Microbiology of the food chain. Sampling techniques for microbiological analysis of food and feed samples

2015 ◽  
Keyword(s):  
Food Chain ◽  
Sampling Techniques ◽  
Microbiological Analysis ◽  
Food And Feed ◽  
Feed Samples

A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA

2021 ◽  
Vol 368 (6) ◽  
Author(s):  
Liwen Zhang ◽  
Qingyu Lv ◽  
Yuling Zheng ◽  
Xuan Chen ◽  
Decong Kong ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Accurate Method ◽  
Detection Methods ◽  
Cereal Crops ◽  
Detection Range ◽  
Highly Sensitive ◽  
Food And Feed ◽  
Good Repeatability ◽  
Feed Samples ◽  
Better Than

ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03–500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.

A multicomponent method for Fusarium toxins in cereal based food and feed samples using HPLC-MS/MS

2005 ◽  
Vol 21 (1) ◽  
pp. 18-22 ◽  
Author(s):  
S. Biselli ◽  
H. Wegner ◽  
C. Hummert
Keyword(s):  
Fusarium Toxins ◽  
Food And Feed ◽  
Feed Samples

scholarly journals Inventory of microbial species with a rationale: a comparison of the IDF/EFFCA inventory of microbial food cultures with the EFSA Biohazard Panel qualified presumption of safety

2019 ◽  
Vol 366 (Supplement_1) ◽  
pp. i83-i88
Author(s):  
F Bourdichon ◽  
S Laulund ◽  
P Tenning
Keyword(s):  
Risk Assessment ◽  
Food Safety ◽  
Food Chain ◽  
European Food Safety Authority ◽  
Human Consumption ◽  
Microbial Species ◽  
Food Cultures ◽  
Food And Feed ◽  
Microbial Strains ◽  
International Dairy Federation

ABSTRACT In order to provide a harmonised preassessment to support risk assessment performed by the European Food Safety Authority (EFSA), the Biohazard Panel in 2007 published guidelines for evaluation of the safety of a strain included in the food chain, the Qualified Presumption of Safety (QPS). Since 2008, the Biohazard Panel has published on a regular basis an update of the microbial strains submitted for approval and extends the list of species which have been granted QPS status. The International Dairy Federation (IDF) and the European Food and Feed Cultures Association (EFFCA) have, since 2002, been conducting a project on the safety demonstration of microbial food cultures (MFCs). Following the publication of IDF Bulletin 377–2002, an inventory of MFCs was published in IDF Bulletin 455–2012 and updated most recently in IDF Bulletin 495–2018. These two lists developed by EFSA (QPS) and IDF/EFFCA both propose as an outcome an inventory of microbial species that are safe for human consumption. To avoid confusion when these two inventories are compared, this review attempts to explain the rationale that was used to develop them and explain how the two lists should be understood.

Way forward to bring insects in the human food chain

2015 ◽  
Vol 1 (2) ◽  
pp. 121-129 ◽  
Author(s):  
P. Vantomme
Keyword(s):  
Food Security ◽  
Environmental Impacts ◽  
Food Chain ◽  
National Policy ◽  
Economic Benefits ◽  
Special Focus ◽  
Health Food ◽  
Environmental Consequences ◽  
Food And Feed ◽  
Legal Frameworks

The recognition that insects can contribute to food security has received a remarkable boost by the media, and is getting more attention from researchers and from food and feed agencies worldwide. This paper reviews major activities and achievements over the past 4 years and suggests ways forward mainly from an FAO perspective. To assure that insects can remain part of the diets of 2 billion people, action is urgently needed to protect and ensure sustainability in gathering wild insect populations, to promote simple semi-domestication techniques, and to farm insects at household or industrial scale levels. Integrating edible insects as healthy food into the agenda and campaigns of food agencies will require a more comprehensive understanding on the nutritional and health values of more insect species. Further investigation is needed particularly of impacts on consumer's health, food safety, environmental impacts and risk assessments of using insects in the food chain. National and international poverty alleviation agencies and aid programmes need to be made aware that gathering and farming insects is a viable option to help people improve their livelihoods. For this to happen, a comprehensive awareness raising campaign needs to be launched on the socio-economic benefits that insect gathering and farming can offer, with a special focus on improving the food security of the poorest of society. Legislators and policy makers are called on to deliberate and include insects as feed and food into existing national policy and legal frameworks covering the food, health and feed sectors. Research is needed to investigate the sustainability and quantify the environmental impacts of harvesting and farming insects as compared with traditional farming and livestock-raising practices. This would help to inform the public about the real footprint and cost of our food choices and on their socio-economic and environmental consequences.

scholarly journals Evaluation of an alternative spectroscopic approach for aflatoxin analysis: Comparative analysis of food and feed samples with UPLC–MS/MS

2017 ◽  
Vol 239 ◽  
pp. 1087-1097 ◽  
Author(s):  
Katrina Campbell ◽  
Ana L. Ferreira Cavalcante ◽  
Pamela Galvin-King ◽  
Michalina Oplatowska-Stachowiak ◽  
Catherine Brabet ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Food And Feed ◽  
Feed Samples
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