Faculty Opinions recommendation of The Partner of Inscuteable/Discs-large complex is required to establish planar polarity during asymmetric cell division in Drosophila.

2001 ◽  
Author(s):  
Jeffrey Axelrod
Keyword(s):  
Cell Division ◽  
Asymmetric Cell Division ◽  
Planar Polarity ◽  
Large Complex ◽  
Discs Large

scholarly journals The Partner of Inscuteable/Discs-Large Complex Is Required to Establish Planar Polarity during Asymmetric Cell Division in Drosophila

Cell ◽  
2001 ◽  
Vol 106 (3) ◽  
pp. 355-366 ◽  
Author(s):  
Yohanns Bellaı̈che ◽  
Anna Radovic ◽  
Daniel F. Woods ◽  
Colleen D. Hough ◽  
Marie-Laure Parmentier ◽  
...  
Keyword(s):  
Cell Division ◽  
Asymmetric Cell Division ◽  
Planar Polarity ◽  
Large Complex ◽  
Discs Large

scholarly journals The Conserved Discs-large Binding Partner Banderuola Regulates Asymmetric Cell Division in Drosophila

2014 ◽  
Vol 24 (16) ◽  
pp. 1811-1825 ◽  
Author(s):  
Federico Mauri ◽  
Ilka Reichardt ◽  
Jennifer L. Mummery-Widmer ◽  
Masakazu Yamazaki ◽  
Juergen A. Knoblich
Keyword(s):  
Cell Division ◽  
Asymmetric Cell Division ◽  
Binding Partner ◽  
Discs Large

scholarly journals Activation of Discs large by aPKC aligns the mitotic spindle to the polarity axis during asymmetric cell division

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Ognjen Golub ◽  
Brett Wee ◽  
Rhonda A Newman ◽  
Nicole M Paterson ◽  
Kenneth E Prehoda
Keyword(s):  
Cell Division ◽  
Mitotic Spindle ◽  
Asymmetric Cell Division ◽  
Intramolecular Interaction ◽  
Spindle Orientation ◽  
Orientation Factor ◽  
Daughter Cells ◽  
Kinase C ◽  
Discs Large ◽  
Fate Determinants

Asymmetric division generates cellular diversity by producing daughter cells with different fates. In animals, the mitotic spindle aligns with Par complex polarized fate determinants, ensuring that fate determinant cortical domains are bisected by the cleavage furrow. Here, we investigate the mechanisms that couple spindle orientation to polarity during asymmetric cell division of Drosophila neuroblasts. We find that the tumor suppressor Discs large (Dlg) links the Par complex component atypical Protein Kinase C (aPKC) to the essential spindle orientation factor GukHolder (GukH). Dlg is autoinhibited by an intramolecular interaction between its SH3 and GK domains, preventing Dlg interaction with GukH at cortical sites lacking aPKC. When co-localized with aPKC, Dlg is phosphorylated in its SH3 domain which disrupts autoinhibition and allows GukH recruitment by the GK domain. Our work establishes a molecular connection between the polarity and spindle orientation machineries during asymmetric cell division.

Centromere assembly and non-random sister chromatid segregation in stem cells

2020 ◽  
Vol 64 (2) ◽  
pp. 223-232 ◽  
Author(s):  
Ben L. Carty ◽  
Elaine M. Dunleavy
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Cell Fate ◽  
Sister Chromatid ◽  
Asymmetric Cell Division ◽  
Protein A ◽  
Germline Stem Cells ◽  
Sister Chromatids ◽  
Centromere Protein A ◽  
Oocyte Meiosis

Abstract Asymmetric cell division (ACD) produces daughter cells with separate distinct cell fates and is critical for the development and regulation of multicellular organisms. Epigenetic mechanisms are key players in cell fate determination. Centromeres, epigenetically specified loci defined by the presence of the histone H3-variant, centromere protein A (CENP-A), are essential for chromosome segregation at cell division. ACDs in stem cells and in oocyte meiosis have been proposed to be reliant on centromere integrity for the regulation of the non-random segregation of chromosomes. It has recently been shown that CENP-A is asymmetrically distributed between the centromeres of sister chromatids in male and female Drosophila germline stem cells (GSCs), with more CENP-A on sister chromatids to be segregated to the GSC. This imbalance in centromere strength correlates with the temporal and asymmetric assembly of the mitotic spindle and potentially orientates the cell to allow for biased sister chromatid retention in stem cells. In this essay, we discuss the recent evidence for asymmetric sister centromeres in stem cells. Thereafter, we discuss mechanistic avenues to establish this sister centromere asymmetry and how it ultimately might influence cell fate.

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