Faculty Opinions recommendation of TRANSPARENT TESTA GLABRA2, a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factor.

2002 ◽  
Author(s):  
Liam Dolan
Keyword(s):  
Transcription Factor ◽  
Seed Coat ◽  
Wrky Transcription Factor ◽  
Transparent Testa

scholarly journals The transcription factor OpWRKY2 positively regulates the biosynthesis of the anticancer drug camptothecin in Ophiorrhiza pumila

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaolong Hao ◽  
Chenhong Xie ◽  
Qingyan Ruan ◽  
Xichen Zhang ◽  
Chao Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Anticancer Activity ◽  
Time Course ◽  
Indole Alkaloid ◽  
Fold Increase ◽  
Wrky Transcription Factor ◽  
Mobility Shift ◽  
Pathway Gene ◽  
Low Concentrations ◽  
Encoding Gene

AbstractThe limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry. An example of this is camptothecin, a monoterpene indole alkaloid with potent anticancer activity that is extracted at very low concentrations from woody plants. Recently, camptothecin biosynthesis has been shown to become biotechnologically amenable in hairy-root systems of the natural producer Ophiorrhiza pumila. Here, time-course expression and metabolite analyses were performed to identify novel transcriptional regulators of camptothecin biosynthesis in O. pumila. It is shown here that camptothecin production increased over cultivation time and that the expression pattern of the WRKY transcription factor encoding gene OpWRKY2 is closely correlated with camptothecin accumulation. Overexpression of OpWRKY2 led to a more than three-fold increase in camptothecin levels. Accordingly, silencing of OpWRKY2 correlated with decreased camptothecin levels in the plant. Further detailed molecular characterization by electrophoretic mobility shift, yeast one-hybrid and dual-luciferase assays showed that OpWRKY2 directly binds and activates the central camptothecin pathway gene OpTDC. Taken together, the results of this study demonstrate that OpWRKY2 acts as a direct positive regulator of camptothecin biosynthesis. As such, a feasible strategy for the over-accumulation of camptothecin in a biotechnologically amenable system is presented.

GmWRKY46, a WRKY transcription factor, negatively regulates phosphorus tolerance primarily through modifying root morphology in soybean

Plant Science ◽  
2021 ◽  
pp. 111148
Author(s):  
Xiaoqian Liu ◽  
Yuming Yang ◽  
Ruiyang Wang ◽  
Ruifan Cui ◽  
Huanqing Xu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Root Morphology ◽  
Wrky Transcription Factor

scholarly journals A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of the iso1 Promoter

2003 ◽  
Vol 15 (9) ◽  
pp. 2076-2092 ◽  
Author(s):  
Chuanxin Sun ◽  
Sara Palmqvist ◽  
Helena Olsson ◽  
Mats Borén ◽  
Staffan Ahlandsberg ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Wrky Transcription Factor ◽  
Sugar Signaling

The over-expression of a chrysanthemum WRKY transcription factor enhances aphid resistance

2015 ◽  
Vol 95 ◽  
pp. 26-34 ◽  
Author(s):  
Peiling Li ◽  
Aiping Song ◽  
Chunyan Gao ◽  
Jiafu Jiang ◽  
Sumei Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Wrky Transcription Factor ◽  
Aphid Resistance ◽  
Over Expression
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