picone ◽  
Mario Pagliaro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Industrial Development ◽  
Antimicrobial Treatment ◽  
Hydrodynamic Cavitation ◽  
E Coli ◽  
High Antibacterial Activity ◽  
New Treatment ◽  
Polymicrobial Infections

AbstractLemon IntegroPectin obtained via hydrodynamic cavitation of waste lemon peel in water only shows high antibacterial activity against two Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli. The antibacterial effect against the ubiquitous pathogen P. aeruginosa was evaluated in terms of the minimal bactericidal (MBC) and minimal inhibitory concentration (MIC). Preliminary insight on the antibacterial mechanism of IntegroPectin originates from investigating its inhibitory activity against E. coli. Given the non-cytotoxic nature of citrus IntegroPectin and the ease of its reproducible production in large amounts, the route is open to the industrial development of a new antimicrobial treatment against polymicrobial infections unlikely to develop drug resistance.

scholarly journals KHẢO SÁT ĐIỀU KIỆN NUÔI CẤY ĐỂ NÂNG CAO KHẢ NĂNG KHÁNG KHUẨN CỦA CÁC CHỦNG Streptomyces sp.

2021 ◽  
Vol 5 (1) ◽  
pp. 2227-2236
Author(s):  
Nguyễn Thị Thủy Tiên ◽  
Lê Thị Thu Hiền ◽  
Trần Thị Lệ Ngân ◽  
Trần Văn Trung ◽  
Nguyễn Thị Thúc ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
16S Rrna ◽  
Bacillus Cereus ◽  
Salmonella Typhi ◽  
Streptomyces Sp ◽  
International Streptomyces Project ◽  
E Coli

Streptomyces là những vi sinh vật có khả năng sinh tổng hợp các chất kháng sinh. Nghiên cứu này nhằm sàng lọc và chọn chủng có khả năng kháng khuẩn cao nhất từ 59 chủng Streptomyces có nguồn gốc từ đất đã được cung cấp dựa trên phương pháp cấy vạch vuông góc đối với 5 vi khuẩn gây bệnh chỉ thị, bao gồm Bacillus cereus, Escherichia coli, Salmonella typhi, Staphylococcus aureus và Pseudomonas aeruginosa. Mười lăm chủng thể hiện hoạt tính kháng khuẩn ở các mức độ khác nhau, các chủng còn lại không thể hiện khả năng kháng. Chủng có khả năng kháng khuẩn cao nhất là HĐM3.2, kháng 4/5 vi khuẩn chỉ thị đã sử dụng, gồm B. cerius, S. aureus, S. typhi và E. coli với kích thước vùng kháng đạt 9,5, 10,5, 16,5 và 14,5 mm. Chủng HĐM3.2 được xác định là chủng Streptomyces sp. dựa trên cây phát sinh loài đã xây dựng của trình tự gene 16S rRNA. Điều kiện nuôi cấy để chủng HĐM3.2 thể hiện hoạt tính kháng khuẩn cao nhất đã được khảo sát dựa trên phương pháp khuếch tán qua giếng thạch. Môi trường International Streptomyces Project 2 có pH 8, nhiệt độ nuôi cấy 28oC là điều kiện thích hợp để chủng Streptomyces sp. HĐM3.2 tạo ra vùng ức chế các loại vi khuẩn B. cereus, E. coli, S. typhi, S. aureus cao nhất. ABSTRACT Streptomyces is the microorganism that has capable of producing antibiotics. The current study aimed to screen and to select a strain that had the highest antibacterial activity from 59 available soil-derived Streptomyces strains based on the perpendicular culture method on 5 indicator pathogenic bacteria, including Bacillus cereus, Escherichia coli, Salmonella typhi, Staphylococcus aureus và Pseudomonas aeruginosa. Fourteen strains exhibited their antibacterial activity at various levels, the remaining did not have that activity. The strain that had the highest antibacterial ability was HĐM3.2 against 4/5 indicator microorganisms, including B. cerius, S. aureus, S. typhi and E. coli with inhibitory areas were 9.5, 10.5, 16.5, and 14.5 mm respectively. Strain HĐM3.2 was identified as strain Streptomyces sp. based on a phylogenetic tree built on the 16S rRNA gene sequences. Suitable conditions that made the strain HĐM3.2 showed the highest antibacterial activity were investigated based on agar well diffusion assay. The medium of International Streptomyces Project 2 with pH 8, the ambient temperature at 28oC were suitable conditions for Streptomyces sp. HĐM3.2 produced the highest inhibitory areas against B. cereus, E. coli, S. typhi, S. aureus.  

scholarly journals Chemical Profile, Antibacterial Activity and Antibiotic-Modulating Effect of the Hexanic Zea Mays L. Silk Extract (Poaceae)

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
Ana Carvalho ◽  
Cleciana Cruz ◽  
Cicero Freitas ◽  
José Aguiar ◽  
Paula Nunes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Zea Mays ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Chemical Constituents ◽  
Chemical Profile ◽  
E Coli

The present study aimed to determine the chemical profile and to evaluate the antibacterial activity and antibiotic-modulating action of the hexanic Zea mays silk extract in association with aminoglycosides. Standard Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 strains, as well as multi-resistant Escherichia coli 27, Staphylococcus aureus 35, and Pseudomonas aeruginosa 31 strains, were used in this study. Phytochemical prospection revealed the presence of the following secondary metabolites: tannins, flavones, flavonoids, and xanthones, with the main chemical constituents being identified in plant extracts obtained with apolar organic solvents such as hexane. The extract presented a minimum inhibitory concentration (MIC) ≥1024 μg/mL against all the tested strains. The association of the extract with aminoglycoside antibiotics showed significant synergistic effects against Staphylococcus aureus and Pseudomonas aeruginosa, except for amikacin, which was antagonized by the extract against E. coli. These results indicate the Zea mays silk presents bioactive compounds with antibiotic-modulating properties. However, further research is required to characterize the effects of isolated compounds and determine their potential for drug development.

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