ANTIBIOTICS RESISTANCE AND OUTCOMES OF SEPSIS IN HUE CENTRAL HOSPITAL FROM 2009 TO 2010

2011 ◽  
pp. 53-57
Author(s):  
Thi Phuong Thao Nguyen ◽  
Xuan Chuong Tran
Keyword(s):  
Antibiotic Resistance ◽  
Retrospective Study ◽  
High Mortality ◽  
Streptococcus Suis ◽  
Antibiotics Resistance ◽  
Central Hospital ◽  
E Coli ◽  
Study Results ◽  
The Right ◽  
Very High

Objective: Sepsis is a very important infection. Studying the antibiotic resistance helps doctors to make the right choice for treatment of infections and reduce mortality. Materials and methods: 60 patients diagnosed as sepsis treated at Hue Central hospital, since Jan 2009 to June 2010. Retrospective study. Results: The most isolated agents are E. coli (40% patients), K. pneumoniae (16.7%) and Streptococcus suis (18.3%). Most of agents sensitive to ceftriaxon, ciprofloxacin và gentamicin. 100% sensitive to imipenem. All agents, except S. suis, are resistant to ampicillin. Agents resistant to ceftriaxon, ciprofloxacin and gentamicin have very high mortality (E. coli: 87.5% vs. 43.7%. S. suis : 66.7% vs. 25%). Conclusions: The most isolated agents are E. coli, K. pneumoniae and S. suis. Most of agents sensitive to ceftriaxon, ciprofloxacin và gentamicin. All agents, except S. suis, are resistant to ampicillin. Agents resistant to ceftriaxon, ciprofloxacin and gentamicin have very high mortality.

Relationship of antibiotic resistance to results of treatment in sepsis patients in Hue Central Hospital 2017 – 2018

2019 ◽  
pp. 48-54
Author(s):  
Duy Binh Nguyen ◽  
Trung Tien Phan ◽  
Trong Hanh Hoang ◽  
Van Tuan Mai ◽  
Xuan Chuong Tran
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Infection ◽  
Resistant Bacteria ◽  
Central Hospital ◽  
International Consensus ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Results Of Treatment ◽  
Amoxicillin Clavulanic Acid ◽  
Relationship Of

Sepsis is a serious bacterial infection. The main treatment is using antibiotics. However, the rate of antibiotic resistance is very high and this resistance is related to the outcome of treatment. Objectives: To evaluate the situation of antibiotic resistance of some isolated bacteria in sepsis patients treated at Hue Central Hospital; to evaluate the relationship of antibiotic resistance to the treatment results in patients with sepsis. Subjects and methods: prospective study of 60 sepsis patients diagnosed according to the criteria of the 3rd International Consensus-Sepsis 3 and its susceptibility patterns from April 2017 to August 2018. Results and Conclusions: The current agents of sepsis are mainly S. suis, Burkhoderiae spp. and E. coli. E. coli is resistant to cephalosporins 3rd, 4th generation and quinolone group is over 75%; resistance to imipenem 11.1%; the ESBL rate is 60%. S. suis resistant to ampicilline 11.1%; no resistance has been recorded to ceftriaxone and vancomycine. Resistance of Burkholderiae spp. to cefepime and amoxicillin/clavulanic acid was 42.9% and 55.6%, resistant to imipenem and meropenem is 20%, resistance to ceftazidime was not recorded. The deaths were mostly dued to E. coli and K. pneumoniae. The mortality for patients infected with antibiotic-resistant bacteria are higher than for sensitive groups. Key words: Sepsis, bacterial infection, antibiotics

scholarly journals Molecular Characterization of Shiga Toxin-ProducingEscherichia coliIsolated from Ruminant and Donkey Raw Milk Samples and Traditional Dairy Products in Iran

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Hassan Momtaz ◽  
Rahil Farzan ◽  
Ebrahim Rahimi ◽  
Farhad Safarpoor Dehkordi ◽  
Negar Souod
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Shiga Toxin ◽  
Dairy Products ◽  
Antibiotic Resistance Genes ◽  
Culture Method ◽  
Raw Milk ◽  
Antibiotics Resistance ◽  
E Coli ◽  
Pathogenic Genes

The aims of the current study were to detect the virulence factors and antibiotic resistance of Shiga toxin-producingE. coli, in animal milk and dairy products in Iran. AfterE. colidentification with culture method, PCR assay were developed for detection of pathogenic genes, serotypes and antibiotic resistance genes ofE. coli. Results showed that out of 719 samples, 102 (14.18%) were confirmed to be positive forE. coliand out of 102 positive samples, 17.64% were O26 and 13.72% were O157 and 1.96% were O91 and 1.96% were O145 serotypes. Totally, the prevalence ofstx1 andpapAgenes were the highest while the prevalence ofsfaSandfyuAwere the lowest in the positive samples. PCR results showed thattetA, tetBwere the highest (64.70%) andaac(3)-IVwere the lowest (27.45%) antibiotic resistant genes inE. colipositive samples. Our study indicated that the isolatedE. colitrains in these regions had a highest antibiotic resistance to tetracycline (58.82%) and the lowest to nitrofurantoin (3.92%).tetAgene andE. coliO157 serotype had highest andaac(3)-IVgene, andE. coliO145 serotype had a lowest frequency rates of antibiotics resistance genes, in the region.

Virulence factors analysis and antibiotic resistance of uropathogenic Escherichia coli isolated from patients in northeast of Iran

2020 ◽  
Author(s):  
Mahdis Ghavidel ◽  
Tahere Gholamhosseini-Moghadam ◽  
Kimiya Nourian ◽  
Kiarash Ghazvini
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
High Rate ◽  
Resistance Pattern ◽  
Antibiotics Resistance ◽  
Disk Diffusion Test ◽  
E Coli ◽  
Genes Encoding ◽  
Tract Infections

Background and Objectives: Escherichia coli is known to be the pathogen commonly isolated from those infected with uri- nary tract infections (UTIs). The aim of this study was to investigate the presence of E. coli virulence genes and antibiotics’ resistance pattern among clinical isolates in the Northeast of Iran. Relationships between virulence genes and antimicrobial resistances were studied as well. Materials and Methods: Three hundred isolates of E. coli were isolated from patients with UTIs that referred to Ghaem and Imam Reza hospitals (Mashhad, Iran) during August 2016 to February 2017. A multiplex PCR was employed to amplify the genes encoding pyelonephritis associated pili (pap), S-family adhesions (sfa), type1fimbriae (fimH) and aerobactin (aer). Disk diffusion test was performed to test the susceptibility of isolates to β-lactams, aminoglycosides, cephalosporins, quino- lone, fluoroquinolones, carbapenems and trimethoprim-sulfamethoxazole. Results: The PCR results identified the fimH in 78.4%, aer in 70.5%, sfa in 13.6% and the pap in 8.2% of isolates. The rates of antibiotic resistance of the isolates were as follows: 64.7% resistant to cephalosporins, 34% to trimethoprim-sul- famethoxazole, 31% to fluoroquinolones, 15.3% to aminoglycosides, 13.3% to β-lactams, 7.8% to quinolones and 4.4% to carbapenems. Significant relationships existed between pap and aer, pap and sfa, aer and fluoroquinolones also pap and cephalosporins. Conclusion: fimH and aer were found in > 50% of isolates suggesting the importance of both genes in UPEC. The majority of isolates had fimH as adhesion factor for colonization. Determining antibiotic resistance patterns in specific geographical areas is necessary for appropriate treatment of urinary tract infection. The high rate of resistance to cephalosporins is most likely due to incorrect drug administration

STUDY ON SOME CLINICAL, BIOCHEMICAL CHARACTERISTICS AND OUTCOME OF BACTERIAL MENINGITIS DUE TO STREPTOCOCCUS SUIS AT HUE CENTRAL HOSPITAL 2011-2012

2012 ◽  
pp. 46-51
Author(s):  
Xuan Chuong Tran ◽  
Quoc Dat Doan ◽  
Trung Tien Phan
Keyword(s):  
Infectious Disease ◽  
Bacterial Meningitis ◽  
Prospective Study ◽  
Key Words ◽  
Streptococcus Suis ◽  
Central Hospital ◽  
Biochemical Characteristics ◽  
Cranial Trauma ◽  
Study Results

Objectives: Bacterial meningitis is a very severe infectious disease in Vietnam. Studies about bacterial meningitis in our country in the last 10 years show that the most isolated causing bacteria is Streptococcus suis. Materials and methods: Prospective study. All the patients over 15 years old with diagnosis bacterial meningitis due to S. suis treated in Hue Central Hospital from May, 2011 to April, 2012 were recruited in this study. Results: 40 patients were enrolled in this study (male: 82.5%, female: 17.5%). Mean age 46.5 ± 17.72. 45% of patients had contact to pig or used pigs products. Related diseases: ENT diseases (25%), cranial trauma (5%), diabetes (7.5%), ect. The majority of patients have headache (97.5%), vomiting (85%), Kernig’s sign (72.5%), Brudzinsky’s sign (52.5%). 45% of patients have CRP 100 - 200 mg/L. 55% of patients have over 1000 cells/mm3 CSF, 47.5% of patients have CSF protein 3.0 ­– 5.0 g/L. In the result: 100% patients recovered, 40% had complications. Deefness was the most founded complication (37.5%). Conclusions: 45% of patients had contact to pig or used pigs products. The majority of patients have headache (97.5%), vomiting (85%), Kernig’s sign (72.5%), Brudzinsky’s sign (52.5%). Deefness was the most founded complication (37.5%). Key words: Meningitis, Streptococcus suis

scholarly journals Urine Microbial Landscape in Children with Urinary Tract Infections in Ryazan and Ryazan Region

Doctor Ru ◽  
2021 ◽  
Vol 20 (10) ◽  
pp. 48-53
Author(s):  
N.A. Belykh ◽  
◽  
S.V. Tereschenko ◽  
N.A. Anikeeva ◽  
S.S. Kantutis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Patient Specific ◽  
E Coli ◽  
Resistance Determinants ◽  
Study Results ◽  
Tract Infections

Study Objective: To study a spectrum of uropathogens and their sensitivity to antimicrobials in urinary tract infections (UTIs) in children in Ryazan and Ryazan Region. Study Design: retrospective study. Materials and Methods. We conducted a retrospective local laboratory monitoring of urinary microflora and analysed its sensitivity to antimicrobials in 111 patients aged 2 months to 17 years old who were undergoing traditional UI therapy in 2020. The study group comprised 75 (67.6%) girls and 36 (32.4%) boys. Pathogen isolation and type identification were performed using urine specimens collected in sterile disposable plastic containers prior to antimicrobial therapy. Material was delivered for analysis within 2 hours from collection. For testing of pathogen sensitivity to antimicrobials, we used the phenotyping diffusion test and an analytical test for carbapenems inactivation. Study Results. Prevailing causative agents of UIs were Escherichia coli (50.4%) and Klebsiella pneumoniae (14.4%). Resistance determinants were found in 9.0% and 2.7% of Е. соli and K. pneumoniae urological strains, respectively. The main mechanism of resistivity was production of wide spectrum plasmid β-lactamases. The highest activity in E. coli was demonstrated by generation III–IV cephalosporins, aminoglycosides, fosfomicin (100%), nitrofurantoin (91.3%), and aminopenicillins (76.1–86.9%). For K. pneumoniae, generation III–IV cephalosporins and aminoglycosides were most potent (100%). All resistant pathogens were sensitive to cefoperazone sulbactam, meropenem, imipenem, aminoglycosides (100%); tigecycline, nitrofurantoin, and fosfomicin were most potent against E. coli. Conclusion. Children with UIs in Ryazan Region had mostly gram-negative bacteria in their urine (85.6%), Enterobacteralеs (81.1%) being a prevailing type. Antimicrobials resistance determinants were quite rare (17.8%) in these urine isolates; all of them were class A ЕSBL producers. These characteristic features of antibiotic resistance of uropathogenic enterobacteria strains allow using β-lactam antibiotics in empiric initial treatment and emphasising the need in patient-specific selection of antimicrobials. Keywords: antibacterial therapy, antibiotic resistance, children, urinary tract infections, Escherichia coli, Klebsiella pneumoniae.

scholarly journals Phenotypic and Genotypic Study of Antibiotics Resistance Profile in Escherichia coli Isolated from Broilers in Cianjur, Indonesia

2021 ◽  
Vol 9 (2) ◽  
pp. 97-104
Author(s):  
Aprilia Hardiati ◽  
Safika ◽  
I Wayan Teguh Wibawan ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistant Isolate ◽  
Diffusion Method ◽  
Future Research ◽  
Antibiotics Resistance ◽  
Isolation And Identification ◽  
Resistance Profile ◽  
E Coli ◽  
Encoding Gene

This study aimed to investigate the phenotypic and genotypic of antibiotics resistance profile in Escherichia coli. The 30 samples come from cloacal swab of broilers in Cianjur, Indonesia. Isolation and identification of E. coli was performed by culture in McConkey agar, eosin methylene blue agar, Gram staining and five essential biochemical tests (IMViC). In this study, 10 isolates (33.3%) were confirmed E. coli positive. Phenotypic profile was performed by screening all isolates with 8 antibiotics of 6 antibiotic groups. The screening was carried by Kirby-Bauer disk diffusion method based on the standard of CLSI. For genotypic profile, each resistant isolate was detected antibiotic resistance-encoding gene. The result showed all isolates (100%) resistant against tetracyclin, oxytetracycline and erythromycin. Nine isolates (90%) detected nalidixic acid and enrofloxacin-resistant. The ciprofloxacin and gentamicin-resistant isolates were 70% and 40%, respectively. There was no resistant isolate for chloramphenicol. Multi drug-resistant was detected on 90% isolates. Only gyrA (100%) and tetA (80%) genes were detected. This study showed high rate of occurrence of antibiotic resistance in E. coli. Not all resistant isolates were detected in the antibiotic resistance-encoding gene in this study. Future research to detect resistance genes should use more varied target genes.

scholarly journals ASSESSMENT OF ANTIBIOTIC RESISTANCE AND BACTERIAL CONTAMINATION OF ICE SOLD IN CANTHO CITY, VIETNAM

2019 ◽  
Vol 57 (3B) ◽  
pp. 49
Author(s):  
Ngoc Thi Anh Tong
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Contamination ◽  
Antibiotic Resistance Gene ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Can Tho City ◽  
The City

This study aimed to investigate the bacterial contamination of flake and cube ice being used dailyin the community. Thirty-one ice samples were collected from different areas in the city of Can Tho city, Vietnam. The enumeration of total aerobic mesophilic counts, the presence of coliforms and Escherichia coli (E. coli) and determination of antibiotics resistance of E. coli isolates were examined. The results indicated that total aerobic mesophilic counts ranged from 2.5 to 6.2 log CFU/mL and significant differences of total aerobic mesophilic counts were found between flake ice and cube ice (p < 0.05). Coliforms and E. coli were present on the ice samples of 93.55% and 58.06%, respectively. A total of 39 E. coli isolates were tested their resistance to 15 different antibiotics. The E. coli isolates of 74.36% were multi-resistance from three to thirteen antibiotics. The high prevalance was resistant to Ampicillin (79.49%), Cefotaxime (69.23%), Ceftazidime (46.15%), Tetracycline (56.41%), Sulfamethoxazole/Trimethoprime (46.15%), Colistin (20.51%), etc. As E. coli is an hygiene indicator and a candidate vehicle for the transfer of antibiotic resistance gene, it is highly recommended using clean and probable water in ice making as well as preventing the spread of antibiotic resistant bacteria.

scholarly journals Raw Milk in Noakhlai, Bangladesh: Quality Assessment and Antibiotic Resistance of Identified Microorganisms

2021 ◽  
Vol 9 (3) ◽  
pp. 1104-1112
Author(s):  
Tahmina Akter ◽  
Mansura Mokbul ◽  
Susmita Ghosh ◽  
Moumita Dey
Keyword(s):  
Antibiotic Resistance ◽  
Age Groups ◽  
Resistance Index ◽  
Raw Milk ◽  
Antibiotics Resistance ◽  
Bacterial Isolation ◽  
E Coli ◽  
Milk Samples ◽  
Micro Organisms ◽  
High Treatment

Milk is an ideal food for all age groups. The current study was carried out to identify the microorganisms to assess the raw milk quality and the antibiotic resistance of those identified micro-organisms. Five raw milk samples along with two high treatment (UHT) milk samples from different locations of Noakhali district of Bangladesh were analysed. Bacterial isolation was performed by Nutrient Agar (NA) and MacConkey (MCA), Eiosin Methylene Blue (EMB) and Genital menital salt agar (GMSA). The isolates were then identified by Kliger’s Iron Agar (KIA) test, Motility Indole Urease (MIU) test, Catalase and Oxidase tests. Antibiotics resistance tests were done for 13 different antibiotics. Among all these samples, Maijdee Bazar (S4) contained the highest load as 1.87×106 and the UHT samples contained no bacterial contamination. E. coli covered 47.05% whereas Listeria, Bacillus and Yersinia were in the same percentage as 5.88% among all isolates. Salmonella and Staphylococcus were 23.53% and 11.76%, respectively. Listeria and Salmonella were resistant to five different antibiotics by 46.15% and 38.46% of multiple antibiotic resistance index (MRI), correspondingly. However, E. coli and Yersinia were resistant to three antibiotics namely, Rifampcin (RIF), Cefotaxime (CTX), Amoxycillin (AMX) by about 23% as MRI percentage. Bacillus and Staphylococcus both were resistant to Cefepime (CPM) by 7.69% of MRI. Hence, it can be concluded that Rifampcin and Cefepime were most common antibiotics which were resisted by most of the isolates. Therefore, hygiene aspect of these milk sources needs to be taken into consideration with high priority. Also, the antibiotics which are resisted by different organisms will be detrimental for public health aspects.

scholarly journals Treatment of urinary tract infections in condition of growing antibiotic resistance: Antimicrobial Stewardship program

2019 ◽  
pp. 62-71
Author(s):  
O.I. Chub ◽  
O.V. Bilchenko ◽  
O.M. Godlevska ◽  
S.V. Teslenko
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Stewardship ◽  
Third Generation ◽  
Antimicrobial Stewardship Program ◽  
E Coli ◽  
Stewardship Program ◽  
The Right ◽  
Tract Infections

 Resistance to common groups of antibiotics has been increasing in the treatment of urinary tract infections worldwide. In the United States, CDC has estimated that more thаn 2 million infections and 23,000 deaths are due to antibiotic resistance each year. In Europe, an еstimated 25,000 deaths are attributable to antibiotic-rеsistant infections. By 2050, it is estimated that antibiotic resistance will cause 10 million deaths every year.At the EU/EEA level, more than half (58.2%) of the E. coli isolates reported to EARS-Net for 2017 were resistant to at least one of the antimicrobial groups under regular surveillance, i.e. aminopenicillins, fluoroquinolones, third-generation cephalosporins, aminoglycosides and carbapenems. A majority (87.4%) of the third-generation cephalosporin-resistant E. coli isolates from 2017 were extended-spectrum beta-lactamase (ESBL)-positive. Use of broad-spectrum antimicrobials is a known risk factor for colonization and spread of resistant Enterobacteriaceae, including E. coli. The high levels of ESBLs and increasing resistance to key antimicrobial groups might also lead to an increased consumption of carbapenems, which in turn can increase the selection pressure and facilitated the spread of carbapenem-resistant Enterobacteriaceae. According to annual reports of CDC, WHO, EARS-Net and others,estimate that 30 percent of all antibiotics prescribed in outpatient clinics and 40 percent of all antibiotics prescribed in inpatient clinics were unnecessary. Improving the way we use antibiotics, often referred to as «antibiotic stewardship», is part of the National Action Plan. Appropriate antibiotic use means using the right antibiotic, at theright dose, for the right duration, and at the right time. The article reflects the recommendations of the European Association of Urology 2019 on the management and treatment of urinary tract infections in accordance with the principles of Antimicrobial Stewardship Program.

scholarly journals Genetic Characterization of Multiple Antibiotics Resistance Genes of Escherichia coli Strain from Cow Milk and Its Products Sold in Abuja, Nigeria

2020 ◽  
pp. 40-50
Author(s):  
E. C. Okechukwu ◽  
E. U. Amuta ◽  
G. M. Gberikon ◽  
N. Chima ◽  
B. Yakubu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Multidrug Resistant ◽  
Coli Strain ◽  
Cow Milk ◽  
Animal Origin ◽  
Resistance Pattern ◽  
Antibiotics Resistance ◽  
E Coli ◽  
Food Pathogens

Background: Antibiotic resistance has remained a global concern. Food pathogens that carry resistance genes will cause public health threat irrespective of their pathogenicity, as this pool of resistance genes are disseminated via food chain. The antibiotic susceptibility of E. coli isolates to different antibiotics were investigated and resistance genes were genetically identified by multiplex Polymerase Chain Reaction (PCR). The study aimed to determine the phenotypic antibiotic resistance pattern of the E. coli isolates and characterize the antibiotics resistance genes in the E. coli isolates from the Cow milk and milk products. Results: The study showed that the E. coli isolates were Multidrug resistant (MDR) to several antibiotics with resistance pattern of four to ten combinations of antibiotics and the antibiotics resistance occurred mostly in three classes of antibiotics, namely: β-lactams, Amphenicol and Tetracycline.   The antibiotics resistance genes  expressed in the multidrug resistant E. coli isolates were tetA 22(78.6%), dfrA1 9(32.1%), blaCITM and blaSHV 3(10.7%), tetB and blaTEM 1(3.6%), qnr 0(0%) and aac (3)-iv 0(0%). Conclusion: The study showed that the cow milk and its products sold on the streets of Abuja are contaminated with resistant E. coli that can pose health risk to the consumers.  Monitoring and screening for resistant food pathogens in food especially of animal origin is vital to mitigate the prevalence of antibiotics resistance.

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