scholarly journals Quantitative method for live bacteria in murine peritoneal macrophages by solubilizing with Triton X-100.

1986 ◽  
Vol 41 (3) ◽  
pp. 619-621 ◽  
Author(s):  
Haruo MATSUMURA ◽  
Yasuhiko TERADA ◽  
Masayasu NAKANO
Keyword(s):  
Quantitative Method ◽  
Peritoneal Macrophages ◽  
Live Bacteria ◽  
Murine Peritoneal Macrophages ◽  
Triton X

scholarly journals Identification of cytoskeletal components involved in attachment of L929 cells and macrophages to polystyrene.

1981 ◽  
Vol 89 (3) ◽  
pp. 691-694 ◽  
Author(s):  
K W Lanks ◽  
N W Chin
Keyword(s):  
Molecular Weight ◽  
Peptide Mapping ◽  
Limited Proteolysis ◽  
Detailed Examination ◽  
Peritoneal Macrophages ◽  
Intimate Contact ◽  
L929 Cells ◽  
Molecular Weights ◽  
Murine Peritoneal Macrophages ◽  
Triton X

We have previously shown that lactoperoxidase (LPO) covalently coupled to polystyrene tissue culture flasks can be used to radioiodinate monolayer cell proteins that come into intimate contact with the LPO-polystyrene surface. These studies have now been extended to include a detailed examination of the class of iodinated polypeptides migrating with apparent molecular weights of 50,000 and 55,000 in SDS polyacrylamide gels. Whereas in cultured L929 cells the 55,000 band is predominantly iodinated, in thioglycollate-activated murine peritoneal macrophages the 55,000 and 50,000 bands are of equal intensity. It is possible that the marked degree of exposure of the 50,000 mol wt polypeptide to immobilized LPO is related to the unique strength of macrophages attachment. After labeling of both L929 cells and macrophages with immobilized LPO, all polypeptides in this molecular weight region were subjected to peptide mapping by simultaneous limited proteolysis and electrophoresis in a second SDS polyacrylamide slab gel. The results clearly show that the two major polypeptides in this region are identical within the limits of resolution of this technique. The 55,000 mol wt polypeptide can also be identified in Triton X-100 cytoskeletons from L929 cells after labeling with soluble LPO either before or after detergent lysis. We conclude that this cell surface polypeptide is in continuity with the cytoskeleton and is preferentially exposed to the substratum during attachment to polystyrene.

Swainsonine Modulation of Protein Kinase C Activity in Murine Peritoneal Macrophages

1990 ◽  
Vol 2 (10) ◽  
pp. 333-338 ◽  
Author(s):  
Pascal Breton ◽  
Amha Asseffa ◽  
Krzysztof Grzegorzewski ◽  
Steven K. Akiyama ◽  
Sandra L. White ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Peritoneal Macrophages ◽  
Kinase C ◽  
Protein Kinase C Activity ◽  
Murine Peritoneal Macrophages

scholarly journals Differential Bacterial Survival, Replication, and Apoptosis-Inducing Ability of Salmonella Serovars within Human and Murine Macrophages

2000 ◽  
Vol 68 (3) ◽  
pp. 1005-1013 ◽  
Author(s):  
William R. Schwan ◽  
Xiao-Zhe Huang ◽  
Lan Hu ◽  
Dennis J. Kopecko
Keyword(s):  
Typhoid Fever ◽  
Systemic Infection ◽  
Peritoneal Macrophages ◽  
Human Macrophage ◽  
U937 Cells ◽  
Bacterial Survival ◽  
Human Macrophages ◽  
Macrophage Cells ◽  
Serovar Typhimurium ◽  
Murine Peritoneal Macrophages

ABSTRACT Salmonella serovars are associated with human diseases that range from mild gastroenteritis to host-disseminated enteric fever. Human infections by Salmonella enterica serovar Typhi can lead to typhoid fever, but this serovar does not typically cause disease in mice or other animals. In contrast, S. enterica serovar Typhimurium and S. entericaserovar Enteritidis, which are usually linked to localized gastroenteritis in humans and some animal species, elicit a systemic infection in mice. To better understand these observations, multiple strains of each of several chosen serovars of Salmonellawere tested for the ability in the nonopsonized state to enter, survive, and replicate within human macrophage cells (U937 and elutriated primary cells) compared with murine macrophage cells (J774A.1 and primary peritoneal cells); in addition, death of the infected macrophages was monitored. The serovar Typhimurium strains all demonstrated enhanced survival within J774A.1 cells and murine peritoneal macrophages, compared with the significant, almost 100-fold declines in viable counts noted for serovar Typhi strains. Viable counts for serovar Enteritidis either matched the level of serovar Typhi (J774A.1 macrophages) or were comparable to counts for serovar Typhimurium (murine peritoneal macrophages). Apoptosis was significantly higher in J774A.1 cells infected with serovar Typhimurium strain LT2 compared to serovar Typhi strain Ty2. On the other hand, serovar Typhi survived at a level up to 100-fold higher in elutriated human macrophages and 2- to 3-fold higher in U937 cells compared to the serovar Typhimurium and Enteritidis strains tested. Despite the differential multiplication of serovar Typhi during infection of U937 cells, serovar Typhi caused significantly less apoptosis than infections with serovar Typhimurium. These observations indicate variability in intramacrophage survival and host cytotoxicity among the various serovars and are the first to show differences in the apoptotic response of distinctSalmonella serovars residing in human macrophage cells. These studies suggest that nonopsonized serovar Typhimurium enters, multiplies within, and causes considerable, acute death of macrophages, leading to a highly virulent infection in mice (resulting in death within 14 days). In striking contrast, nonopsonized serovar Typhi survives silently and chronically within human macrophages, causing little cell death, which allows for intrahost dissemination and typhoid fever (low host mortality). The type of disease associated with any particular serovar of Salmonellais linked to the ability of that serovar both to persist within and to elicit damage in a specific host's macrophage cells.

Activation of murine peritoneal macrophages by Salmonella typhimurium

1989 ◽  
Vol 26 (1-2) ◽  
pp. 208-210
Author(s):  
J. A. M. Langermans ◽  
P. H. Nibbering ◽  
R. Furth
Keyword(s):  
Salmonella Typhimurium ◽  
Peritoneal Macrophages ◽  
Murine Peritoneal Macrophages

scholarly journals Infection with Mycobacterium aviumDifferentially Regulates the Expression of Iron Transport Protein mRNA in Murine Peritoneal Macrophages

2001 ◽  
Vol 69 (11) ◽  
pp. 6618-6624 ◽  
Author(s):  
Wangjian Zhong ◽  
William P. Lafuse ◽  
Bruce S. Zwilling
Keyword(s):  
Host Defense ◽  
Transferrin Receptor ◽  
Iron Transport ◽  
Peritoneal Macrophages ◽  
Natural Resistance ◽  
Intracellular Pathogens ◽  
Mrna Levels ◽  
Iron Availability ◽  
Iron Transporter ◽  
Murine Peritoneal Macrophages

ABSTRACT Iron is an important element for the growth of microorganisms as well as in the defense of the host by serving as a catalyst for the generation of free radicals via the Fenton/Haber-Weiss reactions. The iron transporter natural resistance-associated macrophage protein 1 (Nramp1) confers resistance to the growth of a variety of intracellular pathogens including Mycobacterium avium. Recently several other proteins that are involved in iron transport, including the highly homologous iron transporter Nramp2 and the transferrin receptor-associated protein HFE (hereditary hemochromatosis protein), have been described. The relationship of these proteins to host defense and to the growth of intracellular pathogens is not known. Here, we report that infection with M. avium differentially regulates mRNA expression of the proteins associated with iron transport in murine peritoneal macrophages. Both Nramp1 and Nramp2 mRNA levels increase following infection, while the expression of transferrin receptor mRNA decreases. The level of expression of HFE mRNA remains unchanged. The difference in the expression of the mRNA of these proteins following infection or cytokine stimulation suggests that they may play an important role in host defense by maintaining a delicate balance between iron availability for host defense and at the same time limiting iron availability for microbial growth.

Streptococcus anginosus induced nitric oxide synthesis by murine peritoneal macrophages without help of IFNγ

2005 ◽  
Vol 1284 ◽  
pp. 203-204
Author(s):  
C. Yamaura ◽  
M. Sasaki ◽  
Y. Ohara-Nemoto ◽  
S. Tajika ◽  
Y. Shimoyama ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Peritoneal Macrophages ◽  
Nitric Oxide Synthesis ◽  
Streptococcus Anginosus ◽  
Murine Peritoneal Macrophages
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