Faculty Opinions recommendation of High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance.

2003 ◽  
Author(s):  
Andrew Bent
Keyword(s):  
Disease Resistance ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Gene Silencing ◽  
High Throughput ◽  
Plant Disease Resistance ◽  
Plant Disease ◽  
Heat Shock Protein 90 ◽  
Virus Induced Gene Silencing

scholarly journals Knock-Down of Heat-Shock Protein 90 and Isocitrate Lyase Gene Expression Reduced Root-Knot Nematode Reproduction

2015 ◽  
Vol 105 (5) ◽  
pp. 628-637 ◽  
Author(s):  
Isabela Tristan Lourenço-Tessutti ◽  
José Dijair Antonino Souza Junior ◽  
Diogo Martins-de-Sa ◽  
Antônio Américo Barbosa Viana ◽  
Regina Maria Dechechi Gomes Carneiro ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Gene Silencing ◽  
Isocitrate Lyase ◽  
Heat Shock Protein 90 ◽  
Wild Type ◽  
Gall Formation ◽  
Root Knot Nematode ◽  
Nematode Reproduction ◽  
Knock Down

Crop losses caused by nematode infections are estimated to be valued at USD 157 billion per year. Meloidogyne incognita, a root-knot nematode (RKN), is considered to be one of the most important plant pathogens due to its worldwide distribution and the austere damage it can cause to a large variety of agronomically important crops. RNA interference (RNAi), a gene silencing process, has proven to be a valuable biotechnology alternative method for RKN control. In this study, the RNAi approach was applied, using fragments of M. incognita genes that encode for two essential molecules, heat-shock protein 90 (HSP90) and isocitrate lyase (ICL). Plant-mediated RNAi of these genes led to a significant level of resistance against M. incognita in the transgenic Nicotiana tabacum plants. Bioassays of plants expressing HSP90 dsRNA demonstrated a delay in gall formation and up to 46% reduction in eggs compared with wild-type plants. A reduction in the level of HSP90 transcripts was observed in recovered eggs from plants expressing dsRNA, indicating that gene silencing persisted and was passed along to first progeny. The ICL knock-down had no clear effect on gall formation but resulted in up to 77% reduction in egg oviposition compared with wild-type plants. Our data suggest that both genes may be involved in RKN development and reproduction. Thus, in this paper, we describe essential candidate genes that could be applied to generate genetically modified crops, using the RNAi strategy to control RKN parasitism.

High-throughput screening assay for inhibitors of heat-shock protein 90 ATPase activity

2004 ◽  
Vol 327 (2) ◽  
pp. 176-183 ◽  
Author(s):  
Martin G Rowlands ◽  
Yvette M Newbatt ◽  
Chrisostomos Prodromou ◽  
Laurence H Pearl ◽  
Paul Workman ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Atpase Activity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Heat Shock Protein 90 ◽  
Screening Assay ◽  
High Throughput Screening Assay

scholarly journals Heat shock protein 90 and its co-chaperone protein phosphatase 5 interact with distinct regions of the tomato I-2 disease resistance protein

2005 ◽  
Vol 43 (2) ◽  
pp. 284-298 ◽  
Author(s):  
Sergio De La Fuente Bentem van ◽  
Jack H. Vossen ◽  
Klaas Jan Vries ◽  
Saskia Wees ◽  
Wladimir I.L. Tameling ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Phosphatase ◽  
Heat Shock Protein 90 ◽  
Protein Phosphatase 5 ◽  
Chaperone Protein ◽  
Disease Resistance Protein ◽  
Resistance Protein

Glycyrrhizin effects rat hepatocytes via the reduction of heat shock protein 90 expression

2001 ◽  
Vol 120 (5) ◽  
pp. A357-A357
Author(s):  
T YOH ◽  
T NAKASHIMA ◽  
Y SUMIDA ◽  
Y KAKISAKA ◽  
H ISHIKAWA ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 90 ◽  
Rat Hepatocytes
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