Faculty Opinions recommendation of Identification of different binding sites in the dendritic cell-specific receptor DC-SIGN for intercellular adhesion molecule 3 and HIV-1.

2002 ◽  
Author(s):  
Siamon Gordon
Keyword(s):  
Dendritic Cell ◽  
Adhesion Molecule ◽  
Binding Sites ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Specific Receptor ◽  
Hiv 1

scholarly journals Identification of Different Binding Sites in the Dendritic Cell-specific Receptor DC-SIGN for Intercellular Adhesion Molecule 3 and HIV-1

2002 ◽  
Vol 277 (13) ◽  
pp. 11314-11320 ◽  
Author(s):  
Teunis B. H. Geijtenbeek ◽  
Gerard C. F. van Duijnhoven ◽  
Sandra J. van Vliet ◽  
Elmar Krieger ◽  
Gert Vriend ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Adhesion Molecule ◽  
Binding Sites ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Specific Receptor ◽  
Hiv 1

scholarly journals Dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin mediates HIV-1 infection of and transmission by M2a-polarized macrophages in vitro

AIDS ◽  
2013 ◽  
Vol 27 (5) ◽  
pp. 707-716 ◽  
Author(s):  
Edana Cassol ◽  
Luca Cassetta ◽  
Chiara Rizzi ◽  
Dana Gabuzda ◽  
Massimo Alfano ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Hiv 1

scholarly journals Blockade of Attachment and Fusion Receptors Inhibits HIV-1 Infection of Human Cervical Tissue

2004 ◽  
Vol 199 (8) ◽  
pp. 1065-1075 ◽  
Author(s):  
Qinxue Hu ◽  
Ines Frank ◽  
Vennansha Williams ◽  
John J. Santos ◽  
Patricia Watts ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Cervical Tissue ◽  
Lectin Receptors ◽  
Hla Dr ◽  
Mannose Binding ◽  
Migratory Cells ◽  
Hiv 1

Identification of cellular factors involved in HIV-1 entry and transmission at mucosal surfaces is critical for understanding viral pathogenesis and development of effective prevention strategies. Here we describe the evaluation of HIV-1 entry inhibitors for their ability to prevent infection of, and dissemination from, human cervical tissue ex vivo. Blockade of CD4 alone or CCR5 and CXCR4 together inhibited localized mucosal infection. However, simultaneous blockade of CD4 and mannose-binding C-type lectin receptors including dendritic cell–specific intercellular adhesion molecule–grabbing integrin was required to inhibit HIV-1 uptake and dissemination by migratory cells. In contrast, direct targeting of HIV-1 by neutralizing mAb b12 and CD4-IgG2 (PRO-542) blocked both localized infection and viral dissemination pathways. Flow cytometric analysis and immunostaining of migratory cells revealed two major populations, CD3+HLA-DR− and CD3−HLA-DR+ cells, with a significant proportion of the latter also expressing dendritic cell–specific intercellular adhesion molecule–grabbing integrin. Bead depletion studies demonstrated that such HLA-DR+ cells accounted for as much as 90% of HIV-1 dissemination. Additional studies using immature monocyte-derived dendritic cells demonstrated that although mannose-binding C-type lectin receptors and CD4 are the principal receptors for gp120, other mechanisms may account for virus capture. Our identification of the predominant receptors involved in HIV-1 infection and dissemination within human cervical tissue highlight important targets for microbicide development.

scholarly journals A Dendritic Cell–Specific Intercellular Adhesion Molecule 3–Grabbing Nonintegrin (Dc-Sign)–Related Protein Is Highly Expressed on Human Liver Sinusoidal Endothelial Cells and Promotes HIV-1 Infection

2001 ◽  
Vol 193 (6) ◽  
pp. 671-678 ◽  
Author(s):  
Arman A. Bashirova ◽  
Teunis B.H. Geijtenbeek ◽  
Gerard C.F. van Duijnhoven ◽  
Sandra J. van Vliet ◽  
Jeroen B.G. Eilering ◽  
...  
Keyword(s):  
T Cells ◽  
Endothelial Cells ◽  
Lymph Node ◽  
Dendritic Cell ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Related Molecule ◽  
In Trans ◽  
Hiv 1

The discovery of dendritic cell (DC)-specific intercellular adhesion molecule (ICAM)-3–grabbing nonintegrin (DC-SIGN) as a DC-specific ICAM-3 binding receptor that enhances HIV-1 infection of T cells in trans has indicated a potentially important role for adhesion molecules in AIDS pathogenesis. A related molecule called DC-SIGNR exhibits 77% amino acid sequence identity with DC-SIGN. The DC-SIGN and DC-SIGNR genes map within a 30-kb region on chromosome 19p13.2-3. Their strong homology and close physical location indicate a recent duplication of the original gene. Messenger RNA and protein expression patterns demonstrate that the DC-SIGN–related molecule is highly expressed on liver sinusoidal cells and in the lymph node but not on DCs, in contrast to DC-SIGN. Therefore, we suggest that a more appropriate name for the DC-SIGN–related molecule is L-SIGN, liver/lymph node–specific ICAM-3–grabbing nonintegrin. We show that in the liver, L-SIGN is expressed by sinusoidal endothelial cells. Functional studies indicate that L-SIGN behaves similarly to DC-SIGN in that it has a high affinity for ICAM-3, captures HIV-1 through gp120 binding, and enhances HIV-1 infection of T cells in trans. We propose that L-SIGN may play an important role in the interaction between liver sinusoidal endothelium and trafficking lymphocytes, as well as function in the pathogenesis of HIV-1.

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