The Effect of Mammary Gland-Specific Transgene Expression on Rabbit Reproductive Gland Structure

2014 ◽  
Vol 62 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Eva Tvrdá ◽  
Peter Massányi ◽  
Norbert Lukáč ◽  
Ján Danko ◽  
Janka Schlarmannová ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Transgene Expression ◽  
Reproductive Gland

scholarly journals Effects of BRCA1 Transgene Expression on Murine Mammary Gland Development and Mutagen-Induced Mammary Neoplasia

2007 ◽  
pp. 281-291 ◽  
Author(s):  
Arichika Hoshino ◽  
Cindy J. Yee ◽  
Mel Campbell ◽  
Randall L. Woltjer ◽  
Rebecca L. Townsend ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Transgene Expression ◽  
Mammary Gland Development ◽  
Gland Development ◽  
Mammary Neoplasia

scholarly journals Nuclear factor I and mammary gland factor (STAT5) play a critical role in regulating rat whey acidic protein gene expression in transgenic mice.

1995 ◽  
Vol 15 (4) ◽  
pp. 2063-2070 ◽  
Author(s):  
S Li ◽  
J M Rosen
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Transgenic Mice ◽  
Binding Sites ◽  
Transgene Expression ◽  
Protein Gene ◽  
Whey Acidic Protein ◽  
Nuclear Factor ◽  
Factor I ◽  
Nuclear Factor I

The rat whey acidic protein (WAP) gene contains a mammary gland-specific and hormonally regulated DNase I-hypersensitive site 830 to 720 bp 5' to the site of transcription initiation. We have reported previously that nuclear factor I (NFI) binding at a palindromic site and binding at a half-site are the major DNA-protein interactions detected within this tissue-specific nuclease-hypersensitive region. We now show that point mutations introduced into these NFI-binding sites dramatically affect WAP gene expression in transgenic mice. Transgene expression was totally abrogated when the palindromic NFI site or both binding sites were mutated, suggesting that NFI is a key regulator of WAP gene expression. In addition, a recognition site for mammary gland factor (STAT5), which mediates prolactin induction of milk protein gene expression, was also identified immediately proximal to the NFI-binding sites. Mutation of this site reduced transgene expression by approximately 90% per gene copy, but did not alter tissue specificity. These results suggest that regulation of WAP gene expression is determined by the cooperative interactions among several enhancers that constitute a composite response element.

Enhancing the efficiency of transgene expression

1993 ◽  
Vol 339 (1288) ◽  
pp. 225-232 ◽  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Dna Sequences ◽  
Transgene Expression ◽  
Transgenic Animals ◽  
Genomic Segment ◽  
Protein Encoding

Two strategies for enhancing gene expression in transgenic animals are described with particular reference to targeting expression to the mammary gland. Gene constructs in which the protein-encoding DNA sequences are contained within a genomic segment (comprising most or all of the natural introns of the corresponding gene) are shown to be expressed more efficiently than their intronless counterparts. Secondly, co-integrating an otherwise poorly expressed transgene in the vicinity of an actively expressed transgene can dramatically improve its efficiency of expression.

scholarly journals Modification of Milk Composition via Transgenesis: The Role of the Extracellular Matrix in Regulating Transgene Expression

1995 ◽  
Author(s):  
Itamar Barash ◽  
J. Mina Bissell ◽  
Alexander Faerman ◽  
Moshe Shani
Keyword(s):  
Protein Synthesis ◽  
Mammary Gland ◽  
Translational Control ◽  
Transgene Expression ◽  
Milk Protein ◽  
Milk Composition ◽  
Regulatory Sequences ◽  
Enhancer Element

Altering milk composition via transgenesis depends on three main factors. (1) The availability of an efficient regulatory sequences for targeting transgene(s) to the mammary gland; (2) a reliable in vitro model to test the expression of transgenes prior to their introduction to the animal genome; and (3) better understanding of the major factors which determine the rate of gene expression and protein synthesis. The current studies provide the necessary means and knowledge to alter milk protein composition via transgenesis. The following specific goals were achieved: a: Identifying regulatory regions in the b-lactoglobulin (BLG) gene and the cross-talk between elements which enabled us to construct an efficient vector for the expression of desirable cDNA's in the mammary gland. b: The establishment of a sheep mammary cell line that serves as a model for the analysis of endogenous and exogenous milk protein synthesis in the mammary gland of livestock. c: An accurate comparison of the potency of the 5' regulatory sequences from the BLG and whey acidic protein (WAP) promoters in directing the expression of human serum albumin (HSA) to the mammary gland in vitro and in vivo. In this study we have also shown that sequences within the coding region may determine a specific pattern of expression for the transgene, distinct from that of the native milk protein genes. d: Characterizing the dominant role of ECM in transgene expression in mammary epithelial cells. e: Further characterization of the BCE-1 enhancer element in the promoter of the b-casein gene as a binding site for the c/EBP-b and Stat5. Identifying its interaction with chromatin and its up regulation by inhibitors of histone deacetylation. f: Identifying a mechanism of translational control as a mediator for the synergistic effect of insulin and prolactin on protein synthesis in the mammary gland.

scholarly journals Mammary gland specific hEGF receptor transgene expression induces neoplasia and inhibits differentiation

Oncogene ◽  
2000 ◽  
Vol 19 (17) ◽  
pp. 2129-2137 ◽  
Author(s):  
Ralf Brandt ◽  
Ralf Eisenbrandt ◽  
Frauke Leenders ◽  
Wolfgang Zschiesche ◽  
Bert Binas ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Transgene Expression
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