Faculty Opinions recommendation of Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome.

2004 ◽  
Author(s):  
Thomas Blumenthal
Keyword(s):  
Oikopleura Dioica ◽  
Spliced Leader ◽  
Trans Splicing ◽  
Spliced Leader Rna

scholarly journals Spliced-Leader RNA trans Splicing in a Chordate, Oikopleura dioica, with a Compact Genome

2004 ◽  
Vol 24 (17) ◽  
pp. 7795-7805 ◽  
Author(s):  
Philippe Ganot ◽  
Torben Kallesøe ◽  
Richard Reinhardt ◽  
Daniel Chourrout ◽  
Eric M. Thompson
Keyword(s):  
Caenorhabditis Elegans ◽  
Regulatory Elements ◽  
Sm Proteins ◽  
Oikopleura Dioica ◽  
Spliced Leader ◽  
Trans Splicing ◽  
Spliced Leader Rna

ABSTRACT trans splicing of a spliced-leader RNA (SL RNA) to the 5′ ends of mRNAs has been shown to have a limited and sporadic distribution among eukaryotes. Within metazoans, only nematodes are known to process polycistronic pre-mRNAs, produced from operon units of transcription, into mature monocistronic mRNAs via an SL RNA trans-splicing mechanism. Here we demonstrate that a chordate with a highly compact genome, Oikopleura dioica, now joins Caenorhabditis elegans in coupling trans splicing with processing of polycistronic transcipts. We identified a single SL RNA which associates with Sm proteins and has a trimethyl guanosine cap structure reminiscent of spliceosomal snRNPs. The same SL RNA, estimated to be trans-spliced to at least 25% of O. dioica mRNAs, is used for the processing of both isolated or first cistrons and downstream cistrons in a polycistronic precursor. Remarkably, intercistronic regions in O. dioica are far more reduced than those in either nematodes or kinetoplastids, implying minimal cis-regulatory elements for coupling of 3′-end formation and trans splicing.

scholarly journals The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP.

1990 ◽  
Vol 9 (11) ◽  
pp. 3667-3673 ◽  
Author(s):  
P. A. Maroney ◽  
G. J. Hannon ◽  
J. A. Denker ◽  
T. W. Nilsen
Keyword(s):  
Spliced Leader ◽  
Trans Splicing ◽  
In Trans ◽  
Spliced Leader Rna

scholarly journals Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR

2018 ◽  
Author(s):  
Gemma Danks ◽  
Heloisa Galbiati ◽  
Martina Raasholm ◽  
Yamila N. Torres Cleuren ◽  
Eivind Valen ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Growth Regulator ◽  
Translational Control ◽  
Oikopleura Dioica ◽  
Spliced Leader ◽  
Animal Density ◽  
Genome Wide ◽  
Trans Splicing ◽  
Initial Recovery ◽  
Favorable Conditions

AbstractIn phylogenetically diverse organisms, the 5’ ends of a subset of mRNAs are trans-spliced with a spliced leader (SL) RNA. The functions of SL trans-splicing, however, remain largely enigmatic. Here, we quantified translation genome-wide in the marine chordate, Oikopleura dioica, under inhibition of mTOR, a central growth regulator. Translation of trans-spliced TOP mRNAs was suppressed, showing that the SL sequence permits nutrient-dependent translational control of growth-related mRNAs. Under crowded, nutrient-limiting conditions, O. dioica continues to filter-feed, but arrests growth until favorable conditions return. Upon release from such conditions, initial recovery was independent of nutrient-responsive, trans-spliced genes, suggesting animal density sensing as a first trigger for resumption of development. Our results demonstrate a role for trans-splicing in the coordinated translational down-regulation of nutrient-responsive genes under limiting conditions.

scholarly journals Spliced leader RNA trans-splicing in dinoflagellates

2007 ◽  
Vol 104 (11) ◽  
pp. 4618-4623 ◽  
Author(s):  
H. Zhang ◽  
Y. Hou ◽  
L. Miranda ◽  
D. A. Campbell ◽  
N. R. Sturm ◽  
...  
Keyword(s):  
Spliced Leader ◽  
Trans Splicing ◽  
Spliced Leader Rna

trans-spliced Caenorhabditis elegans mRNAs retain trimethylguanosine caps

1990 ◽  
Vol 10 (4) ◽  
pp. 1764-1768
Author(s):  
R F Liou ◽  
T Blumenthal
Keyword(s):  
Caenorhabditis Elegans ◽  
Antibody Binding ◽  
Small Nuclear Rna ◽  
Nematode Caenorhabditis Elegans ◽  
Spliced Leader ◽  
C Elegans ◽  
Distinct Subset ◽  
Trans Splicing ◽  
Nuclear Rna ◽  
Spliced Leader Rna

The nematode Caenorhabditis elegans has an unusual small nuclear RNA, containing a 100-nucleotide RNA molecule, spliced leader RNA, which donates its 5' 22 nucleotides to a variety of recipient RNAs by a trans-splicing reaction. The spliced leader RNA has a 5' trimethylguanosine (TMG) cap, which becomes the 5' end of trans-spliced mRNAs. We found that mature trans-spliced mRNAs were immunoprecipitable with anti-TMG cap antibodies and that TMG-containing dinucleotides specifically competed with the trans-spliced mRNAs for antibody binding. We also found that these mRNAs retained their TMG caps throughout development and that the TMG-capped mRNAs were polysome associated. Since the large majority of C. elegans mRNAs are not trans-spliced, the addition of the spliced leader and its TMG cap to a limited group of recipient RNAs may create a functionally distinct subset of mRNAs.

scholarly journals Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Gemma B. Danks ◽  
Heloisa Galbiati ◽  
Martina Raasholm ◽  
Yamila N. Torres Cleuren ◽  
Eivind Valen ◽  
...  
Keyword(s):  
Growth Regulator ◽  
Translational Control ◽  
Oikopleura Dioica ◽  
Spliced Leader ◽  
Animal Density ◽  
Genome Wide ◽  
Trans Splicing ◽  
Initial Recovery ◽  
Favorable Conditions

Abstract Background In phylogenetically diverse organisms, the 5′ ends of a subset of mRNAs are trans-spliced with a spliced leader (SL) RNA. The functions of SL trans-splicing, however, remain largely enigmatic. Results We quantified translation genome-wide in the marine chordate, Oikopleura dioica, under inhibition of mTOR, a central growth regulator. Translation of trans-spliced TOP mRNAs was suppressed, consistent with a role of the SL sequence in nutrient-dependent translational control of growth-related mRNAs. Under crowded, nutrient-limiting conditions, O. dioica continued to filter-feed, but arrested growth until favorable conditions returned. Upon release from unfavorable conditions, initial recovery was independent of nutrient-responsive, trans-spliced genes, suggesting animal density sensing as a first trigger for resumption of development. Conclusion Our results are consistent with a proposed role of trans-splicing in the coordinated translational down-regulation of nutrient-responsive genes under growth-limiting conditions.

The evolution of spliced leader trans-splicing in nematodes

2010 ◽  
Vol 38 (4) ◽  
pp. 1125-1130 ◽  
Author(s):  
Jonathan Pettitt ◽  
Neale Harrison ◽  
Ian Stansfield ◽  
Bernadette Connolly ◽  
Berndt Müller
Keyword(s):  
Caenorhabditis Elegans ◽  
Common Ancestor ◽  
Model Organism ◽  
Spliced Leader ◽  
Trans Splicing ◽  
Rna Genes ◽  
Spliced Leader Rna

Spliced leader trans-splicing occurs in many primitive eukaryotes including nematodes. Most of our knowledge of trans-splicing in nematodes stems from the model organism Caenorhabditis elegans and relatives, and from work with Ascaris. Our investigation of spliced leader trans-splicing in distantly related Dorylaimia nematodes indicates that spliced-leader trans-splicing arose before the nematode phylum and suggests that the spliced leader RNA gene complements in extant nematodes have evolved from a common ancestor with a diverse set of spliced leader RNA genes.

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