Infant formula and adult nutritionals. Determination of ?-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)

2020 ◽  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Infant Formula ◽  
High Performance ◽  
Reversed Phase

scholarly journals Determination of Biotin by High-Performance Liquid Chromatography in Infant Formula, Medical Nutritional Products, and Vitamin Premixes

2006 ◽  
Vol 89 (6) ◽  
pp. 1515-1518 ◽  
Author(s):  
Linda B Thompson ◽  
Daniel J Schmitz ◽  
Shang-Jing Pan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Infant Formula ◽  
High Performance ◽  
Solid Phase ◽  
Reversed Phase ◽  
Hplc Method ◽  
Fluorescence Detector ◽  
Sample Preparation Procedure

Abstract A solid-phase extraction sample preparation procedure was developed for use with a high-performance liquid chromatography (HPLC) method for biotin analysis. The HPLC method used a reversed-phase C18 column; chromatography run time was 8.5 min. After eluting from the column, biotin went through postcolumn reaction to form a conjugate with streptavidinfluorescein isothiocyanate, which was then detected by a fluorescence detector. This method was tested with infant formula, medical nutritional products, and vitamin premix samples.

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