Faculty Opinions recommendation of Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer.

2001 ◽  
Author(s):  
Andrew Lumsden
Keyword(s):  
Green Fluorescent Protein ◽  
Motor Neurons ◽  
Fluorescent Protein ◽  
Transgenic Zebrafish ◽  
Islet 1 ◽  
Green Fluorescent

Laser-induced gene expression in specific cells of transgenic zebrafish

Development ◽  
2000 ◽  
Vol 127 (9) ◽  
pp. 1953-1960 ◽  
Author(s):  
M.C. Halloran ◽  
M. Sato-Maeda ◽  
J.T. Warren ◽  
F. Su ◽  
Z. Lele ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent Protein Gene ◽  
Transgenic Zebrafish ◽  
Hsp70 Promoter ◽  
Expression Of Genes ◽  
Transgenic Lines ◽  
Transgenic Embryos ◽  
Green Fluorescent

Over the past few years, a number of studies have described the generation of transgenic lines of zebrafish in which expression of reporters was driven by a variety of promoters. These lines opened up the real possibility that transgenics could be used to complement the genetic analysis of zebrafish development. Transgenic lines in which the expression of genes can be regulated both in space and time would be especially useful. Therefore, we have cloned the zebrafish promoter for the inducible hsp70 gene and made stable transgenic lines of zebrafish that express the reporter green fluorescent protein gene under the control of a hsp70 promoter. At normal temperatures, green fluorescent protein is not detectable in transgenic embryos with the exception of the lens, but is robustly expressed throughout the embryo following an increase in ambient temperature. Furthermore, we have taken advantage of the accessibility and optical clarity of the embryos to express green fluorescent protein in individual cells by focussing a sublethal laser microbeam onto them. The targeted cells appear to develop normally: cells migrate normally, neurons project axons that follow normal pathways, and progenitor cells divide and give rise to normal progeny cells. By generating other transgenic lines in which the hsp70 promoter regulates genes of interest, it should be possible to examine the in vivo activity of the gene products by laser-inducing specific cells to express them in zebrafish embryos. As a first test, we laser-induced single muscle cells to make zebrafish Sema3A1, a semaphorin that is repulsive for specific growth cones, in a hsp70-sema3A1 transgenic line of zebrafish and found that extension by the motor axons was retarded by the induced muscle.

scholarly journals Development of multilineage adult hematopoiesis in the zebrafish with a runx1 truncation mutation

Blood ◽  
2010 ◽  
Vol 115 (14) ◽  
pp. 2806-2809 ◽  
Author(s):  
Raman Sood ◽  
Milton A. English ◽  
Christiane L. Belele ◽  
Hao Jin ◽  
Kevin Bishop ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Hematopoietic Stem Cells ◽  
Fluorescent Protein ◽  
Lineage Tracing ◽  
Transgenic Zebrafish ◽  
Hematopoietic Stem ◽  
Truncation Mutation ◽  
Green Fluorescent ◽  
Definitive Hematopoiesis

Abstract Runx1 is required for the emergence of hematopoietic stem cells (HSCs) from hemogenic endothelium during embryogenesis. However, its role in the generation and maintenance of HSCs during adult hematopoiesis remains uncertain. Here, we present analysis of a zebrafish mutant line carrying a truncation mutation, W84X, in runx1. The runx1W84X/W84X embryos showed blockage in the initiation of definitive hematopoiesis, but some embryos were able to recover from a larval “bloodless” phase and develop to fertile adults with multilineage hematopoiesis. Using cd41–green fluorescent protein transgenic zebrafish and lineage tracing, we demonstrated that the runx1W84X/W84X embryos developed cd41+ HSCs in the aorta-gonad-mesonephros region, which later migrated to the kidney, the site of adult hematopoiesis. Overall, our data suggest that in zebrafish adult HSCs can be formed without an intact runx1.

Requirements for green fluorescent protein detection in transgenic zebrafish embryos

Gene ◽  
1996 ◽  
Vol 173 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Adam Amsterdam ◽  
Shuo Lin ◽  
Larry G. Moss ◽  
Nancy Hopkins
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Protein Detection ◽  
Transgenic Zebrafish ◽  
Zebrafish Embryos ◽  
Green Fluorescent

Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon

2011 ◽  
Vol 240 (11) ◽  
pp. 2539-2547 ◽  
Author(s):  
Yanwei Xi ◽  
Man Yu ◽  
Rafael Godoy ◽  
Gary Hatch ◽  
Luc Poitras ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Dopaminergic Neurons ◽  
Fluorescent Protein ◽  
Transgenic Zebrafish ◽  
Green Fluorescent ◽  
Ventral Diencephalon
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