Electrically operated blood storage refrigerators

AbstractAcute normovolemic hemodilution (ANH) is a potential transfusion method for platelets, as well as for red blood cells. However, previous studies have shown that whole blood storage in ANH decreases platelet aggregability by 14.7–76.3% and that this decrease is not recovered by reinfusion. We investigated whether a new whole blood storage method for 6 h using a polyolefin bag, based on the platelet concentrates storage method, would maintain platelet function better than the conventional method using a polyvinyl chloride bag. We demonstrated that storage of whole blood in a polyolefin bag maintained ADP-induced aggregation rates at more than twofold higher than those in a polyvinyl chloride bag, and also significantly suppressed P-selectin expression, a platelet activation marker (ADP-induced aggregation rates: 24.6 ± 5.1% vs. 51.7 ± 11.5%, p = 0.002; P-selectin expression; 50.3 ± 8.4MFI vs. 31.6 ± 9.3MFI, p = 0.018). These results could be attributed to the high gas permeability of polyolefin, which lowered PCO2 and maintained a high pH with or without agitation. There were no significant changes in platelet count and red blood cell parameters due to the storage methods. Our results suggest that ANH using polyolefin bags is advantageous in improving hemostatic function compared to the conventional method.

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Maternal understanding of commercial cord blood storage for their offspring - a survey among pregnant women in Hong Kong

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.1111/j.1600-0412.2011.01201.x ◽

2011 ◽

Vol 90 (9) ◽

pp. 1005-1009 ◽

Cited By ~ 7

Author(s):

STEPHEN SIK HUNG SUEN ◽

TERENCE T LAO ◽

OI KA CHAN ◽

THOMAS KAM ON KOU ◽

SAMMY CHUNG SUM CHAN ◽

...

Keyword(s):

Hong Kong ◽

Cord Blood ◽

Pregnant Women ◽

Blood Storage

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Autologous blood storage before hepatectomy for hepatocellular carcinoma with underlying liver disease

British Journal of Surgery ◽

10.1002/bjs.4012 ◽

2003 ◽

Vol 90 (1) ◽

pp. 23-28 ◽

Cited By ~ 30

Author(s):

T. Itamoto ◽

K. Katayama ◽

H. Nakahara ◽

H. Tashiro ◽

T. Asahara

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Disease ◽

Autologous Blood ◽

Blood Storage ◽

Underlying Liver Disease

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The effect of blood storage duration on in-hospital mortality: a randomized controlled pilot feasibility trial

Transfusion ◽

10.1111/j.1537-2995.2011.03521.x ◽

2012 ◽

Vol 52 (6) ◽

pp. 1203-1212 ◽

Cited By ~ 48

Author(s):

Nancy M. Heddle ◽

Richard J. Cook ◽

Donald M. Arnold ◽

Mark A. Crowther ◽

Theodore E. Warkentin ◽

...

Keyword(s):

Hospital Mortality ◽

Feasibility Trial ◽

Blood Storage ◽

Storage Duration ◽

Randomized Controlled

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Use of antioxidant nanoparticles to reduce oxidative stress in blood storage

Biotechnology and Applied Biochemistry ◽

10.1002/bab.2240 ◽

2021 ◽

Author(s):

Saeid Barzegar ◽

Amir Asri Kojabad ◽

Rima Manafi Shabestari ◽

Mehdi Barati ◽

Mohammad Reza Rezvany ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Storage

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Changes in the microRNA expression profile during blood storage

BMJ Open Sport & Exercise Medicine ◽

10.1136/bmjsem-2018-000354 ◽

2018 ◽

Vol 4 (1) ◽

pp. e000354 ◽

Cited By ~ 2

Author(s):

Anna Haberberger ◽

Benedikt Kirchner ◽

Irmgard Riedmaier ◽

Reinhard Henschler ◽

Christian Wichmann ◽

...

Keyword(s):

Blood Donation ◽

Autologous Blood ◽

Blood Storage ◽

Fresh Blood ◽

Biomarker Signature ◽

Male Subjects ◽

Stored Blood ◽

Individual Erythrocytes ◽

Generation Sequencing

ObjectivesFor several decades, autologous blood doping (ABD) in sports has been a major problem, and even today there is still no reliable method for satisfactorily detecting ABD. For this kind of doping, stored individual erythrocytes are used to increase stamina and endurance caused by a higher erythrocyte level in the athlete’s body. Since there is growing evidence that these cells are enriched with microRNAs (miRNAs), this study has been carried out to discover and validate all miRNAs occurring in fresh blood as well as in stored blood.MethodsTherefore, small RNA Next Generation Sequencing has been performed, which allows untargeted detection of all miRNAs in a blood sample. The focus of this investigation has been to find miRNA alterations in blood bags after erythrocyte processing and during storage, as compared with fresh blood directly withdrawn from subjects. Blood samples were obtained from 12 healthy, recreationally active male subjects three times before blood donation and from blood bags at several time points after blood processing.Results189 miRNAs have been considered stable over two consecutive weeks. A further analysis revealed a complex biomarker signature of 28 miRNAs, consisting of 6 miRNAs that altered during 6 weeks of storage and 22 miRNAs that altered due to processing.ConclusionThese results suggest that the identified miRNA biomarker signature may be used for the detection of ABD. These 28 miRNA candidates are tested and verified currently in a follow-up study, a human transfusion clinical trial in healthy sportsmen.

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