Foodstuffs - Multimethod for the screening of aflatoxin B1, deoxynivalenol, fumonisin B1 and B2, ochratoxin A, T-2 toxin, HT-2 toxin and zearalenone in foodstuffs, excluding foods for infants and young children, by LC-MS/MS

2019 ◽  
Keyword(s):  
Young Children ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Infants And Young Children

scholarly journals Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 415 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Yun Fang ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Goodness Of Fit ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Quantitative Detection ◽  
Antibody Microarray ◽  
Test Kit ◽  
Microarray Immunoassay

We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

Dietary exposure and health risk characterization of aflatoxin B1, ochratoxin A, fumonisin B1, and zearalenone in food from different provinces in Northern Vietnam

Food Control ◽  
2020 ◽  
Vol 112 ◽  
pp. 107108 ◽  
Author(s):  
Tuan Huu Do ◽  
Son Cao Tran ◽  
Chi Dinh Le ◽  
Ha-Binh Thi Nguyen ◽  
Phuong-Thao Thi Le ◽  
...  
Keyword(s):  
Health Risk ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Dietary Exposure ◽  
Risk Characterization ◽  
Northern Vietnam

scholarly journals Co-occurrence of aflatoxin B1, fumonisin B1, ochratoxin A and zearalenone in cereals and peanuts from Côte d’Ivoire

2006 ◽  
Vol 23 (10) ◽  
pp. 1000-1007 ◽  
Author(s):  
Béatrice Sangare-Tigori ◽  
Serge Moukha ◽  
H. James Kouadio ◽  
Anne-Marie Betbeder ◽  
Djédjé Sébastien Dano ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Cote D'ivoire ◽  
Aflatoxin B1 ◽  
Côte D’Ivoire ◽  
Fumonisin B1 ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

scholarly journals Risk of exposure to aflatoxin B1, ochratoxin A, and fumonisin B1 from spices used routinely in Lebanese cooking

2021 ◽  
Vol 147 ◽  
pp. 111895
Author(s):  
Manar Al Ayoubi ◽  
Michele Solfrizzo ◽  
Lucia Gambacorta ◽  
Ian Watson ◽  
Nada El Darra
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Risk Of Exposure

scholarly journals Fluorescence Enhancement on Silver-Plated Plasma Micro-Nanostructured 3D Polymeric Microarray Substrates for Multiplex Mycotoxin Detection

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 392
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Anastasia Kanioura ◽  
Katerina Tsougeni ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fluorescence Intensity ◽  
Aflatoxin B1 ◽  
Oxygen Plasma ◽  
Fluorescence Enhancement ◽  
Fumonisin B1 ◽  
Silver Deposition ◽  
Standard Glass ◽  
Glass Slides ◽  
Coefficients Of Variation

Oxygen plasma micro-nanostructured poly(methyl methacrylate) (PMMA) slides were modified through silver microparticle deposition to create microarray substrates that enhance the emitted fluorescence intensity. Silver deposition relied on a commercially available reagent and was completed in two 30-min incubation cycles of the substrate with the reagent. The fluorescence enhancement achieved using these substrates over flat PMMA slides was determined through the development of a microarray for the multiplexed detection of four mycotoxins, aflatoxin B1, ochratoxin A, fumonisin B1, and deoxynivalenol. It was shown that the implementation of silver-plated oxygen plasma micro-nanotextured PMMA substrates increased the signals obtained for aflatoxin B1 and ochratoxin A by approximately 2.8 times, 5.6 times for deoxynivalenol, and 16-times for fumonisin B1, compared to flat PMMA substrates. Most notably, this signal increase was not accompanied by a significant increase in the non-specific signal. In addition, the spot repeatability both across a single slide as well as between different slides was high, with coefficients of variation lower than 12%. The slides were also stable for at least three months, thus offering a microarray substrate with improved properties compared to standard glass slides, regarding both the absolute spot fluorescence intensity and between spots repeatability.

scholarly journals Bioavailability of aflatoxin B1 and ochratoxin A, but not fumonisin B1 or deoxynivalenol, is increased in starch-induced low ruminal pH in nonlactating dairy cows

2016 ◽  
Vol 99 (12) ◽  
pp. 9759-9767 ◽  
Author(s):  
D. Pantaya ◽  
D.P. Morgavi ◽  
M. Silberberg ◽  
F. Chaucheyras-Durand ◽  
C. Martin ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Ruminal Ph
Sign in / Sign up

Export Citation Format

Share Document