The cAMP Analogue, dbcAMP, Affects Rabbit Ovarian Cell Proliferation, Apoptosis, Release of Steroids and Response to Hormones

2014 ◽  
Vol 62 (3) ◽  
pp. 211-218 ◽  
Author(s):  
Alexander V. Sirotkin ◽  
Andrej Balazi ◽  
Peter Chrenek
Keyword(s):  
Cell Proliferation ◽  
Ovarian Cell ◽  
Camp Analogue

cAMP response element-binding protein 1 controls porcine ovarian cell proliferation, apoptosis, and FSH and insulin-like growth factor 1 response

2018 ◽  
Vol 30 (8) ◽  
pp. 1145 ◽  
Author(s):  
A. V. Sirotkin ◽  
A. Benčo ◽  
A. Tandlmajerová ◽  
M. Lauková ◽  
D. Vašíček ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Camp Response Element Binding ◽  
Insulin Like Growth Factor ◽  
Camp Response Element ◽  
Response Element ◽  
Ovarian Cell ◽  
Proliferation And Apoptosis ◽  
Element Binding Protein ◽  
Series Of Experiments

The aim of the present study was to examine the role of cAMP response element-binding protein (CREB) and its phosphorylation in the regulation of ovarian cell proliferation and apoptosis, and of the response of proliferation and apoptosis to the upstream hormonal stimulators FSH and insulin-like growth factor (IGF) 1. In the first series of experiments, porcine ovarian granulosa cells, transfected or not with a gene construct encoding wild-type CREB1 (CREB1WT), were cultured with and without FSH (0, 1, 10 or 100 ng mL−1). In the second series of experiments, these cells were transfected or not with CREB1WT or non-phosphorylatable mutant CREB1 (CREB1M1) and cultured with and without FSH (0, 1, 10 or 100 ng mL−1) or IGF1 (0, 1, 10 and 100 ng mL−1). Levels of total and phosphorylated (p-) CREB1, proliferating cell nuclear antigen (PCNA), a marker of proliferation, and BAX, a marker of apoptosis, were evaluated by western immunoblotting and immunocytochemical analysis. Transfection of cells with CREB1WT promoted accumulation of total CREB1 within cells, but p-CREB1 was not detected in any cell group. Both CREB1WT and CREB1M1 reduced cell proliferation and apoptosis. Addition of 10 and 100 ng mL−1 FSH to non-transfected cells promoted CREB1 accumulation and apoptosis, whereas cell proliferation was promoted by all concentrations of FSH tested. FSH activity was not modified in cells transfected with either CREB1WT or CREB1M1. IGF1 at 100 ng mL−1 promoted cell proliferation, whereas all concentrations of IGF1 tested reduced apoptosis. Transfection with either CREB1WT or CREB1M1 did not modify the effects of either FSH or IGF1, although CREB1M1 reversed the effect of IGF1 on apoptosis from inhibitory to stimulatory. These observations suggest that CREB1 is involved in the downregulation of porcine ovarian cell proliferation and apoptosis. The absence of visible CREB1 phosphorylation and the similarity between the effects of CREB1WT and CREB1M1 transfection indicate that phosphorylation is not necessary for CREB1 action on these processes. Furthermore, the observations suggest that FSH promotes both ovarian cell proliferation and apoptosis, whereas IGF1 has proliferation-promoting and antiapoptotic properties. The effect of FSH on CREB1 accumulation and the ability of CREB1M1 to reverse the effects of IGF1 on apoptosis indicate that CREB1 is a mediator of hormonal activity, but the inability of either CREB1WT or CREBM1transfection to modify the primary effects of FSH and IGF1 suggest that CREB1 and its phosphorylation do not mediate the action of these hormones on ovarian cell proliferation and apoptosis.

Developmental Programming: Impact of Prenatal Testosterone Excess on Ovarian Cell Proliferation and Survival Factors.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 641-641 ◽  
Author(s):  
Vasantha Padmanabhan ◽  
Natalia R. Salvetti ◽  
Almudena Veiga-Lopez ◽  
Hugo H. Ortega
Keyword(s):  
Cell Proliferation ◽  
Developmental Programming ◽  
Ovarian Cell ◽  
Survival Factors ◽  
Prenatal Testosterone

Neuropeptide Y directly affects ovarian cell proliferation and apoptosis

2015 ◽  
Vol 15 (4) ◽  
pp. 257-260 ◽  
Author(s):  
Alexander V. Sirotkin ◽  
Diana Kardošová ◽  
Saleh Hamad Alwasel ◽  
Abdel Halim Harrath
Keyword(s):  
Cell Proliferation ◽  
Neuropeptide Y ◽  
Ovarian Cell ◽  
Proliferation And Apoptosis

Roles of different n-3/n-6 PUFA ratios in ovarian cell development and steroidogenesis in PCOS rats

2019 ◽  
Vol 10 (11) ◽  
pp. 7397-7406 ◽  
Author(s):  
Xiaoshu Ma ◽  
Xuechun Weng ◽  
Xusong Hu ◽  
Qiaozhi Wang ◽  
Ye Tian ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Polycystic Ovary Syndrome ◽  
Granulosa Cell ◽  
Polycystic Ovary ◽  
Growth Arrest ◽  
Cell Development ◽  
Follicle Growth ◽  
Ovary Syndrome ◽  
Ovarian Cell ◽  
Endocrine Disorder

Polycystic ovary syndrome (PCOS) is a complex and common endocrine disorder characterized by hyperandrogenism, which is accompanied by follicle growth arrest at the small antral stage, minimal granulosa cell proliferation, and chronic anovulation.

Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation

Aquaculture ◽  
2016 ◽  
Vol 450 ◽  
pp. 116-122 ◽  
Author(s):  
Parinyaporn Nuurai ◽  
Scott F. Cummins ◽  
Natasha A. Botwright ◽  
Prasert Sobhon
Keyword(s):  
Cell Proliferation ◽  
Gonadotropin Releasing Hormone ◽  
Ovarian Cell ◽  
Releasing Hormone

scholarly journals Resistin is a survival factor for porcine ovarian follicular cells

Reproduction ◽  
2015 ◽  
Vol 150 (4) ◽  
pp. 343-355 ◽  
Author(s):  
Agnieszka Rak ◽  
Eliza Drwal ◽  
Anna Wróbel ◽  
Ewa Łucja Gregoraszczuk
Keyword(s):  
Cell Proliferation ◽  
Protein Expression ◽  
Western Blot ◽  
Dna Fragmentation ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Inhibitory Effect ◽  
Ovarian Cell ◽  
Proliferation And Apoptosis ◽  
Mrna And Protein Expression

Previously, we demonstrated the expression of resistin in the porcine ovary, the regulation of its expression and its direct effect on ovarian steroidogenesis. The objective of this study was to examine the effect of resistin on cell proliferation and apoptosis in a co-culture model of porcine granulosa and theca cells. First, we analysed the effect of resistin at 1 and 10 ng/ml alone or in combination with FSH- and IGF1 on ovarian cell proliferation with an alamarBlue assay and protein expression of cyclins A and B using western blot. Next, the mRNA and protein expression of selected pro-apoptotic and pro-survival regulators of cell apoptosis, caspase-9, -8 and -3 activity and DNA fragmentation using real time PCR, western blot, fluorescent assay and an ELISA kit, respectively, were analysed after resistin treatment. Furthermore, we determined the effect of resistin on the protein expression of ERK1/2, Stat and Akt kinase. Using specific inhibitors of these kinases, we also checked caspase-3 activity and protein expression. We found that resistin, at both doses, has no effect on cell proliferation. The results showed that resistin decreased pro-apoptotic genes, which was confirmed on protein expression of selected factors. We demonstrate an inhibitory effect of resistin on caspase activity and DNA fragmentation. Finally, resistin stimulated phosphorylation of the ERK1/2, Stat and Akt and kinases inhibitors reversed resistin action on caspase-3 activity and protein expression to control. All of these results showed that resistin has an inhibitory effect on porcine ovarian cell apoptosis by activation of the MAPK/ERK, JAK/Stat and Akt/PI3 kinase signalling pathways.

scholarly journals Direct inhibitory effect of flaxseed on porcine ovarian granulosa cell functions

2019 ◽  
Vol 44 (5) ◽  
pp. 507-511
Author(s):  
Aneta Štochmal’ová ◽  
Abdel Halim Harrath ◽  
Saleh Alwasel ◽  
Alexander V. Sirotkin
Keyword(s):  
Cell Proliferation ◽  
Granulosa Cells ◽  
Inhibitory Effect ◽  
Ovarian Cell ◽  
Cell Functions ◽  
Ovarian Granulosa Cell ◽  
Igf I ◽  
Direct Inhibitory Effect ◽  
Reproductive Processes ◽  
Flaxseed Extract

Flaxseed is useful as a functional food and alternative medicine owing to its beneficial health effects. Its action on ovarian cell functions and interrelationships with the upstream hormonal regulators remain unknown. Our aim was to examine the direct influence of flaxseed extract on basal porcine ovarian functions (proliferation, apoptosis), leptin release, and response to insulin-like growth factor I (IGF-I). First, we examined the effect of flaxseed extract on the accumulation of proliferation (PCNA) and apoptosis (Bax) markers and on leptin release in cultured porcine ovarian granulosa cells. Next, granulosa cells were cultured with IGF-I with and without flaxseed extract and analyzed for PCNA and Bax accumulation by quantitative immunocytochemistry and for leptin release by radioimmunoassay. Flaxseed decreased the accumulation of PCNA and increased that of Bax at all doses and reduced leptin output at 100 μg/mL. In contrast, IGF-I promoted PCNA accumulation and suppressed Bax. Flaxseed did not modify IGF-I action on these parameters. Thus, we showed that flaxseed influences porcine reproductive processes, having a direct effect on the ovary and the ability to affect ovarian cell proliferation, apoptosis, and leptin release. Furthermore, we confirmed the pro-proliferative and antiapoptotic actions of IGF-I but showed that flaxseed action on ovarian cell proliferation and apoptosis is not due to changes in the cell response to IGF-I. The potential direct anti-reproductive action of flaxseed needs to be considered during its application in nutrition, medicine, and animal production.

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