scholarly journals New Records of Dactylobiotus parthenogeneticus Bertolani, 1982 Provide Insight into Its Genetic Variability and Geographic Distribution

2020 ◽  
Vol 68 (2) ◽  
pp. 57-72 ◽  
Author(s):  
Justyna Pogwizd ◽  
Daniel Stec
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Haplotype Diversity ◽  
Original Description ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Morphological Identification ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
Insight Into

In sediment samples collected from three distinct European locations (United Kingdom, France, Poland), populations of Dactylobiotus parthenogeneticus were found. The original description of this species was based solely on the morphology observed with light microscopy and later supplemented by some additional SEM data of the buccal apparatus and DNA sequences of 18S rRNA and COI. Here we provide an updated description of the species by means of integrative taxonomy. The description comprises a comprehensive set of morphometric and morphological data from light and scanning microscopy as well as nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) fragments. Our analysis of haplotype diversity confirmed our morphological identification and showed that D. parthenogeneticus is widely distributed in Europe.

Phylogenetic position of two Macrobiotus species with a revisional note on Macrobiotus sottilei Pilato, Kiosya, Lisi & Sabella, 2012 (Tardigrada: Eutardigrada: Macrobiotidae)

Zootaxa ◽  
2021 ◽  
Vol 4933 (1) ◽  
pp. 113-135
Author(s):  
YEVGEN KIOSYA ◽  
JUSTYNA POGWIZD ◽  
YELYZAVETA MATSKO ◽  
MATTEO VECCHI ◽  
DANIEL STEC
Keyword(s):  
Dna Sequences ◽  
Original Description ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
The Family ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

In a moss and lichen sample collected on the Polish coast, a new population of Macrobiotus sottilei was found. Given that the original description of M. sottilei was based solely on the morphology observed under light microscopy and measurements of a few individuals, we provide, by means of integrative taxonomy, a revisional note on this species. We present a comprehensive set of morphometric and morphological data from light and scanning electron microscopy analysis together with nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragments. We also provide the same set of DNA sequences for Macrobiotus glebkai from a population recently found in Eastern Ukraine and elucidate the phylogenetic position of these two taxa within the family Macrobiotidae. Moreover, the terminology of cuticular bars in macrobiotid legs, and pillars within the egg chorion ornamentation in the Macrobiotus hufelandi morphogroup, are also discussed. 

scholarly journals Integrative descriptions of two new Macrobiotus species (Tardigrada, Eutardigrada, Macrobiotidae) from Mississippi (USA) and Crete (Greece)

2021 ◽  
Vol 97 (1) ◽  
pp. 281-306
Author(s):  
Matteo Vecchi ◽  
Daniel Stec
Keyword(s):  
18S Rrna ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Distal Portion ◽  
Morphological Data ◽  
28S Rrna ◽  
Morphometric Characters ◽  
Clade B ◽  
Specific Morphology ◽  
Definition Of

In this paper, we describe two new Macrobiotus species from Mississippi (USA) and Crete (Greece) by means of integrative taxonomy. Detailed morphological data from light and scanning electron microscopy, as well as molecular data (sequences of four genetic markers: 18S rRNA, 28S rRNA, ITS-2 and COI), are provided in support of the descriptions of the new species. Macrobiotus annewintersaesp. nov. from Mississippi belongs to the Macrobiotus persimilis complex (Macrobiotus clade B) and exhibits a unique egg processes morphology, similar only to Macrobiotus anemone Meyer, Domingue & Hinton, 2014, but mainly differs from that species by the presence of eyes, granulation on all legs, dentate lunulae on legs IV, and of bubble-like structures within the tentacular arms that are present on the distal portion of the egg processes. Macrobiotus rybakisp. nov. from Crete belongs to the Macrobiotus clade A and is most similar to Macrobiotus dariae Pilato & Bertolani, 2004, Macrobiotus noemiae Roszkowska & Kaczmarek, 2019, Macrobiotus santoroi Pilato & D’Urso, 1976, and Macrobiotus serratus Bertolani, Guidi & Rebecchi, 1996, but differs from them mainly in the morphological details of its egg processes and chorion reticulation, but also by a number of morphometric characters. In light of the specific morphology of the egg processes of Macrobiotus annewintersaesp. nov. and Macrobiotus anemone, that are equipped with tentacular arms instead of proper terminal disc, we also provide an updated definition of the Macrobiotus persimilis complex.

scholarly journals Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

2020 ◽  
Author(s):  
Daniel Stec ◽  
Denis T. Tumanov ◽  
Reinhardt Møbjerg Kristensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
Morphometric Analyses ◽  
Taxonomic Methods ◽  
Two Populations ◽  
Scanning Electron

In this paper we describe Macrobiotus engbergi sp. nov. and Tenuibiotus zandrae sp. nov. from Greenland. Our study has involved both classical taxonomic methods, which include morphological and morphometric analyses conducted with the use of light and scanning electron microscopy, and genetic analysis based on four molecular markers (three nuclear: 18S rRNA, 28S rRNA, ITS-2, and one mitochondrial: COI). Moreover, we re-examined the type series of Tenuibiotus voronkovi (Tumanov, 2007) as well as the original sample where the species was found and we provide new morphological data from light and scanning electron microscopy which enabled us to amend its description. Finally, we also analysed slides with animals and egg of two populations from Nordaustlandet and Edgeøya (archipelago of Svalbard, Norway) designated as T. voronkovi within its recent redescription. The results and comparisons presented in our study question the validity of this designation. 

scholarly journals Two new species of the Macrobiotus hufelandi complex (Tardigrada: Eutardigrada: Macrobiotidae) from Australia and India, with notes on their phylogenetic position

2019 ◽  
Author(s):  
Kyle Coughlan ◽  
Daniel Stec
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Taxonomic Status ◽  
Phylogenetic Position ◽  
28S Rrna ◽  
Future Studies ◽  
Mitochondrial Coi ◽  
Definition Of ◽  
Group Species ◽  
Independent Species

In this paper we describe two new tardigrade species belonging to the Macrobiotus hufelandi complex: Macrobiotus noongaris sp. nov. from Perth, Australia, and Macrobiotus kamilae sp. nov. from Mussoorie, India. Live specimens extracted from moss samples were used to establish laboratory cultures in order to obtain additional animals and eggs needed for their integrative descriptions. These descriptions are based on traditional morphological and morphometric data collected using both light and scanning electron microscopy, which, at the same time, were associated with DNA sequences of four genetic markers, three nuclear (18S rRNA, 28S rRNA and ITS-2) and one mitochondrial (COI). The use of DNA sequences allowed for a more accurate verification of the taxonomic status of M. noongaris sp. nov. and M. kamilae sp. nov as independent species of the hufelandi group. Although they both exhibit typical inverted goblet-shaped processes, they represent a recently discovered clade, which was thought to group species with modified morphology of egg processes. Thus, this contribution expands the definition of the mentioned clade and constitutes another link that will be helpful for future studies on the evolution of the M. hufelandi complex.

An integrative description of Ramazzottius subanomalus (Biserov, 1985 (Tardigrada) from Poland

Zootaxa ◽  
2017 ◽  
Vol 4300 (3) ◽  
pp. 403 ◽  
Author(s):  
DANIEL STEC ◽  
KRZYSZTOF ZAWIERUCHA ◽  
ŁUKASZ MICHALCZYK
Keyword(s):  
Dna Sequences ◽  
Original Description ◽  
Molecular Data ◽  
28S Rrna ◽  
Morphometric Traits ◽  
Concrete Wall ◽  
Morphometric Characters ◽  
Mitochondrial Coi ◽  
Qualitative And Quantitative ◽  
Quantitative Characters

A population of Ramazzottius subanomalus (Biserov, 1985) was found in a moss sample collected from concrete wall in Poznań, western Poland. Animals were prepared for light and scanning electron microscopy and for DNA sequencing to provide an integrative description of the species that was originally described only by means of classical alpha taxonomy. As a result of our studies, we provide the first ever SEM photomicrographs of Ramazzottius subanomalus individuals and their buccal apparatuses. Additionally, we present new DNA sequences as well as new morphometric data for R. subanomalus. The molecular data comprise sequences for three DNA fragments, one mitochondrial (COI) and two nuclear (18S rRNA and 28S rRNA). As a result of being able to analyse a considerable number of animals and eggs, our study has expanded some of R. subanomalus morphometric traits ranges; compared with those provided in the original description. The spine-shaped egg processes  as well as qualitative and quantitative characters of adults show R. subanomalus is most similar to Ramazzottius anomalus (Ramazzotti, 1962). However, our study has shown that R. subanomalus differs from R. anomalus by the lack of fine granulation on eggshell surface as well as by three other morphometric characters: longer buccal tube and two aspects of the placoids. We also discuss the validity of the R. anomalus record in Poland in the light of our findings. Finally, we show that the extreme eggshell variability in this species is observed both in natural and laboratory environments. 

An integrative description of Diploechiniscus oihonnae (Richters, 1903) population from near the original type locality in Merok (Norway)

Zootaxa ◽  
2021 ◽  
Vol 4964 (1) ◽  
pp. 83-102
Author(s):  
ŁUKASZ KACZMAREK ◽  
PUSHPALATA KAYASTHA ◽  
MAGDALENA GAWLAK ◽  
MONIKA MIODUCHOWSKA ◽  
MILENA ROSZKOWSKA
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Morphological Variability ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Genetic Differences ◽  
Type Locality ◽  
28S Rrna ◽  
Life Stages ◽  
Original Type

In the present study, we used integrative taxonomy to describe a population of Diploechiniscus oihonnae (Richters, 1903) from the neotype locality in Merok (Norway). We found no differences in the chaetotaxy formula between the life stages and sex of Dpl. oihonnae. The presence of filaments at Bd in some specimens of Dpl. oihonnae appears to be random and most likely represents the morphological variability of this species. We also obtained DNA sequences of 18S rRNA, 28S rRNA, ITS-2, and COI of Dpl. oihonnae from the neotype locality for comparison with the sequences available in GenBank, which showed low genetic differences between the neotypic population and specimens from other localities. Therefore, we decided to establish our specimens from Merok as neotype and neoparatypes of Dpl. oihonnae. Additionally, based on morphological characters, Dpl. horningi (Schuster & Grigarick, 1971) was synonymised with Dpl. oihonnae.

An integrative description of Pilatobius nuominensis sp. nov. (Tardigrada: Hypsibiidae) from China

Zootaxa ◽  
2021 ◽  
Vol 5026 (1) ◽  
pp. 65-76
Author(s):  
XUE-LING SUN ◽  
JING-YU ZHANG ◽  
NING WANG ◽  
MIN ZHAO ◽  
XUE-GANG LUO
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Taxonomic Status ◽  
First Record ◽  
Nucleotide Sequences ◽  
28S Rrna ◽  
Dna Fragments ◽  
Caudal Region ◽  
Mitochondrial Coi ◽  
Independent Verification

A newly identified tardigrade species from China, Pilatobius nuominensis sp. nov., belongs to the group of species with cuticle of the dorsal and lateral caudal region with evident irregular polygonal sculpture. Nucleotide sequences of two nuclear (18S rRNA, 28S rRNA) and one mitochondrial (COI) DNA fragments of the new species are provided, which allows an independent verification of the taxonomic status of the new species. This is the first record of the genus Pilatobius in the Great Hinggan Mountains.  

scholarly journals Comparative morphometrics and ribosomal DNA sequence analysis of Longidorus orientalis Loof, 1983 (Nematoda: Longidoridae) from Spain and Iran

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 631-640 ◽  
Author(s):  
Juan E. Palomares-Rius ◽  
Blanca B. Landa ◽  
Zahra Tanha Maafi ◽  
David J. Hunt ◽  
Pablo Castillo
Keyword(s):  
18S Rrna ◽  
Date Palm ◽  
Original Description ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Khuzestan Province ◽  
South West ◽  
Graminaceous Plants ◽  
Partial 18S ◽  
Ribosomal Dna Sequence

Abstract During recent nematode surveys in a muddy soil around undetermined graminaceous plants in El Rocío, Huelva Province, in southern Spain, and from the rhizosphere of date palm associated with graminaceous vegetation from Abadan, in Khuzestan Province, south-west Iran, populations of Longidorus orientalis were identified. Morphological and morphometrical studies on these populations fit the original description and represent the first report from Spain and Europe. Molecular characterisation of L. orientalis from Spain and Iran, using D2-D3 expansion regions of 28S rRNA, 18S rRNA and ITS-rRNA, is provided. Sequences of the D2-D3 expansion regions and partial 18S genes were analysed using maximum likelihood and Bayesian inference to reconstruct phylogenetic relationships within L. orientalis and other Longidorus species. The results revealed a closer phylogenetic relationship with L. goodeyi for the D2-D3 expansion region and with L. vineacola for the partial 18S region.

Phylogenetic relationships and classification of the Malagasy pseudoxyrhophiine snake genera Geodipsas and Compsophis based on morphological and molecular data

Zootaxa ◽  
2007 ◽  
Vol 1517 (1) ◽  
pp. 53-62 ◽  
Author(s):  
FRANK GLAW ◽  
ZOLTÁN T. NAGY ◽  
MIGUEL VENCES
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Close Relationships ◽  
Original Description ◽  
Molecular Data ◽  
Morphological Data ◽  
Molecular Phylogenetic ◽  
Species Groups ◽  
Morphological And Molecular Data

Based on a specimen found at Montagne d'Ambre in northern Madagascar morphologically agreeing with Compsophis albiventris Mocquard, 1894, we report on the rediscovery of this enigmatic snake genus and species and its molecular phylogenetic relationships. Compsophis albiventris, considered to be the only representative of its genus and unreported since its original description, bears strong morphological similarities to species of Geodipsas Boulenger, 1896. A molecular phylogeny based on DNA sequences of three mitochondrial and nuclear genes (complete cytochrome b, fragments of 16S rRNA and c-mos) in Compsophis albiventris and three Geodipsas species corroborated close relationships between C. albiventris and Geodipsas boulengeri, and showed that the genera Compsophis and Geodipsas together form a monophyletic unit. Despite the general similarities, morphological data and chromatic features support the existence of two species groups, corresponding to Compsophis and Geodipsas. We consequently consider Geodipsas as a subgenus of Compsophis and transfer all species currently in Geodipsas into the genus Compsophis.

scholarly journals Integrative Descriptions of Two New Tardigrade Species along with the New Record of Mesobiotus skorackii Kaczmarek et al., 2018 from Canada

Diversity ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 394
Author(s):  
Pushpalata Kayastha ◽  
Milena Roszkowska ◽  
Monika Mioduchowska ◽  
Magdalena Gawlak ◽  
Łukasz Kaczmarek
Keyword(s):  
Large Population ◽  
Original Description ◽  
Molecular Data ◽  
28S Rrna ◽  
Moss Sample ◽  
Morphometric Characters ◽  
Mitochondrial Coi ◽  
Canadian Population ◽  
Coi Sequences ◽  
Morphology And Morphometry

Two new tardigrade species from a moss sample collected in Canada, one representing Macrobiotus hufelandi complex and the second one belonging to the genus Bryodelphax, are described. Integrative analysis was undertaken based on morphological and morphometric data (using both light and scanning electron microscopy (SEM)) combined with multilocus molecular analysis (nuclear sequences, i.e., 18S rRNA, 28S rRNA and ITS-2 as well as mitochondrial COI barcode sequences). Based on COI sequences, Macrobiotus birendrai sp. nov. is most similar to Mac. canaricus (p-distance 17%), whereas Bryodelphax mareki sp. nov. is most similar to Bry. parvulus (p-distance 16%). Both species differ also from their congeners in some morphological and morphometric characters of adults and/or details of egg chorion. Additionally, a large population of Mesobiotus skorackii was found in the sample and this is the first report of this species outside its terra typica in Kirghizia. The original description of this species was prepared based solely on the morphology and morphometry, therefore, here we provide updated data for this species enclosing morphometric and molecular data for the Canadian population.

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