Un Gecko africain Hemidactylus mabouia (Squamata, Gekkonidae) dans la lutte contre les fausses teignes des ruches Galleria mellonella et Achroia grisella (Lepidoptera, Pyralidae)

2009 ◽  
Vol 114 (4) ◽  
pp. 423-427
Author(s):  
Aziz Dridah ◽  
Kamel Louadi ◽  
Selima Berchi
Keyword(s):  
Galleria Mellonella ◽  
Achroia Grisella ◽  
Lepidoptera Pyralidae ◽  
Hemidactylus Mabouia

Assessing the Efficacy of a Product Containing Bacillus thuringiensis Applied to Honey Bee (Hymenoptera: Apidae) Foundation as a Control for Galleria mellonella (Lepidoptera: Pyralidae)

2009 ◽  
Vol 44 (2) ◽  
pp. 158-163 ◽  
Author(s):  
Amanda M. Ellis ◽  
G. W. Hayes
Keyword(s):  
Bacillus Thuringiensis ◽  
Honey Bee ◽  
Galleria Mellonella ◽  
Toxic Chemicals ◽  
Larval Feeding ◽  
Achroia Grisella ◽  
Significant Damage ◽  
Lepidoptera Pyralidae ◽  
Financial Losses ◽  
Wax Moth

Greater (Galleria mellonella L.) and lesser wax moth (Achroia grisella F.) larval feeding can cause significant damage in active honey bee (Apis mellifera L.) colonies and stored equipment. This damage may lead to significant material and financial losses. Traditional control methods use toxic chemicals that may leave residues in wax and honey and are potentially hazardous to bees and humans. In this study, we evaluated the use of a product (B401®; Vita [Europe] Ltd., Basingstoke, UK) that contains Bacillus thuringiensis Berliner, a bacterium that is not harmful to bees or humans. The main objectives of our research were to determine if B401 could be successfully applied to the midrib of foundation during its production and if combs constructed on the foundation were protected from wax moth damage. B401 significantly reduced the comb damage score that was given to combs on a scale from 0–10, with 0 equal to no damage and 10 equal to complete damage (B401 = 1.70 ± 0.39 and 0.45 ±0.16, control = 8.55 ± 0.32 and 3.80 ± 0.71) and the proportion of larvae surviving at 6 wk (B401 = 0.69 = 0.07, control = 0.95 ± 0.04). Yet, as administered in our study, the product did not eliminate all wax moth damage to combs.

INFLUÊNCIA DO SUBSTRATO E DA LUMINOSIDADE NA INFECÇÃO DE NEMATOIDES ENTOMOPATOGÊNICOS EM Galleria mellonella (LEPIDOPTERA: PYRALIDAE)

2018 ◽  
Vol 20 (2) ◽  
pp. 91-101
Author(s):  
Andressa Lima de Brida ◽  
Silvia Renata Siciliano Wilcken ◽  
Luis Garrigós Leite
Keyword(s):  
Galleria Mellonella ◽  
Steinernema Carpocapsae ◽  
Steinernema Feltiae ◽  
Lepidoptera Pyralidae

Nematoides entomopatogênicos (NEPs) são alternativas eficientes para o controle de pragas. O emprego de novas técnicas da produção in vivo, permite o progresso da tecnologia de formulação de bioinseticidas. O objetivo do trabalho, foi avaliar a influência da luminosidade e do substrato na capacidade de infecção de juvenis infectantes (JIs) de Steinernema brazilense IBCBn 06, Steinernema carpocapsae IBCBn 02, Steinernema feltiae IBCBn 47 e Heterorhabditis amazonensis IBCBn 24 em lagartas de Galleria mellonella (Lepidoptera: Pyralidae). O delineamento experimental foi inteiramente casualizado com quatro tratamentos e oito repetições. As parcelas, constituídas por placa de Petri com, substrato-areia e substrato-papel filtro, com e sem luminosidade, inoculados com suspensão de 1,5 mL contendo 400JIs e quatro lagartas de G. mellonella. O número de JIs foi quantificado após a mortalidade das lagartas. A taxa de infecção de JIs de S. carpocapsae IBCBn 02 e S. feltiae IBCBn 47 variaram de 2,14 a 3,28 e de 11,04 a 13,09 JIs/lagarta. O substrato-areia com e sem luminosidade permitiu a maior taxa de infeção dos JIs de S. brazilense IBCBn 06 de 7,86 e 9,44 JIs/lagarta, e 13,49 JIs/lagarta com luminosidade para H. amazonensis IBCBn 24. O substrato-areia, permite a maior taxa de infecção por JIs de NEPs.

Penetration and encapsulation of the larval endoparasitoid Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae)

2013 ◽  
Vol 104 (2) ◽  
pp. 203-212 ◽  
Author(s):  
A. Valigurová ◽  
V. Michalková ◽  
P. Koník ◽  
M.L. Dindo ◽  
M. Gelnar ◽  
...  
Keyword(s):  
Galleria Mellonella ◽  
Larval Instar ◽  
Posterior Part ◽  
Immunological Response ◽  
Dark Spot ◽  
Foreign Objects ◽  
Factitious Host ◽  
Lepidoptera Pyralidae ◽  
Cellular Defence ◽  
Host Tissues

AbstractThe tachinid fly Exorista larvarum (L.) (Diptera: Tachinidae) is a polyphagous larval endoparasitoid that deposits its eggs on the host exoskeleton of lepidopteran and tenthredinid larvae. The attachment of larval E. larvarum and the formation of the respiratory funnel were studied during infestation in the last larval instar of the wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). The tachinid larvae burrow through the host integument after hatching, using their robust cephalopharyngeal skeleton, leaving a dark spot at the point of their penetration as a result of host cuticle melanization. Endoparasitoid penetration induces the host cellular defence, resulting in the formation of a haemocyte capsule consisting of multi-cellular sheaths. This enveloping capsule later undergoes melanization, which is mostly obvious towards the posterior part of the endoparasitoid. The endoparasitoid uses the host encapsulation response to build a respiratory funnel from the modified host integument, leading to the host surface. The encapsulated larva remains attached to the respiratory funnel via an anal hook and cuticular spines until fully developed. Additional immunohistochemical analyses were used to study host–parasitoid interactions. Indirect immunofluorescence showed no labelling of potential tachinid antigens and confirmed no effect on the surrounding host tissues. A simulated parasitization with coated polybead microspheres revealed the mortal impact of tachinid antigens to the host. Hosts injected with antigen-coated polybeads died as a consequence of an acute and extensive immunological response to the tachinid antigens and not due to the trauma caused by foreign objects inside their body.

Greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) as a resistant model host for Nosema pyrausta (Microsporidia: Nosematidae)

2018 ◽  
Vol 157 ◽  
pp. 1-3 ◽  
Author(s):  
Yuri S. Tokarev ◽  
Ekaterina V. Grizanova ◽  
Anastasia N. Ignatieva ◽  
Ivan M. Dubovskiy
Keyword(s):  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Lepidoptera Pyralidae ◽  
Wax Moth
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