Sterilization of health care products. Microbiological methods

2018 ◽  
2021 ◽  
Vol 18 (4) ◽  
pp. 93-98
Author(s):  
A. I. Zhabrouskaya ◽  
O. A. Emeliyanova ◽  
N. V. Dudchik

Objective. To assess internal environment objects ofsecond cleanliness class health care facilities according to microbiological standards.Materials and methods. The methods of swabbing, direct seeding, membrane filtration and instrumental aspiration were used for sampling. The microbial status was analyzed by cultural and biochemical methods on nutrient, differential and diagnostic media with species identification using the microbiological analyzer. The phenotypic features were studied in vitro by the standard biochemical and microbiological methods in accordance with the principles of good laboratory practice.Results. The microbiological testing of indoor air and internal environment objects of second cleanliness class health care facilities (dental offices) was done to determine the qualitative and quantitative composition of the microbiota. As a result of the taxonomic identification, it has been found that the most common representatives of the air microbiota are Staphylococcus, Micrococcus and Kocuria bacteria, which are true residents of the human dermis.Conclusion. The obtained data provide material for the study of the phenomenon of the modification of phenotypic properties and its use at the stages of hazard detection and profiling and for the minimization of uncertainty within the concept of microbial risk analysis.


2021 ◽  
Vol 22 (3) ◽  
pp. 203-210
Author(s):  
Y. Ya'aba ◽  
S.B. Mohammed ◽  
A.S. Adamu ◽  
S.R. Abdullahi ◽  
G.M. Salihu ◽  
...  

Gastrointestinal tract (GIT) in human plays an important role in providing habitat to various microbial communities including numerous prokaryotic cells numbering over 100 trillion. This study was aimed at accessing the multidrug resistance (MDR) of E. coli among patients with GIT infections seeking health care at Dalhatu Araf Specialist Hospital (DASH), Lafia, Nasarawa state, Nigeria. A total of one hundred and fifty (150) stool samples were collected using sterile sample containers from patients with symptoms of GIT infections in search of medical care in this hospital. The samples collected were processed using standard microbiological methods. Out of the total samples collected, 35.3% (53/150) revealed the presence of E. coli whereas 64.7% did not. Extended spectrum beta-lactamases (ESBL) were found in 45.3% of E. coli obtained in this study, which were further used for the antimicrobial susceptibility test. E. coli were more resistant to ampicillin (75.51%), followed by amoxycillin/clavulanic acid (61.22%) and were least resistant to imipenems (12.25%). In conclusion, MDR E. coli producing ESBL are abundant among patients with GIT infections visiting DASH, Lafia. Thus, it is important to determine the genes responsible for this MDR as well as dissemination of adequate information to the masses visiting the hospital for treatment on the possible effects caused by the antibiotics abuse Keywords: Escherichia coli, Multiple-Drug Resistance, Antimicrobial, Gastrointestinal tract, Extended Spectrum Beta-Lactamases, Microbiological methods


1999 ◽  
Vol 27 (2) ◽  
pp. 203-203
Author(s):  
Kendra Carlson

The Supreme Court of California held, in Delaney v. Baker, 82 Cal. Rptr. 2d 610 (1999), that the heightened remedies available under the Elder Abuse Act (Act), Cal. Welf. & Inst. Code, §§ 15657,15657.2 (West 1998), apply to health care providers who engage in reckless neglect of an elder adult. The court interpreted two sections of the Act: (1) section 15657, which provides for enhanced remedies for reckless neglect; and (2) section 15657.2, which limits recovery for actions based on “professional negligence.” The court held that reckless neglect is distinct from professional negligence and therefore the restrictions on remedies against health care providers for professional negligence are inapplicable.Kay Delaney sued Meadowood, a skilled nursing facility (SNF), after a resident, her mother, died. Evidence at trial indicated that Rose Wallien, the decedent, was left lying in her own urine and feces for extended periods of time and had stage I11 and IV pressure sores on her ankles, feet, and buttocks at the time of her death.


1996 ◽  
Vol 24 (3) ◽  
pp. 274-275
Author(s):  
O. Lawrence ◽  
J.D. Gostin

In the summer of 1979, a group of experts on law, medicine, and ethics assembled in Siracusa, Sicily, under the auspices of the International Commission of Jurists and the International Institute of Higher Studies in Criminal Science, to draft guidelines on the rights of persons with mental illness. Sitting across the table from me was a quiet, proud man of distinctive intelligence, William J. Curran, Frances Glessner Lee Professor of Legal Medicine at Harvard University. Professor Curran was one of the principal drafters of those guidelines. Many years later in 1991, after several subsequent re-drafts by United Nations (U.N.) Rapporteur Erica-Irene Daes, the text was adopted by the U.N. General Assembly as the Principles for the Protection of Persons with Mental Illness and for the Improvement of Mental Health Care. This was the kind of remarkable achievement in the field of law and medicine that Professor Curran repeated throughout his distinguished career.


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