Faculty Opinions recommendation of Allele-specific expression analysis by RNA-FISH demonstrates preferential maternal expression of UBE3A and imprint maintenance within 15q11- q13 duplications.

2002 ◽  
Author(s):  
L. Alison McInnes
Keyword(s):  
Expression Analysis ◽  
Specific Expression ◽  
Allele Specific Expression ◽  
Maternal Expression ◽  
Rna Fish ◽  
Allele Specific

scholarly journals Genome-Wide Regulatory Interactions in the Early Stages of Drosophila Speciation

2020 ◽  
Author(s):  
◽  
Alwyn Clark Go
Keyword(s):  
Reproductive Isolation ◽  
Expression Analysis ◽  
Regulatory Elements ◽  
Specific Expression ◽  
Allele Specific Expression ◽  
Regulatory Interactions ◽  
Early Stages ◽  
Genome Wide ◽  
Allele Specific ◽  
Incomplete Reproductive Isolation

Speciation occurs when reproductive barriers prevent the exchange of genetic information between individuals. A common form of reproductive barrier between species capable of interbreeding is hybrid sterility. Genomic incompatibilities between the divergent genomes of different species contribute to a reduction in hybrid fitness. These incompatibilities continue to accumulate after speciation, therefore, young divergent taxa with incomplete reproductive isolation are important in understating the genetics leading to speciation. Here, I use two Drosophila subspecies pairs. The first is D. willistoni consisting of D. w. willistoni and D. w. winge. The second subspecies pair is D. pseudoobscura, which is composed of D. p. pseudoobscura and D. p. bogotana. Both subspecies pairs are at the early stages of speciation and show incomplete reproductive isolation through unidirectional hybrid male sterility. In this thesis, I performed an exploratory survey of genome-wide expression analysis using RNA-sequencing on D. willistoni and determined the extent of regulatory divergence between the subspecies using allele-specific expression analysis. I found that misexpressed genes showed a degree of tissue specificity and that the sterile male hybrids had a higher proportion of misexpressed genes in the testes relative to the fertile hybrids. The analysis of regulatory divergence between this subspecies pair found a large (66-70%) proportion of genes with conserved regulatory elements. Of the genes showing evidence or regulatory divergence between subspecies, cis-regulatory divergence was more common than other types. In the D. pseudoobscura subspecies pair, I compared sequence and expression divergence and found no support for directional selection driving gene misexpression in their hybrids. Allele-specific expression analysis revealed that compensatory cis-trans mutations partly explained gene misexpression in the hybrids. The remaining hybrid misexpression occurs due to interacting gene networks or possible co-option of cis-regulatory elements by divergent transacting factors. Overall, the results of this thesis highlight the role of regulatory interactions in a hybrid genome and how these interactions could lead to hybrid breakdown by disrupting gene interaction networks.

scholarly journals Allele specific expression analysis identifies regulatory variation associated with stress-related genes in the Mexican highland maize landrace Palomero Toluqueño

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3737 ◽  
Author(s):  
M. Rocío Aguilar-Rangel ◽  
Ricardo A. Chávez Montes ◽  
Eric González-Segovia ◽  
Jeffrey Ross-Ibarra ◽  
June K. Simpson ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Stress Response ◽  
Expression Analysis ◽  
Specific Expression ◽  
Allele Specific Expression ◽  
Regulatory Variation ◽  
Highland Maize ◽  
Allele Specific ◽  
Stress Related Genes ◽  
Prior Stress

BackgroundGene regulatory variation has been proposed to play an important role in the adaptation of plants to environmental stress. In the central highlands of Mexico, farmer selection has generated a unique group of maize landraces adapted to the challenges of the highland niche. In this study, gene expression in Mexican highland maize and a reference maize breeding line were compared to identify evidence of regulatory variation in stress-related genes. It was hypothesised that local adaptation in Mexican highland maize would be associated with a transcriptional signature observable even under benign conditions.MethodsAllele specific expression analysis was performed using the seedling-leaf transcriptome of an F1individual generated from the cross between the highland adapted Mexican landrace Palomero Toluqueño and the reference line B73, grown under benign conditions. Results were compared with a published dataset describing the transcriptional response of B73 seedlings to cold, heat, salt and UV treatments.ResultsA total of 2,386 genes were identified to show allele specific expression. Of these, 277 showed an expression difference between Palomero Toluqueño and B73 alleles under benign conditions that anticipated the response of B73 cold, heat, salt and/or UV treatments, and, as such, were considered to display a prior stress response. Prior stress response candidates included genes associated with plant hormone signaling and a number of transcription factors. Construction of a gene co-expression network revealed further signaling and stress-related genes to be among the potential targets of the transcription factors candidates.DiscussionPrior activation of responses may represent the best strategy when stresses are severe but predictable. Expression differences observed here between Palomero Toluqueño and B73 alleles indicate the presence ofcis-acting regulatory variation linked to stress-related genes in Palomero Toluqueño. Considered alongside gene annotation and population data, allele specific expression analysis of plants grown under benign conditions provides an attractive strategy to identify functional variation potentially linked to local adaptation.

scholarly journals SNP-specific array-based allele-specific expression analysis

2008 ◽  
Vol 18 (5) ◽  
pp. 771-779 ◽  
Author(s):  
H. T. Bjornsson ◽  
T. J. Albert ◽  
C. M. Ladd-Acosta ◽  
R. D. Green ◽  
M. A. Rongione ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Specific Expression ◽  
Allele Specific Expression ◽  
Allele Specific ◽  
Specific Array

scholarly journals Allele-specific expression analysis methods for high-density SNP microarray data

2012 ◽  
Vol 28 (8) ◽  
pp. 1102-1108 ◽  
Author(s):  
Ruijie Liu ◽  
Ana-Teresa Maia ◽  
Roslin Russell ◽  
Carlos Caldas ◽  
Bruce A. Ponder ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Microarray Data ◽  
High Density ◽  
Specific Expression ◽  
Allele Specific Expression ◽  
Snp Microarray ◽  
Analysis Methods ◽  
Allele Specific
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