Faculty Opinions recommendation of The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis.

2008 ◽  
Author(s):  
Kim Lewis ◽  
Danuta Tomkiewicz
Keyword(s):  
Multidrug Resistance ◽  
Bile Acid ◽  
Lactococcus Lactis ◽  
Acid Resistance ◽  
Multidrug Resistance Transporter

scholarly journals The ABC-Type Multidrug Resistance Transporter LmrCD Is Responsible for an Extrusion-Based Mechanism of Bile Acid Resistance in Lactococcus lactis

2008 ◽  
Vol 190 (22) ◽  
pp. 7357-7366 ◽  
Author(s):  
Arsalan Haseeb Zaidi ◽  
Patrick J. Bakkes ◽  
Jacek Lubelski ◽  
Herfita Agustiandari ◽  
Oscar P. Kuipers ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Bile Acid ◽  
Lactococcus Lactis ◽  
Stress Responses ◽  
Prolonged Exposure ◽  
Morphological Changes ◽  
Cell Envelope ◽  
Acquired Resistance ◽  
Acid Resistance ◽  
Elevated Expression

ABSTRACT Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluate the contribution of efflux-based mechanisms in this process, the drug-sensitive L. lactis NZ9000 ΔlmrCD strain was challenged with cholate. A resistant strain was obtained that, compared to the parental strain, showed (i) significantly improved resistance toward several bile acids but not to drugs, (ii) morphological changes, and (iii) an altered susceptibility to antimicrobial peptides. Transcriptome and transport analyses suggest that the acquired resistance is unrelated to elevated transport activity but, instead, results from a multitude of stress responses, changes to the cell envelope, and metabolic changes. In contrast, wild-type cells induce the expression of lmrCD upon exposure to cholate, whereupon the cholate is actively extruded from the cells. Together, these data suggest a central role for an efflux-based mechanism in bile acid resistance and implicate LmrCD as the main system responsible in L. lactis.

scholarly journals LmrCD is a major multidrug resistance transporter in Lactococcus lactis

2006 ◽  
Vol 61 (3) ◽  
pp. 771-781 ◽  
Author(s):  
Jacek Lubelski ◽  
Anne de Jong ◽  
Ronald van Merkerk ◽  
Herfita Agustiandari ◽  
Oscar P. Kuipers ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Lactococcus Lactis ◽  
Multidrug Resistance Transporter

scholarly journals Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis

Genetika ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 197-206 ◽  
Author(s):  
Brankica Filipic ◽  
Branko Jovcic ◽  
Gordana Uzelac ◽  
Marija Miljkovic ◽  
Jelena Antic-Stankovic ◽  
...  
Keyword(s):  
Growth Rate ◽  
Multidrug Resistance ◽  
Lactococcus Lactis ◽  
Starter Culture ◽  
Growth Curves ◽  
Host Bacterium ◽  
Control Strain ◽  
Toxic Compounds ◽  
Over Expression ◽  
Multidrug Resistance Transporter

The influence of the over-expression of CmbT multidrug resistance transporter on the growth rate of Lactococcus lactis NZ9000 was studied. L. lactis is a lactic acid bacteria (LAB) widely used as a starter culture in dairy industry. Recently characterized CmbT MDR transporter in L. lactis confers resistance to a wide variety of toxic compounds as well as to some clinically relevant antibiotics. In this study, the cmbT gene was over-expressed in the strain L. lactis NZ9000 in the presence of nisin inducer. Over-expression of the cmbT gene in L. lactis NZ9000 was followed by RT-PCR. The obtained results showed that the cmbT gene was successfully over-expressed by addition of sub-inhibitory amounts of nisin. Growth curves of L. lactis NZ9000/pCT50 over-expressing the cmbT gene and L. lactis NZ9000 control strain were followed in the rich medium as well as in the chemically defined medium in the presence solely of methionine (0.084 mM) or mix of methionine and cysteine (8.4 mM and 8.2 mM, respectively). Resulting doubling times revealed that L. lactis NZ9000/pCT50 had higher growth rate comparing to the control strain. This could be a consequence of the CmbT efflux activity, which improves the fitness of the host bacterium through the elimination of toxic compounds from the cell.

The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis

2013 ◽  
Vol 164 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Brankica Filipic ◽  
Natasa Golic ◽  
Branko Jovcic ◽  
Maja Tolinacki ◽  
Denice C. Bay ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Lactococcus Lactis ◽  
Major Facilitator ◽  
Multidrug Resistance Transporter

scholarly journals Cholate-Stimulated Biofilm Formation by Lactococcus lactis Cells

2011 ◽  
Vol 77 (8) ◽  
pp. 2602-2610 ◽  
Author(s):  
Arsalan Haseeb Zaidi ◽  
Patrick J. Bakkes ◽  
Bastiaan P. Krom ◽  
Henny C. van der Mei ◽  
Arnold J. M. Driessen
Keyword(s):  
Bile Acid ◽  
Cell Surface ◽  
Lactococcus Lactis ◽  
Biofilm Formation ◽  
Prolonged Exposure ◽  
Resistance Mechanism ◽  
Acid Resistance ◽  
High Tendency ◽  
Surface Hydrophilicity ◽  
Content Type

ABSTRACTBile acid resistance byLactococcus lactisdepends on the ABC-type multidrug transporter LmrCD. Upon deletion of thelmrCDgenes, cells can reacquire bile acid resistance upon prolonged exposure to cholate, yielding the ΔlmrCDrstrain. The resistance mechanism in this strain is non-transporter based. Instead, cells show a high tendency to flocculate, suggesting cell surface alterations. Contact angle measurements demonstrate that the ΔlmrCDrcells are equipped with an increased cell surface hydrophilicity compared to those of the parental and wild-type strains, while the surface hydrophilicity is reduced in the presence of cholate. ΔlmrCDrcells are poor in biofilm formation on a hydrophobic polystyrene surface, but in the presence of subinhibitory concentrations of cholate, biofilm formation is strongly stimulated. Biofilm cells show an enhanced extracellular polymeric substance production and are highly resistant to bile acids. These data suggest that non-transporter-based cholate resistance inL. lactisis due to alterations in the cell surface that stimulate cells to form resistant biofilms.

scholarly journals Interaction of prenylcysteine methyl esters with the multidrug resistance transporter.

1994 ◽  
Vol 269 (23) ◽  
pp. 15973-15976
Author(s):  
L. Zhang ◽  
C.W. Sachs ◽  
R.L. Fine ◽  
P.J. Casey
Keyword(s):  
Multidrug Resistance ◽  
Methyl Esters ◽  
Multidrug Resistance Transporter

735 Multidrug Resistance Transporter Pgp as a Pathogenetic Factor of Non-small Cell Lung Cancer

2012 ◽  
Vol 48 ◽  
pp. S175
Author(s):  
T.A. Bogush ◽  
E.A. Dudko ◽  
M.V. Tikchomirov ◽  
A.B. Ravcheeva ◽  
B.E. Polotsky ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Multidrug Resistance ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Pathogenetic Factor ◽  
Multidrug Resistance Transporter

Effects of ABCB1 (multidrug resistance transporter) gene mutations on disposition and central nervous effects of loperamide in healthy volunteers

2003 ◽  
Vol 13 (11) ◽  
pp. 651-660 ◽  
Author(s):  
Carsten Skarke ◽  
Marwan Jarrar ◽  
Helmut Schmidt ◽  
Gerold Kauert ◽  
Michael Langer ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Healthy Volunteers ◽  
Gene Mutations ◽  
Transporter Gene ◽  
Multidrug Resistance Transporter
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