Methods of testing plastics. Determination of butyl rubber content of low density polythene compounds

1979 ◽  
2005 ◽  
Vol 12 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Gabriel Virella ◽  
M. Brooks Derrick ◽  
Virginia Pate ◽  
Charlyne Chassereau ◽  
Suzanne R. Thorpe ◽  
...  

ABSTRACT Antibodies to malondialdehyde (MDA)-modified low-density lipoprotein (LDL), copper-oxidized LDL (oxLDL), N ε(carboxymethyl) lysine (CML)-modified LDL, and advanced glycosylation end product (AGE)-modified LDL were obtained by immunization of rabbits with in vitro-modified human LDL preparations. After absorption of apolipoprotein B (ApoB) antibodies, we obtained antibodies specific for each modified lipoprotein with unique patterns of reactivity. MDA-LDL antibodies reacted strongly with MDA-LDL and also with oxLDL. CML-LDL antibodies reacted strongly with CML-LDL and also AGE-LDL. oxLDL antibodies reacted with oxLDL but not with MDA-LDL, and AGE-LDL antibodies reacted with AGE-LDL but not with CML-LDL. Capture assays were set with each antiserum, and we tested their ability to capture ApoB-containing lipoproteins isolated from precipitated immune complexes (IC) and from the supernatants remaining after IC precipitation (free lipoproteins). All antibodies captured lipoproteins contained in IC more effectively than free lipoproteins. Analysis of lipoproteins in IC by gas chromatography-mass spectrometry showed that they contained MDA-LDL and CML-LDL in significantly higher concentrations than free lipoproteins. A significant correlation (r = 0.706, P < 0.019) was obtained between the MDA concentrations determined by chemical analysis and by the capture assay of lipoproteins present in IC. In conclusion, we have developed capture assays for different LDL modifications in human ApoB/E lipoprotein-rich fractions isolated from precipitated IC. This approach obviates the interference of IC in previously reported modified LDL assays and allows determination of the degree of modification of LDL with greater accuracy.


1986 ◽  
Vol 26 (6) ◽  
pp. 885-889
Author(s):  
L. V. Al'tshuler ◽  
G. S. Doronin ◽  
S. V. Klochkov
Keyword(s):  

2012 ◽  
Vol 46 (7) ◽  
pp. 2140-2148 ◽  
Author(s):  
Yanyan Zhang ◽  
Lela K. Riley ◽  
Mengshi Lin ◽  
Zhiqiang Hu

2021 ◽  
Author(s):  
Ying Chen ◽  
Dong Yiyang ◽  
Xiang Ma ◽  
Jiaru Li ◽  
Minmin Guo ◽  
...  

Abstract Background: Taraxacum kok-saghyz (TKS), a plant native to the Tianshan valley on the border between China and Kazakhstan and inherently rich in natural rubber, inulin and other bioactive ingredients, is an important industrial crop. TKS rubber is a good substitute for natural rubber. TKS's breeding work necessitates the need to screen high-yielding varieties, hence rapid determination of rubber content is essential for the screening. Conventional analytical methods cannot meet actual needs in terms of real-time testing and economic cost. Near-infrared spectroscopy analysis technology, which has developed rapidly in the field of industrial process analysis in recent years, is a green detection technology with obvious merits of fast measurement speed, low cost and no sample loss. This research aims to develop a portable non-destructive near-infrared spectroscopic detection scheme to evaluate the content of natural rubber in TKS fresh roots. Pyrolysis gas chromatography (PyGC), was chosen as the reference method for the development of NIR prediction model. Results: 208 TKS fresh root samples were collected from the Inner Mongolia Autonomous Region of China. Near-infrared spectra were acquired for all samples. Randomly two-thirds of them were selected as the calibration set, the remaining one-third as the verification set, and the partial least squares method was successfully used to establish a good NIR prediction model at 1080-1800nm with a performance to deviation ratio (RPD) of 5.54 and coefficient of determination (R2) of 0.95. Conclusions: This study showed that portable near-infrared spectroscopy could be used with ease for large-scale screening of TKS plants in farmland, and could greatly facilitate TKS germplasm preservation, high-yield cultivation, environment-friendly, high-efficiency and low-cost rubber extraction, and comprehensive advancement of the dandelion rubber industry thereof.


Author(s):  
Diarrassouba Moussa ◽  
Soumahin Eric Francis ◽  
Konan Djézou ◽  
N’guessan Kan Pulchérie ◽  
Moro Affia Perpetue ◽  
...  

To find out the effect of cultural techniques on agrophysiological parameters, four combinations ̏planting densities (low density or DF at 350 t/ha, normal density or DN at 510 t/ha) and opening standards (opening at 65cm and opening at 50 cm) ̋ were tested on Hevea brasiliensis clones PB 260, IRCA 111 and RRIM 703. This study was conducted for nine years with a split-plot experimental design of two treatments and two subtreatments repeated three times. The different combinations of treatments and subtreatments tested were low density (DN-50 cm), low density (DN-65 cm), normal density (DF-50 cm), normal density (DF- 65 cm). The parameters evaluated were the production at bleeding and per hectare, the average annual increase in circumference, the rate of tree losses and the rate of trees with dry notches. Production per tree was significantly higher at 350 t/ha and 65 cm opening (DF-65 cm), while per hectare production was higher at 510 t/ha. The girths of the different clones are stronger at DF and at the 65 cm opening. The rate of tree loss and the rate of trees with dry notch were low at the 510 t/ha density and the 65cm opening. The appropriate density and opening standard was "normal density 510 t/ha and opening to circumference 50 cm". The cultivation techniques especially the density and opening standard judiciously applied can participate in the sustainable improvement of rubber productivity of rubber plantations.


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