Radiological protection. Performance criteria for laboratories using the cytokinesis block micronucleus (CBMN) assay in peripheral blood lymphocytes for biological dosimetry

2018 ◽  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Performance Criteria ◽  
Radiological Protection ◽  
Biological Dosimetry ◽  
Blood Lymphocytes ◽  
Cbmn Assay

Cytogenetic analysis of peripheral blood lymphocytes of hospital staff occupationally exposed to low doses of ionizing radiation

2010 ◽  
Vol 26 (5) ◽  
pp. 273-280 ◽  
Author(s):  
Ayşe Eken ◽  
Ahmet Aydın ◽  
Onur Erdem ◽  
Cemal Akay ◽  
Hatice Tuba Sanal ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Peripheral Blood ◽  
Chromosome Instability ◽  
Hospital Staff ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Blood Lymphocytes ◽  
Significant Difference ◽  
Cbmn Assay ◽  
Occupationally Exposed

Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis. The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose ionizing radiation in comparison with a selected control group, by using the cytokinesis-blocked micronucleus (CBMN) and sister chromatid exchange (SCE) tests in peripheral blood lymphocytes. The study included 40 exposed radiology staff and 30 control subjects. The frequency of micronuclei (MN) was significantly increased in radiation-exposed groups compared with control persons (p < 0.05). The frequency of SCE did not show any significant difference in the exposed individuals in comparison to the controls. Our results showed that low-level chronic occupational exposure to ionizing radiation causes an increase of MN frequency in chromosomes, even though the absorbed doses were below the permissible limits. Our studies indicate that the CBMN assay is considered to be sensitive test in contrast to SCE analysis to evaluate chromosomal damage induced by ionizing radiation.

scholarly journals Biological Dosimetry Using Human Interphase Peripheral Blood Lymphocytes

2002 ◽  
Vol 167 (suppl_1) ◽  
pp. 10-12 ◽  
Author(s):  
Pataje G.S. Prasanna ◽  
Christine J.C. Hamel ◽  
Nestor D. Escalada ◽  
Keife L. Duffy ◽  
William F. Blakely
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Biological Dosimetry ◽  
Blood Lymphocytes
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