Faculty Opinions recommendation of A protease inhibitor against acute stress-induced visceral hypersensitivity and paracellular permeability in rats.

2012 ◽  
Author(s):  
Eamonn Quigley
Keyword(s):  
Protease Inhibitor ◽  
Acute Stress ◽  
Paracellular Permeability ◽  
Visceral Hypersensitivity

A protease inhibitor against acute stress-induced visceral hypersensitivity and paracellular permeability in rats

2011 ◽  
Vol 654 (3) ◽  
pp. 289-294 ◽  
Author(s):  
Juhui Zhao ◽  
Jinhai Wang ◽  
Lei Dong ◽  
Hongyang Shi ◽  
Zongyan Wang ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Acute Stress ◽  
Paracellular Permeability ◽  
Visceral Hypersensitivity

scholarly journals Local Colonic Administration of a Serine Protease Inhibitor Improves Post-Inflammatory Visceral Hypersensitivity in Rats

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 811
Author(s):  
Nikita Hanning ◽  
Michelle De bruyn ◽  
Hannah Ceuleers ◽  
Tim Boogaerts ◽  
Maya Berg ◽  
...  
Keyword(s):  
Abdominal Pain ◽  
Protease Inhibitor ◽  
Serine Protease ◽  
Serine Protease Inhibitor ◽  
Visceral Hypersensitivity ◽  
Pharmacokinetic Parameters ◽  
Trinitrobenzene Sulfonic Acid ◽  
Colonic Tissue ◽  
Inflammatory Phase

Dysregulation of the protease–antiprotease balance in the gastrointestinal tract has been suggested as a mechanism underlying visceral hypersensitivity in conditions such as inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). We aimed to study the potential therapeutic role of an intracolonically administered serine protease inhibitor for the treatment of abdominal pain in a post-inflammatory rat model for IBS. An enema containing 2,4,6-trinitrobenzene sulfonic acid (TNBS) was used to induce colitis in male Sprague–Dawley rats, whereas controls received a saline solution. Colonoscopies were performed to confirm colitis and follow-up mucosal healing. In the post-inflammatory phase, the serine protease inhibitor UAMC-00050 (0.1–5 mg/kg) or its vehicle alone (5% DMSO in H2O) was administered in the colon. Thirty minutes later, visceral mechanosensitivity to colorectal distensions was quantified by visceromotor responses (VMRs) and local effects on colonic compliance and inflammatory parameters were assessed. Specific proteolytic activities in fecal and colonic samples were measured using fluorogenic substrates. Pharmacokinetic parameters were evaluated using bioanalytical measurements with liquid chromatography–tandem mass spectrometry. Post-inflammatory rats had increased trypsin-like activity in colonic tissue and elevated elastase-like activity in fecal samples compared to controls. Treatment with UAMC-00050 decreased trypsin-like activity in colonic tissue of post-colitis animals. Pharmacokinetic experiments revealed that UAMC-00050 acted locally, being taken up in the bloodstream only minimally after administration. Local administration of UAMC-00050 normalized visceral hypersensitivity. These results support the role of serine proteases in the pathophysiology of visceral pain and the potential of locally administered serine protease inhibitors as clinically relevant therapeutics for the treatment of IBS patients with abdominal pain.

scholarly journals Amitriptyline modifies the visceral hypersensitivity response to acute stress in the irritable bowel syndrome

2009 ◽  
Vol 29 (5) ◽  
pp. 552-560 ◽  
Author(s):  
N. M. THOUA ◽  
C. D. R. MURRAY ◽  
W. J. WINCHESTER ◽  
A. J. ROY ◽  
M. C. L. PITCHER ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Acute Stress ◽  
Visceral Hypersensitivity ◽  
Hypersensitivity Response ◽  
Irritable Bowel

Acute stress-induced hypersensitivity to colonic distension depends upon increase in paracellular permeability: role of myosin light chain kinase

Pain ◽  
2005 ◽  
Vol 113 (1) ◽  
pp. 141-147 ◽  
Author(s):  
Afifa Ait-Belgnaoui ◽  
Sylvie Bradesi ◽  
Jean Fioramonti ◽  
Vassilia Theodorou ◽  
Lionel Bueno
Keyword(s):  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Acute Stress ◽  
Paracellular Permeability ◽  
Colonic Distension

IFNγ and mast cells are responsible for delayed changes in colonic paracellular permeability induced by acute stress

2003 ◽  
Vol 124 (4) ◽  
pp. A318
Author(s):  
Demaude Julien ◽  
Laurent Ferrier ◽  
Nicolas Cenac ◽  
Rafael Garcia-Villar ◽  
Jean Fioramonti ◽  
...  
Keyword(s):  
Mast Cells ◽  
Acute Stress ◽  
Paracellular Permeability
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