Faculty Opinions recommendation of The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos.

2001 ◽  
Author(s):  
Chaya Kalcheim
Keyword(s):  
Transcription Factor ◽  
Neural Crest ◽  
Winged Helix ◽  
Avian Embryos ◽  
Winged Helix Transcription Factor

Essential roles of the winged helix transcription factor MFH-1 in aortic arch patterning and skeletogenesis

Development ◽  
1997 ◽  
Vol 124 (22) ◽  
pp. 4627-4638 ◽  
Author(s):  
K. Iida ◽  
H. Koseki ◽  
H. Kakinuma ◽  
N. Kato ◽  
Y. Mizutani-Koseki ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Neural Crest ◽  
Aortic Arch ◽  
Interrupted Aortic Arch ◽  
Targeted Mutagenesis ◽  
Winged Helix ◽  
Cartilaginous Tissues ◽  
Early Embryos ◽  
Skeletal Defects ◽  
Winged Helix Transcription Factor

Mesenchyme Fork Head-1 (MFH-1) is a forkhead (also called winged helix) transcription factor defined by a common 100-amino acid DNA-binding domain. MFH-1 is expressed in non-notochordal mesoderm in the prospective trunk region and in cephalic neural-crest and cephalic mesoderm-derived mesenchymal cells in the prechordal region of early embryos. Subsequently, strong expression is localized in developing cartilaginous tissues, kidney and dorsal aortas. To investigate the developmental roles of MFH-1 during embryogenesis, mice lacking the MFH-1 locus were generated by targeted mutagenesis. MFH-1-deficient mice died embryonically and perinatally, and exhibited interrupted aortic arch and skeletal defects in the neurocranium and the vertebral column. Interruption of the aortic arch seen in the mutant mice was the same as in human congenital anomalies. These results suggest that MFH-1 has indispensable roles during the extensive remodeling of the aortic arch in neural-crest-derived cells and in skeletogenesis in cells derived from the neural crest and the mesoderm.

The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate

Development ◽  
2001 ◽  
Vol 128 (21) ◽  
pp. 4127-4138 ◽  
Author(s):  
Mirella Dottori ◽  
Michael K. Gross ◽  
Patricia Labosky ◽  
Martyn Goulding
Keyword(s):  
Transcription Factor ◽  
Neural Crest ◽  
Cell Fate ◽  
Neural Tube ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Cell Types ◽  
Winged Helix ◽  
Multiple Cell ◽  
Winged Helix Transcription Factor

The neural crest is a migratory cell population that gives rise to multiple cell types in the vertebrate embryo. The intrinsic determinants that segregate neural crest cells from multipotential dorsal progenitors within the neural tube are poorly defined. In this study, we show that the winged helix transcription factor Foxd3 is expressed in both premigratory and migratory neural crest cells. Foxd3 is genetically downstream of Pax3 and is not expressed in regions of Pax3 mutant mice that lack neural crest, implying that Foxd3 may regulate aspects of the neural crest differentiation program. We show that misexpression of Foxd3 in the chick neural tube promotes a neural crest-like phenotype and suppresses interneuron differentiation. Cells that ectopically express Foxd3 upregulate HNK1 and Cad7, delaminate and emigrate from the neural tube at multiple dorsoventral levels. Foxd3 does not induce Slug and RhoB, nor is its ability to promote a neural crest-like phenotype enhanced by co-expression of Slug. Together these results suggest Foxd3 can function independently of Slug and RhoB to promote the development of neural crest cells from neural tube progenitors.

scholarly journals Graded phenotypic response to partial and complete deficiency of a brain-specific transcript variant of the winged helix transcription factor RFX4

Development ◽  
2003 ◽  
Vol 130 (19) ◽  
pp. 4539-4552 ◽  
Author(s):  
Perry J. Blackshear ◽  
Joan P. Graves ◽  
Deborah J. Stumpo ◽  
Inma Cobos ◽  
John L. R. Rubenstein ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcript Variant ◽  
Winged Helix ◽  
Specific Transcript ◽  
Phenotypic Response ◽  
Winged Helix Transcription Factor

The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos

Development ◽  
2001 ◽  
Vol 128 (8) ◽  
pp. 1467-1479 ◽  
Author(s):  
R. Kos ◽  
M.V. Reedy ◽  
R.L. Johnson ◽  
C.A. Erickson
Keyword(s):  
Transcription Factors ◽  
Neural Crest ◽  
Antisense Oligonucleotides ◽  
Neural Crest Cells ◽  
Winged Helix ◽  
Dorsal Neural Tube ◽  
Neural Crest Development ◽  
Mesenchymal Transformation ◽  
Winged Helix Transcription Factor

The winged-helix or forkhead class of transcription factors has been shown to play important roles in cell specification and lineage segregation. We have cloned the chicken homolog of FoxD3, a member of the winged-helix class of transcription factors, and analyzed its expression. Based on its expression in the dorsal neural tube and in all neural crest lineages except the late-emigrating melanoblasts, we predicted that FoxD3 might be important in the segregation of the neural crest lineage from the neural epithelium, and for repressing melanogenesis in early-migrating neural crest cells. Misexpression of FoxD3 by electroporation in the lateral neural epithelium early in neural crest development produced an expansion of HNK1 immunoreactivity throughout the neural epithelium, although these cells did not undergo an epithelial/mesenchymal transformation. To test whether FoxD3 represses melanogenesis in early migrating neural crest cells, we knocked down expression in cultured neural crest with antisense oligonucleotides and in vivo by treatment with morpholino antisense oligonucleotides. Both experimental approaches resulted in an expansion of the melanoblast lineage, probably at the expense of neuronal and glial lineages. Conversely, persistent expression of FoxD3 in late-migrating neural crest cells using RCAS viruses resulted in the failure of melanoblasts to develop. We suggest that FoxD3 plays two important roles in neural crest development. First, it is involved in the segregation of the neural crest lineage from the neuroepithelium. Second, it represses melanogenesis, thereby allowing other neural crest derivatives to differentiate during the early stages of neural crest patterning.

scholarly journals The Winged Helix Transcription Factor Foxa3 Regulates Adipocyte Differentiation and Depot-Selective Fat Tissue Expansion

2013 ◽  
Vol 33 (17) ◽  
pp. 3392-3399 ◽  
Author(s):  
Lingyan Xu ◽  
Valentine Panel ◽  
Xinran Ma ◽  
Chen Du ◽  
Lynne Hugendubler ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Adipocyte Differentiation ◽  
Tissue Expansion ◽  
Fat Tissue ◽  
Winged Helix ◽  
Winged Helix Transcription Factor

scholarly journals Crystal Structure of the Virulence Gene Activator AphA fromVibrio choleraeReveals It Is a Novel Member of the Winged Helix Transcription Factor Superfamily

2005 ◽  
Vol 280 (14) ◽  
pp. 13779-13783 ◽  
Author(s):  
Rukman S. De Silva ◽  
Gabriela Kovacikova ◽  
Wei Lin ◽  
Ronald K. Taylor ◽  
Karen Skorupski ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Transcription Factor ◽  
Virulence Gene ◽  
Winged Helix ◽  
Winged Helix Transcription Factor
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