scholarly journals Deep Membrane Proteome Profiling of Rat Hippocampus in Simulated Complex Space Environment by SWATH

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Yun Wang ◽  
Peibin Qin ◽  
Jie Hong ◽  
Nuomin Li ◽  
Yongqian Zhang ◽  
...  
Keyword(s):  
Circadian Rhythms ◽  
Complex Space ◽  
Field Tests ◽  
Underlying Mechanism ◽  
Nervous System Development ◽  
Rat Hippocampus ◽  
Space Environment ◽  
Space Biology ◽  
Membrane Proteome

Despite the development and great progress in the field of space biology, the astronauts are still facing many challenges in space. The space environment in which astronauts stay includes microgravity, noise, circadian rhythms disorder, and confinement, which has deep effect both on the physiology and psychology of astronauts. It was reported that long-term flight could cause the astronauts’ anxiety and depression. However, the underlying mechanism is not yet fully understood. Therefore, in the present study, the rat tail suspension model with noise, circadian rhythms, and confinement was employed to simulate complex space environment. We found that the rats exhibited the depressive-like behavior by the sucrose preference, forced swimming, and open-field tests. The membrane proteome of the rat hippocampus was investigated by “SWATH quantitation” technology both in control and simulated complex space environment (SCSE) groups. Out of 4520 quantified proteins, 244 differentially expressed membrane proteins were obtained between the SCSE and control rats, which were functionally enriched in a series of biological processes, such as translation, protein phosphorylation, brain development, endocytosis, nervous system development, axonogenesis, and vesicle-mediated transport. We found a reduction level of neurexin-2, the light, medium, heavy polypeptide of neurofilament, rab 18, synaptogyrin 1, and syntaxin-1A and an increase level of neuroligin-1, munc18, snapin, synaptotagmin XII, complexin-1, etc., which may play a key part in the development of depression. Furthermore, GSK-3β protein was upregulated in mass spectrometry, which was further validated by western blotting. The results of the study do the favor in designing the effective countermeasures for the astronauts in the future long-term spaceflight.

Wnt pathway regulation by long-term moderate exercise in rat hippocampus

2014 ◽  
Vol 1543 ◽  
pp. 38-48 ◽  
Author(s):  
S. Bayod ◽  
I. Menella ◽  
S. Sanchez-Roige ◽  
J.F. Lalanza ◽  
R.M. Escorihuela ◽  
...  
Keyword(s):  
Wnt Pathway ◽  
Rat Hippocampus ◽  
Moderate Exercise ◽  
Pathway Regulation

scholarly journals Not BCL2 mutation but dominant mutation conversation contributed to acquired venetoclax resistance in acute myeloid leukemia

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Xiang Zhang ◽  
Jiejing Qian ◽  
Huafeng Wang ◽  
Yungui Wang ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Underlying Mechanism ◽  
Lymphocytic Leukemia ◽  
First Line ◽  
Dominant Mutation ◽  
First Line Therapy ◽  
Line Therapy ◽  
Acute Myeloid

AbstractVenetoclax (VEN) plus azacitidine has become the first-line therapy for elderly patients with acute myeloid leukemia (AML), and has a complete remission (CR) plus CR with incomplete recovery of hemogram rate of ≥70%. However, the 3-year survival rate of these patients is < 40% due to relapse caused by acquired VEN resistance, and this remains the greatest obstacle for the maintenance of long-term remission in VEN-sensitive patients. The underlying mechanism of acquired VEN resistance in AML remains largely unknown. Therefore, in the current study, nine AML patients with acquired VEN resistance were retrospectively analyzed. Our results showed that the known VEN resistance-associated BCL2 mutation was not present in our cohort, indicating that, in contrast to chronic lymphocytic leukemia, this BCL2 mutation is dispensable for acquired VEN resistance in AML. Instead, we found that reconstructed existing mutations, especially dominant mutation conversion (e.g., expanded FLT3-ITD), rather than newly emerged mutations (e.g., TP53 mutation), mainly contributed to VEN resistance in AML. According to our results, the combination of precise mutational monitoring and advanced interventions with targeted therapy or chemotherapy are potential strategies to prevent and even overcome acquired VEN resistance in AML.

Effect of long-term treatment with L-deprenyl on the age-dependent microanatomical changes in the rat hippocampus

1995 ◽  
Vol 79 (2-3) ◽  
pp. 169-185 ◽  
Author(s):  
Yong-Chun Zeng ◽  
Stefano Bongrani ◽  
Elena Bronzetti ◽  
Sandro Cadel ◽  
Alberto Ricci ◽  
...  
Keyword(s):  
Term Treatment ◽  
Rat Hippocampus ◽  
Long Term Treatment ◽  
Age Dependent

Long-term cholinergic denervation caused by early postnatal AF64A lesion prevents development of Muscarinic receptors in rat hippocampus

1992 ◽  
Vol 5 (2) ◽  
pp. 131-141 ◽  
Author(s):  
Paul G.M. Luiten ◽  
Eddy A. Van der Zee ◽  
Erzsebet Gáspár ◽  
Bauke Buwalda ◽  
A.Donny Strosberg ◽  
...  
Keyword(s):  
Muscarinic Receptors ◽  
Rat Hippocampus

Orphanin FQ inhibits synaptic transmission and long-term potentiation in rat hippocampus

Hippocampus ◽  
1997 ◽  
Vol 7 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Tzu-Ping Yu ◽  
Jeffrey Fein ◽  
Tien Phan ◽  
Christopher J. Evans ◽  
Cui-Wei Xie
Keyword(s):  
Synaptic Transmission ◽  
Long Term Potentiation ◽  
Rat Hippocampus ◽  
Orphanin Fq ◽  
Term Potentiation

scholarly journals The Impact of Long-Term Exposure to Space Environment on Adult Mammalian Organisms: A Study on Mouse Thyroid and Testis

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e35418 ◽  
Author(s):  
Maria Angela Masini ◽  
Elisabetta Albi ◽  
Cristina Barmo ◽  
Tommaso Bonfiglio ◽  
Lara Bruni ◽  
...  
Keyword(s):  
Space Environment ◽  
The Impact ◽  
Mouse Thyroid

Subjective Age and Late-Life Functional Status: Mediating and Moderating Effects

2021 ◽  
Author(s):  
Joel R Petashnick ◽  
Amit Shrira ◽  
Yaakov Hoffman ◽  
Yuval Palgi ◽  
Gitit Kavé ◽  
...  
Keyword(s):  
Older Adults ◽  
Depressive Symptoms ◽  
Functional Status ◽  
Objective Measure ◽  
Later Life ◽  
Underlying Mechanism ◽  
Integrative Model ◽  
Subjective Age ◽  
Longitudinal Relationships

Abstract Objectives The present study examined the longitudinal relationships between subjective age (SA) and future functional status in later life, via depressive symptoms. Additionally, we assessed the role of subjective nearness to death (SNtD) as a potential moderator within these pathways. Methods Older adults (average age 81.14 at T1) were interviewed once a year for three consecutive years (N=224 at T1, N=178 at T2, and N=164 at T3), Participants reported their SA, SNtD, depressive symptoms, and functional status. Additionally, grip strength was employed as an objective measure of functional status. Results Data analysis revealed distinct pathways leading from T1 SA to T3 functional status through T2 depressive symptoms. Moreover, T1 SNtD was found to significantly moderate most of these indirect pathways, so that the mediation model of T1 SA-T2 depressive symptoms-T3 functional status was mostly significant among those who felt closer to death. Discussion The findings contribute to our understanding of the underlying mechanism through which SA predicts long-term functioning sequelae by underscoring the indirect effect of depressive symptoms. They further indicate the importance of gauging the effects of SNtD on these longitudinal relationships. Present results may further contribute to establishing an integrative model for predicting long-term functional outcomes based on older adults' earlier subjective views of aging.

High Holding Power Torpedo Pile: Results for the First Long Term Application

2004 ◽  
Author(s):  
Jairo Bastos de Araujo ◽  
Roge´rio Diniz Machado ◽  
Cipriano Jose de Medeiros Junior
Keyword(s):  
Water Depth ◽  
Deep Water ◽  
Field Tests ◽  
Free Fall ◽  
Mooring System ◽  
Holding Power ◽  
Campos Basin ◽  
Anchoring System ◽  
Very High

Petrobras developed a new kind of anchoring device known as Torpedo. This is a steel pile of appropriate weight and shape that is launched in a free fall procedure to be used as fixed anchoring point by any type of floating unit. There are two Torpedoes, T-43 and T-98 weighing 43 and 98 metric tons respectively. On October 2002 T-43 was tested offshore Brazil in Campos Basin. The successful results approved and certified by Bureau Veritas, and the need for a feasible anchoring system for new Petrobras Units in deep water fields of Campos Basin led to the development of a Torpedo with High Holding Power. Petrobras FPSO P-50, a VLCC that is being converted with a spread-mooring configuration will be installed in Albacora Leste field in the second semester of 2004. Its mooring analysis showed that the required holding power for the mooring system would be very high. Drag embedment anchors option would require four big Anchor Handling Vessels for anchor tensioning operations at 1400 m water depth. For this purpose T-98 was designed and its field tests were completed in April 2003. This paper discusses T-98 design, building, tests and ABS certification for FPSO P-50.

Thermoelectric Applications as Related to Biomedical Engineering for Nasa Johnson Space Center

1997 ◽  
Vol 478 ◽  
Author(s):  
C. D. Kramer ◽  
P.E.
Keyword(s):  
International Space Station ◽  
Space Station ◽  
Space Environment ◽  
Space Applications ◽  
International Space ◽  
Future Technology ◽  
Technological Developments ◽  
Environment Noise ◽  
Scientific Value

AbstractThis paper presents current NASA biomedical developments and applications using thermoelectrics. Discussion will include future technology enhancements that would be most beneficial to the application of thermoelectric technology.A great deal of thermoelectric applications have focused on electronic cooling. As with all technological developments within NASA, if the application cannot be related to the average consumer, the technology will not be mass-produced and widely available to the public (a key to research and development expenditures and thermoelectric companies). Included are discussions of thermoelectric applications to cool astronauts during launch and reentry. The earth-based applications, or spin-offs, include such innovations as tank and race car driver cooling, to cooling infants with high temperatures, as well as, the prevention of hair loss during chemotherapy. In order to preserve the scientific value of metabolic samples during long-term space missions, cooling is required to enable scientific studies. Results of one such study should provide a better understanding of osteoporosis and may lead to a possible cure for the disease.In the space environment, noise has to be kept to a minimum. In long-term space applications such as the International Space Station, thermoelectric technology provides the acoustic relief and the reliability for food, as well as, scientific refrigeration/freezers. Applications and future needs are discussed as NASA moves closer to a continued space presence in Mir, International Space Station, and Lunar-Mars Exploration.

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